Search Results (59)

The effects of stocking density (SD) and feeder access (FA) on the performance and damaging behaviour of weaned piglets are not well understood. Three experiments (Ts) were conducted. T1 compared low SD (LSD) (0.55 m²/piglet) and moderate SD (MSD) (0.37 m²/piglet), T2 compared low FA (LFA1) (1.33 piglets/feeder; partially slatted floor) and high FA (HFA1) (0.66 piglets/feeder; fully slatted floor), and T3 compared LFA2 (2.33 piglets/feeder) and HFA2 (1.00 piglets/feeder). T1 included 24 replicates, while T2 and T3 each had 12 replicates. In total, 576 piglets were weighed at days (d) 0, 14, and 35 post-weaning (PW). Faecal consistency (FC) was recorded multiple times per week. Tail and ear injuries were scored at d35 PW. Piglets housed under LSD grew faster throughout the experiment (p < 0.05) and had a lower feed conversion ratio (FCR) between d0 and 14 PW (p = 0.02) than those in MSD. Tail injuries were not significantly affected (p = 0.14). HFA1 piglets had higher FC between d0 and 14 PW (p = 0.01) and overall (p = 0.02), but fewer intact tails at d35 PW (p = 0.01) compared with LFA1. In T3, LFA2 piglets had a lower average daily gain (p = 0.03), higher FCR between d14 and 35 PW (p = 0.04), and fewer intact tails (p < 0.01) than HFA2 piglets. These results suggest that both FA and SD can influence piglet performance, FC, and damaging behaviour. Full article

Background/Objectives: D-lysergic acid diethylamide (D-LSD) is under investigation as a potential therapeutic strategy for alcohol use disorder (AUD). However, the extreme light sensitivity of D-LSD presents a significant challenge in developing suitable pharmaceutical forms, particularly for clinical trial settings. This study proposes a liquid-filled capsule formulation designed to provide accurate dosing while protecting D-LSD from photodegradation. Methods: To support formulation development and ensure its suitability as an investigational medicinal product, a multi-tiered analytical strategy was employed. This included liquid chromatography coupled with ion mobility spectrometry and mass spectrometry (LC-IM-MS), along with quantum chemical calculations (density functional theory (DFT) and time dependent-DFT (TD-DFT)), to ensure robust and orthogonal structural characterization of degradation products. Results: Photostress studies demonstrated that while D-LSD in solution rapidly degrades into photoisomers and photooxidative byproducts, the capsule formulation markedly mitigates these transformations under ICH-compliant conditions. Conclusions: These findings highlight the essential role of orthogonal stability profiling in guiding formulation development and demonstrate that this approach may offer a viable, photostable platform for future clinical investigation of D-LSD in the treatment of AUD. Full article

Background/Objectives: Degenerative lumbar spine disease (DLSD) is increasingly managed with minimally invasive surgery (MIS) and evolving anesthesia methods. While general anesthesia (GA) remains standard, spinal anesthesia (SA) may offer faster recovery and fewer side effects. This study compares the clinical and economic outcomes of GA versus SA in transforaminal lumbar interbody fusion (TLIF). Methods: A retrospective review of 18 TLIF patients (2018–2022) was performed, with 9 patients in each cohort. Patients were matched by demographics and procedure type. Data collected included operative time, blood loss, complications, postoperative opioid utilization, and 30-day readmissions. Costs were analyzed in categories: anesthesia, implants, inpatient care, operating room (OR) supplies, OR time, and PACU fees, using Wilcoxon Rank T-tests and Pearson Chi-Squared tests. Results: Clinical outcomes such as blood loss, and operative time were similar between groups. However, SA patients had significantly shorter LOS compared to GA (SA: 12 h vs. GA: 84 h, % difference: −150%, p = 0.04). Additionally, SA patients had lower total direct costs ($27,881.85 vs. $35,669.01; p = 0.027). Significant cost reductions with SA were noted in OR supplies/medications ($7367.93 vs. $10,879.46; p = 0.039) and inpatient costs ($621.65 vs. $3092.66; p = 0.027). Within these categories, reductions were observed for intravenous solutions, sedatives/anesthetics, pressure management, labs, imaging, evaluations, hospital care, and medications. Although costs for implants, anesthesia care, OR time, and PACU fees were lower with SA, these differences did not reach statistical significance. Conclusions: In TLIF for DLSD, SA provides significant economic advantages over GA while yielding comparable clinical outcomes. These results support SA as a cost-effective alternative, warranting further prospective studies to confirm these findings. Full article

The allelic diversity within genes controlling the vernalization requirement (VRN1) and photoperiod response (PPD1) determines the ability of wheat to adapt to a wide range of environmental conditions and influences grain yield. In this study, allelic variations at the VRN-A1, VRN-B1, VRN-D1 and PPD-D1 genes were studied for 89 accessions of Triticum compactum from different eco-geographical regions of the world. The collection was evaluated for heading date in both field and greenhouse experiments under a long photoperiod and without vernalization. Based on heading date characteristics, 52 (58.4%) of the genotypes had a spring growth habit, and all of them carried at least one dominant VRN1 allele, while 37 (41.6%) accessions had a winter growth habit and carried the triple recessive allele combination. The photoperiod-sensitive Ppd-D1b allele was detected in 85 (95.5%) accessions and the insensitive Ppd-D1a allele in four (4.5%) accessions. A total of 10 phenology gene profiles (haplotypes) were observed at four major genes in the T. compactum germplasm collection. The LSD test revealed significant differences in the mean heading date among the different spring phenology gene profiles, both in greenhouse and field conditions. In addition, 21 microsatellite markers (simple sequence repeats, SSRs) were used to assess the genetic diversity in the collection. The 21 SSR markers amplified a total of 183 alleles across all the genotypes, with a mean of 3.2 alleles per locus. The polymorphic information content ranged from 0.49 to 0.94, with a mean of 0.84. The results of this study may be useful for both T. compactum and common wheat breeding programs as a source of agronomic traits. Full article

Degenerative changes are characterized by the formation of vertebral osteophytes, the hypertrophy of facet joints, and narrowing of the intervertebral space. This study aimed to investigate the concentrations of trace elements (Al, As, Se, Zn, Fe, Mo, Cu) in spinal tissues (intervertebral discs, muscle, and bone) of patients with degenerative lumbar spine disease (DLSD) and their potential associations with the disease. The research involved 13 patients undergoing surgery for symptomatic degenerative spine disease. The trace element concentrations were analyzed using chemical and radiographic assessments, with a statistical analysis performed through a Mann–Whitney U-test, Spearman’s rank correlation test, principal component analysis (PCA), and canonical discriminant analysis (CDA). The results showed significant variations and correlations among the trace elements across different spinal tissues, suggesting their roles in metabolic and oxidative processes and the pathology of spinal degeneration. Full article

Sichuan pepper is known for its unique aroma and tingling, numbing sensation, making it a key ingredient in Sichuan cuisine. This study explored the effect of harvest periods on the quality of Sichuan pepper at five selected harvest periods (LSA (early harvest), LSB, LSC, LSD, and LSE (late harvest)) along the 24 solar terms in the traditional Chinese lunar calendar. Apart from evaluating their physicochemical and volatile profiles, the growth characteristics, polyphenol and flavonoid contents, antioxidant properties, and free amino acid and other nutrient concentrations in these peppers were also analysed. Results showed that the moisture content, weight, and shape of Sichuan peppers improved progressively, peaking at the LSE harvest period. Throughout maturation, the energy content of the Sichuan pepper remained stable. Polyphenols and flavonoids, indicators of antioxidant capacity, increased in later harvest periods. A total of 18 amino acids were detected in Sichuan pepper. Proline was the most abundant amino acid, followed by serine, arginine, and glutamic acid, accounting for 83% of the total amino acids. Based on the taste threshold values of amino acids, a taste activity value (TAV) analysis of the amino acids was conducted. The TAV analysis of arginine and glutamic acid were greater than 1, indicating their significant contribution to the bitterness and umami taste, respectively. Through the principal component analysis of the electronic tongue, it was found that Sichuan pepper picked in late July (LSA stage) had a significant difference from that picked in September (LSD and LSE stages), while the difference in taste characteristics between Sichuan pepper in early September and late September was relatively small. Terpenes were the primary volatile compounds, and the number of compounds increased as the harvest period was delayed. PLS-DA analysis revealed that D-limonene had the highest VIP value, indicating its significant contribution to the overall odour of Sichuan pepper, and thus can serve as an indicator for assessing the maturity of Sichuan pepper. This study offers valuable insights for optimising the harvesting period of Sichuan pepper and serves as a theoretical reference for enhancing the development of the seasoning industry. Full article

Background and Objectives: Hind limb ischemia–reperfusion (I/R) injury is a serious clinical condition that requires urgent treatment and develops as a result of a sudden decrease in blood flow in the extremity. Antioxidant combinations are frequently used in diseases today. This study aimed to investigate and compare the effectiveness of ellagic acid (EA) and berberine (BER), which are important antioxidants, and the combination on hind limb I/R injury to evaluate their therapeutic power. Materials and Methods: Thirty-five male Sprague Dawley rats were randomly divided into five groups: sham, I/R, EA+I/R, BER+I/R, and EA/BER+I/R. In the I/R procedure, the infrarenal abdominal aorta was clamped and reperfused for 2 h. EA (100 mg/kg, ip) and BER (200 mg/kg, ip) were administered in the 75th minute of ischemia. Oxidative stress markers (MDA, GSH, SOD, and CAT) and TNF-α were measured. Apoptosis (Bax, Bcl-2, and Cleaved caspase-3) and pyroptosis (Nrf2, NLRP3, and Gasdermin D) pathways were evaluated via Western blot. Muscle tissue was examined histopathologically by hematoxylin eosin staining. One-way ANOVA and post hoc LSD tests were applied for statistical analyses (p < 0.05). Results: Bax levels increased in the ischemia group and decreased with EA and BER (p < 0.05). Bcl-2 levels decreased in the ischemia group but increased with EA and BER (p < 0.05). The highest level of the Bax/Bcl-2 ratio was in the I/R group (p < 0.05). Cleaved caspase 3 was higher in the other groups compared to the sham group (p < 0.05). While Nrf2 decreased in the I/R group, NLRP3 and Gasdermin D increased; EA and BER normalized these levels (p < 0.05). In the histopathological analysis, a combination of EA and BER reduced damage (p < 0.05). TNF-α levels were similar between groups (p > 0.05). MDA levels were reduced by EA and BER, but GSH, SOD, and CAT levels were increased (p < 0.05). Conclusions: It was concluded that TNF-α levels depend on the degree and duration of inflammation and that no difference was found in relation to duration in this study. As a result, EA, BER, and their combination could be potential treatment agents on hind limb I/R injury with these positive effects. Full article

Background/Objectives: Suicide accounts for 1.4% of global deaths, and the slow-acting nature of traditional treatments for suicide risk underscores the need for alternatives. Psychedelic therapies may rapidly reduce suicide risk. This systematic review evaluates impact of psychedelic therapies on suicide-related outcomes. Methods: A systematic search of MEDLINE, Embase, PsycINFO, and ClinicalTrials.gov was conducted up to November 2024. Results: Four randomized controlled trials (RCTs) evaluated suicidality as a secondary outcome or safety measure, showing significant reductions in suicidal ideation with psilocybin (three studies) and MDMA-assisted therapy (MDMA-AT; one study). Effect sizes, measured by Cohen’s d, ranged from =0.52 to 1.25 (p = 0.01 to 0.005), with no safety issues reported. Five additional RCTs assessed suicidality as a safety measure, showing reductions in suicidal ideation with psilocybin (two studies) and MDMA-AT (three studies; p = 0.02 to 0.04). Among 24 non-randomized and cross-sectional studies, results were mixed. Psilocybin (three studies) reduced suicidal ideation, with odds ratios (OR) of 0.40–0.75. MDMA-AT (five studies in PTSD patients) had a pooled effect size of d = 0.61 (95% CI: 0.32–0.89). LSD (six studies) showed increased odds of suicidality, with odds ratios ranging from 1.15 to 2.08. Studies involving DMT (two studies) and multiple psychedelics (three studies) showed mixed results, with DMT studies not showing significant effects on suicidality and studies involving multiple psychedelics showing varying outcomes, some reporting reductions in suicidal ideation and others showing no significant change. Conclusions: The effect of psychedelic therapies on suicide-related outcomes remains inconclusive, highlighting the need for further trials to clarify safety and therapeutic mechanisms. Full article

Snapping Shrimps (SSs) live in warm marine areas. Snapping Shrimps Noise (SSN), loud sounds generated by these underwater creatures, serves as a major source of in performance degradation by decreasing the Signal-to-Noise Ratio (SNR) for underwater acoustic communication and target detection. Thus, we propose a unified solution for SSN detection and reduction in this paper. First, Signal Presence Probability (SPP) is calculated for SSN detection, and then the SPP is provided to Non-negative Matrix Factorization (NMF) as a weight for SSN reduction. In the proposed method, SPP acts as a key factor for SSN detection and reduction. To verify the effectiveness of the proposed method, the SAVEX-15 dataset, real ocean data containing SSN, is used. As a result of SSN detection, it was confirmed that SPP presented the highest performance in the Receiver Operating Characteristics curve, and we achieved 0.014 higher Area Under the Curve compared to competing methods. In addition, Continuous Wave and Linear Frequency Modulation signals were set as target signals and combined with the SAVEX-15 data for evaluation of noise reduction performance. As a result, the performance of the SPP-weighted NMF (WNMF) presented at least 2 dB higher SNR and SDR while maintaining less LSD compared to the Optimally Modified Log Spectral Amplitude estimator and NMF. Full article

Matched line segments are crucial geometric elements for reconstructing the desired 3D structure in stereo satellite imagery, owing to their advantages in spatial representation, complex shape description, and geometric computation. However, existing line segment matching (LSM) methods face significant challenges in effectively addressing co-linear interference and the misdirection of parallel line segments. To address these issues, this study proposes a “continuous–discrete–continuous” cyclic LSM method, based on the Voronoi diagram, for stereo satellite images. Initially, to compute the discrete line-point matching rate, line segments are discretized using the Bresenham algorithm, and the pyramid histogram of visual words (PHOW) feature is assigned to the line segment points which are detected using the line segment detector (LSD). Next, to obtain continuous matched line segments, the method combines the line segment crossing angle rate with the line-point matching rate, utilizing a soft voting classifier. Finally, local point-line homography models are constructed based on the Voronoi diagram, filtering out misdirected parallel line segments and yielding the final matched line segments. Extensive experiments on the challenging benchmark, WorldView-2 and WorldView-3 satellite image datasets, demonstrate that the proposed method outperforms several state-of-the-art LSM methods. Specifically, the proposed method achieves F1-scores that are 6.22%, 12.60%, and 18.35% higher than those of the best-performing existing LSM method on the three datasets, respectively. Full article

Subclinical necrotic enteritis (NE) is an enteric disease that inflicts significant economic losses in the poultry industry, primarily by reducing performance in commercial flocks but without significant mortality. This study evaluated the effects of a variety of phytogenic blends on broilers’ performance and carcass composition during an induced NE challenge. In this study, 1120 day (d)-old male broilers were allocated to four treatments groups (14 replicate floor pens, 20 birds/pen): the control (CONT) group, fed a basal corn-soybean diet, and three phytogenic blend dietary additives (PHYTO1, PHYTO2, and PHYTO3) added to the basal diet at 150, 250, and 500 mg/kg feed, respectively. Subclinical NE was induced by spraying a concentrated coccidiosis vaccine onto the feed and litter 24 h post-placement. On day 8, two birds/pen were necropsied for NE lesions. On days 8, 14, 28, and 42, the average daily gain (ADG), feed intake (ADFI), and feed conversion ratio (FCR) were calculated. On day 42, two birds/pen were euthanized to assess carcass composition using dual-energy X-ray absorptiometry (DXA). Statistical analyses for all data were performed using the ANOVA procedure (JMP, Pro 16) and significance (p ≤ 0.05) between treatments was determined by the LSD test. There was no effect of treatment on NE lesions. PHYTO1, PHYTO2, and PHYTO3 significantly improved FCR from days 9 to 14, 0 to 14, and 0 to 42 and resulted in greater ADG from days 9 to 14, 29 to 42 and cumulatively on days 0 to 42. Carcass composition data revealed a numerically higher lean-to-fat ratio in the PHYTO groups compared to the CONT group. These results indicate that the dietary supplementation of phytogenic blends could alleviate the adverse effects of NE challenge on broilers’ performance and carcass composition. Full article

Two experiments were conducted to assess the efficiency of including the novel protein ingredient Arthrospira platensis or improved soybean meal in a broiler diet. The first experiment aimed to determine the feeding value of soybean meal produced from varieties of soybeans bred for increased amino acid content (SBAA) and improved oil content (SBO) compared to a conventional soybean variety in an ANOVA design fed to Cobb 500 female broilers for 28–42 d. The SBAA and SBO soybeans contained overall higher amino acid content and lower oligosaccharide content compared to the conventional soybean variety in addition to improved oil quality. The second experiment assessed the novel protein ingredient microalgae, Arthrospira platensis (algae), and was conducted to evaluate algae and corn distillers’ grain (DDGS) inclusion on broiler performance for a 28–42 d feeding period in Cobb CF05 male broilers with a 2 × 2 factorial treatment array. Prior to the experimental period, all birds were reared on common feeds. In Experiment 1, birds were fed a diet containing 20% inclusion of an experimental soybean source in the form of full-fat soybean meal. In Experiment 2, the four dietary treatments consisted of diets containing algae at inclusion levels of either 0 or 2% and DDGS at inclusion levels of 0% and 8%. Diets were fed to 288 female broilers (Experiment 1) and 384 male broilers (Experiment 2), placed in eight replicate pens of twelve birds, and live performance was assessed from d 28 to 42. At d 42, six birds from each pen were randomly selected and processed for evaluation of carcass traits and incidence of woody breast. For Experiment 1, all performance data were analyzed using a one-way ANOVA using JMP Pro 16 software with diet as the fixed effect and block as a random effect. Statistical significance was considered at p ≤ 0.05. No significant responses were observed for any recorded measurement for live performance, carcass traits, or woody breast. All data in Experiment 2 were analyzed as a full factorial with a mixed model using JMP software with algae, DDGS, and algae × DDGS as fixed effects and block as a random effect. The F-protected Fisher’s LSD test was used to separate means when p ≤ 0.05. No significant responses were observed for the algae, DDGS, and algae × DDGS influences on BWG, FI, and FCR or processing characteristics; the ingredient source did not affect bird performance. Experimental soybean lines developed at the University of Arkansas were able to be incorporated into broiler diets without decreasing performance. Algae has the potential to be a protein-contributing ingredient for broilers. Full article

Indoor localization has long been a challenging task due to the complexity and dynamism of indoor environments. This paper proposes ULG-SLAM, a novel unsupervised learning and geometric-based visual SLAM algorithm for robot localizability estimation to improve the accuracy and robustness of visual SLAM. Firstly, a dynamic feature filtering based on unsupervised learning and moving consistency checks is developed to eliminate the features of dynamic objects. Secondly, an improved line feature extraction algorithm based on LSD is proposed to optimize the effect of geometric feature extraction. Thirdly, geometric features are used to optimize localizability estimation, and an adaptive weight model and attention mechanism are built using the method of region delimitation and region growth. Finally, to verify the effectiveness and robustness of localizability estimation, multiple indoor experiments using the EuRoC dataset and TUM RGB-D dataset are conducted. Compared with ORBSLAM2, the experimental results demonstrate that absolute trajectory accuracy can be improved by 95% for equivalent processing speed in walking sequences. In fr3/walking_xyz and fr3/walking_half, ULG-SLAM tracks more trajectories than DS-SLAM, and the ATE RMSE is improved by 36% and 6%, respectively. Furthermore, the improvement in robot localizability over DynaSLAM is noteworthy, coming in at about 11% and 3%, respectively. Full article

Major Depressive Disorder (MDD) is a complex mental disorder that involves alterations in signal transmission across multiple scales and structural abnormalities. The development of effective antidepressants (ADs) has been hindered by the dominance of monoamine hypothesis, resulting in slow progress. Traditional ADs have undesirable traits like delayed onset of action, limited efficacy, and severe side effects. Recently, two categories of fast-acting antidepressant compounds have surfaced, dissociative anesthetics S-ketamine and its metabolites, as well as psychedelics such as lysergic acid diethylamide (LSD). This has led to structural research and drug development of the receptors that they target. This review provides breakthroughs and achievements in the structure of depression-related receptors and novel ADs based on these. Cryo-electron microscopy (cryo-EM) has enabled researchers to identify the structures of membrane receptors, including the N-methyl-D-aspartate receptor (NMDAR) and the 5-hydroxytryptamine 2A (5-HT_2A) receptor. These high-resolution structures can be used for the development of novel ADs using virtual drug screening (VDS). Moreover, the unique antidepressant effects of 5-HT_1A receptors in various brain regions, and the pivotal roles of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) and tyrosine kinase receptor 2 (TrkB) in regulating synaptic plasticity, emphasize their potential as therapeutic targets. Using structural information, a series of highly selective ADs were designed based on the different role of receptors in MDD. These molecules have the favorable characteristics of rapid onset and low adverse drug reactions. This review offers researchers guidance and a methodological framework for the structure-based design of ADs. Full article

Graph distance measures have emerged as an effective tool for evaluating the similarity or dissimilarity between graphs. Recently, there has been a growing trend in the application of movie networks to analyze and understand movie stories. Previous studies focused on computing the distance between individual characters in narratives and identifying the most important ones. Unlike previous techniques, which often relied on representing movie stories through single-layer networks based on characters or keywords, a new multilayer network model was developed to allow a more comprehensive representation of movie stories, including character, keyword, and location aspects. To assess the similarities among movie stories, we propose a methodology that utilizes a multilayer network model and layer-to-layer distance measures. We aim to quantify the similarity between movie networks by verifying two aspects: (i) regarding many components of the movie story and (ii) quantifying the distance between their corresponding movie networks. We tend to explore how five graph distance measures reveal the similarity between movie stories in two aspects: (i) finding the order of similarity among movies within the same genre, and (ii) classifying movie stories based on genre. We select movies from various genres: sci-fi, horror, romance, and comedy. We extract movie stories from movie scripts regarding character, keyword, and location entities to perform this. Then, we compute the distance between movie networks using different methods, such as the network portrait divergence, the network Laplacian spectra descriptor (NetLSD), the network embedding as matrix factorization (NetMF), the Laplacian spectra, and D-measure. The study shows the effectiveness of different methods for identifying similarities among various genres and classifying movies across different genres. The results suggest that the efficiency of an approach on a specific network type depends on its capacity to capture the inherent network structure of that type. We propose incorporating the approach into movie recommendation systems. Full article