Search Results (36)

Opioids are among the most effective drugs for treating moderate to severe pain. Unfortunately, opioid use, even short-term, can lead to addiction, tolerance, overdose, and respiratory depression. Therefore, efforts to design and develop novel compounds that would retain analgesic activity while reducing side effects continue unabated. The present study was designed to investigate the respiratory and cardiovascular effects of the hybrid peptide LENART01, which has evidenced potent antinociceptive and antimicrobial activity. This hybrid peptide, composed of N-terminally located dermorphin and C-terminal modified ranatensin pharmacophore, was tested in vivo in anesthetized rats. The main effect of LENART01 was apnea in 70% of examined animals, sighing, and a significant increase in blood pressure. Interestingly, the hybrid induced sighs less frequently than ranatensin, and apnea dependent on vagus nerve mu opioid receptor activation much less frequently and less intensely than dermorphin itself. This shows that LENART01 is a safer opioid system-related agent as compared to dermorphin for its prospective use in the treatment of pain. Full article

The aging process significantly impacts lung physiology and is a major risk factor for chronic respiratory diseases, including chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), asthma, and non-IPF interstitial lung fibrosis. This narrative clinical review explores the molecular and biochemical hallmarks of aging, such as oxidative stress, telomere attrition, genomic instability, epigenetic modifications, proteostasis loss, and impaired macroautophagy, and their roles in lung senescence. Central to this process are senescent cells, which, through the senescence-associated secretory phenotype (SASP), contribute to chronic inflammation and tissue dysfunction. The review highlights parallels between lung aging and pathophysiological changes in respiratory diseases, emphasizing the role of cellular senescence in disease onset and progression. Despite promising research into modulating aging pathways with interventions like caloric restriction, mTOR inhibitors, and SIRT1 activators, clinical evidence for efficacy in reversing or preventing age-related lung diseases remains limited. Understanding the interplay between aging-related mechanisms and environmental factors, such as smoking and pollution, is critical for developing targeted therapies. This review underscores the need for future studies focusing on therapeutic strategies to mitigate aging’s detrimental effects on lung health and improve outcomes for patients with chronic respiratory conditions. Full article

Background and Objectives: In recent years, there has been a significant increase in interest in the health benefits of contact with nature. The theoretical and practical aspects of this issue are constantly being investigated. It is worth noting, however, that there is a large group of people for whom the only available form of contact with nature can only be through images (the view outside the window, photos and films about nature), recorded sounds of nature or descriptions of nature. Research indicating how much exposure to nature in the dimension of virtual reality achieves improvements in well-being, mood and vitality is therefore urgently needed. Material and Methods: The main goal of the experiment was to examine whether watching a 20-min 2D video of forest environment or reading a textbook on forest knowledge for 20 min would bring any psychological benefits. The order of both exposures was intentionally changed (reversed) for every second participant in this study to reduce the order effect. A group of 16 volunteers, students of Warsaw University of Life Sciences, took part in the experiment. The psychological tests before and after each of the two parts of the experiment (viewing a picture with sound and reading a book) were established using five questionnaires: Profile of Mood State (POMS); Positive and Negative Affect Scale (PANAS); Restorative Outcome Scale (ROS); Subjective Vitality Scale (SVS) and State-Trait Anxiety Inventory (STAI). Results: As a result of the analyses, a 20-min viewing of a 2D video of a forest with natural sounds of nature was shown to have a positive effect on the psychological relaxation of the subjects in the form of an increase in restorativeness and vitality in the participants of the experiment, as well as a greater decrease in negative feelings, tension and anger than when reading a forest-themed book. Watching a video of a landscape can reduce tension and anger levels significantly, which can be an important guideline in relation to the interior design of public spaces and the protection of employees from the adverse effects of psychosocial working conditions, as well as the desire to stabilize anger and maintain emotional stability in everyday life. Full article

Biometric authentication methods offer high-quality mechanisms to confirm the identity of individuals in security systems commonly used in the modern world, such as physical access control, online banking, or mobile device unlocking. They also find their application in cryptographic solutions, which allow the biometrically authenticated exchange of cryptographic keys between users and services on the internet, despite the fuzziness of biometric data. Such solutions are BAKE (biometrics-authenticated key exchange) and BRAKE (biometric-resilient authenticated key exchange) protocols, upon which our work is based. However, the direct application of fuzzy biometrics in cryptography, which relies heavily on the accuracy of single-bit secret values, is not trivial. Therefore, this paper is devoted to analyzing the security of this idea and the feasibility of implementing biometric AKE (authenticated key exchange) protocols, with an emphasis on the BRAKE protocol. As the results of our analysis, we discuss BRAKE’s limitations and vulnerabilities, which need to be appropriately addressed to implement the protocol in modern systems. Full article

One of the main aspects of the control system development process for direct drives is the selection of the required computational accuracy while establishing its impact on the quality of the control. Understanding this relationship allows designers to consciously determine the system structure at the early stages of controller development, which enables the optimal usage of hardware resources. This paper analyzes the results of experimental research on the influence of computational accuracy on the quality of control of direct drives. During the carried-out research, several vector controllers with different computational precision were implemented using Field-Programmable Gate Arrays (FPGAs). The experiments were carried out on a dedicated research stand for testing direct drives. Test scenarios included position and trajectory monitoring under various torque loads. To assess the control quality, the measures based on the deviations from the value set by the controller were proposed. In this paper, the results of experiments have been presented in the form of values of the measures in relation to the computational accuracy. The obtained results proved that satisfactory drive operation parameters can be obtained despite the relatively low accuracy of calculations in the control algorithm. Full article

Unmanned platforms are becoming more and more widely used in both civilian and military applications. The flight safety of such aircraft is crucial. Therefore, it becomes necessary to formally model and rigorously test their software. Commencement of an unmanned air vehicle’s (UAV) operation requires it undergoing a certification process, which further heightens the quality requirements. The validation process of the designed system is presented on the example of verifying the correctness of the operational scenario involving the transfer of control over a UAV between two ground control stations (GCSs). The paper introduces the method of designing unmanned aerial vehicles and testing their functionality based on finite-state machines. The architecture description of the system encompasses the use cases view and logical view of the 1+5 architectural views model. The paper concentrates on system requirements and their transformation into unified modeling language (UML) state machine diagrams. The authors present a method of mapping requirements to functions related to flight safety and system dynamics models expressed as communicating extended finite-state machines (CEFSMs). Moreover, the paper shows the rules for testing the correctness of state machine models using methods based on determining flows in directed graphs. It should be emphasized that the paper considers the certification of UAV software components from the system safety viewpoint, which directly affects the airworthiness of the air platform. Full article

Recently, many studies have been conducted on the impact of various elements of the natural environment, including forests, on human physical and mental health. However, little is known about the level of health benefits resulting from contact with forests depending on the type of physical activity undertaken. Therefore, in order to measure the impact of physical activity on the level of mental relaxation, a randomized experiment was conducted, which took into account three types of human physical activity: walking, cycling, and passive (without movement) observation of the forest. The study was carried out in the same forest and at the same time. Forty young people studying in Warsaw took part in the study. Four psychological questionnaires were used in the project before and after the experiment (Profile of Mood States, Schedule of Positive and Negative Affects, Recovery Scale, Subjective Vitality Scale). A pre-test was also performed in a university classroom. Research has shown that staying in the forest, regardless of the type of physical activity, brings positive health benefits in the form of an increase in positive feelings while reducing negative feelings. The results indicate that people who walk have the broadest range of benefits (cumulative benefits), in the form of less tension, reduced anger, fatigue, depression, increased concentration and greater vigor. Cyclists experienced significant benefits only in the form of reduced depression and greater vigor. The group passively observing the forest achieved statistically significant benefits only in terms of reducing fatigue and improving concentration. However, overall, the between-group results showed no statistically significant differences between the restorative effects of walking, cycling, and viewing the forest landscape. Each analyzed form of contact with the forest has a regenerating/regenerating effect (ROS scale) and contributes to the increase in vitality (SVS scale). Full article

Canine babesiosis is a disease caused by protozoan pathogens belonging to the genus Babesia. Four species of large Babesia cause canine babesiosis (B. canis, B. rossi, B. vogeli, and the informally named B. coco). Although canine babesiosis has a worldwide distribution, different species occur in specific regions: B. rossi in sub-Saharan Africa, B. canis in Europe and Asia, and B. coco in the Eastern Atlantic United States, while B. vogeli occurs in Africa, southern parts of Europe and Asia, northern Australia, southern regions of North America, and in South America. B. vogeli is the most prevalent large Babesia species globally. This results from its wide range of monotropic vector species, the mild or subclinical nature of infections, and likely the longest evolutionary association with dogs. The most important risk factors for infection by large Babesia spp. include living in rural areas, kennels or animal shelters, or regions endemic for the infection, the season of the year (which is associated with increased tick activity), infestation with ticks, and lack of treatment with acaricides. Full article

The central nervous system manages all of our activities (e.g., direct thinking and decision-making processes). It receives information from the environment and responds to environmental stimuli. Bacterial viruses (bacteriophages, phages) are the most numerous structures occurring in the biosphere and are also found in the human organism. Therefore, understanding how phages may influence this system is of great importance and is the purpose of this review. We have focused on the effect of natural bacteriophages in the central nervous system, linking them to those present in the gut microbiota, creating the gut-brain axis network, as well as their interdependence. Importantly, based on the current knowledge in the field of phage application (e.g., intranasal) in the treatment of bacterial diseases associated with the brain and nervous system, bacteriophages may have significant therapeutic potential. Moreover, it was indicated that bacteriophages may influence cognitive processing. In addition, phages (via phage display technology) appear promising as a targeted therapeutic tool in the treatment of, among other things, brain cancers. The information collected and reviewed in this work indicates that phages and their impact on the nervous system is a fascinating and, so far, underexplored field. Therefore, the aim of this review is not only to summarize currently available information on the association of phages with the nervous system, but also to stimulate future studies that could pave the way for novel therapeutic approaches potentially useful in treating bacterial and non-bacterial neural diseases. Full article

The paper presents a longitudinal cohort survey on the public acceptability of COVID-19 vaccination and real vaccination levels. A survey on a representative sample of adult Polish citizens (n = 1066) was conducted in June 2020 and, one year later, the same group was approached to compare the hypothetical declarations with the real vaccination decisions (n = 438). A significant part of the group that declared reluctance and hesitation toward COVID-19 vaccination before that vaccination was available actually got vaccinated or plans to get vaccinated. Those respondents were asked about the reasons for changing their attitudes. Among the previously vaccine-reluctant individuals, the main reasons included concern about their health and safety (50%) and their desire to travel (26.6%). Vaccine-hesitant individuals also indicated health and safety as their primary concern (69%), as well as the pursuit of herd immunity and a notion of common social safety (12.6%). The main factors helping to increase vaccination acceptance are based on a self-centered pursuit of safety and freedom from restrictions. The survey results may help to prepare a more effective vaccination campaign. Full article

The year 2020 marked 15 years of the Phage Therapy Unit in Poland, the inception of which took place just one year after Poland’s accession to the European Union (2004). At first sight, it is hard to find any connection between these two events, but in fact joining the European Union entailed the need to adapt the regulatory provisions concerning experimental treatment in humans to those that were in force in the European Union. These changes were a solid foundation for the first phage therapy center in the European Union to start its activity. As the number of centers conducting phage therapy in Europe and in the world constantly and rapidly grows, we want to grasp the opportunity to take a closer look at the over 15-year operation of our site by analyzing its origins, legal aspects at the local and international levels and the impressive number and diversity of cases that have been investigated and treated during this time. This article is a continuation of our work published in 2020 summarizing a 100-year history of the development of phage research in Poland. Full article

A two-factor field experiment using sugar beet was conducted in 2016–2018 at Sokołowo in Poland (ϕ 53°5′16.05″ N, λ 19°6′21.07″ E), in a strip-plot arrangement with four replicates. Strip-till (ST) was compared to conventional tillage (CT) using four commercial sugar beet varieties (Alegra, Armesa, Contenta, Julius). In each study year, the experiment was established on lessivé soils developed from heavy loamy sand. The soil was rich in available macronutrients, while its reaction was neutral. The plant density, sugar yield, roots quality, and technological sugar yield were determined. A significant increase was found in root yield (6.6%) and, accordingly, in technological sugar yield (8.2%) in ST treatment relative to CT. Consistently, an increasing trend was observed for the root sugar content (0.21%). For the varieties examined, no preferences were observed in respect of tillage systems applied. The direction of changes in root yield, sugar content, and technological sugar yield remained constant regardless of the variety, tillage system, or the study year. The contents of potassium, sodium, and α-amino-nitrogen responded equally to both the variety and study year; however, the direction of changes in the above parameters was ambiguous and varied among the study years. Full article

The aim of this study was the isolation and characterization, including the phage effect on honeybees in laboratory conditions, of phages active against Paenibacillus larvae, the causative agent of American Foulbrood—a highly infective and easily spreading disease occurring in honeybee larva, and subsequently the development of a preparation to prevent and treat this dangerous disease. From the tested material (over 2500 samples) 35 Paenibacillus spp. strains were obtained and used to search for phages. Five phages specific to Paenibacillus were isolated and characterized (ultrastructure, morphology, biological properties, storage stability, and genome sequence). The characteristics were performed to obtain knowledge of their lytic potential and compose the final phage cocktail with high antibacterial potential and intended use of future field application. Preliminary safety studies have also been carried out on healthy bees, which suggest that the phage preparation administered is harmless. Full article

This study investigated operational and structural health monitoring (SHM) as well as damage evaluations for building structures. The study involved damage detection and the assessment of buildings by placing sensors and by assuming weak areas, and considered situations of assessment and self-monitoring. From this perspective, advanced sensor technology and data acquisition techniques can systematically monitor a building in real time. Furthermore, the structure’s response and behavior were observed and recorded to predict the damage to the building. In this paper, we discuss the real-time monitoring and response of buildings, which includes both static and dynamic analyses along with numerical simulation studies such as finite element analysis (FEA), and recommendations for the future research and development of SHM are made. Full article

Introduction: Cough is one of the most frequent symptoms reported to pulmonologists. The role of bronchoscopy in the diagnostic work-up of chronic cough is not clearly defined. The aim of this study was to evaluate the utility of fiberoptic bronchoscopy (FOB) and additional testing of samples collected during FOB in the differential diagnosis of chronic cough in adults. Material and methods: This was a single-center retrospective study. Out of 7115 conventional white light FOB examinations, we finally selected 198 with cough as the only indication. Results: In 40.9% of bronchoscopic examinations, no visible cause of cough was found. Visual signs of chronic bronchitis (CB) were detected in 57.6% of reports. Only in 3 cases (1.5%) bronchoscopy revealed a potential cause of chronic cough other than CB. Mycobacterium tuberculosis or other mycobacteria were spotted in none of the samples. In 91.1% of bronchoalveolar lavage (BAL) cytologic examinations, at least one cell count abnormality was detected, but only in case of increased percentage of eosinophils, it might be considered clinically relevant. In 53% of bacteriological culture results, at least one potentially pathogenic bacterium was isolated. Conclusions: The present study results strengthen the evidence that FOB combined with additional testing of airway specimens obtained during FOB is not a powerful tool in the differential diagnosis of chronic cough, and FOB as a diagnostic tool may be overused. The appropriate timing and decision regarding referral for FOB and additional testing of achieved material requires careful clinical consideration. Full article