Prospective Association of the Portfolio Diet with All-Cause and Cause-Specific Mortality Risk in the Mr. OS and Ms. OS Study
by
, , and
Kenneth Lo
1,2,*
,
Andrea J. Glenn
3,4,5,
Suey Yeung
6,
Cyril W. C. Kendall
3,4,5,7,
John L. Sievenpiper
3,4,5,8,9,10,
David J. A. Jenkins
3,4,5,8,9,10 and
Jean Woo
6,*
1
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, 11 Yuk Choi Road, Hung Hom, Kowloon, Hong Kong SAR, China
2
Research Institute for Smart Ageing, The Hong Kong Polytechnic University, Hong Kong SAR, China
3
Department of Nutritional Sciences, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada
4
Clinical Nutrition and Risk Factor Modification Center, St. Michael’s Hospital, Toronto, ON M5C 2T2, Canada
5
Toronto 3D Knowledge Synthesis and Clinical Trials Unit, St. Michael’s Hospital, Toronto, ON M5C 2T2, Canada
6
Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
7
College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada
8
Department of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada
9
Li Ka Shing Knowledge Institute, St. Michael’s Hospital, Toronto, ON M5B 1A6, Canada
10
Department of Medicine, Division of Endocrinology and Metabolism, St. Michael’s Hospital, Toronto, ON M5C 2T2, Canada
*
Authors to whom correspondence should be addressed.
Nutrients 2021, 13(12), 4360; https://doi.org/10.3390/nu13124360
Submission received: 3 November 2021
/
Revised: 29 November 2021
/
Accepted: 1 December 2021
/
Published: 3 December 2021
(This article belongs to the Special Issue Associations between Dietary Patterns, Nutrition and Risk of Cancer)
Abstract
:The Portfolio Diet has demonstrated its cardiovascular benefit from interventions, but the association between Portfolio Diet adherence and the risk of all-cause and cause-specific mortality has not been examined in Chinese population. The present study has collected Portfolio Diet adherence (assessed by food frequency questionnaire), lifestyle factors and mortality status of 3991 participants in the Mr. Osteoporosis (OS) and Ms. OS Study. Cox regression models were used to examine the association between the Portfolio Diet adherence and mortality risk (all-cause, cardiovascular disease or cancer). The highest quartile of the Portfolio Diet score was associated with a 28% lower risk of all-cause (hazard ratio, HR: 0.72) and cancer (HR: 0.72) mortality, respectively. The association between Portfolio Diet adherence and cardiovascular disease mortality did not reach statistical significance (HR: 0.90, 95% CI = 0.64, 1.26). Among male participants, the highest adherence to the Portfolio Diet was also associated with a lower risk of all-cause (HR: 0.63) and cancer mortality (HR: 0.59), and there was an inverse association between food sources of plant protein and the risk of cardiovascular mortality (HR: 0.50). However, most associations between the Portfolio Diet and mortality were not significant among females. The protection for cancer mortality risk might reach the plateau at the highest adherence to the Portfolio Diet for females. To conclude, greater adherence to the Portfolio Diet was significantly associated with a lower risk of mortality in Hong Kong older adults, and the associations appeared stronger among males.
1. Introduction
Maintaining a healthy diet is a major lifestyle factor to prevent diabetes and cardiovascular diseases (CVD), two chronic diseases that have resulted in a huge public health burden worldwide. Although multiple studies have evaluated the relationship between individual nutrients and health, emerging evidence has suggested how dietary patterns may explain the interactions between different foods and food components and their associations with health outcomes [1]. Some well-known dietary patterns include the Mediterranean and the Dietary Approaches to Stop Hypertension (DASH) diets [2,3].
Amongst dietary patterns that may improve cardiovascular health, the Dietary Portfolio, or the Portfolio Diet, is a plant-based dietary pattern developed in the early 2000s to lower low-density lipoprotein cholesterol (LDL-C) [4,5]. The Portfolio Diet is low in saturated fat and cholesterol, as in the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III) diet [6], with added plant-based foods or nutrients with cholesterol-lowering properties, namely nuts, plant protein (soy and pulses), viscous fiber sources (oats, barley, psyllium, eggplant, okra, and recently apples, oranges and berries), plant sterols and monounsaturated fats (olive oil, sunflower oil, canola oil, avocado). In 2018, a meta-analysis of randomized controlled trials showed that the Portfolio Diet significantly lowered LDL-C by 17% more than the NCEP ATP III diet alone [7].
In addition to clinical trials for patients with dyslipidemia, the health benefits of the Portfolio Diet were also examined among cohort studies. A Portfolio Diet score (PDS) has been developed and validated to assess the adherence to the Portfolio Diet using food frequency questionnaires (FFQ) [8], and to date, the PDS has been associated with lower cardiometabolic risk factors in the Prevención con Dieta Mediterránea (PREDIMED)-Plus cohort and lower risk of CVD in the Women’s Health Initiative (WHI) [9,10].
Despite groundwork being established, the PDS needs to be assessed in other cohorts and ethnic groups to compare with previous findings and verify its applicability internationally. In addition, the previous work using WHI data only included postmenopausal women. The present study has evaluated how the adherence to the Portfolio Diet may have sex-specific associations with the risk of all-cause, CVD and cancer mortality among a prospective cohort of Hong Kong older adults, an ageing Chinese population with high life expectancy [11]. When analyzing the secular trends of cancer incidences in 1983–2017, the total incident cancer case counts increased 156.5% for women and 96% for men, which might be due to population growth (66.1% for women, 25.4% for men) and population ageing (95% for women, 119.4% for men) [11]. That would be important to evaluate how a healthy dietary pattern may reduce the mortality risk due to chronic diseases for older adults.
2. Materials and Methods
2.1. Study Population
To measure the Portfolio Diet adherence in the Hong Kong Chinese context, we have used data from the Mr. Osteoporosis and Ms. Osteoporosis Study (OS Study, hereafter). Since 2001–2003, the OS Study has been a long-term cohort study that recruited 4000 community-living Hong Kong Chinese men and women aged at least 65 years. The target was to recruit a stratified sample that equally assigned them into each of these age groups, namely 65–69, 70–74, and ≥75. Compared with the general population in this age group, participants had higher educational level (9.8% vs. 3.8% with tertiary education), higher proportion of being married (70.7% vs. 59.9%) and slightly lower proportion of living alone (10.8% vs. 11.3%) [12]. The study was performed based on the guidelines set in the Declaration of Helsinki and was approved by the Clinical Research Ethics Committee of The Chinese University of Hong Kong. Written informed consent was obtained from all participants. Dietary assessment has been administered as an FFQ to all participants at baseline, and the data on mortality statistics has been obtained from the Death Registry collected through March 2014 [12].
2.2. Dietary Assessment
Dietary assessment was conducted using a validated FFQ (OS FFQ, hereafter) with 280 food items validated against 24 dietary recalls [13,14]. Each participant was asked to respond to the FFQ by trained personnel by answering how often they consumed each food item each day or each week, and the size of each portion, over the past year. A pictorial catalogue with individual food portions was provided as a guide.
Food items recommended in the Portfolio Diet were extracted from the OS FFQ and were categorized into the six components of the Portfolio Diet. For most components, servings per day were summed over all consumed food items in each component for every participant. More points were given to participants with higher intakes of food recommended in the Portfolio Diet, whereas fewer points were given to participants with higher intakes of food not recommended in the Portfolio Diet. These points were given for each of the six dietary components (plant protein sources, nuts, viscous fiber, plant sterols, monounsaturated fatty acids (MUFA), saturated fat/cholesterol sources) by splitting the components into quintiles (according to the sex-specific data distribution in the OS Study). Those in the highest quintile of foods recommended (e.g., nuts) received 5 points and those in the lowest quintile received 1 point. Reverse scoring was done for those with foods not recommended (e.g., foods high in saturated fat), meaning those with the highest intake (quintile 5) received 1 point, and those with the lowest intake (quintile 1) received 5 points. The total points were then added for each respondent, resulting in a score range between 6 and 30, with higher scores indicating higher adherence to the Portfolio Diet. The adherence score of Portfolio Diet has been validated in 652 participants in the Toronto Healthy Diet Study, which has shown inverse association with lower LDL-C, and showed reasonable agreement between the score from the FFQ and 7-day dietary record [8].
Plant sterols were the only score component based on mg per day, while all other components are food-based in servings per day. Since the OS FFQ compositional database does not have plant sterols available as a nutrient variable for their FFQ, we have established a plant sterol database (mg per day) based on literature values. Data sources in European countries or the United States were used, including the Finnish Food Composition Database [15], the database used in the European Prospective Cohort into Cancer cohort [16], and the United States Department of Agriculture [17]. For food items specific to the Asian context, we obtained the nutritional values from food composition databases or academic publications [18,19,20]. We created recipes and determined plant sterol values for foods in the OS FFQ that does not have a plant sterol value available in the literature, using the ESHA Research Food Processor SQL: Nutrition Analysis and Fitness Program (Copyright 2012, ESHA Research).
2.3. Ascertainment of Mortality Outcomes
Data on mortality were obtained from the Death Registry of the Department of Health of Hong Kong and collected through March 2014 with a median follow-up of 11.1 years. Apart from all-cause mortality, cardiovascular or cancer causes of death were identified by the cause of death reported on the death certificate and classified according to the International Classification of Disease version 10 codes. Participants were followed up until death or loss to follow-up, whichever occurred first [13].
2.4. Covariates
Demographic, lifestyle and health information was collected from each participant including education level (secondary school or below vs. post-secondary education), smoking habit (never, former or current smoker), alcohol consumption (drink > 12 alcohol drinks in the previous year), physical activity, dietary intake and self-reported history of chronic diseases (diabetes, hypertension, stroke, heart attack, angina, congestive heart failure or cancer). Physical activity level was evaluated using the Physical Activity Scale for the Elderly (PASE) [21]. Body weight was measured using the Physician Balance Beam Scale (Healthometer, IL, USA) to the nearest 0.1 kg with participants wearing a light gown. Height was measured to the nearest 0.1 cm using the Holtain Harpenden Standiometer (Holtain Ltd., Crosswell, UK), which were used to compute body mass index (BMI).
2.5. Statistical Analysis
Baseline characteristics were stratified by sex (male or female) and the quartiles of the PDS using means with standard deviations for continuous variables and frequencies with percentages for categorical variables. To compare baseline characteristics, chi-square tests were used for categorical variables and analysis of variance for continuous variables. Participants were categorized into quartiles of the Portfolio Diet score, with the lowest quartile serving as the reference group. Cox proportional hazard models were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) for the association between the PDS quartiles and mortality outcomes. The Cox regression model was adjusted for sex (male vs. female), age, dietary energy, body mass index, physical activity, systolic blood pressure (all continuous variables), medical history (yes vs. no for diabetes, hypertension, stroke, heart attack, angina, congestive heart failure or cancer), smoking habit (never, former or current smokers), alcohol drinking (yes vs. no for having >12 alcoholic drinks in the past year) and education level (post-secondary education vs. secondary school or below). We also applied subgroup analyses according to sex (male or female). Trend analysis was performed by assigning median values of each PDS quartile and treating it as a continuous variable in the regression model. To examine the potential non-linear relationship between the PDS and the risk of mortality, we compared the fit of continuous models with or without cubic spline terms of PDS. A likelihood test with p < 0.05 would suggest a better fit regression model by including the quadratic term, hence a non-linear relationship between the PDS and all-cause, CVD or cancer mortality.
Additional analyses included evaluating associations between the six individual components of the Portfolio Diet and risk of the mortality outcomes. We conducted subgroup analysis on the Portfolio Diet components using the same covariates as listed in the regression analysis. Sensitivity analyses on the association between the PDS and the risk of mortality were conducted by excluding people who had ever smoked or with individual comorbidity at baseline (diabetes, hypertension, stroke, heart attack, angina, congestive heart failure, cancer, or any one of the above) as they may have different diets than those without smoking nor comorbidities. Since the influence of the PDS on blood pressure could be the causal pathway on the risk of mortality, we also removed systolic blood pressure from the regression model in a sensitivity analysis. Another sensitivity analysis comprised using the age at death as the survival time in the Cox regression model, instead of the observation period from enrolment to the end of the study or the subject’s death, because the exposure (Portfolio Diet adherence) for each subject began before the period of enrolment. With regard to the competing nature of cardiovascular disease and cancer in mortality risk, we have also computed the cumulative sub-distribution hazards (SHR) for the Fine–Gray models [22], and compared the magnitude of association with that obtained from the standard Cox models. The sex-specific differences in cumulative incidence of mortality due to CVD or cancer were compared using the approaches proposed by Gray [23]. Statistical tests were two-sided and p < 0.05 was considered statistically significant. Most statistical analyses were conducted with IBM SPSS Statistics 23 (IBM Corporation, Armonk, NY) and we have used R 4.1.1 (R Foundation for Statistical Computing, Vienna, Austria) for examining the non-linear relationship between the PDS and the risk of mortality, and the competing risk analysis [22].
3. Results
3.1. Characteristics of OS Study Participants
After excluding 9 people with missing information on dietary intake or covariates, a total of 3991 participants were included in the analyses. The baseline characteristics of participants in the OS Study are presented by sex in Table 1. As shown by independent t-test, male participants tended to have, on average, higher dietary energy, lower BMI, higher physical activity and lower SBP compared to female participants. Male participants also had higher consumption in food sources rich in plant protein, nuts, plant sterols and saturated fat/cholesterol, but lower consumption in food sources rich in viscous fiber and MUFAs. As demonstrated by the chi-square test, males were more prevalent in stroke, angina, being a smoker or alcohol drinker, and having post-secondary education compared to females. Supplementary Table S1 provides the details on the mean intake of each Portfolio Diet components by sex-specific quintiles.
The baseline characteristics of participants in the OS Study by PDS quartiles are presented in Table 2. As shown by one-way ANOVA, participants in the highest quartile of PDS were, on average, more likely to have post-secondary education, be younger in age, have a higher physical activity level and higher intake of energy, and lower smoking rate. but the rate of co-morbidities was similar across PDS quartiles. We have also compared the sex-specific cumulative incidence of mortality due to CVD or cancer in Supplementary Figure S1, and did not find significant differences in incidence by sex (both p > 0.05).
3.2. Portfolio Diet Score and Mortality Outcomes
The association between the Portfolio Diet adherence in quartiles and the risk of mortality are presented in Table 3. When compared to participants with the lowest adherence, the highest adherence to the Portfolio Diet was associated with a lower risk of all-cause mortality (HR: 0.72, 95% CI = 0.62, 0.86) and cancer mortality (HR: 0.72, 95% CI = 0.54, 0.96). The association between the PDS and CVD mortality did not reach statistical significance (HR: 0.90, 95% CI = 0.64, 1.26). Among male participants (Table 3), the highest adherence to the Portfolio Diet was also associated with a lower risk of all-cause mortality (HR: 0.63, 95% CI = 0.51, 0.79) and cancer mortality (HR: 0.59, 95% CI = 0.39, 0.87). Among females, participants in the second (HR: 0.65, 95% CI = 0.43, 0.98) and third (HR: 0.62, 95% CI = 0.40, 0.95) quartiles of PDS had associations with a lower risk of cancer mortality. The p-values for the trend were significant for the association between PDS and all-cause mortality among all participants and males only, as well as the association between PDS and cancer mortality among overall participants. The p-values for non-linearity were significant for the association between PDS and all-cause mortality among males, and the association between PDS and cancer mortality among males and females. We then checked the assumption by testing the proportionality of the risks according to outcomes (all-cause, CVD or cancer mortality) and sex (male, female). As revealed by the Schoenfeld test in Supplementary Figure S2, most Cox regression models did not violate the assumption except for the association between PDS and all-cause mortality among all participants (p = 0.0199).
When looking into individual components of the Portfolio Diet (Table 4), higher consumption of plant protein sources (HR: 0.77, 95% CI = 0.62, 0.95), viscous fiber sources (HR: 0.78, 95% CI = 0.62, 0.98) and nuts (HR: 0.73, 95% CI = 0.58, 0.91) were associated with a lower risk of mortality among males. There was also an inverse association between food sources of plant protein and the risk of cardiovascular mortality (HR: 0.50, 95% CI = 0.30, 0.81) among male participants. However, associations between adherence to the Portfolio Diet score and each mortality outcome were not significant among females.
3.3. Sensitivity Analysis and Competing Risk Analysis
After excluding participants who smoked or with comorbidities at baseline, excluding SBP from the regression model, or using the age at death as survival time, the association between the highest quartile of PDS and the risk of mortality remained significant among male participants (Supplementary Table S2). Among female participants, the association between the highest quartile of PDS and the risk of mortality remained insignificant in most sensitivity analyses, except the significant association between PDS, all-cause (HR: 0.62, 95% CI = 0.42, 0.92) and cancer mortality (HR: 0.52, 95% CI = 0.27, 0.98) after excluding participants with hypertension (Supplementary Table S2). The association between PDS and CVD mortality was insignificant in all sensitivity analyses regardless of sex (Supplementary Table S2). As demonstrated from competing risk analysis on the association between PDS and the risk of mortality due to CVD and cancer (Supplementary Table S3), the association between the PDS and CVD mortality did not reach statistical significance regardless of sex. Among male participants, the third (SHR: 0.88, 95% CI = 0.52, 0.93) and the highest (SHR: 0.54, 95% CI = 0.43, 0.67) adherence to the Portfolio Diet associated with a lower risk of cancer mortality. Among females, participants in the second (SHR: 0.64, 95% CI = 0.42, 0.97) and third (SHR: 0.60, 95% CI = 0.39, 0.93) quartiles of PDS had associations with a lower risk of cancer mortality. The magnitude of association did not have a substantial difference when compared to the results obtained from standard Cox regression model (Table 3).
4. Discussion
4.1. Summary of Findings
This examination of approximately 4000 older adults in Hong Kong is the first prospective cohort study to examine the association between the Portfolio Diet and mortality in the Asian region. A higher Portfolio Diet score was associated with a 28% lower risk of all-cause and cancer mortality, respectively. Among male participants, the highest adherence to the Portfolio Diet was also associated with a lower risk of all-cause and cancer mortality, and there was an inverse association between food sources of plant protein and the risk of cardiovascular mortality. Since most associations were not significant among female participants, the findings from the present study are likely driven by male participants. Sex differences in the consumption of food sources rich in plant protein, viscous fiber and nuts, as well as smoking rate, may explain the relationship between the Portfolio Diet and mortality risk reduction. Despite some non-linearity in the shape of the relationships, the general results from the present study have suggested protective associations of the Portfolio Diet in decreasing the risk of mortality. The protection for all-cause and cancer mortality risk may be significant at the highest quartile of PDS for males, while the protection for cancer mortality risk might reach the plateau at the highest quartile of PDS for females.
4.2. Comparison with Previous Literature
To the best of our knowledge, this study is the first to demonstrate the potential role of the Portfolio Diet in preventing all-cause mortality; therefore, comparison with other literature is challenging. An analysis of postmenopausal women that participated in the WHI showed that the adherence to the Portfolio Diet was associated with lower risk of total CVD (HR: 0.89, 95% CI: 0.83, 0.94), coronary heart disease (HR: 0.86, 95% CI: 0.78, 0.95), and heart failure (HR: 0.83, 95% CI: 0.71, 0.99), comparing the highest to lowest quartile of adherence [10], which is not in line with the findings from our study. In other words, the relatively small sample size (3991 older adults when compared with 123,330 postmenopausal women in WHI) for the present study might limit the statistical power to detect associations between PDS and CVD mortality. Future studies should verify the long-term association between PDS and CVD outcomes in larger prospective cohorts.
Although the present study has not demonstrated the long-term benefit of the Portfolio Diet in reducing mortality risk due to CVD, further recent analyses revealed the prospective benefit of the adherence to the Portfolio Diet on cardiometabolic health. Higher adherence to the PDS was associated with lower LDL-C in the Toronto Healthy Diet Study [8], as well as with improvements in cardiometabolic risk factors, including glycated hemoglobin, fasting plasma glucose, triglycerides, waist circumference and BMI, in the PREDIMED-Plus cohort [9].
When looking into the individual components of the Portfolio Diet, plant protein, nuts and viscous fiber was associated with lower mortality risk, whereas plant protein consumption was associated with lower risk of CVD death in our analysis. In the present analysis, the magnitude of association between the Portfolio Diet and all-cause mortality (HR: 0.72, 95% CI = 0.61, 0.86) was comparable to plant protein sources (HR: 0.77, 95% CI = 0.62, 0.95), viscous fiber sources (HR: 0.78, 95% CI = 0.62, 0.98) and nuts (HR: 0.73, 95% CI = 0.58, 0.91). Moreover, plant protein sources had an inverse association with CVD mortality among male (HR: 0.50, 95% CI = 0.30, 0.81) participants. The findings of our present analyses are in line with related studies on the association between plant-based foods, CVD and cancer mortality. For example, evidence from meta-analyses have suggested the consumption of legumes [24], viscous fiber sources [25], and nuts [26] were associated with a lower risk of CVD events. A recent meta-analysis of cohort studies also suggests that higher plant protein intake is associated with a reduced risk of all-cause and CVD-related mortality, while the association was not significant for cancer mortality regardless of sex [27]. However, it is notable that only 2 out of 15 analyses on plant protein and cancer mortality were conducted in China [27]. The geographical variation in diet is likely to be overlooked, and therefore more prospective cohorts should be conducted in Asian regions.
Our subgroup analyses showed that greater adherence to the Portfolio Diet was associated with a lower risk of all-cause and cancer mortality among men only. Similarly, an analysis of the Adventist Health Study 2 found that vegetarian dietary patterns (a combined category of vegan, lacto-ovo–vegetarian, pesco-vegetarian, and semi-vegetarian) were associated with a reduced risk of mortality due to ischemic heart disease or CVD in men but the associations were insignificant in women [28]. Although authors did not investigate the reasons behind this finding, they postulate that the nutrient profile of the dietary patterns might differ by sex [28]. Meanwhile, in our study, the sex-specific adherence to individual Portfolio Diet components might have attributed to the differential associations between PDS and mortality. When compared to female participants, men consumed more food sources of plant protein, nuts, plant sterols and saturated fat/cholesterol, although only plant protein has demonstrated prospective associations with the risk of all-cause and CVD mortality among men. Our findings may suggest that a better adherence to the Portfolio Diet recommendation, e.g., 21.4 g of soy protein per 1000 kcal, can be translated into long-term health benefit [29]. Moreover, men were also more likely to have evidence of stroke and angina at baseline, but the sensitivity analyses suggested that both comorbidities did not have substantial influence on the magnitude of the association. When compared to WHI population [10], the female participants in the present study had a higher consumption of food sources rich in plant protein (9.07 vs. 0.77 servings/day at the highest quintile of intake), which may be attributed to the intake of soy and soy products [30]. Further research is necessary to verify the potential sex-specific associations between PDS and cause-specific mortality and the mechanisms behind them, especially in Asian regions.
4.3. Strengths and Limitations
The strengths of our study include the prospective cohort design and over 10 years of follow-up for mortality outcomes. Nevertheless, there are some limitations. First, participants in the OS Study had a higher educational level compared with the general Hong Kong population and, therefore, they may have better dietary habits and improved dietary profile. Second, although the FFQ is considered a common method to collect data of usual dietary intake in large epidemiological studies, the data are self-reported. In addition, the smaller number of CVD mortality events than all-cause and cancer deaths may explain the null association between Portfolio Diet adherence and the risk of CVD mortality. Moreover, diet and lifestyle factors might change over time, and the relevant information was only available at baseline in this current study. Lastly, causation cannot be established because of the observational design, and residual confounding cannot be ruled out.
5. Conclusions
Greater adherence to a Portfolio Diet was significantly associated with lower risk of mortality in Hong Kong older adults. These findings have supported the benefits of a Portfolio Diet in reducing the risk of cancer mortality. However, our findings have to be verified in additional populations, including in cohort studies that measured repeated dietary habits over time.
Supplementary Materials
The following are available online at https://www.mdpi.com/article/10.3390/nu13124360/s1, Supplementary Figure S1: Cumulative probability of mortality due to cardiovascular disease (CVD) or cancer, Supplementary Figure S2: Testing proportional Hazards assumption, Supplementary Table S1: Comparing the Portfolio Diet score components in male and female participants, Supplementary Table S2: Sensitivity analysis on the associations between the highest to lowest adherence to the Portfolio Diet and risk of mortality, Supplementary Table S3: Competing risk analysis on the association between Portfolio Diet Score and the Risk of CVD or Cancer Mortality.
Author Contributions
Conceptualization, K.L., A.J.G., C.W.C.K., J.L.S. and D.J.A.J.; Data curation, K.L., A.J.G., S.Y. and J.W.; Formal analysis, K.L. and A.J.G.; Funding acquisition, K.L.; Investigation, S.Y. and J.W.; Writing–review and editing, K.L., A.J.G., S.Y., C.W.C.K., J.L.S., D.J.A.J. and J.W. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. All authors have read and agreed to the published version of the manuscript.
Funding
K.L. received funding from the Funding for Research Institutes (Grant number: CD69) and Start-up Fund for RAPs under the Strategic Hiring Scheme (Grant number: BD8H). A.J.G. received funding from the Nora Martin Fellowship in Nutritional Sciences, Banting & Best Diabetes Centre Tamarack Graduate Award in Diabetes Research, the Peterborough K.M. Hunter Charitable Foundation Graduate Award, and the Ontario Graduate Scholarship. J.L.S. was funded by a PSI Graham Farquharson Knowledge Translation Fellowship, Diabetes Canada Clinician Scientist Award, CIHR INMD/CNS New Investigator Partnership Prize, and Banting & Best Diabetes Centre Sun Life Financial New Investigator Award. D.J.A.J. was funded by the Government of Canada through the Canada Research Chair Endowment.
Institutional Review Board Statement
The Mr. OS and Ms. OS study was conducted according to the guidelines laid down in the Declaration of Helsinki, and all procedures involving human subjects were approved by the Clinical Research Ethics Committee of the Chinese University of Hong Kong (CRE: 2003.102).
Informed Consent Statement
Written informed consent was obtained from all participants.
Data Availability Statement
The data presented in this study are available on request from the corresponding author.
Acknowledgments
We thank all Mr. OS and Ms. OS Study participants for their contribution in the study. We also thank Lee Man Yin for his literature search at the early stage of this study, which is instrumental for the dietary score development.
Conflicts of Interest
A.J.G. has received consulting fees from Solo GI Nutrition and has received an honorarium from the Soy Nutrition Institute. C.W.C.K. has received grants or research support from the Advanced Food Materials Network, Agriculture and Agri-Foods Canada (AAFC), Almond Board of California, Barilla, Canadian Institutes of Health Research (CIHR), Canola Council of Canada, International Nut and Dried Fruit Council, International Tree Nut Council Research and Education Foundation, Loblaw Brands Ltd., the Peanut Institute, Pulse Canada and Unilever. He has received in-kind research support from the Almond Board of California, Barilla, California Walnut Commission, Kellogg Canada, Loblaw Companies, Nutrartis, Quaker (PepsiCo), the Peanut Institute, Primo, Unico, Unilever, WhiteWave Foods/Danone. He has received travel support and/or honoraria from the Barilla, California Walnut Commission, Canola Council of Canada, General Mills, International Nut and Dried Fruit Council, International Pasta Organization, Lantmannen, Loblaw Brands Ltd., Nutrition Foundation of Italy, Oldways Preservation Trust, Paramount Farms, the Peanut Institute, Pulse Canada, Sun-Maid, Tate & Lyle, Unilever and White Wave Foods/Danone. He has served on the scientific advisory board for the International Tree Nut Council, International Pasta Organization, McCormick Science Institute and Oldways Preservation Trust. He is a founding member of the International Carbohydrate Quality Consortium (ICQC), Executive Board Member of the Diabetes and Nutrition Study Group (DNSG) of the European Association for the Study of Diabetes (EASD), is on the Clinical Practice Guidelines Expert Committee for Nutrition Therapy of the EASD and is a Director of the Toronto 3D Knowledge Synthesis and Clinical Trials foundation. J.L.S. has received research support from the Canadian Foundation for Innovation, Ontario Research Fund, Province of Ontario Ministry of Research and Innovation and Science, Canadian Institutes of health Research (CIHR), Diabetes Canada, PSI Foundation, Banting and Best Diabetes Centre (BBDC), American Society for Nutrition (ASN), INC International Nut and Dried Fruit Council Foundation, National Dried Fruit Trade Association, National Honey Board (the U.S. Department of Agriculture [USDA] honey “Checkoff” program), Institute for the Advancement of Food and Nutrition Sciences (IAFNS; formerly ILSI North America), Pulse Canada, Quaker Oats Center of Excellence, The United Soybean Board (the USDA soy “Checkoff” program), The Tate and Lyle Nutritional Research Fund at the University of Toronto, The Glycemic Control and Cardiovascular Disease in Type 2 Diabetes Fund at the University of Toronto (a fund established by the Alberta Pulse Growers), The Plant Protein Fund at the University of Toronto (a fund which has received contributions from IFF), and The Nutrition Trialists Fund at the University of Toronto (a fund established by an inaugural donation from the Calorie Control Council). He has received in-kind food donations to support a randomized controlled trial from the Almond Board of California, California Walnut Commission, Peanut Institute, Barilla, Unilever/Upfield, Unico/Primo, Loblaw Companies, Quaker, Kellogg Canada, WhiteWave Foods/Danone, Nutrartis, Dairy Farmers of Canada, and Soylent. He has received travel support, speaker fees and/or honoraria from Diabetes Canada, Dairy Farmers of Canada, FoodMinds LLC, International Sweeteners Association, Nestlé, Pulse Canada, Canadian Society for Endocrinology and Metabolism (CSEM), GI Foundation, Abbott, General Mills, Biofortis, ASN, Northern Ontario School of Medicine, INC Nutrition Research & Education Foundation, European Food Safety Authority (EFSA), Comité Européen des Fabricants de Sucre (CEFS), Nutrition Communications, International Food Information Council (IFIC), Calorie Control Council, International Glutamate Technical Committee, and Physicians Committee for Responsible Medicine. He has or has had ad hoc consulting arrangements with Perkins Coie LLP, Tate & Lyle, Wirtschaftliche Vereinigung Zucker e.V., Danone, and Inquis Clinical Research. He is a member of the European Fruit Juice Association Scientific Expert Panel and former member of the Soy Nutrition Institute (SNI) Scientific Advisory Committee. He is on the Clinical Practice Guidelines Expert Committees of Diabetes Canada, European Association for the study of Diabetes (EASD), Canadian Cardiovascular Society (CCS), and Obesity Canada/Canadian Association of Bariatric Physicians and Surgeons. He serves or has served as an unpaid scientific advisor for the Food, Nutrition, and Safety Program (FNSP) and the Technical Committee on Carbohydrates of ILSI North America. He is a member of the International Carbohydrate Quality Consortium (ICQC), Executive Board Member of the Diabetes and Nutrition Study Group (DNSG) of the EASD, and Director of the Toronto 3D Knowledge Synthesis and Clinical Trials foundation. His wife is an employee of AB InBev. D.J.A.J. has received research grants from Saskatchewan & Alberta Pulse Growers Associations, the Agricultural Bioproducts Innovation Program through the Pulse Research Network, the Advanced Foods and Material Network, Loblaw Companies Ltd., Unilever Canada and Netherlands, Barilla, the Almond Board of California, Agriculture and Agri-food Canada, Pulse Canada, Kellogg’s Company, Canada, Quaker Oats, Canada, Procter & Gamble Technical Centre Ltd., Bayer Consumer Care, Springfield, NJ, Pepsi/Quaker, International Nut & Dried Fruit Council (INC), Soy Foods Association of North America, the Coca-Cola Company (investigator initiated, unrestricted grant), Solae, Haine Celestial, the Sanitarium Company, Orafti, the International Tree Nut Council Nutrition Research and Education Foundation, the Peanut Institute, Soy Nutrition Institute (SNI), the Canola and Flax Councils of Canada, the Calorie Control Council, the Canadian Institutes of Health Research (CIHR), the Canada Foundation for Innovation (CFI) and the Ontario Research Fund (ORF). He has received in-kind supplies for trials as a research support from the Almond board of California, Walnut Council of California, the Peanut Institute, Barilla, Unilever, Unico, Primo, Loblaw Companies, Quaker (Pepsico), Pristine Gourmet, Bunge Limited, Kellogg Canada, WhiteWave Foods. He has been on the speaker’s panel, served on the scientific advisory board and/or received travel support and/or honoraria from Nutritional Fundamentals for Health (NFH)-Nutramedica, Saint Barnabas Medical Center, The University of Chicago, 2020 China Glycemic Index (GI) International Conference, Atlantic Pain Conference, Academy of Life Long Learning, the Almond Board of California, Canadian Agriculture Policy Institute, Loblaw Companies Ltd., the Griffin Hospital (for the development of the NuVal scoring system), the Coca-Cola Company, Epicure, Danone, Diet Quality Photo Navigation (DQPN), Better Therapeutics (FareWell), Verywell, True Health Initiative (THI), Heali AI Corp, Institute of Food Technologists (IFT), Soy Nutrition Institute (SNI), Herbalife Nutrition Institute (HNI), Saskatchewan & Alberta Pulse Growers Associations, Sanitarium Company, Orafti, the International Tree Nut Council Nutrition Research and Education Foundation, the Peanut Institute, Herbalife International, Pacific Health Laboratories, Barilla, Metagenics, Bayer Consumer Care, Unilever Canada and Netherlands, Solae, Kellogg, Quaker Oats, Procter & Gamble, Abbott Laboratories, Dean Foods, the California Strawberry Commission, Haine Celestial, PepsiCo, the Alpro Foundation, Pioneer Hi-Bred International, DuPont Nutrition and Health, Spherix Consulting and WhiteWave Foods, the Advanced Foods and Material Network, the Canola and Flax Councils of Canada, Agri-Culture and Agri-Food Canada, the Canadian Agri-Food Policy Institute, Pulse Canada, the Soy Foods Association of North America, the Nutrition Foundation of Italy (NFI), Nutra-Source Diagnostics, the McDougall Program, the Toronto Knowledge Translation Group (St. Michael’s Hospital), the Canadian College of Naturopathic Medicine, The Hospital for Sick Children, the Canadian Nutrition Society (CNS), the American Society of Nutrition (ASN), Arizona State University, Paolo Sorbini Foundation and the Institute of Nutrition, Metabolism and Diabetes. He received an honorarium from the United States Department of Agriculture to present the 2013 W.O. Atwater Memorial Lecture. He received the 2013 Award for Excellence in Research from the International Nut and Dried Fruit Council. He received funding and travel support from the Canadian Society of Endocrinology and Metabolism to produce mini cases for the Canadian Diabetes Association (CDA). He is a member of the International Carbohydrate Quality Consortium (ICQC). His wife, Alexandra L Jenkins, is a director and partner of INQUIS Clinical Research for the Food Industry, his 2 daughters, Wendy Jenkins and Amy Jenkins, have published a vegetarian book that promotes the use of the foods described here, The Portfolio Diet for Cardiovascular Risk Reduction (Academic Press/Elsevier 2020 ISBN:978-0-12-810510-8)and his sister, Caroline Brydson, received funding through a grant from the St. Michael’s Hospital Foundation to develop a cookbook for one of his studies. He is also a vegan. All other authors declare no conflict.
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Table 1.
Baseline characteristics of participants in Mr. Osteoporosis (OS) and Ms. OS Study.
| Male (n = 1996) | Female (n = 1995) | p Value | |
|---|---|---|---|
| Mean (SD)/n (%) | |||
| Age | 72.39 ± 5.01 | 72.59 ± 5.36 | 0.23 |
| Post-secondary Education | 286 (14.3%) | 130 (6.5%) | <0.01 |
| Physical activity (PASE score) | 97.37 ± 50.29 | 85.29 ± 33.12 | <0.01 |
| Smoking habit | <0.01 | ||
| ● Former smoker | 1036 (51.9%) | 153 (7.7%) | |
| ● Current smoker | 237 (11.9%) | 37 (1.9%) | |
| Drink > 12 alcoholic drinks in the past year | 471 (23.6%) | 51 (2.6%) | <0.01 |
| Dietary energy (kcal) | 2099.09 ± 586.70 | 1582.87 ± 461.70 | <0.01 |
| Plant protein sources (serving) | 5.19 ± 4.42 | 4.03 ± 3.54 | <0.01 |
| Viscous fiber sources (serving) | 3.61 ± 3.41 | 4.25 ± 2.86 | <0.01 |
| Nuts (serving) | 0.09 ± 0.18 | 0.06 ± 0.16 | <0.01 |
| Plant sterols (mg) | 361.50 ± 160.51 | 320.56 ± 168.87 | <0.01 |
| MUFAs sources (serving) | 0.07 ± 0.18 | 0.08 ± 0.17 | <0.01 |
| Saturated fat/cholesterol sources (serving) | 2.26 ± 1.63 | 1.27 ± 1.10 | <0.01 |
| Systolic blood pressure (mmHg) | 141.85 ± 19.81 | 143.41 ± 18.36 | 0.01 |
| Body mass index (kg/m2) | 23.45 ± 3.13 | 23.92 ± 3.45 | <0.01 |
| History of diabetes | 293 (14.7%) | 286 (14.3%) | 0.76 |
| History of hypertension | 834 (41.8%) | 869 (43.6%) | 0.26 |
| History of stroke | 108 (5.4%) | 65 (3.3%) | <0.01 |
| History of heart attack | 200 (10.0%) | 192 (9.6%) | 0.67 |
| History of angina | 205 (10.3%) | 147 (7.4%) | <0.01 |
| History of congestive heart failure | 73 (3.7%) | 78 (3.9%) | 0.68 |
| History of cancer | 87 (4.4%) | 89 (4.5%) | 0.88 |
Chi-square (categorical variables) and one-way ANOVA (continuous variables) for subgroup differences. Abbreviations: PASE: Physical Activity Scale for the Elderly; MUFA: Monounsaturated fatty acids; SD: standard deviation.
Table 2.
Baseline characteristics of participants in Mr. OS and Ms. OS Study by quartiles of Portfolio Diet scores.
Table 2.
Baseline characteristics of participants in Mr. OS and Ms. OS Study by quartiles of Portfolio Diet scores.
| Mean (SD)/n (%) | |||||
|---|---|---|---|---|---|
| Q1 (<14, n = 1168) | Q2 (14–16, n = 944) | Q3 (17–19, n = 1080) | Q4 (≥20, n = 799) | p Value | |
| Age | 72.76 ± 5.36 | 72.69 ± 5.08 | 72.39 ± 5.20 | 71.99 ± 5.02 | <0.01 |
| Male | 548 (46.9%) | 539 (57.1%) | 504 (46.7%) | 404 (50.6%) | 0.01 |
| Post-secondary Education | 87 (7.4%) | 79 (8.4%) | 123 (11.4%) | 127 (15.9%) | <0.01 |
| Physical activity (PASE score) | 86.31 ± 39.83 | 90.22 ± 42.63 | 94.02 ± 44.84 | 96.34 ± 44.57 | <0.01 |
| Smoking habit | <0.01 | ||||
| ● Former smoker | 378 (32.4%) | 246 (26.1%) | 340 (31.5%) | 225 (28.2%) | |
| ● Current smoker | 123 (10.5%) | 69 (7.3%) | 62 (5.7%) | 20 (2.5%) | |
| Drink > 12 alcoholic drinks in the past year | 165 (14.1%) | 121 (12.8%) | 151 (14.0%) | 85 (10.6%) | 0.11 |
| Dietary energy (kcal) | 1682.50 ± 518.81 | 1767.53 ± 574.63 | 1932.87 ± 587.90 | 2035.57 ± 618.66 | <0.01 |
| Plant protein sources (serving) | 2.83 ± 2.08 | 4.19 ± 3.22 | 5.23 ± 3.62 | 6.90 ± 5.92 | <0.01 |
| Viscous fiber sources (serving) | 2.29 ± 1.85 | 3.55 ± 3.23 | 4.52 ± 2.75 | 5.98 ± 3.68 | <0.01 |
| Nuts (serving) | 0.03 ± 0.07 | 0.06 ± 0.10 | 0.09 ± 0.14 | 0.16 ± 0.30 | <0.01 |
| Plant sterols (mg) | 231.05 ± 95.01 | 314.37 ± 135.89 | 390.92 ± 158.44 | 465.88 ± 176.85 | <0.01 |
| MUFAs sources (serving) | 0.01 ± 0.03 | 0.03 ± 0.10 | 0.08 ± 0.16 | 0.24 ± 0.26 | <0.01 |
| Saturated fat/cholesterol sources (serving) | 1.92 ± 1.49 | 1.70 ± 1.50 | 1.60 ± 1.37 | 1.77 ± 1.47 | <0.01 |
| Systolic blood pressure (mmHg) | 142.68 ± 18.78 | 142.82 ± 19.08 | 142.91 ± 19.82 | 141.97 ± 19.11 | 0.73 |
| Body mass index (kg/m2) | 23.60 ± 3.50 | 23.89 ± 3.31 | 23.62 ± 3.17 | 23.67 ± 3.14 | 0.18 |
| History of diabetes | 162 (13.9%) | 145 (15.4%) | 162 (15.0%) | 110 (13.8%) | <0.01 |
| History of hypertension | 504 (43.2%) | 408 (43.2%) | 447 (41.4%) | 344 (43.1%) | 0.80 |
| History of stroke | 46 (3.9%) | 46 (4.9%) | 48 (4.4%) | 33 (4.1%) | 0.75 |
| History of heart attack | 123 (10.5%) | 84 (8.9%) | 91 (8.4%) | 94 (11.8%) | 0.06 |
| History of angina | 110 (9.4%) | 83 (8.8%) | 86 (8.0%) | 73 (9.1%) | 0.66 |
| History of congestive heart failure | 42 (3.6%) | 40 (4.2%) | 44 (4.1%) | 25 (3.1%) | 0.61 |
| History of cancer | 47 (4.0%) | 46 (4.9%) | 45 (4.2%) | 38 (4.8%) | 0.74 |
Chi-square (categorical variables) and one-way ANOVA (continuous variables) for subgroup differences. Abbreviations: PASE (Physical Activity Scale for the Elderly); Q (Quartile); SD: standard deviation.
Table 3.
Prospective association of the Portfolio Diet Score with the risk of mortality outcomes among 3991 participants in Mr. OS and Ms. OS Study.
Table 3.
Prospective association of the Portfolio Diet Score with the risk of mortality outcomes among 3991 participants in Mr. OS and Ms. OS Study.
| All-Cause Mortality | CVD Mortality | Cancer Mortality | |||||
|---|---|---|---|---|---|---|---|
| Person-Years | No. of Deaths | HR (95% CI) | No. of Deaths | HR (95% CI) | No. of Deaths | HR (95% CI) | |
| Overall | |||||||
| Q1 (n = 1168) | 13,895 | 458 (39.2%) | 1.00 | 100 (8.6%) | 1.00 | 162 (13.9%) | 1.00 |
| Q2 (n = 944) | 11,772 | 311 (32.9%) | 0.87 (0.75, 1.01) | 71 (7.5%) | 0.91 (0.67, 1.24) | 107 (11.3%) | 0.84 (0.66, 1.08) |
| Q3 (n = 1080) | 13,424 | 388 (35.9%) | 0.96 (0.84, 1.10) | 84 (7.8%) | 0.98 (0.73, 1.31) | 128 (11.9%) | 0.90 (0.71, 1.14) |
| Q4 (n = 799) | 10,317 | 213 (26.7%) | 0.72 (0.61, 0.86) * | 59 (7.4%) | 0.90 (0.64, 1.26) | 72 (9.0%) | 0.72 (0.54, 0.96) * |
| p-value for trend | <0.01 | 0.64 | 0.05 | ||||
| p-value for non-linearity | 0.16 | 0.30 | 0.64 | ||||
| Male | |||||||
| Q1 (n = 620) | 7119 | 300 (48.4%) | 1.00 | 58 (9.4%) | 1.00 | 103 (16.6%) | 1.00 |
| Q2 (n = 405) | 4892 | 173 (42.7%) | 0.94 (0.78, 1.14) | 38 (9.4%) | 1.10 (0.73, 1.66) | 68 (16.8%) | 1.04 (0.76, 1.42) |
| Q3 (n = 576) | 6936 | 259 (45.0%) | 1.02 (0.86, 1.20) | 56 (9.7%) | 1.14 (0.79, 1.66) | 93 (16.1%) | 1.09 (0.82, 1.46) |
| Q4 (n = 395) | 5113 | 120 (30.4%) | 0.63 (0.51, 0.79) * | 36 (9.1%) | 0.90 (0.58, 1.39) | 35 (8.9%) | 0.59 (0.39, 0.87) * |
| p-value for trend | <0.01 | 0.85 | 0.08 | ||||
| p-value for non-linearity | 0.01 | 0.07 | 0.05 | ||||
| Female | |||||||
| Q1 (n = 548) | 6776 | 158 (28.8%) | 1.00 | 42 (7.7%) | 1.00 | 59 (10.8%) | 1.00 |
| Q2 (n = 539) | 6880 | 138 (25.6%) | 0.81 (0.64, 1.02) | 33 (6.1%) | 0.75 (0.47, 1.18) | 39 (7.2%) | 0.65 (0.43, 0.98) * |
| Q3 (n = 504) | 6488 | 129 (25.6%) | 0.84 (0.66, 1.08) | 28 (5.6%) | 0.78 (0.48, 1.28) | 35 (6.9%) | 0.62 (0.40, 0.95) * |
| Q4 (n = 404) | 5204 | 93 (23.0%) | 0.88 (0.67, 1.15) | 23 (5.7%) | 0.92 (0.54, 1.56) | 37 (9.2%) | 0.87 (0.57, 1.34) |
| p-value for trend | 0.34 | 0.65 | 0.35 | ||||
| p-value for non-linearity | 0.25 | 0.54 | <0.01 |
Abbreviations: CVD, cardiovascular disease; HR, hazard ratio. * p < 0.05; The Cox regression model was adjusted for sex, age, dietary energy, body mass index, physical activity, systolic blood pressure, medical history (diabetes, hypertension, stroke, heart attack, angina, congestive heart failure or cancer), smoking habit, alcohol drinking, education level.
Table 4.
Associations between the highest to lowest adherence to the individual components of the Portfolio Diet and risk of mortality.
Table 4.
Associations between the highest to lowest adherence to the individual components of the Portfolio Diet and risk of mortality.
| All-Cause Mortality | CVD Mortality | Cancer Mortality | |
|---|---|---|---|
| HR (95% CI) | HR (95% CI) | HR (95% CI) | |
| Male | |||
| Plant protein sources | 0.77 (0.62, 0.95) * | 0.50 (0.30, 0.81) * | 0.98 (0.69, 1.39) |
| Viscous fiber sources | 0.78 (0.62, 0.98) * | 1.16 (0.73, 1.85) | 0.71 (0.48, 1.04) |
| Nuts | 0.73 (0.58, 0.91) * | 0.91 (0.57, 1.46) | 0.77 (0.54, 1.12) |
| Plant sterols | 0.87 (0.68, 1.10) | 0.88 (0.53, 1.47) | 0.91 (0.61, 1.34) |
| MUFAs sources | 0.94 (0.78, 1.13) | 1.14 (0.79, 1.65) | 0.84 (0.60, 1.18) |
| Saturated fat/cholesterol sources | 0.93 (0.74, 1.18) | 1.06 (0.64, 1.75) | 0.85 (0.56, 1.29) |
| Female | |||
| Plant protein sources | 0.90 (0.68, 1.19) | 1.03 (0.57, 1.84) | 0.70 (0.43, 1.13) |
| Viscous fiber sources | 0.81 (0.61, 1.07) | 1.17 (0.67, 2.05) | 0.81 (0.50, 1.32) |
| Nuts | 0.97 (0.72, 1.30) | 0.63 (0.33, 1.19) | 0.97 (0.59, 1.61) |
| Plant sterols | 0.99 (0.72, 1.36) | 1.36 (0.72, 2.58) | 0.76 (0.44, 1.29) |
| MUFAs sources | 1.12 (0.91, 1.40) | 0.83 (0.51, 1.34) | 1.18 (0.82, 1.70) |
| Saturated fat/cholesterol sources | 1.18 (0.86, 1.61) | 1.53 (0.76, 3.08) | 1.08 (0.63, 1.84) |
Abbreviations: CVD, cardiovascular disease; HR, hazard ratio. * p < 0.05; The Cox regression model was adjusted for sex, age, dietary energy, body mass index, physical activity, systolic blood pressure, medical history (diabetes, hypertension, stroke, heart attack, angina, congestive heart failure or cancer), smoking habit, alcohol drinking, education level.
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Lo, K.; Glenn, A.J.; Yeung, S.; Kendall, C.W.C.; Sievenpiper, J.L.; Jenkins, D.J.A.; Woo, J. Prospective Association of the Portfolio Diet with All-Cause and Cause-Specific Mortality Risk in the Mr. OS and Ms. OS Study. Nutrients 2021, 13, 4360. https://doi.org/10.3390/nu13124360
AMA Style
Lo K, Glenn AJ, Yeung S, Kendall CWC, Sievenpiper JL, Jenkins DJA, Woo J. Prospective Association of the Portfolio Diet with All-Cause and Cause-Specific Mortality Risk in the Mr. OS and Ms. OS Study. Nutrients. 2021; 13(12):4360. https://doi.org/10.3390/nu13124360
Chicago/Turabian StyleLo, Kenneth, Andrea J. Glenn, Suey Yeung, Cyril W. C. Kendall, John L. Sievenpiper, David J. A. Jenkins, and Jean Woo. 2021. "Prospective Association of the Portfolio Diet with All-Cause and Cause-Specific Mortality Risk in the Mr. OS and Ms. OS Study" Nutrients 13, no. 12: 4360. https://doi.org/10.3390/nu13124360
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