Search Results (1,343)

This study presents an optimized and comparative investigation of four intelligent energy management strategies—Proportional–Integral–Derivative (PID), Fuzzy Logic Control (FLC), Equivalent Consumption Minimization Strategy (ECMS), and Artificial Neural Network (ANN)—applied to a photovoltaic–fuel cell–battery hybrid electric vehicle (PV–FC–HEV). A high-fidelity MATLAB/Simulink model integrates a 6 kW proton-exchange membrane fuel cell (PEMFC), a 500 W photovoltaic subsystem with MPPT, and a lithium-ion battery (LiB) pack. While 1000 W/m² represents Standard Test Conditions (STC), the level of 400 W/m² was specifically selected to simulate average cloudy conditions common in urban driving environments, rather than standard NOCT (800 W/m²), to test the EMS’s robustness under significantly reduced PV support and stressed battery conditions (initial SOC = 30%). While surface contamination and the resulting performance degradation significantly impact real-world results, this study assumes a clean surface to establish an idealized performance baseline for the control algorithms. However, the authors acknowledge that contaminant accumulation is a key factor; future work will incorporate a degradation factor (e.g., a 10–15% efficiency penalty) to evaluate the reliability of these EMS strategies under actual operating conditions. ECMS achieved the lowest hydrogen consumption, saving up to 10 L compared with PID, while ANN maintained the most stable state of charge (SOC > 80%), minimizing deep discharge cycles and improving operational stability. FLC provided balanced operation under fluctuating irradiance. Overall, ANN offered the most harmonized energy flow and dynamic stability, whereas ECMS maximized fuel economy. The findings provide practical guidance for designing sustainable and intelligent control systems in next-generation hybrid electric vehicles. Full article

The structural safety of lithium-ion battery systems in electric vehicles (EVs) has become increasingly important with growing concerns over battery-related accidents. In particular, external impacts on the battery pack case (BPC) can cause cell deformation or short-circuiting, potentially leading to thermal runaway. In this study, the mechanical integrity of a commercial BPC was evaluated using both drop-weight impact tests and finite element method (FEM) simulations. A 10 kg hemispherical or cylindrical weight was dropped from a height of 7 m to generate high-energy vertical impacts. The deformation and potential failure of the BPC were examined experimentally, and equivalent FEM simulations were conducted to analyze stress and deformation responses. In both cases, the BPC maintained structural integrity without cracking or intrusion into the battery cell region. The hemispherical impact resulted in relatively shallow, distributed deformation, whereas the cylindrical impact produced more localized indentations due to stress concentrations. The close agreement between experiment and simulation confirms the suitability of FEM for pre-assessment of BPC impact on safety. These findings provide a useful basis for establishing mechanical design criteria and improving battery protection strategies for EV applications. Full article

Hypovolemia is a systemic state characterized by severe reduction in the effective blood volume with subsequent tissue hypoperfusion. It may be due to fluid loss, decreased water intake, fluid redistribution, or systemic disease. The present study aimed to evaluate the diagnostic significance of the renal artery resistive index (RRI), caudal vena cava (CVC) diameter, and aorta (Ao) diameter in dogs with hypovolemia. For this purpose, 30 dogs (hypovolemic, n = 15; control, n = 15) were investigated. Clinical variables and hematological findings were investigated for each dog. Pulsed-wave Doppler ultrasound was performed to measure the RRI and diameters of the CVC and Ao. Ultrasound examination revealed a markedly elevated RRI (p < 0.001) and significantly reduced CVC (p < 0.001) and Ao (p < 0.001) diameters in hypovolemic dogs compared to controls, reflecting increased vascular resistance and impaired venous return. Biochemical analysis showed significant increases in blood urea nitrogen (BUN) and BUN:Cr ratio, while Cr remained unchanged. Hematological variables demonstrated limited diagnostic value, with only mild increases in packed cell volume (PCV%). Correlation analysis confirmed a strong positive correlation between RRI and BUN (r = 0.917; p < 0.01), RRI and BUN:Cr ratio (r = 0.664; p < 0.01), and CVC and Ao diameters (r = 0.832; p < 0.05). Receiver operating characteristic (ROC) analysis with area under the curve (AUC) identified RRI (AUC = 0.99), CVC diameter (AUC = 0.93), and Ao diameter (AUC = 0.88) as highly accurate markers of hypovolemia, whereas the CVC:Ao ratio and hematological markers provided poor discrimination. Logistic regression confirmed significant diagnostic value for RRI, CVC diameter, Ao diameter, and BUN, but final multivariate analysis revealed RRI as the sole independent early diagnostic marker (p < 0.001; OR: 196.0; 95% CI: 11.12–34.72). In conclusion, RRI measured by Doppler ultrasound is the most reliable and sensitive early diagnostic marker for hypovolemia in dogs, outperforming conventional biochemical and hematological markers. Full article

Background: Dengue fever is one of the most common mosquito-borne viral infections, with severe cases characterized by plasma leakage, hemorrhage, and multi-organ involvement. Identification of dengue serotypes and reliable biomarkers is essential for predicting disease progression and guiding timely interventions. Methods: This prospective cohort study was conducted at a super-speciality tertiary care hospital in southern India from July 2024 to July 2025. A total of 69 patients presenting with dengue warning signs were included in the study. Patients were categorized into the severe dengue group (n = 25) and non severe dengue group (n = 44). Clinical data, laboratory findings, dengue serotype, and serial serum samples collected on Days 1, 4, and 8 were analyzed to evaluate the predictive and monitoring efficacy of Interleukin-6 (IL-6) and Interleukin-8 (IL-8), and followed up till discharge. Results: Out of 69 dengue patients with warning signs, 32 dengue-positive patients were serotyped, which included DEN V-1 (31.3%), DEN V-2 (31.3%), DEN V-3 (15.6%), DEN V-4 (18.8%), and mixed DEN V-(2 + 3) (3.1%). Severe dengue patients exhibited a higher frequency of secondary dengue infection (IgG) than primary dengue infection (88% vs. 12%), with statistically significantly higher packed cell volume, hemoglobin levels, high AST levels, and prolonged activated partial thromboplastin time, as well as lower platelet counts and albumin levels. Platelet transfusion was given to 35 dengue patients, which had also resulted in significant length of stay in hospital in comparison to non-transfused patients. IL-6 and IL-8 levels were significantly elevated in severe dengue patients when compared to non-severe dengue patients on Day 1 and Day 4, followed by a decline on Day 8, corresponding with clinical recovery. However, the elevated IL-8 levels were observed to be significantly associated with longer hospital stays, indicating its potential role as an early predictor of disease progression. Conclusions: The observed co-circulation of multiple serotypes reflects the hyper-endemic pattern reported across India. Early measurement of these cytokines IL-6 and IL-8 helps distinguish severe from non-severe dengue among patients presenting with warning signs. IL-6 and IL-8 may have potential as biomarkers for disease severity. However their role in guiding platelet transfusion requires further investigation in non-severe cases and prioritizing timely management for those at higher risk of severe disease. Full article

Background: Hepatitis B virus (HBV) infection continues to be a major public health concern, especially in sub-Saharan Africa, where widespread epidemics and restricted availability of long-term antiviral therapies result in higher mortality and morbidity rates. Drug repurposing represents a strategic approach to accelerate the discovery of effective therapies by leveraging agents with demonstrated antiviral and immunomodulatory activity. Product Nkabinde (PN) is a patented African polyherbal formulation initially developed for the treatment of HIV. Recent experimental studies demonstrate PN’s potent anti-HIV activity and significant immunomodulatory effects in human immune cells, implicating host-directed mechanisms relevant to chronic viral infections. This study combines an integrative application of network pharmacology and molecular docking to evaluate the repurposing potential of PN as a multi-target agent in HBV. Method: Bioactive components of PN were screened, and compound-associated targets were intersected with HBV-associated genes (proteins) to construct a protein–protein interaction (PPI) network. Topological analysis identified 10 hub targets (STAT1, STAT3, SRC, HCK, EGFR, SYK, PIK3CA, PIK3CB, PIK3R1, and PTPN11). Gene Ontology and KEGG pathway enrichment were performed with an FDR cut-off < 0.05. Significantly enriched pathways included JAK–STAT signaling, chemokine signaling, EGFR-TKI resistance, PI3K complex signaling, and viral infection pathways, particularly those related to Kaposi sarcoma virus and HSV-1, indicating immunoregulatory and antiviral roles. Molecular docking was performed using AutoDock Vina 1.1.2 to evaluate binding affinity and interaction mode of key PN phytochemicals against the hub proteins, and results were compared to their respective co-crystallized ligands. Results: Molecular docking indicated that major phytochemicals from PN exhibited significant binding affinities across all 10 hub host targets, typically outperforming or closely matching their respective co-crystallized ligands. The strongest contacts were observed for β-sitosterol–PIK3CB (−14.2 kcal/mol) and oleanolic acid–SYK (−14.0 kcal/mol), which were significantly stronger than the co-crystallized ligands (−7.9 and −8.3 kcal/mol, respectively), indicating robust stabilization within catalytic and regulatory pockets. Procyanidin B2 toward HCK (−10.5 vs. −7.9 kcal/mol) and PIK3CA (−9.5 vs. −7.3 kcal/mol), quercetin toward PIK3R1 (−10.6 vs. −8.2 kcal/mol) and PTPN11 (−9.2 vs. −7.5 kcal/mol), rutin toward SRC (−10.5 vs. 7.8 kcal/mol), and diosgenin toward EGFR (−9.4 vs. 8.4 kcal/mol). Procyanidin B2 maintained robust multi-hydrogen bonding networks, demonstrating significant binding, despite STAT1 and STAT3 docking showing identical affinities to co-crystals. Conserved hydrogen bonds, π–cation interactions, and significant hydrophobic packing at ATP-binding clefts and regulatory domains supported these interaction patterns, indicating competitive suppression of host signaling nodes taken over by HBV. Conclusions: Together, these results demonstrate that the components of PN possess strong multitarget binding capabilities across the PI3K/AKT, JAK–STAT, SRC-family kinase, EGFR, and SYK pathways, supporting their potential repurposing as host-directed HBV therapeutics with the ability to impede immune evasion, viral persistence, and HBV-associated oncogenic progression. Full article

Thermal management plays a critical role in maintaining the safety and reliability of lithium-ion batteries by limiting excessive temperature rise and reducing non-uniform temperature distribution within battery packs. This study proposes a geometry-driven multi-objective optimization framework for a serpentine liquid-cooling channel to enhance the thermal behavior of a battery module under fixed operating conditions. A three-dimensional computational fluid dynamics (CFD) model was developed for a 40-cell battery module, and Latin hypercube sampling was employed to generate training data for Gaussian Process Regression (GPR) surrogate models. Three geometric design variables, namely, channel thickness (t_c), wall thickness (t_w), and contact surface angle (θ), were considered, while the maximum battery temperature (T_max) and the maximum temperature difference within the battery pack (ΔT_max) were selected as optimization objectives. Sensitivity analysis showed that wall thickness was the dominant parameter, contributing 65.41% and 64.77% to the variations in T_max and ΔT_max, respectively, followed by channel thickness, whereas the influence of the contact surface angle was comparatively limited. The trained GPR models were then coupled with the non-dominated sorting genetic algorithm (NSGA-II) to identify the optimal channel geometry. The optimal design was obtained at t_c = 2.95 mm, t_w = 0.949 mm, and θ = 60°. CFD validation confirmed that the optimized design reduced T_max from 307.639 K to 306.653 K, corresponding to a temperature drop of 0.986 K, while ΔT_max decreased from 8.752 K to 7.887 K, representing a reduction of 9.88%. Although the reduction in T_max is modest, the improvement in temperature uniformity is meaningful, which benefits cell consistency and long-term reliability. These results demonstrate that geometric optimization of cooling channels can provide an effective and energy-efficient approach to improving thermal uniformity in lithium-ion battery systems. Full article

Background and Objectives: Outcomes after trauma are traditionally attributed to injury severity and acute physiologic derangement. However, host vulnerability at presentation—reflecting underlying physiologic and nutritional status—may also be associated with bleeding severity and transfusion requirements following acute injury. Whether such vulnerability contributes additional risk information beyond established factors remains incompletely understood. Materials and Methods: We conducted a retrospective cohort study of adult trauma patients using a single-center trauma registry. Host vulnerability was assessed using a composite score (CE; range 0–3) based on admission hypoalbuminemia (<3.5 g/dL), anemia (hemoglobin < 11 g/dL), and reduced renal function (estimated glomerular filtration rate < 60 mL/min/1.73 m²). Primary outcomes were any blood transfusion and massive transfusion, defined as transfusion of ≥10 units of packed red blood cells within 24 h of admission. Associations between CE score and transfusion outcomes were evaluated using univariable and multivariable logistic regression models adjusted for age, Injury Severity Score (ISS), admission lactate level, and systolic blood pressure (SBP). Results: Among 4105 trauma patients, transfusion requirements increased progressively with higher CE scores. Rates of any transfusion rose from 21.7% in patients with CE 0 to 78.6% in those with CE 3, while massive transfusion increased from 1.9% to 23.1% across the same categories. In multivariable analyses, each 1-point increase in CE score was independently associated with higher odds of any transfusion (adjusted odds ratio [aOR] 3.21, 95% confidence interval [CI] 2.80–3.68) and massive transfusion (aOR 1.73, 95% CI 1.45–2.07). Conclusions: A composite score reflecting host vulnerability at presentation was associated with bleeding severity and transfusion requirements after trauma, beyond injury severity and acute physiologic factors. These findings suggest that simple laboratory-based markers may provide additional information for early risk stratification of hemorrhagic outcomes after trauma. Full article

Developmental engineering (DE) is a bottom-up strategy for generating functional tissues from modular tissues (MTs), offering potential advantages over conventional top-down approaches. However, scalable MT production remains constrained by limited understanding of scaffold aggregation, oxygen transport, and hydrodynamic effects in bioreactors. This study integrates theoretical simulations with empirical correlations to analyze these factors and provide a systematic basis for MT production. Microcarrier aggregates were modelled to evaluate minimum oxygen concentration (C_min). Results indicate that larger microcarrier diameters (d_mc) are associated with increased C_min due to longer diffusion distances. Aggregate geometry and packing configuration, including hexagonal close packing and the “kissing number,” influenced oxygen distribution and may explain observed C_min plateaus. Hydrodynamic behaviour was assessed using the Zwietering correlation and Kolmogorov turbulence scaling. Denser microcarrier aggregates required higher minimum stirring speeds (N_min), while larger d_mc increased susceptibility to shear. Increased agitation intensity and more aggressive impeller designs reduced N_min but were associated with potential cell damages. Higher medium density (e.g., 20% FBS) reduced shear stress and energy dissipation. A unified framework integrating oxygen diffusion, aggregate geometry, microcarrier properties, and hydrodynamics is proposed to estimate oxygen limitation and cell damage, highlighting trade-offs relevant to MT production in DE. Full article

This study focused on elucidating the effects of chitosan (Ch), hyaluronic acid (HA), and titanium dioxide nanoparticles (nano-TiO₂) on the physicochemical characteristics of a model bacterial membrane (layer) composed of the phospholipid DPPG (1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt). The membrane was prepared on mica using the Langmuir–Blodgett (LB) technique from an aqueous subphase containing Ch, HA and/or TiO₂. Its surface properties were subsequently characterized by optical profilometry and surface free energy estimation. The nanoscale topography of the DPPG layer provided a biomimetic platform that reflects the organization of bacterial membranes, enabling a precise evaluation of how external agents, such as Ch, HA, and nano-TiO₂, modify the surface’s structural and energetic properties. The results showed that the LB films exhibit mildly heterogeneous topography, which can be attributed to lipid domains with distinct molecular packing densities. Depending on the type of biopolymer employed with TiO₂, distinct topographic architectures of the DPPG monolayers were obtained. Furthermore, the presence of nano-TiO₂ was clearly manifested as a topographic irregularity, while the analysis of hydrophilic–hydrophobic properties revealed a structurally perturbed lipid film. The results provide detailed insight into how these specific molecules (Ch, HA, nano-TiO₂) interact at the molecular level with model bacterial membranes, offering a comprehensive picture of cell–microenvironment interactions. Full article

Life evolved under a persistent 1 g field that is continuous, ubiquitous, and directionally structured. Here, we synthesize evidence across evolutionary biology, mechanobiology, and genome architecture to propose gravity as a mechanical boundary condition that helped canalize the emergence of complex multicellularity. Order-of-magnitude considerations indicate that gravity-derived hydrostatic loads can fall within force/pressure regimes relevant to nuclear and chromatin mechanosensitivity when transmitted through adhesion–cytoskeleton–LINC–lamina coupling. Comparative genomic and imaging frameworks suggest that complex animals increasingly rely on volumetric genome organization (packing domains and higher-order 3D architectures) that supports durable transcriptional memory and stable differentiated cell identities. Integrating these concepts with altered-gravity experiments, we argue that microgravity and hypergravity perturb chromatin topology and region-level transcription in rapid, largely reversible patterns consistent with a mechanically defined 1 g reference state. We advance a boundary-condition thesis: gravity is not a sole driver but a stable reference that likely contributed to the evolvability and long-term robustness of mechanogenomic architectures required for high-dimensional differentiation and tissue homeostasis. Full article

Background/Objectives: Wolfram syndrome type 1 (WS1) is a rare, progressive, multisystem neurodegenerative disorder characterized by diabetes mellitus, optic atrophy, diabetes insipidus, and sensorineural hearing loss. As survival has improved, an increasing number of affected women are reaching reproductive age. However, evidence on pregnancy and peripartum management in WS1 remains scarce, and practical guidance is limited. This case report describes the multidisciplinary management of pregnancy and delivery in a woman with genetically confirmed WS1 and highlights key considerations for peripartum care. Case Presentation: A woman with genetically confirmed WS1 and long-standing multisystem involvement, including diabetes mellitus, diabetes insipidus, neurogenic bladder requiring frequent self-catheterization, progressive neurologic manifestations, and severe sensory impairment, achieved pregnancy through assisted reproduction with oocyte donation and was closely monitored by a multidisciplinary team. Due to persistent breech presentation, a planned external cephalic version was performed at 37 + 5 weeks’ gestation with immediate availability for cesarean delivery. After unsuccessful attempts, cesarean delivery was performed under combined spinal–epidural anesthesia. Peripartum management focused on strict glycemic control, careful monitoring of fluid balance and urine output, neuraxial anesthesia with proactive hemodynamic management, precautions related to the cochlear implant, and tailored communication strategies. Postpartum recovery was favorable, although anemia on postoperative day 1 required transfusion of one unit of packed red blood cells and intravenous iron therapy. Discussion and Conclusions: Pregnancy in WS1 represents a high-risk clinical scenario because of the coexistence of endocrine, urologic, and neurologic comorbidities, while published evidence on peripartum management remains limited. This case supports an individualized, multidisciplinary approach to obstetric and anesthetic planning and the use of a practical framework to optimize peripartum management and enhance maternal–fetal safety in this rare condition. Full article

Immersion cooling has been widely investigated in battery thermal management due to its high cooling efficiency; however, the influence of coolant properties on the thermal behavior and temperature uniformity of large-capacity energy storage battery modules remains unclear. In this study, a three-dimensional numerical model is developed to investigate the thermal performance of an immersion-cooled battery module consisting of 52 prismatic cells. The cooling performance of silicone oil (SO), synthetic hydrocarbon (SH), and two synthetic esters (SE) with different viscosities is systematically compared under various discharge rates and volumetric flow rates. The battery thermal model was validated through single-cell experiments under natural air convection conditions. The research results indicate that at a 0.5C discharge rate, the 30 cSt SE achieves a reduction in maximum battery pack temperature of 6.3% and 7.0% compared to SO and SH, respectively. Furthermore, the maximum temperature difference is significantly reduced by 22.9% and 25.4% under the same conditions. Due to differences in the inherent properties and flow heat transfer characteristics of the coolant, at a volumetric flow rate of 12 L/min, the 30 cSt SE resulted in a 15.8% reduction in module temperature difference compared to the 20 cSt SE. To further evaluate the internal thermal balance of the battery module, two thermal uniformity indicators were introduced to quantify the consistency of the highest temperature of individual cells and the internal temperature difference. Considering both the temperature performance and thermal uniformity at the module level, from a heat dissipation performance perspective, the 30 cSt SE demonstrates significant potential for thermal management of large-scale prismatic battery packs. Full article

Background/Objectives: An 8-kg, 16-month-old child was brought to the emergency department of a regional community hospital with shallow respirations. Due to her pallor and the diluted appearance of the first blood sample, the emergency physician suspected sepsis associated with severe anemia. Her first laboratory results revealed a hemoglobin of 1.7 g/dL. Subsequent laboratory data revealed positive fibrin split products and hypofibrinogenemia with reticulocytosis. Because this regional community hospital did not have a pediatric intensivist, the emergency physician instead consulted a neonatal intensivist for guidance. Methods: A femoral intraosseous line was placed to allow aggressive massive transfusion. After consultation with the neonatal intensivist, packed red blood cells were transfused at a rate of 30 mL/kg/h. After transfusion, the patient became agitated and required repeated paralytic, sedative, and analgesic boluses of succinylcholine, ketamine, midazolam, dexmedetomidine, and fentanyl, with fentanyl and dexmedetomidine drips. The patient arrived at a tertiary care center 13 h after admission. Results: At the tertiary care center, the patient was weaned off the drips and was theorized to have secondary autoimmune hemolytic anemia due to sepsis after positive direct and indirect Coombs test. She was treated with a course of antibiotics, including cefepime and vancomycin, without steroids or immunotherapy. Five months later, her hemoglobin had returned to 12.1 g/dL, and she tested negative on direct and indirect Coombs test. Conclusions: This case highlights the importance of collaboration between and within departments to successfully manage pediatric hemostatic resuscitation. Full article

Additive engineering has become a critical strategy for optimizing the morphology and performance of bulk heterojunction (BHJ) organic solar cells (OSCs), while volatile solid additives have been widely employed to control nanoscale phase separation during film formation. Concerns regarding reproducibility, residual solvent effects, and long-term stability have stimulated increasing interest in non-volatile solid additives. In recent years, solid additive engineering has emerged as a promising approach for modulating molecular packing, regulating phase separation, enhancing charge transport, and improving device stability. However, a systematic analysis of its material design principles and performance impact remains limited. This review summarizes recent progress in solid additive engineering for OSCs, categorizing reported additives into non-volatile, volatile and nanomaterials. The effects of these additives on key photovoltaic parameters, including open-circuit voltage (Voc), short-circuit current density (Jsc), fill factor (FF), and power conversion efficiency (PCE), are comparatively analyzed based on the reported data. Particular emphasis is placed on morphology and structural performance relationships and stability enhancement mechanisms. Finally, current challenges, including the lack of universal molecular design rules and limited mechanistic understanding of additive host interactions, are discussed, and future research directions are proposed. This review aims to provide a comprehensive perspective on the material-level role of solid additives and to guide the rational design of next-generation high-performance and stable organic solar cells. Full article

Verbascum hasbenlii Aytaç & H. Duman is a narrowly distributed endemic species native to Çanakkale, Türkiye. This study aimed to establish and optimize callus induction and cell suspension culture systems for V. hasbenlii. Leaf explants obtained from 10-week-old seed-derived in vitro plants were cultured on six Murashige and Skoog (MS) media containing different combinations of α-naphthaleneacetic acid (NAA; 0.5 or 1.0 mg/L) and 6-Benzylaminopurine (BAP; 0.5, 1, 2 or 3 mg/L). After four weeks, callus induction was achieved in all treatments (96–100%), although significant differences were observed in explant browning, callus biomass, diameter, and morphology. The medium supplemented with 0.5 mg/L BAP + 0.5 mg/L NAA produced the highest callus biomass (1.245 g) and diameter (5.06 mm), while maintaining low explant browning and a compact-friable texture suitable for suspension culture establishment. Cell suspension cultures exhibited a typical growth pattern with lag, exponential, and stationary phases. On day 9, cultures showed increased growth parameters, including packed cell volume (PCV: 7.50%), fresh weight (FW: 0.0580 g), and dry weight (DW: 0.0052 g), with relatively high cell viability (80.72%). Biomass accumulation reached maximum levels between days 18–21, while cell viability decreased to 66.82%. These findings provide an optimized in vitro culture system for future studies on secondary metabolite production in V. hasbenlii. Full article