Search Results (14)

This study investigated the potential of chicken byproduct hydrolysates (CBH) characterized by a mixture of low-molecular-weight peptides (<1.35 kDa) and larger peptides (<17.5 kDa) as a treatment for metabolic syndrome (MS), from a histological and histopathological point of view. This study aimed to evaluate the effects of CBH obtained using plant proteases (BP: B. pinguin, BK: B. karatas, BRO: bromelain) on the histological and histopathological analysis of the liver and kidney in an MS-induced murine model. Methods: Thirty adult male Wistar rats were randomly assigned to six groups (n = 5): (1) standard diet (STD); (2) MS with a hypercaloric diet (MS + HC); (3) CBH-BP (200 mg/kg of body weight); (4) CBH-BK (200 mg/kg of body weight); (5) CBH-BRO (200 mg/kg of body weight); (6) carnosine (CAR) 50 mg/kg of body weight. Liver and kidney samples were processed by conventional hematoxylin and eosin (H&E) histological techniques, Masson’s trichrome stain (MTS), and the periodic acid–Schiff (PAS) histochemical method. A scoring scale was used for the histopathological evaluation with scores ranging from 0 (normal tissue) to 4 (severe damage). Results: CBHs demonstrated a significant therapeutic effect (p < 0.05) on hepatic and renal morphological alterations induced by MS. Hepatic scores for lipid inclusions, vascular congestion, and cellular alteration were all reduced to below two. Similarly, renal scores for tubular degeneration, vascular congestion, and dilation of Bowman’s space were also decreased to less than two. The therapeutic efficacy of CBHs was comparable to that of the positive control, CAR (β-alanyl-L-histidine). Conclusions: CBH-BP, CBH-BK, and CBH-BRO treatments reduced morphological alterations observed in liver and kidney tissues, which is relevant since from a histological and histopathological point of view, it allows us to understand at the cellular and tissue level the effects that these treatments can have on a living organism, indicating a potential to improve organ health in people with MS. Full article

The toxicity of food additives is widely studied and concerns many consumers worldwide. Synthetic food colors are often considered an unnecessary risk to consumer health. Since the European Food Safety Authority’s (EFSA) re-evaluation between 2009 and 2014, the body of scientific literature on food colors has grown, and new evaluations are being published by the Joint FAO/WHO Expert Committee on Food Additives (JECFA). Therefore, this narrative review aims to review the toxicological data that have become available since 2014. The reviewed colors are Quinoline Yellow, Sunset Yellow, Azorubine, Amaranth, Ponceau 4R, Erythrosine, Allura Red, Patent Blue, Indigo Carmine, Brilliant Blue FCF, Green S, Brilliant Black, Brown HT, and Lithol Rubine BK. Tartrazine was not included in this paper; the overwhelming amount of recent data on Tartrazine toxicity requires more space than this review can provide. The issues regarding the toxicity of synthetic food colors and real population exposures are being regularly examined and reviewed by relevant authorities, such as the EFSA and JECFA. The current ADI limits set by the authorities are mostly in agreement, and they seem safe. However, the EFSA and JECFA assessments of some of the colors are more than a decade old, and new evidence will soon be required. Full article

Nanoslits have various applications, including localized surface plasmon resonance (LSPR)-based nanodevices, optical biosensors, superfocusing, high-efficiency refractive index sensors and chip-based protein detection. In this study, the effect of substrates on the optical properties of gold nanoslits placed in free space is discussed; for this purpose, glass BK7 and Al${}_2$O${}_3$ are used as substrates and the wavelength of incident light is supposed to be 650 nm. The optical properties, power flow and electric field enhancement for gold nanoslits are investigated by using the finite element method (FEM) in COMSOL Multiphysics software. The effect of polarization of an incident electromagnetic wave as it propagates from a gold nanoslit is also analyzed. As special case, the effect of glass and alumina substrate on magnetic field, power flow and electric field enhancement is discussed. The goal of this research is to investigate the phenomenon of power flow and electric field enhancement. The study of power flow in gold nanoslits provides valuable insights into the behavior of light at the nanoscale and offers opportunities for developing novel applications in the field of nanophotonics and plasmonics. The consequences of this study show the significance of gold nanoslits as optical nanosensors. Full article

Future space missions to Mars will depend on the development of bioregenerative life support systems. Mars regolith contains most of the nutrients needed for plant growth, but not organic matter (OM). Although Mars simulants have been deeply characterized and tested as growing media, no data are available about their possible modification occurring during terraforming, including the interaction of exogeneous OM with iron (Fe) oxides, particularly abundant in Mars regolith. The aim of this study was to investigate the mineral transformation and the OM evolution occurring in the early stages of the terraforming process. Potato was grown for 99 days on Mojave Mars Simulant MMS-1, alone (R100) and mixed with a compost 70:30 v:v (R70C30), and on a fluvial sand, alone (S100) and mixed with compost (S70C30), for comparison. Bulk (BK) and potato tubero/rhizo-sphere (RH) soils were fractionated to obtain particulate OM (POM) and mineral-associated OM (MAOM). Bulk samples and corresponding fractions were characterized for total nitrogen and organic carbon (C) and analyzed by Fe K-edge X-ray absorption near-edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) spectroscopy. Organic C increased by 10 and 25 times in S70C30 and R70C30, respectively, compared to S100 and R100. Most of the organic C accumulated in the POM fraction of both growing substrates, while its content in the MAOM was 3 times higher in R70C30 than in S70C30. No significant differences between BK and RH were found. Finally, ferrihydrite mediated exogenous OM stabilization in regolith-based substrates, while Fe(III)-OM complexes were detected exclusively in sand-based growing media. Understanding mechanisms and testing potential sustainable practices for creating Mars regolith similar to terrestrial soil will be fundamental to sustain food crop production on Mars. Full article

Glioblastoma (GBM) is the most malignant form of primary brain tumor. It is characterized by the presence of highly invasive cancer cells infiltrating the brain by hijacking neuronal mechanisms and interacting with non-neuronal cell types, such as astrocytes and endothelial cells. To enter the interstitial space of the brain parenchyma, GBM cells significantly shrink their volume and extend the invadopodia and lamellipodia by modulating their membrane conductance repertoire. However, the changes in the compartment-specific ionic dynamics involved in this process are still not fully understood. Here, using noninvasive perforated patch-clamp and live imaging approaches on various GBM cell lines during a wound-healing assay, we demonstrate that the sodium-calcium exchanger (NCX) is highly expressed in the lamellipodia compartment, is functionally active during GBM cell migration, and correlates with the overexpression of large conductance K+ channel (BK) potassium channels. Furthermore, a NCX blockade impairs lamellipodia formation and maintenance, as well as GBM cell migration. In conclusion, the functional expression of the NCX in the lamellipodia of GBM cells at the migrating front is a conditio sine qua non for the invasion strategy of these malignant cells and thus represents a potential target for brain tumor treatment. Full article

We determine the multipliers $M(X,Y)$ where X, $Y\in \{w_0,w,w_{\infty }\}$, and we apply these results to the solvability of both the (SSIE) of the form $F\subset \mathcal{E}+w_x$, where $F\in \{w_0,w,w_{\infty }\}$, and the (SSIE) $w\subset \mathcal{E}+W_x^0$ and $w\subset \mathcal{E}+W_x$. Full article

National Aeronautics and Space Administration’s (NASA) spacecraft assembly facilities are monitored for the presence of any bacteria or fungi that might conceivably survive a transfer to an extraterrestrial environment. Fungi present a broad and diverse range of phenotypic and functional traits to adapt to extreme conditions, hence the detection of fungi and subsequent eradication of them are needed to prevent forward contamination for future NASA missions. During the construction and assembly for the Mars 2020 mission, three fungal strains with unique morphological and phylogenetic properties were isolated from spacecraft assembly facilities. The reconstruction of phylogenetic trees based on several gene loci (ITS, LSU, SSU, RPB, TUB, TEF1) using multi-locus sequence typing (MLST) and whole genome sequencing (WGS) analyses supported the hypothesis that these were novel species. Here we report the genus or species-level classification of these three novel strains via a polyphasic approach using phylogenetic analysis, colony and cell morphology, and comparative analysis of WGS. The strain FJI-L9-BK-P1 isolated from the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) exhibited a putative phylogenetic relationship with the strain Aaosphaeria arxii CBS175.79 but showed distinct morphology and microscopic features. Another JPL-SAF strain, FJII-L3-CM-DR1, was phylogenetically distinct from members of the family Trichomeriaceae and exhibited morphologically different features from the genera Lithohypha and Strelitziana. The strain FKI-L1-BK-DR1 isolated from the Kennedy Space Center facility was identified as a member of Dothideomycetes incertae sedis and is closely related to the family Kirschsteiniotheliaceae according to a phylogenetic analysis. The polyphasic taxonomic approach supported the recommendation for establishing two novel genera and one novel species. The names Aaosphaeria pasadenensis (FJI-L9-BK-P1 = NRRL 64424 = DSM 114621), Pasadenomyces melaninifex (FJII-L3-CM-DR1 = NRRL 64433 = DSM 114623), and Floridaphiala radiotolerans (FKI-L1-BK-DR1 = NRRL 64434 = DSM 114624) are proposed as type species. Furthermore, resistance to ultraviolet-C and presence of specific biosynthetic gene cluster(s) coding for metabolically active compounds are unique to these strains. Full article

For the reconstruction of 3D MRI data that are accelerated along the two phase-encoding directions, the 2D-generalized autocalibrating partially parallel acquisitions (GRAPPA) algorithm can be used to estimate the missing data in the k-space. We propose a new boomerang-shaped kernel based on theoretic and systemic analyses of the shape and dimensions of the kernel. The reconstruction efficiency of the 2D-GRAPPA algorithm with the proposed boomerang-shaped kernel (i.e., boomerang kernel (BK)-2D-GRAPPA) was compared with other 2D-GRAPPA algorithms that utilize different types of kernels (i.e., EX-2D-GRAPPA and SK-2D-GRAPPA) based on computer simulation, phantom and in vivo experiments. The proposed method was validated for different sets of ACS lines with acceleration factors from four to eight and various sizes of the kernels. A quantitative analysis was also performed by comparing the normalized root mean squared error (nRMSE) in the images and the undersampled edges. Computer simulation, in vivo and phantom experiments, and the quantitative analysis, showed that the proposed method could reduce aliasing artifacts without reducing the SNRs of the reconstructed images. Full article

We present a survey of the theory of measures of noncompactness and discuss some fixed point theorems of Darbo’s type. We apply the technique of measures of noncompactness to the characterization of classes of compact operators between certain sequence spaces, in solving infinite systems of integral equations in some sequence spaces. We also present some recent results related to the existence of best proximity points (pairs) for some classes of cyclic and noncyclic condensing operators in Banach spaces equipped with a suitable measure of noncompactness. Finally, we discuss the existence of an optimal solution for systems of integro–differentials. Full article

Botryosphaeria spp. are important phytopathogenic fungi that infect a wide range of woody plants, resulting in big losses worldwide each year. However, their pathogenetic mechanisms and the related virulence factors are rarely addressed. In this study, seven lignin peroxidase (LiP) paralogs were detected in Botryosphaeria kuwatsukai, named BkLiP1 to BkLiP7, respectively, while only BkLiP1 was identified as responsible for the vegetative growth and virulence of B. kuwatsukai as assessed in combination with knock-out, complementation, and overexpression approaches. Moreover, BkLiP1, with the aid of a signal peptide (SP), is translocated onto the cell wall of B. kuwatsukai and secreted into the apoplast space of plant cells as expressed in the leaves of Nicotiana benthamiana, which can behave as a microbe-associated molecular pattern (MAMP) to trigger the defense response of plants, including cell death, reactive oxygen species (ROS) burst, callose deposition, and immunity-related genes up-regulated. It supports the conclusion that BkLiP1 plays an important role in the virulence and vegetative growth of B. kuwatsukai and alternatively behaves as an MAMP to induce plant cell death used for the fungal version, which contributes to a better understanding of the pathogenetic mechanism of Botryosphaeria fungi. Full article

A new biomechanism, Bioactive Kinetic Screw (BKS) for screws and bone implants created by the first author, is presented using a bone dental implant screw, in which the bone particles, blood, cells, and protein molecules removed during bone drilling are used as a homogeneous autogenous transplant in the same implant site, aiming to obtain primary and secondary bone stability, simplifying the surgical procedure, and improving the healing process. The new BKS is based on complex geometry. In this work, we describe the growth factor (GF) delivery properties and the in situ optimization of the use of the GF in the fixation of bone screws through a dental implant. To describe the drilling process, an explicit dynamic numerical model was created, where the results show a significant impact of the drilling process on the bone material. The simulation demonstrates that the space occupied by the screw causes stress and deformation in the bone during the perforation and removal of the particulate bone, resulting in the accumulation of material removed within the implant screw, filling the limit hole of the drill grooves present on the new BKS. Full article

Given any sequence $z={\left(z_n\right)}_{n\ge 1}$ of positive real numbers and any set E of complex sequences, we write $E_z$ for the set of all sequences $y={\left(y_n\right)}_{n\ge 1}$ such that $y/z={\left(y_n,/,z_n\right)}_{n\ge 1}\in E$; in particular, ${\mathbf{s}}_z^0$ denotes the set of all sequences y such that $y/z$ tends to zero. Here, we consider the infinite tridiagonal matrix $\widetilde{B\left(r,,,s,,,t\right)}$, obtained from the triangle $B\left(r,,,s,,,t\right)$, by deleting its first row. Then we determine the sets of all positive sequences $a={\left(a_n\right)}_{n\ge 1}$ such that ${\left(E_a\right)}_{\widetilde{B\left(r,,,s,,,t\right)}}\subset E_a$, where $E={\ell }_{\infty }$, $c_0$, or c. These results extend some recent results. Full article

Given any sequence $a={\left(a_n\right)}_{n\ge 1}$ of positive real numbers and any set E of complex sequences, we can use $E_a$ to represent the set of all sequences $y={\left(y_n\right)}_{n\ge 1}$ such that $y/a={(y_n/a_n)}_{n\ge 1}\in E$. In this paper, we use the spaces $w_{\infty }$, $w_0$ and w of strongly bounded, summable to zero and summable sequences, which are the sets of all sequences y such that ${\left(n^{-1}{\sum }_{k=1}^n\left|y_k\right|\right)}_n$ is bounded and tends to zero, and such that $y-le\in w_0$, for some scalarl. These sets were used in the statistical convergence. Then we deal with the solvability of each of the SSIE $F_{\Delta }\subset Ɛ+F_x^{\prime }$, where $Ɛ$ is a linear space of sequences, $F=c_0$, c, ${\ell }_{\infty }$, $w_0$, w or $w_{\infty }$, and $F^{\prime }=$$c_0$, c or ${\ell }_{\infty }$. For instance, the solvability of the SSIE $w_{\Delta }\subset w_0+s_x^{\left(c\right)}$ relies on determining the set of all sequences $x={\left(x_n\right)}_{n\ge 1}\in U^{+}$ that satisfy the following statement. For every sequence y that satisfies the condition ${lim}_{n\to \infty }{n}^{-1}{\sum }_{k=1}^n\left|y_k-y_{k-1}-l\right|=0$, there are two sequences u and v, with $y=u+v$ such that ${lim}_{n\to \infty }{n}^{-1}{\sum }_{k=1}^n\left|u_k\right|=0$ and ${lim}_{n\to \infty }\left(v_n/x_n\right)=L$ for some scalars l and L. Full article

RSS-based indoor positioning is a consolidated research field for which several techniques have been proposed. Among them, Bluetooth Low Energy (BLE) beacons are a popular option for practical applications. This paper presents a new BLE RSS database that was created to aid in the development of new BLE RSS-based positioning methods and to encourage their reproducibility and comparability. The measurements were collected in two university zones: an area among bookshelves in a library and an area of an office space. Each zone had its own batch of deployed iBKS 105 beacons, configured to broadcast advertisements every 200 ms. The collection in the library zone was performed using three Android smartphones of different brands and models, with beacons broadcasting at −12 dBm transmission power, while in the other zone the collection was performed using of one those smartphones with beacons configured to advertise at the −4 dBm, −12 dBm and −20 dBm transmission powers. Supporting materials and scripts are provided along with the database, which annotate the BLE readings, provide details on the collection, the environment, and the BLE beacon deployments, ease the database usage, and introduce the reader to BLE RSS-based positioning and its challenges. The BLE RSS database and its supporting materials are available at the Zenodo repository under the open-source MIT license. Full article