Search Results (8)

The root system of clonal rootstocks has been poorly studied, despite its crucial importance. Roots not only provide support and nutrition to the plant but also contribute to tolerance to pests, diseases and environmental stresses, in addition to optimizing yields. Although the initial cost of clonal rootstocks is higher, the investment is offset by the reduction in phytosanitary treatments, greater longevity of the trees and a lower mortality rate. The aim of this research was to quantify the root system growth of five clonally propagated dwarf and normal avocado genotypes evaluated in rhizotrons, with the perspective of identifying distinctive characteristics suitable for their possible use in container culture. The avocado clonal plants to be evaluated were placed in rhizotrons and evaluated for six months, where 35 growth variables were evaluated with the aid of a root analyzer program. A randomized complete block design with five treatments (genotypes) and three replications was used under greenhouse conditions. Analysis of variance, variable purge and multivariate analysis were performed. It was found that ‘Duke 7’ and ‘San Martín’ were statistically different for most of variables, with ‘San Martín’ showing less root growth and ‘Duke 7’ showing remarkable lateral growth. The adventitious root system’s growth depends on the genotype, and the use of rhizotrons allows its study, which proved to be a useful methodology for this type of evaluation since it allows the visualization and adequate quantification of root growth. Genotypes with less root growth may be suitable for use in container culture, and roots with extensive lateral growth may be useful in shallow soils. Full article

In Measure for Measure Shakespeare addresses a question that is both straightforward and hard to answer: how do we make people obey the law? Over the course of the play, this simple question gives way to a complex set of problems about human will, political legitimacy, and the origins of sovereign power. Measure for Measure is concerned with illicit activity and ineffective government. But in this comedy—this “problem play”—Shakespeare is especially interested in the political mechanism by which authority and obedience are restored. How is a delinquent population, used to license, brought under control? Shakespeare examines one strategy in this play, one he has seemingly adapted from the Florentine political theorist Niccolò Machiavelli. Multiple critics have recognized that the story of Duke Vincentio and his deviant deputy, Lord Angelo, bear a striking resemblance to the story Machiavelli tells about Cesare Borgia and Remirro de Orco in Chapter 7 of The Prince. Here, I build upon these analyses to offer a new account of Shakespeare’s relationship to Machiavelli and political realism more generally. The Cesare story provides Shakespeare with an opportunity to explore how spectacle and theatricality can be used—not only to subdue an unruly population but to legitimate sovereign authority. However, Shakespeare delves deeper than Machiavelli into the mechanism whereby political authority is reestablished, first by considering the psychological conditions of the Duke’s subjects (both before and during his spectacular display of power), and second, by emphasizing the need for individual citizens to will sovereign authority into being. As we will see, in Shakespeare’s Vienna, order can only be restored once the delinquent people beg to be governed. Full article

Because of their tolerance to root rot and many abiotic stresses, rootstocks are generally required for commercial avocado production. However, cutting and air-layering, which are popular methods of vegetative propagation for producing large numbers of uniform and genetically identical plants, have been unsuccessful for years. To develop a practical and efficient rooting procedure for selected avocado clonal rootstocks, the present research investigated the effects of various factors on rooting cutting. Shoots were divided into three groups (air layering, direct cutting, and stem-buried etiolation) and treated with different combinations (plant growth regulators, PGRs; soaking time; and culture media), in which orthogonal or randomized-block designs were used. The rooting rate, average root length, average root number, average root dry weight, and rooting quality (Q value) were used as evaluation indicators. The results show that etiolation treatment of the mother shoot is the requisite condition for avocado shoot rooting. In addition, the rooting effect of etiolated cuttings was strongly impacted by PGR type, concentration, and soaking time, whereas no significant differences were detected among the culture media. Among these factors, the roots and the survival rates of etiolated cuttings of two hard-to-root varieties were optimal under the following conditions: indolebutyric acid concentration of 2500 mg/L, dipping duration for 5 s, and perlite:vermiculite culture at 1:1. The rooting rate of ‘Dusa’ was generally greater than that of ‘Duke 7’ and reached 82%. Full article

Colorectal cancer (CRC) represents a substantial burden on global healthcare, contributing to significant morbidity and mortality worldwide. Despite advances in screening methodologies, its incidence remains high, necessitating continued efforts in early detection and treatment. Neoplastic invasion and metastasis are primary determinants of CRC lethality, emphasizing the urgency of understanding underlying mechanisms to develop effective therapeutic strategies. This study aimed to explore the potential of serum biomarkers in predicting survival outcomes in CRC patients, with a focus on cathepsin B (CB), leukocytic elastase (LE), total sialic acid (TSA), lipid-associated sialic acid (LASA), antitrypsin activity (ATA), C-reactive protein (CRP), and cystatin C (CC). We recruited 185 CRC patients and 35 healthy controls, assessing demographic variables, tumor characteristics, and 7 serum biomarker levels, including (1) CB, (2) LE, (3) TSA, (4) LASA, (5) ATA, (6) CRP, and (7) CC. Statistical analyses included ANOVA with Tukey’s post hoc tests and MANOVA for continuous variables. Student’s t-test was used for dependent samples, while non-parametric tests like Mann–Whitney U and Wilcoxon signed-rank tests were applied for variables deviating from the normal distribution. Categorical variables were assessed using chi-square and Kruskal-Wallis tests. Spearman’s rank correlation coefficient was utilized to examine variable correlations. Survival analysis employed the Kaplan–Meier method with a log-rank test for comparing survival times between groups. Significant associations were observed between CB (p = 0.04), LE (p = 0.01), and TSA (p = 0.008) levels and survival outcomes in CRC patients. Dukes’ classification stages also showed a significant correlation with survival (p = 0.001). However, no significant associations were found for LASA, ATA, CRP, and CC. Multivariate analysis of LE, TSA, and ATA demonstrated a notable correlation with survival (p = 0.041), notwithstanding ATA’s lack of significance in univariate analysis (p = 0.13). CB, LE, and TSA emerged as promising diagnostic markers with prognostic value in CRC, potentially aiding in early diagnosis and treatment planning. Further research is needed to validate these findings and explore additional prognostic indicators. Full article

One of the best sources of antioxidant and health-promoting bioactive substances is the fruit of V. corymbosum. A potent oxidizing agent, ozone (O₃), can effectively eliminate bacteria. The application of ozone gas to V. corymbosum fruit during storage had a favorable impact on the fruit’s phenolic component and sugar content in the current investigation. After 7 days of storage, phenolic content in all highbush blueberry cultivars and clones tested increased on average by 28.60%, including anthocyanins by 34%. After 14 days of storage, an average increase of 16.50% in phenolic compounds was observed, including a 20.53% increase in anthocyanins. Among all the tested varieties, clone BOR-21 treated with a dose of 0.01 mL·L⁻¹ ozone for 30 min after 14 days had the highest TPC—143.73 mg·100 g⁻¹ f.w. The sugar content of berries treated with a dose of 0.01 mL·L⁻¹ ozone for 30 min, on day 7 and day 14 of storage increased by 9.2% and 6.3%, respectively. On day 7, the highest amount of total sugar (22.74 g·100 g⁻¹) was observed in Duke cultivar after being exposed to 0.01 mL·L⁻¹ ozone for 15 min. The ozonation treatments enhanced the fruit’s saturation with nutrients, which raises the fruit’s value as food. Full article

The choice of rootstock has a significant impact, not only on fruit growth and development, but also on avocado fruit quality and postharvest performance. The objective of this study was to evaluate and compare attributes related to the postharvest quality of Hass avocados from “Mexicola” and “Duke 7” rootstocks grown under similar conditions. This study included two harvests: early (23–26% dry matter) and middle (>26–30% dry matter) per season for the 2016/2017 and 2017/2018 seasons and two storage conditions (regular air (RA) at 5 °C and controlled atmosphere (CA) at 4 kPa O₂ and 6 kPa CO₂ at 5 °C) for 55 days. The results showed significant differences in firmness, color and vascular and flesh browning between storage conditions; in addition to these last three attributes, the rootstock played an important role. The fatty acid profile did not reveal significant differences between fruit from both rootstocks. Finally, the polar metabolite profiles revealed differences only for the storage condition, not associated to the rootstock, which could affect the postharvest performance of Hass avocado fruit. This study is one of the few available showing the interaction of rootstock/Hass cultivar on fruit quality and postharvest performance. Full article

Salinity is one of the major factors limiting avocado yield, primarily due to the high concentration of ions in irrigation water. An experiment was conducted on 2 year old avocado plants (Persea americana Mill.) cv. Hass, grafted onto Duke 7 clonal rootstock growing in pots, to determine the effect of salt stress on growth, as well as physiological and biochemical responses, and the effect of seaweed extract (Ascophyllum nodosum) on salinity stress. Treatments consisted of different types of irrigation water: distilled water, 9 mM NaCl water, distilled water + 2.25 mL of seaweed extract, 9 mM NaCl water + 2.25 mL of seaweed extract and, 9 mM NaCl water + 1.5 mL of seaweed extract. The irrigation treatment was applied every 15 days for 8 months. Treatments with salt reduced plant growth by approximately 50% of the fresh weight of all avocado plant tissues. Seaweed extract reduced the effects of abiotic stress only at an early stage, and increased potassium (K) and calcium (Ca) concentrations in leaves. Full article

Background: Women with a predisposition for breast cancer require a tailored screening program for early cancer detection. We evaluated the performance of mammography (MG), ultrasonography (US), and magnetic resonance imaging (MRI) screening in these women. Patients and Methods: In asymptomatic women either confirmed as BRCA1/2 carriers, or having a greater than 30% probability of being so as estimated by BRCAPRO [Berry D, Parmigiani G. Duke SPORE (Specialized Program of Research Excellence) in Breast Cancer. 1999], we conducted a prospective comparative trial consisting of annual MRI and MG, and biannual US and clinical breast examination. All evaluations were done within 30 days of one another. For each screening round, imaging tests were independently interpreted by three radiologists. Results: The study enrolled 184 women, and 387 screening rounds were performed, detecting 12 cancers (9 infiltrating, 3 in situ), for an overall cancer yield of 6.5%. At diagnosis, 7 infiltrating cancers were smaller than 2 cm (T1); only 1 woman presented with axillary nodal metastases. All tumours were negative for the human epidermal growth factor receptor 2. Of the 12 cancers, MRI detected 10, and MG, 7; US did not identify any additional cancers. The overall recall rate after MRI was 21.8%, as compared with 11.4% for US and 16.1% for MG. Recall rates declined with successive screening rounds. In total, 45 biopsies were performed: 21 as a result of an US abnormality; 17, because of an MRI lesion; and 7, because of a MG anomaly. Interpretation: In high-risk women, MRI offers the best sensitivity for breast cancer screening. The combination of yearly MRI and MG reached a negative predictive value of 100%. The recall rate is greatest with MRI, but declines for all modalities with successive screening rounds. Full article