Search Results (8)

Monitoring the oceans and establishing a global ocean observing system is a task of paramount importance for topics as diverse as the study of climate change, the management of marine environments, and the safety of coastal areas and marine traffic. These systems must be based on long-term observations that allow the correct modeling of the behavior of the seas and the proper environmental management of them. Despite the logical present trend toward automation, in situ measurements from oceanographic vessels are still needed at present, especially when dealing with biogeochemical variables or when seeking information from the subsurface or deep layers of the sea. Long-term measurements by oceanographic vessels can be carried out at one single fixed oceanographic station with a high sampling frequency (typically once a month) or across a grid of stations. In the latter case a larger geographical area is usually covered, but the cost is a reduction of sampling frequency. The question that arises is: what objectives can be achieved, and what questions can be answered according to the sampling frequency and the spatial coverage of the monitoring program? In this work, we analyze the influence of the sampling frequency on the capacity of observing programs to capture the temporal variability of ocean variables at different time scales and to estimate average seasonal cycles and long-term trends. This analysis is conducted through the study of sea surface chlorophyll concentrations in the Western Mediterranean. The trade-off between sampling frequency and spatial coverage is addressed. For this purpose, a monitoring program in the Spanish Mediterranean waters is used as a case study. We show that monthly and fortnightly intervals are the best sampling frequencies for describing the temporal variability of ocean variables as well as their average seasonal cycles. Quarterly sampling could also be appropriate for estimating such seasonal cycles. Surprisingly, the limitations of these low frequency samplings do not arise from the high frequency variability of ocean variables but from the shape of the seasonal cycles. Both high and low frequency sampling designs could be suitable for detecting long-linear trends, depending on the variance of the noise and that of the trend. In the case of quarterly sampling, we show that some statistics improve with the length of the time series, whereas others do not. Although some results may be related to the dynamics of this region, the results are generally applicable to any other marine monitoring system. Full article

This study aimed to determine the dynamics of dormancy using triphenyl tetrazolium chloride (TTC) and asymbiotic germination in Laelia speciosa (Kunth) Schltr. seeds stored for three years and one year at different temperatures. This is the first report of a circannual rhythm in L. speciosa seeds under controlled storage conditions. Two experiments were carried out: (a) with seeds from wild populations of L. speciosa collected at two different times and dehydrated to 4% relative humidity (RH) and stored for three years at 25 °C, 6 °C, −20 °C, and −80 °C, and (b) with seeds from six fruits stored in liquid nitrogen (LN2; −196 °C) at 25 °C for 12 months. The germination conditions were 25 ± 1 °C with 16 h of light (23 μmol m⁻² s⁻¹) and 8 h of darkness for both trials. Because they have a rudimentary embryo, orchids are attributed a morphological latency; however, the staining of the embryo with TTC (>92%) in all the evaluations carried out throughout a year and the decrease in asymbiotic germination in the intermediate evaluations record a circannual biological cycle or clock, under temperature and humidity control (4% RH). Full article

The Instituto Español de Oceanografía (IEO, Spanish Institute of Oceanography) has maintained different monitoring programs in the Spanish Mediterranean waters (Western Mediterranean) since 1992. All these monitoring programs were unified in 2007 under the current program RADMED (series temporales de datos oceanográficos en el Mediterráneo), which is devoted to the in situ multidisciplinary sampling of the water column of coastal and open-sea waters by means of periodic oceanographic campaigns. These campaigns, together with a network of tide-gauges, are part of the IEO Observing system (IEOOS). In some cases, the temperature and salinity time series collected in the frame of these monitoring programs are now more than 30 years long, whereas sea level time series date to the beginning of the 1940s. This information has been complemented with international databases and has been analyzed in numerous works by the Grupo mediterráneo de Cambio Climático (GCC; Mediterranean Climate Change Group) for more than 20 years. These works have been devoted to the detection and quantification of the changes that climate change is producing on the physical, chemical, and biological properties of the Spanish Mediterranean waters. In this work, we review the results obtained by the GCC since 2005 in relation to the changes in the physical properties of the sea: water column temperature, salinity, and density, heat content, mixed layer depth, and sea level. Time series and results are updated from the last works, and the reliability of the existing time series for the detection of climatologies and long-term trends are analyzed. Furthermore, the different sources of uncertainty in the estimation of linear trends are considered in the present work. Besides this review and update of the results obtained from the data collected in the frame of the IEOOS, we conduct a review of the existing monitoring capabilities from other institutions in the Spanish Mediterranean waters and a review of results dealing with climate change in the Spanish Mediterranean obtained by such institutions. In particular, we include a review of the results obtained by SOCIB (Servicio de Observación y Predicción Costero de las Islas Baleares; Balearic Islands costal observing and forecasting system) in relation to the study of marine heat waves and the warming of the sea surface, and the results corresponding to the intense warming of the Catalan continental shelf at L’Estartit oceanographic station. All these results evidence that the surface Spanish Mediterranean waters are warming up at a rate higher than that affecting the global ocean (>2 °C/100 years). This warming and a salinity increase are also observed along the whole water column. Marine heat waves are increasing their intensity, frequency, and duration since 1982, and coastal sea level is increasing at a rate of 2.5 mm/yr. The salinity increase seems to have compensated for the warming, at least at surface and intermediate waters where no significant trends have been detected for the density. This could also be the reason for the lack of significant trends in the evolution of the mixed layer depth. All these results highlight the importance of monitoring the water column and the necessity of maintaining in situ sampling programs, which are essential for the study of changes that are occurring throughout the Spanish Mediterranean waters. Full article

We present an attempt to estimate the long-term changes in Relative Sea Level (RSL), in addition to the different factors contributing to such trends on a local and regional scale, using a statistical linear model. The time series analysis corresponded to 17 tide-gauges, grouped in three different areas: the northern and western Atlantic coasts of the Iberian Peninsula, the Canary Islands, and the southern and eastern coasts of the Iberian Peninsula and Balearic Islands. The analysis was performed for two periods: 1948–2019, using tide-gauge data; and 1993–2019, using both tide-gauge and altimetry data for comparison. The trends for the period 1948–2019 ranged between 1.09 ± 0.14 (Canary Islands) and 2.05 ± 0.21 mm/yr for the northern and western Atlantic Iberian Peninsula. Altimetry data during the period 1993–2019 yielded quite homogeneous results for all the locations and regions, ranging between 2.7 ± 0.4 and 3.0 ± 0.3 mm/yr. In contrast, the results obtained from tide-gauge data for this recent period showed a large dispersion, very likely due to local effects, or perhaps even to levelling or instrumental errors. Nevertheless, when the results were averaged for each area, the observed trends were comparable to the altimetry results, with values of 2.3 ± 0.8, 2.7 ± 0.5, and 2.8 ± 0.8 mm/yr for the three regions of study. A stepwise forward linear regression was used to relate the observed RSL variability to the atmospheric forcing and the thermosteric and halosteric components of the sea level. Surprisingly, the thermosteric and halosteric contributions were not significantly correlated to the observed RSL in many cases; consequently, the steric, the total addition of mass, the mass of salt, and the freshwater contributions to the observed sea level trends could not be reliably estimated. This result seems to have been the consequence of the scarcity of temperature and salinity data; this hypothesis was confirmed, with the exception of the tide-gauge data for L’Estartit. This location is close to a well sampled region. In this case, the atmospheric variables and the thermosteric and halosteric terms accounted for 80% of the observed RSL variance, and the contributions of these terms could be estimated. The freshwater contribution for this location was between 1.3 and 1.4 mm/yr, consistent with recent estimations of the contributions of glaciers and Greenland and Antarctica Ice Sheets. These results highlight the importance of monitoring programs and routine sampling for the determination of the different factors contributing to the sea level variability. Full article

Xenobiotic reductase B (XenB) catalyzes the reduction of the aromatic ring or nitro groups of nitroaromatic compounds with methyl, amino or hydroxyl radicals. This reaction is of biotechnological interest for bioremediation, the reuse of industrial waste or the activation of prodrugs. However, the structural factors that explain the binding of XenB to different substrates are unknown. Molecular dynamics simulations and quantum mechanical calculations were performed to identify the residues involved in the formation and stabilization of the enzyme/substrate complex and to explain the use of different substrates by this enzyme. Our results show that Tyr65 and Tyr335 residues stabilize the ligands through hydrophobic interactions mediated by the aromatic rings of these aminoacids. The higher XenB activity determined with the substrates 1,3,5-trinitrobenzene and 2,4,6-trinitrotoluene is consistent with the lower energy of the highest occupied molecular orbital (LUMO) orbitals and a lower energy of the homo orbital (LUMO), which favors electrophile and nucleophilic activity, respectively. The electrostatic potential maps of these compounds suggest that the bonding requires a large hydrophobic region in the aromatic ring, which is promoted by substituents in ortho and para positions. These results are consistent with experimental data and could be used to propose point mutations that allow this enzyme to process new molecules of biotechnological interest. Full article

One of the effects of climate change is the rise of sea level, which poses an important threat to coastal areas. Therefore, the protection and management of coastal ecosystems as well as human infrastructures and constructions require an accurate knowledge of those changes occurring at a local scale. In this study, long time series of sea level from tide gauges distributed along the southern (Atlantic) and eastern (Mediterranean) Spanish coasts were analyzed. Linear trends were calculated for two periods, from early 1940s to 2018 and from 1990 to 2018. Values for the former period ranged between 0.68 and 1.22 mm/year. These trends experienced a significant increase for the second period, when they ranged between 1.5 and 4.6 mm/year. Previous research analyzed the effect of atmospheric forcing in the Mediterranean Sea by means of 2D numerical models, and the steric contribution was directly evaluated by the integration of density along the water column. In this study, the effect of atmospheric forcing and the thermosteric and halosteric contributions on coastal sea level were empirically determined by means of statistical linear models that established which factors affected sea level at each location and what the numerical response of the observed sea level was to the contributing factors. Atmospheric pressure and the west–east component of the wind hada significant contribution to the sea level variability at most of the tide gauges. The thermosteric and halosteric components of sea level also contributed to the sea level variability at all the tide gauges, with the only exception of Alicante. Atmospheric forcing and the steric components of sea level experienced long-term trends. The combination of such trends, with the response of sea level to these factors, allowed us to estimate their contribution to the observed sea level trends. The part of these trends not explained by the atmospheric variables and the steric contributions was attributed to mass addition. Trends associated with mass addition ranged between 0.6 and 1.2 mm/year for the period 1948–2018 and between 1.0 and 4.5 mm/year for the period 1990–2018. Full article

It is widely accepted that the Mediterranean is an oligotrophic sea where winter mixing favors the proliferation of diatoms and high values of zooplanktonic biomass, mainly associated with the growth of copepods. Stratified conditions from mid-spring to late autumn are dominated by the picophytoplanktonic groups and the increment of cladoceran abundances. This general picture has important exceptions. A regionalization of the Mediterranean Sea can be established, distinguishing oligotrophic and mesotrophic areas and different blooming periods. The RADMED monitoring program covers a large area from the southwestern limit of the Mediterranean to the Catalan Sea. The analysis of phyto and zooplankton time series extending from 1992 to 2016 in some cases, and from 2007 to 2016 in others, have shown that the Spanish Mediterranean waters have differentiated areas and trophic regimes as a result of the existence of several fertilizing mechanisms which include winter mixing, tidal mixing in the Strait of Gibraltar, cyclonic circulation cells and frontal systems. The present work describes these different mechanisms acting on the Spanish Mediterranean waters, and also the potentiality of monitoring programs for providing statistics suitable for operational activities or the initialization/validation of ecological models. Full article

The Cape of Gata region (southeast Spain) allocates the thermo-haline Almeria–Oran front (AOF), which separates two biogeographical zones, with a very irregular bathymetry, consisting of two canyons and seamounts in an area of 100 × 100 km. An interdisciplinary oceanographic sampling strategy allowed us to solve mesoscale processes including current–bathymetry interactions. Subsurface fertilizing processes and elevated chlorophyll a concentrations were found at the front, seamount, and submarine canyons, turning an apparently oligotrophic area into a rich one. According to a horizontal tracking simulation, the deep chlorophyll maximum (DCM) at the front is located above the pycnocline and travels fast offshore, transporting productivity from the fertilization process quickly from the region. The DCM at the seamount, in contrast, develops below the pycnocline and remains for almost three weeks in this area. In spite of the coastal marine protected areas (MPAs), a high surface nitrate concentration plume with its origin in a small coastal area without any protection was detected. Local circulation patterns and bathymetry–current interactions provide elevated productivity in surface water which is vertically connected to deep-sea fauna via the daily vertical migration of zooplankton, suggesting elevated biodiversity on the seamount and canyons of the area studied. Based on these results, and considering the presence of coastal MPAs and a Coastal Area Management Program, future studies on benthic fauna, an enlargement of coastal MPAs, and a transboundary land–deep-sea management program are suggested. Full article