Search Results (8)

Inoculation with Rhizobium can improve not only host yield and quality but also host adaptability to stress. The match between inoculated strains and hosts and resistance to stress are the key factors determining the inoculation effect. Four types of Desmodium, Pleurolobus gangeticus, Puhuaea sequax, Grona heterocarpos, and Polhillides velutina, were used to capture Rhizobium strains in soil, and the phylogenetic classification and salt and acid—alkaline resistances of the strains were determined through isolation, identification, and different salt and pH treatments. The phylogenetic classification of the 62 rhizobial strains was determined by 16S rDNA analysis. After comparison, the strains were grouped into 10 groups with known strains of rhizobia, including 14 strains of fast-growing rhizobia grouped with Rhizobium tropici and Rhizobium oryzicola and 48 strains of slow-growing rhizobia. The salt tolerance and acid—base adaptability of 52 strains were qualitatively and quantitatively determined in this study, and salt stress was found to have varying effects on the growth of different strains. Some strains grew normally on media with NaCl concentrations of 0.4 and 0.35 mol/L and had strong salt tolerance. The plants grew normally on media with pH 5–9. This study provides theoretical support for the classification of Desmodium and provides efficient Rhizobium strains for the cultivation and application of Desmodium. Full article

The endemic and critically endangered gharial, Gavialis gangeticus, experienced a severe population decline in its range. However, conservation efforts, notably through the implementation of “Project Crocodile” in India, have led to a significant recovery of its population. The present study employs an ensemble Species Distribution Model (SDM) to delineate suitable habitats for G. gangeticus under current and future climatic scenarios to understand the impact of climate change. The model estimates that 46.85% of the area of occupancy is suitable under the present scenario, with this suitable area projected to increase by 145.16% in future climatic conditions. States such as Madhya Pradesh, Uttar Pradesh, and Assam are projected to experience an increase in habitat suitability, whereas Odisha and Rajasthan are anticipated to face declines. The study recommends conducting ground-truthing ecological assessments using advanced technologies and genetic analyses to validate the viability of newly identified habitats in the Lower Ganges, Mahanadi, and Brahmaputra River systems. These areas should be prioritized within the Protected Area network for potential translocation sites allocation. Collaborative efforts between the IUCN-SSC Crocodile Specialist Group and stakeholders are vital for prioritizing conservation and implementing site-specific interventions to protect the highly threatened gharial population in the wild. Full article

The American crocodile (Crocodylus acutus, Cuvier, 1807) (Class Reptilia, Family Crocodylidae) is a crocodile species inhabiting the Neotropics. Congenital defects have been described in almost every vertebrate group. In crocodiles, teratology alterations have been described in captive animals (pets, zoos, farms) such as Crocodylus niloticus or Gavialis gangeticus. The present study aimed to characterize congenital malformations of C. acutus from a farm in Lomas de Matunilla, Ballestas, Bolívar, Colombia. A total of 550 unhatched eggs were examined after embryo death. A total of 61 embryos presented malformations, with 42 different types of anomalies observed. Limb and tail malformations (29%) were the most common malformations observed. Several malformations, such as cephalothoracopagus, thoracopagus, sternopagus, xiphopagus twins, campylorrachis scoliosa, and acrania, were documented in crocodiles for the first time. Research in teratology enhances our understanding of crocodile biology. It plays a role in their conservation and management, thus helping to ensure the long-term viability of these species in their natural habitats. Full article

Diabetes mellitus, a metabolic disorder, elevates blood sugar levels, resulting in complications affecting various bodily systems such as the nerves, eyes, kidneys, and other organs. The administration of oral antihyperglycemic medications can potentially induce adverse effects, especially when combined, leading to drug–drug interactions. To address these challenges, there is potential in introducing innovative antioxidant compounds targeted at specific organs for therapeutic action. Currently, metal nanoparticles have gained prominence in healthcare due to their exceptional biocompatibility, stability, cost-effectiveness, and environmentally friendly attributes. In this study, green synthesis methods were employed to produce gold nanoparticles (AuNPs) using the Amaranthus gangeticus plant, known for its antidiabetic properties. The leaf extract of A. gangeticus was utilized to synthesize these green AuNPs using a 1 mM gold chloride solution. Various characterization techniques, including UV spectroscopy, FTIR analysis, X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), and scanning electron microscopy (SEM), were employed to analyse the synthesized herbal-mediated AuNPs. Additionally, the in vivo antidiabetic efficacy of the produced AuNPs was evaluated. The induction of diabetes using STZ resulted in elevated levels of blood glucose, cholesterol, triglycerides, LDL, and VLDL and a significant loss in body weight. However, these detrimental effects were mitigated after treating diabetic rats for 28 days, with significant improvements observed at a dosage range of 1 mg/kg of AuNPs compared to the group treated solely with the plant extract. The findings suggest that the plant-mediated AuNPs demonstrate significant potential as antidiabetic agents compared to the crude extract alone. Full article

Previous studies have shown that salinity eustress enhances the nutritional and bioactive compounds and antiradical capacity (ARC) of vegetables and increases the food values for nourishing human diets. Amaranth is a salinity-resistant, rapidly grown C₄ leafy vegetable with diverse variability and usage. It has a high possibility to enhance nutritional and bioactive compounds and ARC by the application of salinity eustress. Hence, the present study aimed to evaluate the effects of sodium chloride stress response in a selected Lalshak (A. gangeticus) genotype on minerals, ascorbic acid (AsA), Folin–Ciocalteu reducing capacity, beta-carotene (BC), total flavonoids (TF), pigments, polyphenolic profiles, and ARC. A high-yield, high-ARC genotype (LS6) was grown under conditions of 0, 25, 50, and 100 mM sodium chloride in four replicates following a block design with complete randomization. We recognized nine copious polyphenolic compounds in this accession for the first time. Minerals, Folin–Ciocalteu reducing capacity, AsA, BC, pigments, polyphenolic profiles, and ARC of Lalshak were augmented progressively in the order: 0 < 25 < 50 < 100 mM sodium chloride. At 50 mM and 100 mM salt concentrations, minerals, AsA, Folin–Ciocalteu reducing capacity, BC, TF, pigments, polyphenolic profiles, and ARC of Lalshak were much greater than those of the control. Lalshak could be used as valuable food for human diets as a potent antioxidant. Sodium chloride-enriched Lalshak provided outstanding quality to the final product in terms of minerals, AsA, Folin–Ciocalteu reducing capacity, BC, TF, pigments, polyphenolic profiles, and ARC. We can cultivate it as a promising alternative crop in salinity-prone areas of the world. Full article

Earlier researchers have highlighted the utilization of salt eustress for boosting the nutritional and phenolic acid (PA) profiles and antiradical potential (ARP) of vegetables, which eventually boost food values for nourishing human diets. Amaranth is a rapidly grown, diversely acclimated C₄ leafy vegetable with climate resilience and salinity resistance. The application of salinity eustress in amaranth has a great scope to augment the nutritional and PA profiles and ARP. Therefore, the A. gangeticus genotype was evaluated in response to salt eustress for nutrients, PA profile, and ARP. Antioxidant potential and high-yielding genotype (LS1) were grown under four salt eustresses (control, 25 mM, 50 mM, 100 mM NaCl) in a randomized completely block design (RCBD) in four replicates. Salt stress remarkably augmented microelements, proximate, macro-elements, phytochemicals, PA profiles, and ARP of A. gangeticus leaves in this order: control < low sodium chloride stress (LSCS) < moderate sodium chloride stress (MSCS) < severe sodium chloride stress (SSCS). A large quantity of 16 PAs, including seven cinnamic acids (CAs) and nine benzoic acids (BAs) were detected in A. gangeticus genotypes. All the microelements, proximate, macro-elements, phytochemicals, PA profiles, and ARP of A. gangeticus under MSCS, and SSCS levels were much higher in comparison with the control. It can be utilized as preferential food for our daily diets as these antiradical compounds have strong antioxidants. Salt-treated A. gangeticus contributed to excellent quality in the end product in terms of microelements, proximate, macro-elements, phytochemicals, PA profiles, and ARP. A. gangeticus can be cultivated as an encouraging substitute crop in salt-affected areas of the world. Full article

Amphibians and reptiles have interacted with humans for millennia. However, humans interact with amphibian and reptile species in different manners, which depend on their culture and traditions. This study was designed to better understand the interactions between amphibian and reptile species and their usage among the native peoples in the vicinity of the Jhelum and Chenab rivers, Pakistan. Information was collected through semi-structured interviews and questionnaires, and was analyzed by using different indices, including the frequency of citation, corrected fidelity level, fidelity level, relative importance level, and informant major ailment. Two amphibians and twenty-six reptile species were used in therapeutic medicine in the study area. Based on the cultural analysis, we found that Naja naja (black cobra) was highly cited across all cultural groups. A 100% Fidelity Level was calculated for the following species: Naja naja (eye infection), Varanus bengalensis (joint pain), Eurylepis taeniolatus (cataract), and Acanthodactylus cantoris (cancer). We found five endangered species in the study area, i.e., Aspideretes gangeticus, A. hurum, Chitra indica, Varanus flavescens, and Geoclemys hamiltonii, that were used to cure joint pain, muscle stretching and pain, backbone pain, paralysis, and psoriasis, respectively. Likewise, Lissemys punctata andersoni, a vulnerable species as labelled by the International Union for Conservation of Nature, was extensively used for the treatment of joint pain, body pain, paralysis, and arthritis in the study area. In terms of conservation, it is critical to protect the highly vulnerable and endangered species that are being used in therapeutic medicines. Our findings may be helpful for the conservation of amphibian and reptile species by helping to make an effective plan to prevent their extinction. The main threats to the diversity of amphibian and reptile species in the area are hunting, trading, and cultural use. These threats could potentially lead to the extinction of these species. Therefore, with the involvement of concerned authorities, e.g., local stakeholders, the Ministry of Climate Change, provincial wildlife departments, academia, and conservation managers, immediate conservation measures should be taken for the protection and sustainable utilization of medicinal species. Full article

Natural product-based herbicides could be the effective alternatives to synthetic chemical herbicides for eco-friendly weed management. This research, therefore, was conducted to identify the phytotoxic properties of Parthenium hysterophorus L., Cleome rutidosperma DC. and Borreria alata (Aubl.) DC. with a view to introducing them as a tool for natural herbicide development. The methanol extracts of these plants were examined on the germination and growth of Zea mays L., Oryza sativa L., Abelmoschus esculentus (L.) Moench and Amaranthus gangeticus L., Oryza sativa f. Spontanea Roshev. (Weedy rice), Echinochloa colona (L.) Link., Euphorbia hirta L., and Ageratum conyzoides L. under laboratory and glasshouse conditions. A complete randomized design (CRD) with five replications and randomized complete block design (RCBD) with four replications were laid out for laboratory and glasshouse experiments, respectively. In the laboratory experiment, three plant extracts of 0, 6.25, 12.5, 50, and 100 g L⁻¹ were tested on survival rate, hypocotyl, and radicle length of eight test plant species. No seed germination of A. conzyoides, E. hirta, and A. gangeticus were recorded when P. hysterophorus extract was applied at 50 g L⁻¹. C. rutidosperma had the same effect on those plants at 100 g L⁻¹. In the glasshouse, similar extracts and concentrations used in the laboratory experiments were sprayed on at the 2–3 leaf stage for grasses and 4–6 for the broadleaf species. Tested plants were less sensitive to C. rutidosperma and B. alata compared to P. hysterophorus extract. Among the weeds and crops, A. conyzoides, E. hirta, A. esculentus and A. gangeticus were mostly inhibited by P. hysterophorus extract at 100 g L⁻¹. Based on these results, P. hysterophorus was the most phytotoxic among the tested plant extracts and could be used for developing a new natural herbicide for green agriculture. Full article