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Authors = Zhenshan Lin

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19 pages, 6978 KiB  
Article
Phenotypic Characters and Inheritance Tendency of Agronomic Traits in F1 Progeny of Chinese Cherry
by Zhenshan Liu, Shuaiwei Yang, Lisu Hao, Hao Wang, Jing Zhang, Wen He, Mengyao Li, Yuanxiu Lin, Yunting Zhang, Qing Chen, Yong Zhang, Ya Luo, Haoru Tang, Yan Wang and Xiaorong Wang
Agronomy 2024, 14(12), 2862; https://doi.org/10.3390/agronomy14122862 - 30 Nov 2024
Cited by 2 | Viewed by 852
Abstract
Chinese cherry [Prunus. pseudocerasus Lindl., syn. Cerasus. pseudocerasus (Lindl.) G.Don], an economically important tetraploid fruit crop native to southwestern China, is celebrated as “the earliest fruit of spring”. Understanding the inheritance and heterosis of major agronomical traits is essential for advancing its [...] Read more.
Chinese cherry [Prunus. pseudocerasus Lindl., syn. Cerasus. pseudocerasus (Lindl.) G.Don], an economically important tetraploid fruit crop native to southwestern China, is celebrated as “the earliest fruit of spring”. Understanding the inheritance and heterosis of major agronomical traits is essential for advancing its breeding. In this study, we conducted a three-year observation and inheritance analysis of 32 economic traits in the reciprocal F1 populations (NH, n = 114; HN, n = 87) derived from Chinese cherry landraces “Nanzaohong” and “Hongfei”. The results revealed a broad segregation for all traits in F1 offspring. Fruit size exhibited an inheritance tendency toward smaller dimensions, with some individuals displaying extreme values (Fruit weight, HH = 3.90~12.15%) that highlighted the potential for selecting larger fruits. The hybrids showed a tendency for sweeter fruit flavor, with total soluble solids (RHm = 7.00~19.35%) and soluble sugar (RHm = 11.09% and 17.47%) exhibiting hybrid vigor, along with a decreasing tendency in titratable acid (RHm = −16.08~−1.05%). The flowering and fruiting phenology tended to occur earlier, with extremely early and late flowering lines offering the potential to extend the ornamental and harvesting periods. Fruit bitterness (H2 = 0.98 and 0.95) and fruit skin color (H2 = 0.93 and 0.89) displayed the highest heritability. Correlation analysis revealed strong internal correlations among trait categories, confirming the reliability of the data collection and analysis. Moreover, no significant differences were observed between the maternal and the paternal effect on the inheritance for agronomic traits attributes. This study systematically clarifies the inheritance trends of agronomic traits in Chinese cherry, providing a foundation for the rational selection of parental lines in breeding strategies and laying the groundwork for future molecular genetic research. Full article
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13 pages, 10386 KiB  
Article
Autotetraploid Origin of Chinese Cherry Revealed by Chromosomal Karyotype and In Situ Hybridization of Seedling Progenies
by Yan Wang, Xueou Li, Yan Feng, Juan Wang, Jing Zhang, Zhenshan Liu, Hao Wang, Tao Chen, Wen He, Zhiwei Wu, Yuanxiu Lin, Yunting Zhang, Mengyao Li, Qing Chen, Yong Zhang, Ya Luo, Haoru Tang and Xiaorong Wang
Plants 2023, 12(17), 3116; https://doi.org/10.3390/plants12173116 - 30 Aug 2023
Cited by 9 | Viewed by 2062
Abstract
Polyploidy is considered a driving force in plant evolution and diversification. Chinese cherry [Cerasus pseudocerasus (Lindl.) G.Don], an economically important fruit crop native to China, has evolved at the tetraploid level, with a few pentaploid and hexaploid populations. However, its auto- or [...] Read more.
Polyploidy is considered a driving force in plant evolution and diversification. Chinese cherry [Cerasus pseudocerasus (Lindl.) G.Don], an economically important fruit crop native to China, has evolved at the tetraploid level, with a few pentaploid and hexaploid populations. However, its auto- or allo-polyploid origin remains unclear. To address this issue, we analyzed the ploidy levels and rDNA chromosomal distribution in self- and open-pollinated seedling progenies of tetraploid and hexaploid Chinese cherry. Genomic in situ hybridization (GISH) analysis was conducted to reveal the genomic relationships between Chinese cherry and diploid relatives from the genus Cerasus. Both self- and open-pollinated progenies of tetraploid Chinese cherry exhibited tetraploids, pentaploids, and hexaploids, with tetraploids being the most predominant. In the seedling progenies of hexaploid Chinese cherry, the majority of hexaploids and a few pentaploids were observed. A small number of aneuploids were also observed in the seedling progenies. Chromosome 1, characterized by distinct length characteristics, could be considered the representative chromosome of Chinese cherry. The basic Chinese cherry genome carried two 5S rDNA signals with similar intensity, and polyploids had the expected multiples of this copy number. The 5S rDNA sites were located at the per-centromeric regions of the short arm on chromosomes 4 and 5. Three 45S rDNA sites were detected on chr. 3, 4 and 7 in the haploid complement of Chinese cherry. Tetraploids exhibited 12 signals, while pentaploids and hexaploids showed fewer numbers than expected multiples. Based on the GISH signals, Chinese cherry demonstrated relatively close relationships with C. campanulata and C. conradinae, while being distantly related to another fruiting cherry, C. avium. In combination with the above results, our findings suggested that Chinese cherry likely originated from autotetraploidy. Full article
(This article belongs to the Special Issue Plant Taxonomy, Systematics, and Phylogeography)
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18 pages, 4167 KiB  
Article
Optimal Scheduling of Power Systems with High Proportions of Renewable Energy Accounting for Operational Flexibility
by Yi Lin, Wei Lin, Wei Wu and Zhenshan Zhu
Energies 2023, 16(14), 5537; https://doi.org/10.3390/en16145537 - 21 Jul 2023
Cited by 6 | Viewed by 2006
Abstract
The volatility and uncertainty of high-penetration renewable energy pose significant challenges to the stability of the power system. Current research often fails to consider the insufficient system flexibility during real-time scheduling. To address this issue, this paper proposes a flexibility scheduling method for [...] Read more.
The volatility and uncertainty of high-penetration renewable energy pose significant challenges to the stability of the power system. Current research often fails to consider the insufficient system flexibility during real-time scheduling. To address this issue, this paper proposes a flexibility scheduling method for high-penetration renewable energy power systems that considers flexibility index constraints. Firstly, a quantification method for flexibility resources and demands is introduced. Then, considering the constraint of the flexibility margin index, optimization scheduling strategies for different time scales, including day-ahead scheduling and intra-day scheduling, are developed with the objective of minimizing total operational costs. The intra-day optimization is divided into 15 min and 1 min time scales, to meet the flexibility requirements of different time scales in the power system. Finally, through simulation studies, the proposed strategy is validated to enhance the system’s flexibility and economic performance. The daily operating costs are reduced by 3.1%, and the wind curtailment rate is reduced by 4.7%. The proposed strategy not only considers the economic efficiency of day-ahead scheduling but also ensures a sufficient margin to cope with the uncertainty of intra-day renewable energy fluctuations. Full article
(This article belongs to the Special Issue Intelligent Analysis and Control of Modern Power Systems)
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20 pages, 10849 KiB  
Article
Integrated Transcriptome and Metabolome Analyses Provide Insights into the Coloring Mechanism of Dark-red and Yellow Fruits in Chinese Cherry [Cerasus pseudocerasus (Lindl.) G. Don]
by Yan Wang, Zhiyi Wang, Jing Zhang, Zhenshan Liu, Hao Wang, Hongxia Tu, Jingting Zhou, Xirui Luo, Qing Chen, Wen He, Shaofeng Yang, Mengyao Li, Yuanxiu Lin, Yunting Zhang, Yong Zhang, Ya Luo, Haoru Tang and Xiaorong Wang
Int. J. Mol. Sci. 2023, 24(4), 3471; https://doi.org/10.3390/ijms24043471 - 9 Feb 2023
Cited by 13 | Viewed by 2652
Abstract
Chinese cherry [Cerasus pseudocerasus (Lindl.) G. Don] is an important fruit tree from China that has excellent ornamental, economic, and nutritional values with various colors. The dark-red or red coloration of fruit, an attractive trait for consumers, is determined by anthocyanin pigmentation. [...] Read more.
Chinese cherry [Cerasus pseudocerasus (Lindl.) G. Don] is an important fruit tree from China that has excellent ornamental, economic, and nutritional values with various colors. The dark-red or red coloration of fruit, an attractive trait for consumers, is determined by anthocyanin pigmentation. In this study, the coloring patterns during fruit development in dark-red and yellow Chinese cherry fruits were firstly illustrated by integrated transcriptome and widely-targeted metabolome analyses. Anthocyanin accumulation in dark-red fruits was significantly higher compared with yellow fruits from the color conversion period, being positively correlated to the color ratio. Based on transcriptome analysis, eight structural genes (CpCHS, CpCHI, CpF3H, CpF3’H, CpDFR, CpANS, CpUFGT, and CpGST) were significantly upregulated in dark-red fruits from the color conversion period, especially CpANS, CpUFGT, and CpGST. On contrary, the expression level of CpLAR were considerably higher in yellow fruits than in dark-red fruits, especially at the early stage. Eight regulatory genes (CpMYB4, CpMYB10, CpMYB20, CpMYB306, bHLH1, CpNAC10, CpERF106, and CpbZIP4) were also identified as determinants of fruit color in Chinese cherry. Liquid chromatography-tandem mass spectrometry identified 33 and 3 differential expressed metabolites related to anthocyanins and procyanidins between mature dark-red and yellow fruits. Cyanidin-3-O-rutinoside was the predominant anthocyanin compound in both fruits, while it was 6.23-fold higher in dark-red than in yellow fruits. More accumulated flavanol and procyanidin contents resulted in less anthocyanin content in flavonoid pathway in yellow fruits due to the higher expression level of CpLAR. These findings can help understand the coloring mechanism of dark-red and yellow fruits in Chinese cherry, and provide genetic basis for breeding new cultivars. Full article
(This article belongs to the Special Issue Advances in Research for Horticultural Crops Breeding and Genetics)
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16 pages, 7121 KiB  
Article
Expression and Regulation of a Novel Decidual Cells-Derived Estrogen Target during Decidualization
by Lin Lu, Yingni Chen, Zhenshan Yang, Shijin Liang, Songqi Zhu and Xiaohuan Liang
Int. J. Mol. Sci. 2023, 24(1), 302; https://doi.org/10.3390/ijms24010302 - 24 Dec 2022
Cited by 7 | Viewed by 3065
Abstract
During decidualization in rodents, uterine stromal cells undergo extensive reprogramming to differentiate into distinct cell types, forming primary decidual zones (PDZs), secondary decidual zones (SDZs), and layers of undifferentiated stromal cells. The formation of secondary decidual zones is accompanied by extensive angiogenesis. During [...] Read more.
During decidualization in rodents, uterine stromal cells undergo extensive reprogramming to differentiate into distinct cell types, forming primary decidual zones (PDZs), secondary decidual zones (SDZs), and layers of undifferentiated stromal cells. The formation of secondary decidual zones is accompanied by extensive angiogenesis. During early pregnancy, besides ovarian estrogen, de novo synthesis of estrogen in the uterus is essential for the progress of decidualization. However, the molecular mechanisms are not fully understood. Studies have shown that Cystatin B (Cstb) is highly expressed in the decidual tissue of the uterus, but the regulation and mechanism of Cstb in the process of decidualization have not been reported. Our results showed that Cstb was highly expressed in mouse decidua and artificially induced deciduoma via in situ hybridization and immunofluorescence. Estrogen stimulates the expression of Cstb through the Estrogen receptor (ER)α. Moreover, in situ synthesis of estrogen in the uterus during decidualization regulates the expression of Cstb. Silencing the expression of Cstb affects the migration ability of stromal cells. Knockdown Cstb by siRNA significantly inhibits the expression of Dtprp, a marker for mouse decidualization. Our study identifies a novel estrogen target, Cstb, during decidualization and reveals that Cstb may play a pivotal role in angiogenesis during mouse decidualization via the Angptl7. Full article
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15 pages, 8095 KiB  
Article
Adaptive Control Strategy of Energy Storage System Participating in Primary Frequency Regulation
by Chaoxiong Fang, Yuchen Tang, Rong Ye, Zhangsui Lin, Zhenshan Zhu, Buying Wen and Chengtao Ye
Processes 2020, 8(6), 687; https://doi.org/10.3390/pr8060687 - 12 Jun 2020
Cited by 18 | Viewed by 3737
Abstract
In order to solve the capacity shortage problem in power system frequency regulation caused by large-scale integration of renewable energy, the battery energy storage-assisted frequency regulation is introduced. In this paper, an adaptive control strategy for primary frequency regulation of the energy storage [...] Read more.
In order to solve the capacity shortage problem in power system frequency regulation caused by large-scale integration of renewable energy, the battery energy storage-assisted frequency regulation is introduced. In this paper, an adaptive control strategy for primary frequency regulation of the energy storage system (ESS) was proposed. The control strategy combined virtual droop control, virtual inertial control, and virtual negative inertial control. The virtual inertial control was used to reduce the frequency change rate, and the virtual droop control was used to reduce the steady-state frequency deviation. The virtual droop control and the virtual inertia control were adopted in the frequency deterioration stage to slow down the frequency drop. While in the frequency recovery stage, the virtual negative inertia control worked together with the virtual droop control to accelerate the frequency recovery. Besides, the coefficients of the control methods were related to the state of charge (SOC) of ESS to avoid over-charging and over-discharging of the battery. Finally, a simulation model was built in MATLAB/SIMULINK, and case studies were conducted to verify the proposed control strategy. Results showed that the proposed method could meet the demand for frequency regulation and was beneficial to the cycle life of ESS. Full article
(This article belongs to the Special Issue Energy Storage System: Integration, Power Quality, and Operation)
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23 pages, 5390 KiB  
Article
Sulfide Rb-Sr, Re-Os and In Situ S Isotopic Constraints on Two Mineralization Events at the Large Hongnipo Cu Deposit, SW China
by Lujun Lin, Renyi Chen, Zhenshan Pang, Hui Chen, Jianling Xue and Hongxiang Jia
Minerals 2020, 10(5), 414; https://doi.org/10.3390/min10050414 - 7 May 2020
Cited by 11 | Viewed by 3691
Abstract
The Hongnipo deposit, a newly discovered large copper deposit in the Kangdian copper belt, SW China, is hosted in the Paleoproterozoic Hekou Group. This deposit contains ~4200 Mt of Cu ores, with an average grade of 1.42 wt.% Cu. Orebodies occur mainly as [...] Read more.
The Hongnipo deposit, a newly discovered large copper deposit in the Kangdian copper belt, SW China, is hosted in the Paleoproterozoic Hekou Group. This deposit contains ~4200 Mt of Cu ores, with an average grade of 1.42 wt.% Cu. Orebodies occur mainly as stratiform, stratoid and lenticular forms. Mineralization consists predominantly of high grade vein-type ores and low grade laminated ores. Field relationships indicate vein-type mineralization crosscuts laminated mineralization and host rocks, indicating that there were at least two mineralization events during the formation of the deposit. Pyrite separates from the laminated ores yield a Rb-Sr isochron age of 1552 ± 80 Ma, with a highly radiogenic initial 87Sr/86Sr ratio of 0.71214 ± 0.00081, indicating a major contribution from the ore-hosting rocks. Sulfides from the laminated ores have δ34S values ranging from −1.8‰ to 11.4‰, with the vast majority in the range of 5.3‰ to 11.4‰, suggesting the mixed derivation of sulfur from seawater sulfates and magmatic fluids. Chalcopyrite separates from the vein-type ores have a Re-Os isochron age of 794.8 ± 7.9 Ma. The initial 187Os/188Os (2.8 ± 1.2) and γOs (+2202) values are slightly lower than the average values of continental crust, indicating a major metal source of the Hekou Group with minor mantle input. Sulfides from the vein-type ores have δ34S values that range from −10.3‰ to 4.0‰ and cluster between 0‰ to 2.2‰, which implies a significant contribution of magmatic-sourced sulfur with minor biogenic sulfur. Two major mineralization events have been identified. The Rb-Sr age of the laminated ores likely records a VMS mineralization event at ~1.6 Ga. The much younger Re-Os age is considered to represent the timing of an important mineralization event, which is likely related to the Neoproterozoic magmatism and/or metamorphism and represents a newly documented mineralization event to be targeted by exploration. Full article
(This article belongs to the Special Issue Integrated Chronology Studies of Ore Deposits)
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19 pages, 3184 KiB  
Article
Trend Evolution of Vegetation Phenology in China during the Period of 1981–2016
by Fusheng Jiao, Huiyu Liu, Xiaojuan Xu, Haibo Gong and Zhenshan Lin
Remote Sens. 2020, 12(3), 572; https://doi.org/10.3390/rs12030572 - 8 Feb 2020
Cited by 38 | Viewed by 4498
Abstract
The trend of vegetation phenology dynamics is of crucial importance for understanding vegetation growth and its responses to climate change. However, it remains unclear how the trends of vegetation phenology changed over the past decades. By analyzing phenology data including start (SOS), end [...] Read more.
The trend of vegetation phenology dynamics is of crucial importance for understanding vegetation growth and its responses to climate change. However, it remains unclear how the trends of vegetation phenology changed over the past decades. By analyzing phenology data including start (SOS), end (EOS) and length (LOS) of growth season with the Ensemble empirical mode decomposition (EEMD), we revealed the trend evolution of vegetation phenology in China during 1981-2016. Our study suggests that: (1) On the national scale, with EEMD method, the change rates of SOS and LOS were increasing with time, while that of EOS was decreasing. Moreover, the EEMD rates of SOS and LOS exceeded the linear rates in the early-2000s, while that of EOS dropped below the linear rate in the mid-1980s. (2) For each phenological event, the shifted trends took up a large area (~30%), which was close to the sum of all monotonic trends, but more than any monotonic trend type. The shifted trends mainly occurred in the north-eastern China, eastern Qinghai-Tibetan Plateau, eastern Sichuan Basin, North China Plain and Loess Plateau. (3) For each phenological event, the areas in the high-latitude experienced the contrary trends to the other. The amplitude and frequencies of phenology variation in the mid-latitude were stronger than those in the high-latitude and low-latitude. Meanwhile, LOS in the high-latitude was induced by SOS. (4) For each phenological event, the trend evolution varying with longitudes can be divided into eastern region (east of 121°E), central region (92°E–121°E) and western region (west of 92°E) based on the evolution of trends varying with longitudes. The east experienced a delayed SOS and a shorten LOS, which was different from the other areas. The magnitude of delayed trends in EOS and the prolonged trends in LOS were stronger from east to west as longitudes changes. The variation characteristics of LOS with longitude were mainly caused by SOS in the eastern region and by SOS and EOS together in the western and central region. (5) Each land cover types, except Needleleaf Forests, experienced the same trends. For most land cover types, the advance of SOS, delay of EOS and extension of LOS began in the 1980s, the 1990s, and the 1990s, respectively and enhanced several times. Moreover, the magnitudes of Grasslands in SOS and Evergreen Broadleaf Forest in EOS were much greater than the others, while that of croplands was the smallest in each phenological event. Our results showed that the analysis of trend evolution with nonlinear method is very important to accurately reveal the variation characteristics of phenology trends and to extract the inherent trend shifts. Full article
(This article belongs to the Special Issue Monitoring Vegetation Phenology: Trends and Anomalies)
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22 pages, 2736 KiB  
Article
Relationship of Abrupt Vegetation Change to Climate Change and Ecological Engineering with Multi-Timescale Analysis in the Karst Region, Southwest China
by Xiaojuan Xu, Huiyu Liu, Zhenshan Lin, Fusheng Jiao and Haibo Gong
Remote Sens. 2019, 11(13), 1564; https://doi.org/10.3390/rs11131564 - 2 Jul 2019
Cited by 46 | Viewed by 4223
Abstract
Vegetation is known to be sensitive to both climate change and anthropogenic disturbance in the karst region. However, the relationship between an abrupt change in vegetation and its driving factors is unclear at multiple timescales. Based on the non-parametric Mann-Kendall test and the [...] Read more.
Vegetation is known to be sensitive to both climate change and anthropogenic disturbance in the karst region. However, the relationship between an abrupt change in vegetation and its driving factors is unclear at multiple timescales. Based on the non-parametric Mann-Kendall test and the ensemble empirical mode decomposition (EEMD) method, the abrupt changes in vegetation and its possible relationships with the driving factors in the karst region of southwest China during 1982–2015 are revealed at multiple timescales. The results showed that: (1) the Normalized Difference Vegetation Index (NDVI) showed an overall increasing trend and had an abrupt change in 2001. After the abrupt change, the greening trend of the NDVI in the east and the browning trend in the west, both changed from insignificant to significant. (2) After the abrupt change, at the 2.5-year time scale, the correlation between the NDVI and temperature changed from insignificantly negative to significantly negative in the west. Over the long-term trend, it changed from significantly negative to significantly positive in the east, but changed from significantly positive to significantly negative in the west. The abrupt change primarily occurred on the long-term trend. (3) After the abrupt change, 1143.32 km2 farmland was converted to forests in the east, and the forest area had significantly increased. (4) At the 2.5-year time scale, the abrupt change in the relationships between the NDVI and climate factors was primarily driven by climate change in the west, especially rising temperatures. Over the long-term trend, it was caused by ecological protection projects in the east, but by rising temperatures in the west. The integration of the abrupt change analysis and multiple timescale analysis help assess the relationship of vegetation changes with climate changes and human activities accurately and comprehensively, and deepen our understanding of the driving mechanism of vegetation changes, which will further provide scientific references for the protection of fragile ecosystems in the karst region. Full article
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30 pages, 36583 KiB  
Article
Appling the One-Class Classification Method of Maxent to Detect an Invasive Plant Spartina alterniflora with Time-Series Analysis
by Xiang Liu, Huiyu Liu, Haibo Gong, Zhenshan Lin and Shicheng Lv
Remote Sens. 2017, 9(11), 1120; https://doi.org/10.3390/rs9111120 - 4 Nov 2017
Cited by 39 | Viewed by 7845
Abstract
Spartina alterniflora has become the main invasive plant along the Chinese coast and now threatens the local ecological environment. Accurately monitoring the distribution of S. alterniflora is urgent and essential for developing cost-effective control strategies. In this study, we applied the One-Class Classification [...] Read more.
Spartina alterniflora has become the main invasive plant along the Chinese coast and now threatens the local ecological environment. Accurately monitoring the distribution of S. alterniflora is urgent and essential for developing cost-effective control strategies. In this study, we applied the One-Class Classification (OCC) methods of Maximum entropy (Maxent) and Biased Support Vector Machine (BSVM) based on Landsat time-series imagery to detect the species on the middle coast of Jiangsu in east China. We conducted four experimental setups (i.e., single-scene analysis, time-series analysis, Normalized Difference Vegetation Index (NDVI) time-series analysis and a compressed time-series analysis), using OCC methods to recognize the species. Then, we tested the performance of a compressed time-series model for S. alterniflora detection and evaluated the expansibility of this approach when it was applied to a larger region. Our principal findings are as follows: (1) Maxent and BSVM performed equally well, and Maxent appeared to have a more balanced performance over the summer months; (2) the Maxent model with the Default Parameter Set (Maxent-DPS) showed a slightly higher accuracy and more overfitting than Maxent with the Akaike Information Criterion corrected for small samples sizes (AICc)-selected parameter set model, but a t-test found no significant difference between these two settings; (3) April and December were deemed to be important periods for the detection of S. alterniflora; (4) a compressed time-series analysis model—including only three variables (December NDVI, March green and the third Principal Component in January, PC3)—yielded higher accuracy than single-scene analyses, which indicated that time-series analysis can better detect S. alterniflora than single-scene analyses; and (5) the Maxent model using the reconstructed optimal variables and 70 training samples over a larger region produced encouraging results with an overall accuracy of 90.88% and a Kappa of 0.78. The one-class classification method combined with a phenology-based detection strategy is therefore promising for the application of the long-term detection of S. alterniflora over extended areas. Full article
(This article belongs to the Section Remote Sensing in Agriculture and Vegetation)
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16 pages, 1743 KiB  
Article
Using a Coupled Human-Natural System to Assess the Vulnerability of the Karst Landform Region in China
by Xiang He, Zhenshan Lin and Kangning Xiong
Sustainability 2015, 7(9), 12910-12925; https://doi.org/10.3390/su70912910 - 18 Sep 2015
Cited by 4 | Viewed by 5261
Abstract
Guizhou Plateau is a region in China that typically shows the contradictory human-earth system. A vulnerability assessment indicator system was constructed to explore the coupled human-natural system characteristic of the karst landform based on the grey correlation analysis mathematic model. The quantitative assessment [...] Read more.
Guizhou Plateau is a region in China that typically shows the contradictory human-earth system. A vulnerability assessment indicator system was constructed to explore the coupled human-natural system characteristic of the karst landform based on the grey correlation analysis mathematic model. The quantitative assessment results show that Qiandongnan and Tongren Districts belong to the slight degree of the sensitivity evaluation index. Bijie district belongs to the middle degree and the other districts of Guizhou Plateau belong to the light degree. In terms of the exposure and resilience evaluation index, only Guiyang City belongs to the slight degree and other districts are in the middle degree. Thus, Guizhou Plateau could be divided into three level zones based on the comprehensive vulnerability degree of the coupled human-natural system. The strong degree vulnerability zone includes Liupanshui City, Bijie City, Anshun City, and Qiannan District. The middle degree vulnerability zone includes the districts of Qiandongnan, Qianxinan, and Tongren and the city of Zunyi. The slight degree vulnerability zone only includes Guiyang City. The research results suggest that the coupled human-natural system in Guizhou Plateau has a high vulnerability. Full article
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