Search Results (83)

Memory is a fundamental neurological function, essential for animal survival. Over the course of vertebrate evolution, elaborations in the forebrain telencephalon create new memory mechanisms, meaning basal vertebrates such as amphibians must have a less sophisticated system of memory acquisition, storage, and retrieval than the well-known hippocampal-based circuitry of mammals. Personality also appears to be a fundamental vertebrate trait and is generally defined as consistent individual behavior over time and across life history stages. In anuran amphibians (frogs), personality studies generally ask whether adult frogs retain the personality of their tadpole stage or whether personality shifts with metamorphosis, an idea behavioral ecologists term adaptive decoupling. Using a multidisciplinary perspective and recognizing there are ~7843 species of frogs, each with some molecular, morphological, physiological, or behavioral feature that makes it unique, we review, clarify, and provide perspective on what we collectively know about memory and personality and their mechanisms in anuran amphibians. We propose four working hypotheses: (1) as tadpoles grow, new telencephalic neurons become integrated into functional networks, producing behaviors that become more sophisticated with age; (2) since carnivores tend to be more bold/aggressive than herbivores, carnivorous anuran adults will be more aggressive than herbivorous tadpoles; (3) each amphibian species, and perhaps life history stage, will have a set point on the Shy–Bold Continuum; and (4) around this set point there will be a range of individual responses. We also suggest that several factors are slowing our understanding of the variety and depth of memory and personality possibilities in anurans. These include the scala natura approach to comparative studies (i.e., the idea that one frog represents all frogs); the assumption that amphibians are no more than simple reflex machines; that study species tend to be chosen more for convenience than taxonomic representation; and that studies are designed to prove or disprove a construct. This latter factor is a particular hindrance because what we are really seeking as scientists is not the confirmation or refutation of ideas, but rather what those ideas are intended to produce, which is understanding. Full article

Madagascar harbors a rich marine biodiversity, yet detailed knowledge of its fish species remains limited. Of the 1689 species listed in 2018, only 22% had accessible cytochrome oxidase I (COI) sequences in public databases. In response to growing pressure on fishery resources, this study aims to strengthen biodiversity monitoring tools. Its objectives were to enrich the COI database for Malagasy marine fishes, create the first 12S reference library, and evaluate the taxonomic resolution of different 12S metabarcodes for eDNA analysis, namely MiFish, Teleo1, AcMDB, Ac12S, and 12SF1/R1. An integrated approach combining morphological, molecular, and phylogenetic analyses was applied for specimen identification of fish captured using various types of fishing gear in Toliara and Ranobe Bays from 2018 to 2023. The Malagasy COI database now includes 2146 sequences grouped into 502 Barcode Index Numbers (BINs) from 82 families, with 14 BINs newly added to BOLD (The Barcode of Life Data Systems), and 133 cryptic species. The 12S library comprises 524 sequences representing 446 species from 78 families. Together, the genetic datasets cover 514 species from 84 families, with the most diverse being Labridae, Apogonidae, Gobiidae, Pomacentridae, and Carangidae. However, the two markers show variable taxonomic resolution: 67 species belonging to 35 families were represented solely in the COI dataset, while 10 species from nine families were identified exclusively in the 12S dataset. For 319 species with complete 12S gene sequences associated with COI BINs (Barcode Index Numbers), 12S primer sets were used to evaluate the taxonomic resolution of five 12S metabarcodes. The MiFish marker proved to be the most effective, with an optimal similarity threshold of 98.5%. This study represents a major step forward in documenting and monitoring Madagascar’s marine biodiversity and provides a valuable genetic reference for future environmental DNA (eDNA) applications. Full article

The off-the-track Thoroughbred’s (OTTTB’s) suitability for equestrian retraining and rehoming should always be subject to behavioral considerations. Certain attributes may be advantageous to a horse’s racing performance but unfavorable to their prospects off the track. It is important to gain a non-biased understanding of how Thoroughbreds (TBs) in equestrian disciplines compare with other horses behaviorally, to minimize risks of poor welfare and safety outcomes. The current study used owner-reported information (n = 1633) from the Equine Behavior Assessment and Research Questionnaire (E-BARQ) global database to compare the behaviors of OTTTBs with those of other ridden horses. Boldness, compliance, rideability, trainability, and responsiveness to acceleration and deceleration signals were evaluated in the context of 27 E-BARQ items, as determined by exploratory factor analysis (EFA). In this study, OTTTBs demonstrated more boldness (t = 3.793; p < 0.001) and lower compliance and responsiveness to deceleration signals (t = 3.448; p < 0.001) than non-OTTTBs. Trainability, rideability, and responsiveness to acceleration signals did not differ significantly between OTTTBs and non-OTTTBs. These findings provide direction for future research into causal factors and improvement opportunities regarding the training and management of Thoroughbreds, on- and off-the-track. Full article

Microalgae-based bioremediation is increasingly recognized as a sustainable, efficient, and straightforward technology. Despite this growing interest, the potential of Parachlorella hussii for metal biosorption remains underexplored. This study is the first report evaluating the metal biosorption activity in Parachlorella hussii ACOI 1508 (N9), highlighting the impact of the culture age on the monosaccharide composition and its correlation to the metal binding capacity. The capsular strain (N9) was isolated from the hypersaline ecosystem—Lake Chott Aïn El-Beida—in southeastern Algeria. Cultivated in Bold’s Basal medium, the strain produced 0.807 ± 0.059 g L⁻¹ of RPSs and 1.975 ± 0.120 g L⁻¹ of CPSs. Biochemical analysis of the extracts revealed a high total sugar content (% w/w) that ranged from 62.98 ± 4.87% to 95.60 ± 87% and a low protein content (% w/w) that ranged from 0.49 ± 0.08% to 1.35 ± 0.69%, with RPS-D7 and RPS-D14 having high molecular weight (≥2 MDa). HPLC-based monosaccharide characterization demonstrated compositional differences between the exponential and stationary phases, with rhamnose dominating (~55%) in RPS-D14 and with the presence of uronic acids comprising 7–11.3%. Metal removal efficiency was evaluated using the whole biomass in two growth phases. Copper uptake exhibited the highest capacity, reaching 18.55 ± 0.61 mg Cu g⁻¹ DW at D14, followed by zinc removal with 6.52 ± 0.61 mg Zn g⁻¹ DW. Interestingly, removal efficiencies increased to about twofold during the stationary phase, reaching 51.15 ± 1.14% for Cu, 51.08 ± 3.35% for Zn, and 36.55 ± 3.09% for Ni. The positive results obtained for copper/zinc removal highlight the biosorption potential of P. hussii, and notably, we found that the metal removal capacity significantly improved with culture age—a parameter that has been poorly investigated in prior studies. Furthermore, we observed a growth phase-dependent modulation in monosaccharide composition, which correlated with enhanced functional properties of the excreted biomolecules involved in biosorption. This metabolic adjustment suggests an adaptive response that may contribute to the species’ effectiveness in heavy metal uptake, underscoring its novelty and biotechnological relevance. Full article

The increasing incidence of osteoporotic fractures determines ongoing research on new methods and strategies for improving the difficult healing process of this type of fracture. Osteoporotic patients suffer from the intense side effects of accustomed drug treatment and its systemic distribution in the body. To overcome these drawbacks, besides searching for new drugs, 3D-printed scaffolds and drug delivery systems have started to be increasingly seen as the main strategy employed against osteoporosis. Three-dimensionally printed scaffolds can be tailored in intricate designs and make use of nanoscale topographical and biochemical cues able to enhance bone tissue regeneration. Research regarding drug delivery systems is exploring bold new ways of targeting bone tissue, making use of designs involving nanoparticles and intricate encapsulation and support methods. The local administration of treatment with the help of a scaffold-based drug delivery system looks like the best option through its use of the advantages of both structures. Biomimetic systems are considered the future norm in the field, while stimuli-responsiveness opens the door for the next level of efficiency, patient compliance, and a drastic reduction in side effects. The successful approval of these products still requires numerous challenges throughout the development and regulatory processes, but the interest and effort in this direction are high. This review explored various strategies for managing osteoporosis, emphasizing the use of scaffolds for targeted drug delivery to bone tissue. Instead of covering the whole subject, we focused on the most important aspects, with the intention to provide an up-to-date and useful introduction to the management of osteoporosis. Full article

Understanding inter-species differences in behavioral lateralization and exploration patterns is crucial for advancing the study of animal behavior. In this study, we standardized the experimental procedure to minimize methodological variability and examined the behavioral responses of eight fish species (Girardinus falcatus, Poecilia reticulata, Paracheidon axelrodi, Kriptoterus bichirris, Hyphessobrycon megalopterus, Danio rerio, Corydoras aeneus, and Xenopoecilius sarasinorum) in a novel, circular environment. We focused on boldness-related measures (latency to explore and freezing time) and motor activity (circular vs. linear swimming). Significant inter-species differences were observed in the boldness measures. Fish also showed a preference for circular swimming over linear swimming. However, no lateralization bias (clockwise vs. counterclockwise) was detected in any species. These findings may establish a baseline for future research on the spontaneous behaviors of eight teleost species and offer valuable insights for the design of future behavioral studies focusing on these species. Full article

Background/Objectives: The insula is a deep, functionally heterogeneous region involved in various pathological conditions. Repetitive transcranial magnetic stimulation (rTMS) has emerged as a promising therapeutic avenue for neuromodulation, yet very few studies have directly investigated its effects on insular activity. Moreover, empirical evidence of target engagement of this region remains scarce. This study aimed to stimulate the insula with rTMS and assess blood oxygen level-dependent (BOLD) signal modulation using concurrent functional magnetic resonance imaging (fMRI). Methods: Ten participants were recruited, six of whom underwent a single session of 5 Hz high-frequency rTMS over the right insular cortex inside the MRI scanner. Stimulation was delivered using a compatible MRI-B91 TMS coil. Stimulation consisted of 10 trains of 10 s each, with a 50 s interval between trains. Frameless stereotactic neuronavigation ensured precise targeting. Paired t-tests were used to compare the mean BOLD signal obtained between stimulation trains with resting-state fMRI acquired before the rTMS stimulation session. A significant cluster threshold of q < 0.01 (False Discovery Rate; FDR) with a minimum cluster size of 10 voxels was applied. Results: Concurrent rTMS-fMRI revealed the significant modulation of BOLD activity within insular subregions. Increased activity was observed in the anterior, middle, and middle-inferior insula, while decreased activity was identified in the ventral anterior and posterior insula. Additionally, two participants reported transient dysgeusia following stimulation, which provides further evidence of insular modulation. Conclusions: These findings provide preliminary evidence that rTMS can modulate distinct subregions of the insular cortex. The combination of region-specific BOLD responses and stimulation-induced dysgeusia supports the feasibility of using rTMS to modulate insular activity. Full article

The issue of environmental protection and nature conservation has gained global importance, and its solution requires not only scientific and technological efforts but also the education of an environmentally conscious and active young generation. Children’s literature serves as an effective means for this task. The article analyzes the eco-pedagogical potential of contemporary Ukrainian children’s literature through the prism of young eco-rebels. These characters inspire readers with their emotional power, eco-centric worldview, and bold resistance to environmental injustice. They contribute to the formation of ecological values in readers through emotional impact. Based on the ecocritical interpretation and typological comparison of Ghosts of Black Oak Wood by Bachynskyi and Taming of Kychera by Polyanko, we observe that the components of representation of the ecological topic are problematic eco-situation; behavior models, young eco-rebels’ actions and deeds; and eco-initiatives. The article further presents the results of ecocritical dialogues on environmental topics with 26 readers aged 14–15 (Ukraine). The methodology included interactive tools (e.g., Padlet) and surveys, which revealed that literary engagement promoted critical thinking, empathy, and personal eco-involvement. The findings confirm that children’s literature, when integrated with dialogic and participatory teaching methods, can serve as a powerful tool for shaping environmental literacy and civic responsibility in youth. Full article

BOL/DRNL/ESR2, an AP2/ERF transcription factor, regulates early organ development in Arabidopsis (Arabidopsis thaliana). Its loss of function causes flower organ defects, while its overexpression induces green callus formation in roots without the addition of hormones. Jasmonates, plant hormones known as major players in stress responses, also regulate some aspects of organ development (e.g., stamen development and plant and organ growth). Here, we studied the interaction between BOL and the JA pathway. We found that exogenous application of methyl jasmonate (MeJA) partially rescued the stamen phenotypes in bol-cr mutants, linking BOL and JA-mediated stamen development. Moreover, MeJA treatments in wild-type plants partially mimicked some bol-D mutant phenotypes like reduced rosette and root size, while JA inhibition restored wild-type leaf curvature, suggesting an alteration in JA homeostasis in the gain-of-function mutant. BOL overexpression caused increased JA levels, whereas bol loss-of-function plants had reduced levels. Furthermore, inducible BOL activity led to downregulation of a JA-responsive marker. Finally, JA biosynthesis inhibition affected BOL-induced root callus formation and led to an expansion of the BOL expression domain in roots. Our findings indicate that BOL modulates parts of the JA pathway and that feedback from the JA pathway appears to affect expression of the transcription factor. Full article

Background/Objectives: Fibromyalgia (FM) is a chronic pain condition that includes symptoms of hyperalgesia and has an unknown etiology. This study aimed to further investigate the underlying neural signaling mechanisms and their relation to observed blood oxygenation-level dependent (BOLD) signal increases at the onset of functional magnetic resonance imaging (fMRI) runs. Methods: The possible neural mechanisms were first explored by reviewing the current literature. The second component of this study involved a voxel-by-voxel analysis of BOLD responses in all regions of the brain. The fMRI data were obtained from a previous study of participants with and without fibromyalgia during fMRI runs involving either a noxious heat pain stimulus or no stimulus. Results: The literature review indicates that no single factor can explain the initial BOLD signal rise observed in FM but that there are likely multiple interacting influences. These include physiological dysregulation via mechanisms, such as oxidative stress, mitochondrial dysfunction, and cytokine activity, and may involve the sympathetic nervous system. The analysis of BOLD responses demonstrated that the initial BOLD rises occur specifically in gray matter regions and are largest in regions involved with pain processing, including the right insular cortex and periaqueductal gray region. Moreover, the BOLD rise is significantly larger in people with FM prior to the application of a noxious stimulus. Conclusions: The initial rise in BOLD response demonstrates heightened metabolic demand that is exaggerated in people with FM. It appears to be influenced by cognitive factors such as anticipation and may reflect neural dysregulation, possibly involving autonomic signaling. Full article

A recent study found evidence of symbolic gesture use in Japanese tits (Parus minor). The study reveals how these birds use wing-fluttering movements to transmit an “after you” directive to their partners, implying a degree of cognitive skill previously thought to be unique to humans and great apes. If confirmed, this research contradicts long-held notions about animal communication by proving that Japanese tits not only participate in intricate vocal communications, which can comprise phrases with specific grammatical rules, but also use body language as a form of engagement. Here, we evaluate this claim by inspecting the data and utilizing bootstrapping to expand the sample size. We find a large variation in how frequently the “after you” gesture is employed in different bootstrap samples, suggesting that while the gesture is a consistent behavior, its frequency can vary significantly. Moreover, the timing of the male’s response to the female’s gesture can fluctuate, though the response itself appears to be a stable phenomenon. Beyond evaluating the bootstrapping technique, we employ causal inference to examine whether wing fluttering by female Japanese tits leads to a quicker response by males, entering the nest more swiftly. The analysis supports this hypothesis, suggesting that wing fluttering functions as an effective communicative gesture in this species, influencing male nesting behavior. However, because the frequency of the “after you” gesture varied greatly, especially in light of potential cognitive and other biases influencing the study, this bold claim should be taken with caution. Full article

Pastoral supervision and spiritual direction are inherently personal in nature. They reveal a transformative life of spiritual and soul care demanding the formation of theological reflection, healing, guidance, and discernment. Whilst pastoral supervision serves to guide theological reflection on service and mission, spiritual direction facilitates the moments of awakening to the presence and narrative of God’s grace and work in the depths of the soul. Under the light of Catholic Social Teaching, the notions of solidarity and subsidiarity will be pressed into service to unveil a common spiritual–theological foundation animating the roots of both these ministries. To understand the common dynamics of pastoral supervision and spiritual direction, the article develops a spiritual theology to reveal that both ministries, however distinct in practice, share the same incarnational (creative) and redemptive (re-creative) qualities. In other words, they both share the same source, root, origin, or existence in God’s word and grace. This comes to light by learning to pronounce love in truth, a prophetical work of solidarity and subsidiarity, of friendship and sharing orienting integral human development to listen and respond to God’s movement. The actions of solidarity and subsidiarity are rooted in the beatitude of righteousness revealing the little goodness, a work of possibility and responsibility. Such actions serve to transform the time of ministry into spaces of blessing and mystery, of appreciating the sacredness and unknowability of the other with boldness, curiosity and gentleness. The article concludes by showing how both ministries of pastoral supervision and spiritual direction share the spiritual-pastoral appeal to form communities witnessing the little goodness of love in truth. Full article

Territorial crustaceans exhibit a complex set of personality traits and behavioral characteristics that are critical for their survival, growth, and overall welfare. This study aimed to systematically investigate the interplay between two essential personality traits—boldness and aggression—in Chinese mitten crabs (Eriocheir sinensis), during various developmental stages under conditions of crowding stress and food odor stimuli. Using behavioral assays, we found that adults displayed significantly higher levels of boldness and aggression compared to juveniles. Notably, bold adult crabs demonstrated lower aggression than their shy counterparts, revealing a significant negative correlation between boldness and aggression under crowding stress. Furthermore, juvenile and adult crabs exhibited negative correlations between the stability of their boldness and aggression with individual levels of boldness and aggression, respectively. The study also found that while juvenile boldness did not significantly correlate with body size, larger individuals displayed higher boldness under stress, contrasting with adult crabs, where boldness negatively correlated with body size, although aggression levels were unaffected by size; the stability of adult boldness demonstrated a positive correlation with body size under crowding stress. These findings elucidate the intricate dynamics of personality traits in territorial crustaceans, laying the groundwork for future research into their adaptive behavioral strategies in response to environmental cues like group stress and food odors. Full article

Personality has been reported to influence fish response to stress. This study aimed to assess whether shy and bold fish display different sensitivities to two environmental contaminants: caffeine (CAF) and cadmium (Cd). Thus, the sensitivity to Cd was compared based on lethal concentrations (LCs). The potential different response to CAF, known to alter the social behavior and locomotor activity of zebrafish, was studied using behavioral parameters. Overall, different LC values were found for each group: 48 h LC₅₀ values of 4.79 (shy fish) and 8.20 mg·L⁻¹ (bold fish); and 96 h LC₅₀ values of 3.79 (shy fish) and 9.79 mg·L⁻¹ (bold fish). In terms of response to CAF, a significant interaction between CAF and personality traits (bold and shy) was found in the locomotion activities (distance travelled, and medium and rapid movements), in the mirror test (frequency of contact and entries into the contact, approach, and distant zones), and in social tests (swimming distance in zones 2 and 3; time spent in zones 1, 2, and 3; and number of entries into zones 1 and 2). Shy fish exposed to 300 μg·L⁻¹ of CAF presented hypoactivity, reduced aggressive behavior, and reduced sociability. Conversely, CAF did not influence the behavior of bold fish. In general, shy fish were more sensitive to Cd and exhibited anxious behavior when exposed to CAF, which appears to be the factor responsible for changes in their social behavior. Our results highlight the importance of taking personality traits into account in future studies, as variations in behavioral responses between bold and shy individuals can mask the toxicological effects of different chemicals. Full article

The functional significance of RSNs is examined via simultaneous EEG-fMRI studies on the basis of the relation of RSNs with different frequency bands of EEG and EEG-based microstate analysis. In this study, we try to identify RSNs from microstates of cortical surface maps of the BOLD signal. In addition, the scale-free dynamics of these map sequences were also examined. The structural and resting state functional MRI images were acquired on a 3T scanner with three different fMRI acquisition protocols from seven subjects. Microstate segmentations from EEG, fMRI, and simulated data were evaluated. Wavelet-based fractal analysis was performed on map sequence time series and the Hurst exponent (H) was calculated. By using HRF-deconvolved fMRI time series, the effect of the HRF (hemodynamic response function) on fMRI-derived microstates was tested. The fMRI map sequence has a system with a memory system smaller than 16 s. When the HRF was deconvolved, the duration of the memory of the system was reduced to 4 s. On the other hand, the results of simulation data indicated that these systems are specific to the resting state BOLD signal. Similar to EEG microstates, fMRI also has microstates and both of them have scale-free dynamics. fMRI microstate dynamics have two different components, one is related to the HRF and the other is independent of the HRF. The significance of fMRI microstates and their relation with RSNs need to be further studied. Full article