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Review

Research Progress of the Biosynthesis of Natural Bio-Antibacterial Agent Pulcherriminic Acid in Bacillus

by
Siqi Yuan
1,2,†,
Xihao Yong
1,†,
Ting Zhao
1,
Yuan Li
1,* and
Jun Liu
1,3,*
1
Sichuan University of Science & Engineering, Xueyuan Street 180#, Huixing Rd., Zigong 643000, China
2
Luzhou Laojiao Group Co. Ltd., Airentang Square, Jiangyang District, Luzhou 646000, China
3
Wuliangye Group Co. Ltd., No. 150 Minjiang West Road, Yibin 644000, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2020, 25(23), 5611; https://doi.org/10.3390/molecules25235611
Submission received: 31 August 2020 / Revised: 13 November 2020 / Accepted: 15 November 2020 / Published: 28 November 2020
(This article belongs to the Special Issue Advances in Research of Short Peptides)
Browse Figures
Review Reports Versions Notes

Abstract

:
Pulcherriminic acid is a cyclic dipeptide found mainly in Bacillus and yeast. Due to the ability of pulcherriminic acid to chelate Fe3+ to produce reddish brown pulcherrimin, microorganisms capable of synthesizing pulcherriminic acid compete with other microorganisms for environmental iron ions to achieve bacteriostatic effects. Therefore, studying the biosynthetic pathway and their enzymatic catalysis, gene regulation in the process of synthesis of pulcherriminic acid in Bacillus can facilitate the industrial production, and promote the wide application in food, agriculture and medicine industries. After initially discussing, this review summarizes current research on the synthesis of pulcherriminic acid by Bacillus, which includes the crystallization of key enzymes, molecular catalytic mechanisms, regulation of synthetic pathways, and methods to improve efficiency in synthesizing pulcherriminic acid and its precursors. Finally, possible applications of pulcherriminic acid in the fermented food, such as Chinese Baijiu, applying combinatorial biosynthesis will be summarized.

1. Introduction

Pulcherriminic acid is derived from cyclo (L-Leu-L-Leu) (cLL), a cyclic dipeptide synthesized by Bacillus and yeast [1,2,3]. The hydroxamic acid group with pulcherriminic acid structure chelates four Fe3+ to form a reddish brown pulcherrimin, thereby the microbes producing pulcherriminic acid can still accumulate iron ions in a severely iron-deficient environment. Since the concentration of iron in the natural environment is relatively low, microorganisms capable for synthesis of pulcherriminic acid compete with other microbes around them for iron through the high affinity of pulcherriminic acid to ferric iron in the environment, and occupy a competitive advantage, making other microorganisms unable to obtain the necessary inorganic salts for growth, especially the iron, which indirectly proves the potential antibacterial activity of pulcherriminic acid [4,5,6,7]. Therefore, Pulcherriminic acid is a very good and safe bioactive antibacterial material in industry.
Pulcherriminic acid is soluble in water, and its molecular formula and molecular weight are C12H20N204 (Figure 1) and 256.2980, respectively, which has maximum UV absorption peaks at wavelengths of 243 nm, 282 nm, and 410 nm [8]. The molecular formula of pulcherrimin is C12H18N204Fe2/3 (Figure 1), and it is reddish brown. Pulcherrimin is almost insoluble in water and organic solvents such as ethanol, but it can be dissolved in alkaline methanol or NaOH solution [8]. The red precipitates gradually appear as the pH of the solution decreases [9]. Pulcherriminic acid was first discovered in the fermentation process of yeast, and it has been found subsequently in Bacillus subtilis, B. cereus, B. licheniformis, and Michia [9,10,11].
In recent years, the synthetic pathways of pulcherriminic acid have been studied extensively in Bacillus, which eliminated the obstacles to increase its production. In 2006, Tang et al. [12] firstly described the synthetic pathway of pulcherriminic acid in B. subtilis, and Gondry et al. [13] showed that YvmC protein in Bacillus was a member of the cyclodipeptide synthases (CDPSs). Bonnefond et al. [14] analyzed the entire synthetic pathway of pulcherriminic acid in Bacillus and proposed the pathway (Figure 1). Leucine (Leu) is a precursor of pulcherriminic acid (Figure 2I), which is synthesized under the catalysis of a series of enzymes with pyruvate as the substrate. These enzymes included acetohydroxyacid synthase (IlvBH), dihydroxy acid dehydratase and keto acid reductase (IlvC and IlvD), 2-isopropylmalate synthase, 3-isopropylmalate dehydrogenase and dehydratase (LeuABCD, Figure 2II) [15]. Leuyl-tRNA synthetase (LeuS) catalyzes the conversion of Leu into leucine-tRNA (Leu-tRNALeu) [16]. Subsequently, two Leu-tRNALeu molecules underwent dehydration condensation to form cLL under the catalysis of CDPSs YvmC. Under the catalysis of the cytochrome P450 oxidase (CypX) that was expressed by the cytochrome P450 synthesis gene cypX downstream of the CDPSs gene yvmC, the formed cLL was finally oxidized to form pulcherriminic acid (Figure 2I) [14]. Finally, the major facilitator superfamily (MFS) transporter (YvmA) transported pulcherriminic acid out of the cell [17,18], and the pulcherriminic acid excreted from the cell was then combined with Fe3+ to form water insoluble pulcherrimin (Figure 2II) [17].
The mechanism for synthesis of cyclic dipeptides has been studied in depth. In Bacillus, YvmC and CypX are involved mainly in the synthesis of pulcherriminic acid, but reports on the transcriptional regulation of yvmC-cypX operon and the secretion of products are still needed further exploration. Due to the results of bacteriostatic studies, it showed that pulcherriminic acid had obviously bacteriostatic activity and could have great potential for applying in the fermented food, insecticides, fruit and vegetable fungicides, new pesticides, and other antibacterial drugs. Surprisingly, pulcherriminic acid and its intermediates or analogs have been determined in Chinese Baijiu (unpublished data). It is a distilled liquor produced by solid state fermentation of natural microorganisms (such as Bacillus and yeasts), thus, the traceability of the functional factors, such as pulcherriminic acid, will become another hot topic for the study of composition of Chinese Baijiu. Overall, clarification of the catalytic and regulatory mechanisms of the key enzymes for the synthesis of pulcherriminic acid could promote the application of pulcherriminic acid in agriculture, food protection and medicine.
The aim of this review is to summary the synthesis pathway and regulatory mechanism of pulcherriminic acid in Bacillus, to illuminate the current research status of the key enzymes in the synthesis of pulcherriminic acid improving the fermentation production, and to pinpoint several specific directions for future research. We hope that this review will provide some guidance for future research on the molecular and regulatory mechanisms for synthesis of pulcherriminic acid.

2. Cyclic Dileucine Synthase is Used to Synthesize the Precursor cLL of Pulcherriminic Acid

2.1. Cyclic Dipeptides

Cyclic dipeptides (also known as cyclodipeptides, 2,5-diketopiperazines or 2,5-dioxopiperazine, CDPs) are the smallest cyclic peptides formed by the condensation of two amino acid molecules through peptide bonds [20,21,22]. They belong to a class of a derivative family known as diketopiperazines (DKPs). DKPs belong to a large class of compounds in nature. Due to their stable six-membered ring structure, DKPs can be used as molecular fragments for other biosynthetic applications, such as molecular drug design [22,23], for antibacterial [24,25], antifungal [26,27], antiviral [28], antitumor [29,30], immunosuppressive [31], neuroprotection [32], antimalarial [33], anti-prion [34], and antihyperglycemic [35] applications, and for scavenging free radicals [36] and other significant physiological/pharmacological activity. With continuous research on DKPs, the biosynthetic pathways of several DKPs have been elucidated. These biosynthetic pathways are generally divided into non-enzymatic and enzymatic pathways. In the non-enzymatic pathway, the cyclization of the peptide chain is spontaneous without the participation of enzymes, such as cyclo(L-His-L-Pro), which is ubiquitous in the central nervous system of higher mammals [37]. Enzyme-catalyzed pathways can be divided into non-ribosomal peptide synthetases (NRPSs) and cyclic dipeptide synthases (CDPSs) [22,38,39]. Based on the current studies, most DKPs are synthesized by NRPSs, which include an adenylation domain, a peptidyl carrier protein domain, and a condensation domain [40].
In recent years, various cyclic dipeptides produced by microorganisms have been reported [41,42]. For example, cyclo (Phe-Pro), cyclo (Tyr-Phe), and cyclo (Leu-Iyr) molecules were used as signal sensors; cyclo (D-Pro-L-Phe) exhibited antibacterial activity, and cyclo (L-Phe-L-Pro) and cyclo (L-Phe-trans-4-OH-Pro) exhibited antifungal activity. cLL is a kind of cyclic dipeptide. It is a small molecule organic cyclization with high stability, protease resistance, and structural rigidity formed by the condensation of two molecules of leucine [22,37,43,44].

2.2. Cyclic Dipeptide Synthases (CDPSs)

In 2002, Lautru et al. [45] discovered an AlbC protein expressed by the albC gene in the genome of Streptomyces noursei, which catalyzes the production of cyclic dipeptides by a non-NRP pathway. Subsequently, Gondry et al. [13] firstly proposed the CDPS pathway [13,45]. In vitro AlbC enzyme activity studies showed that AlbC used aminoacyl tRNAs (aa-tRNAs) as a substrate to synthesize peptide bonds of DKPs, and in silico analysis showed that a variety of bacterial phyla contained AlbC-like proteins. Gene alignment showed that these AlbC-like proteins contained 13 conserved amino acid residues and were divided into two small regions: HX [LVI] [LVI] G [LVI] S and Y [LVI] XXEXP [46]. With the continuous research on CDPSs, we have a deeper understanding of their classification and structure [47].
CDPSs are usually small molecular proteins that consist of 200–300 amino acid residues. Most CDPSs have low sequence homology and <30% amino acid identity [37]. As of 2017, about 800 CDP-encoding genes had been identified through a search of sequence homology, and their number is steadily increasing [41]. They are distributed in all domains, but mainly concentrated in three bacterial phylum (Actinobacteria, Firmicutes, and Proteobacteria) [41]. Among them, about 400 CDPSs originated from Actinomycetes [48,49]. CDPSs are divided into NYH and XYP sub-families based on their Rossmann folds [50]. All CDPSs that have been discovered so far where catalytic function and/or structure have been elucidated belong to the NYH family [50,51].
In 2018, Bourgeois et al. [51] analyzed the CDPSs of the XYP sub-family and compared the structures of the representative enzymes (AlbC and Rgry) in the two subfamilies (NYH and XYP family). The main difference between the two structures was the first half of the Rossmann fold, (Figure 3I–III) [51]. The spatial positions of key catalytic residues (S37, Y202, E182) were the same in the two subfamilies, which indicated that the catalytic mechanism of the two families was probably the same [51]. There was a deep surface-accessible pocket (P1) in the CDPS structure, which was bounded by catalytic residues, and the conservative catalytic residues in its pockets also exhibited good overlap [38,41]. The structure of CDPSs was similar to the type I aminoacyl-tRNA synthetase catalytic domain (class-I aa-tRNA synthases, aa-tRSs), and it was especially similar to class-Ic TyrRSs and TrpRSs [46,52]. The structural differences between CDPSs and aa-tRSs were: (i) CDPSs did not have tRNA-binding domains in type I aa-tRSs, and they relied on surface amino acid residues to bind tRNA, (ii) TyrRSs and TrpRSs generally form homodimers, and the two active sites were in polymer interface, and (iii) the monomers of CDPSs were active [41,50].
The structure of the amino acid binding part of CDPSs was similar to that of aa-tRSs, so they had similar mechanisms for catalyzing the formation of peptide bonds. In particular, the position of the surface accessible P1 pockets in CDPSs was similar to the aminoacyl-binding pockets in aaRS (class-Ic TyrRSs and TrpRSs) [14,16,46]. A possible catalytic mechanism for CDPSs involved using two molecules of aa-tRNA as a substrate, and a cyclic dipeptide was synthesized by a continuous ping-pong reaction [53]. Taking AlbC as an example, AlbC hijacked Phe-tRNAPhe and Phe-tRNAPhe (or the second Leu-tRNALeu) in turn [13] and used them as a substrate for the ping-pong mechanism (Figure 4). This involved the formation of two consecutive acylase intermediates [53]. The first Phe-tRNAPhe bound to an enzyme so that its aminoacyl moiety was contained in the P1 pocket [16]. This portion was then transferred to a conserved serine residue (Ser37) to form the aminoacyl-AlbC intermediate. The second Phe-tRNAPhe bound to the enzyme so that its aminoacyl moiety was located in a wide pocket P2, close to P1 [53]. Then, phenylalanyl-AlbC is reacted with a second Phe-tRNAPhe to form a dipeptidyl-AlbC intermediate, and the final cyclic dipeptide is obtained by intramolecular cyclization. The formation of the first intermediate was demonstrated by trypsinization and peptide fingerprinting (PMF) on AlbC incubated with Phe-tRNAPhe; the second intermediate was captured by modifying a conserved tyrosine (Tyr202) involved in the cyclization process, and this intermediate was detected by PMF analysis using a Tyr202Phe mutant [53]. The catalytic residues identified in the P1 pocket were: Tyr178, Glu182, Asn40, and His203 [53].
Recent studies have shown that the final step of cyclization of cyclic dipeptide depended on the conserved tyrosine residues of CDPSs. As a proton relay, tyrosine residues catalyzed the cyclization reaction through the proton transfer of their hydroxyl groups [51,54]. In 2018, Schmitt et al. [54] studied the cyclization reaction mediated by the cyclic dipeptide synthase AlbC using the quantum mechanics (QM)/molecular mechanics (MM) method and free energy simulation. Experimental results revealed that Tyr202 existed in a neutral protonated form and was unlikely to participate in the catalytic process as a general base; because tyrosine has the unique structure of the benzene ring, the hydroxyl group of tyrosine was more suitable for the proton relay than the hydroxyl group of other amino acids, and the proton was unlikely to transfer directly from the amino group to Ser37 of AlbC [54]. In addition, that the residues Glu182, Tyr178, Asn40, and His203 maintained the correct dipeptide conformation required for the cyclization reaction [54]. More importantly, the functioning of this mechanism depended on the conserved amino groups in the entire CDPS family, and it may be used commonly in CDPSs [54].
The recognition of substrates by CDPSs depended on the type of amino acid and the molecular structure of the corresponding aa-tRNA, which resulted in low substrate specificity of CDPSs [13]. Therefore, several cyclic dipeptide by-products synthesized during the formation of cyclic dipeptides that were catalyzed by CDPSs significantly reduced the synthesis efficiency of the target cyclic amino acids and related metabolites [50]. At present, the structure and catalytic mechanism of some CDPSs have been determined through the analysis of crystal structure, but the kinetic parameters of CDPSs are still unstudied. Through kinetic analysis and structural analysis, it will lay a solid theoretical foundation for the improvement of substrate specificity of CDPSs.

2.3. Cyclic Dileucine Synthase (YvmC)

The AlbC-like protein YvmC, which is synthesized by B. subtilis and B. licheniformis, can use aa-tRNA as a substrate to synthesize cLL-based DKPs [13]. In 2011, Luc Bonnefond et al. [14] obtained the crystal structure of YvmC from B. licheniformis (B. licheniformis CDPSs, YvmC-Blic). X-ray diffraction experiments showed that the YvmC protein of B. licheniformis also had an aaRSs-like catalytic domain (especially class-Ic TyrRSs and TrpRSs) [46,55], which consisted of α / β folds with six stranded spiral units. However, unlike aaRS, the ATP binding motif present in aaRS did not exist in YvmC-Blic, and YvmC-Blic active unit was a monomer instead of a dimer. In addition, YvmC-Blic lacked the last helix and the helical inserts before and after the fourth strand (Figure 5I–II) [14]. Mutation analysis revealed a set of CDPS-specific residues that recognized aminoacyl moieties and catalyzed their transient attachment to conserved serine residues [14,51]. Mass spectrometry analysis, X-ray crystallographic studies, site-directed mutagenesis, and affinity labeling studies have shown that the reaction intermediates were aminoacylase intermediates rather than dipeptidyl tRNA intermediates [14,46,53].
As a buffer substance in the crystallization reagent, 2-cyclohexylaminoethanesulfonic acid (CHES) and 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO) are combined in YvmC-Blic pockets in different ways (Figure 5III,IV) [14]. By analyzing how the two buffer molecules bound to the pocket, Luc Bonnefond et al. concluded that the two buffer molecules mimicked the aminoacyl moiety of the substrate and was bound to the enzyme pocket [14]: every two CHES molecules were bound to one YvmC-Blic monomer. Among them, the interaction between the first CHES molecule and the YvmC-Blic monomer was as follows: (i) the cyclohexane ring of the CHES molecule interacted with the main chains of Gly35 and Leu65 of YvmC-Blic to form a van der Waals force, (ii) the main chain nitrogen atom of the CHES molecule interacted with the side chains of Tyr180 and Glu184 of YvmC-Blic to participate in the formation of hydrogen bonds, (iii) the sulfate oxygen atom in the CHES molecule interacts with the amide group of Arg206 and Asn40 of YvmC-Blic and the hydroxyl group of Tyr204 to form a hydrogen bond.
For the second CHES molecule: (i) the cyclohexane ring of the second CHES molecule interacted hydrophobically with the side chains of Arg206 and Pro207 of YvmC-Blic and with Met131, Glul34, and Ala135 from the symmetric molecule, and (ii) the oxygen atom of the sulfate group of the second CHES molecule formed a hydrogen bond with the main chains of Lys209 and Leu210 of YvmC-Blic [14]. In the structure where CAPSO was combined with YvmC-Blic: (i) the CAPSO cyclohexane ring had a hydrophobic interaction with the main chain of Gly35 and the side chains of Leu65 and Phe188, (ii) the nitrogen and oxygen atoms on the CAPSO main chain interacted with the carboxyl group of Glu184 to form hydrogen bonds, and (iii) an oxygen atom of the sulfate group of CAPSO formed a hydrogen bond with the amino group of Arg206 [14]. By comparing with the crystal structure of Rv2275, Luc Bonnefond et al. found that functionally conserved Tyr180 and Glu184 in YvmC-Blic were used to recognize the amide group of the Leu moiety in Leu-tRNALeu, and Leu202 of YvmC-Blic was involved in substrate-specific recognition [14]. Although the combination of CHES and CAPSO with YvmC-Blic provided a basis for analyzing the binding sites of YvmC-Blic and the substrate, the composite crystallization experiment of Leu-tRNALeu and YvmC is still the gold standard to determine the substrate-specific binding site of YvmC and its interaction mode with the substrate.
Based on comprehensive experimental results, Luc Bonnefond et al. [14] proposed the mechanism of cLL synthesis catalyzed by YvmC-Blic (Figure 6). In the YvmC-Blic catalytic center, the amino group of the leucine residue from the substrate bonded to the side chains of Glu184 and Tyr180 by hydrogen bonding and, at the same time, the amide group of Asn40 polarized the ester carbonyl of leucine to promote its affinity with Ser37 hydroxyl. Subsequently, the leucine residue of the second Leu-RNALeu interacted with the carboxyl group of Glu184 through its amino group; then, the amino group of the first leucine residue attacked the carbonyl ester of the second Leu-tRNALeu, which generated two leucines bound to the enzyme. The two bound leucine residues adopted the cis conformation. Finally, the amide group of the second leucine residue attacked the ester carbonyl of the first leucine residue and cyclized to cLL spontaneously [14]. However, the analysis of experimental results showed that YvmC-Blic exhibited lower substrate specificity because about 40% of the catalytic products were cyclo(L-Leu-L-Phe) and cyclo(L-Leu-L-Met) rather than the target product cLL [14].
In recent years, the number of research reports on YvmC has been very limited, especially with respect to enzymatic characterization, structural analysis and catalytic mechanism of Bacillus YvmC. YvmC is a key enzyme for the synthesis of cLL, which is a precursor of pulcherriminic acid, so in-depth research on YvmC will help to increase the yield of pulcherriminic acid and to promote its industrial production.

3. Cytochrome P450-oxidizing cLL to form pulcherriminic acid

3.1. Cytochrome P450

P450 was first found in mammalian tissue cells, and it was found subsequently in other eukaryotes and prokaryotes. The type of P450 found in eukaryotes was more than that in prokaryotes. Through long-term research on the prokaryote P450, key information about the structure of P450 and the mechanism by which P450 activated dioxygen and oxidized its substrate were gradually revealed [56,57,58].
Due to the many members in the P450 enzyme family, the classification of P450 is divided by the similarity of its amino acid sequences. If the similarity between two members is >40% and <55%, it is judged that they have lower identity, and so they are divided into different families (such as CYP1, CYP2, etc.). If the homology between two members of some P450 families is >55%, those families involved in sequence alignment are defined as subfamilies in a certain P450 family, distinguished by capital letters (CYP1A, etc.); individual members of a subfamily then receive consecutive numbers (CYP1A1, 1A2, etc.) [59,60]. Each member of this subfamily is then divided into bacteria, archaea, yeast/fungi, etc. based on consecutive numbers [59,60]. There are many members of the P450 superfamily, and its amino acid sequences between different species have low conservation. Interestingly, the P450 superfamily contains almost the same structural domains and very similar catalytic functions [61,62]. It is precisely because of the ability of P450 superfamily to catalyze various reactions, resulting in the production of a large number of various secondary metabolites. Moreover, the pulcherrimic acid is one of the secondary metabolites.

3.2. Structure and Catalytic Mechanism of Cytochrome P450 Oxidase-CypX (CYP134A1)

In 2006, Tang et al. [12] identified for the first time the pulcherriminic acid synthetic gene cluster yvmC-cypX in B. subtilis and B. licheniformis by using transcription analysis and bioinformatics. CypX (CYP134A1) is a member of the cytochrome P450 (P450s) blood protein superfamily. It is controlled by the cypX gene, which is a cytochrome P450 synthetic gene located downstream of the gene yvmC.
Because the crystallization of natural B. subtilis CYP134A1 was unsuccessful, Cryle et al. [3] obtained crystals of a selenomethionine-labeled CYP134A1 A356T mutant and used a single abnormal dispersion measurement to determine the B. subtilis structure of CYP134A1 A356T [3]. The structure of CYP134A1 A356T adopted the standard P450 fold, and the main R-helix structure surrounded the non-covalently attached ferro porphyrin IX (heme) part [3] (Figure 7I. The hydrogen bond on the side chain hydroxyl of the mutated Thr356 residue interacted with the main chain carbonyl oxygen of Cys353 and the side chain nitrogen of Asn352. This may be the reason why the A356T mutant formed crystals [3].
CypX structural analysis showed that the sites that usually play an important role in oxygen activation were mainly hydrophobic residues, and the usually conserved alcohol residue was replaced by an unusual proline residue [3]. In addition, an important structural element of CYP134A1 A356T is the N-terminal and central region of the I-helix (residues 229–237). Compared with other P450 structures that are close to CYP134A1 A356T, the above area traverses and occludes more heme surfaces (Figure 7). The other side corresponding to the I-helix is formed by a portion of the β-1 sheet and residues immediately adjacent to the sheet (residues 278–285 (Figure 7). The C-terminus of the F helix forms most of the ceiling above the active site (residues 158-166 (Figure 7). The Tyr391 and Thr392 residues present in the long C-terminal loop of CYP134A1 A356T help to form the upper limit of the active site.
In different structures, Tyr391 existed in different conformations and exhibited side chain orientation, which either pointed to the active site without substrate of CYP134A1, or pointed to the middle position of the substrate’s free orientation (i.e., binding to the benzimidazole ligand) [3]. Due to the hydrogen bond between the phenol group and the glycerol oxygen atom in the crystallization reagent, the position of the tyrosine side chain depended on the existence of glycerol molecules in the active site (Figure 7) [3]. The part of the active site of CYP134A1 A356T that was most exposed to the solvent corresponded to the B-B2 loop region, which included two configurations, “open” and “closed”. In the “open” configuration, the B-B2 loop was substantially parallel to the G helix and did not affect the active site; in the “closed” conformation, the B2 helix was perpendicular to the heme [3] (Figure 7VI). Residues Leu64 and Arg67 projected into the active site cavity (residues 61–73/75 were visible in “open” and “closed” forms, respectively) [3]. A “closed” form was also observed in the structure bound to phenyl imidazole, and the B-B2 ring may be rearranged after substrate binding [3].
The cLL conversion of CYP134A1 A356T in B. subtilis was mediated by electron transfer, and its oxidation product appeared to correspond to the mono-oxidation product of cLL rather than to the expected final product of pulcherriminic acid [3]. The oxidation results using cLL analogs showed that there were different oxidation pathways for CYP134A1 diketopiperazine oxidation. Therefore, the exact mechanism of CYP134A1 in vivo oxidation needs to be studied further. Future studies will explore the wild-type crystal structure and oxidation products of CYP134A1 and combine the enzymatic characterization of CYP134A1 to further clarify the mechanism of the oxidation process [3].
Combining the identification of the biochemical characteristics of CYP134A1, Cryle et al. [3] speculated that the oxidation process of cLL was divided into three steps: first, the nitrogen atoms of the two peptide bonds of cLL was oxidized to form a nitrogen-oxygen structure; immediately afterwards, the above-mentioned nitrogen-oxygen structure removed two molecules of water; then, the six-membered ring was aromatized by intramolecular electron transfer to form pulcherriminic acid (Figure 8) [3].

4. Promotion of Pulcherriminic Acid Production by Metabolic Engineering

Recent research has revealed that the synthesis of pulcherriminic acid was controlled by multiple synthetic gene clusters (Figure 9) [19,63,64]. In 2016, Randazzo et al. [17] demonstrated for the first time that a MarR-like transcriptional regulatory factor encoded by the yvmC-cypX adjacent gene yvmB bound to the yvmC promoter region and inhibit transcription of the yvmC-cypX cluster, which had a direct negative regulatory effect on the synthesis of pulcherriminic acid [17]. AbrB was an important multifunctional negative regulator in Bacillus, and its expression can inhibit the growth and metabolism of cells. The expression level of AbrB was growth-phase-dependent, which remained high during the exponential phase, but decreased when the cells entered the stationary phase [65,66,67].
By knocking out and over-expressing AbrB, Wang et al. [19] found that the synthesis of pulcherriminic acid in B. licheniformis was strictly regulated by AbrB-YvnA-YvmB. AbrB-deficient strains greatly increased the synthesis efficiency of pulcherriminic acid. In addition, AbrB, which is a negative regulator, inhibit the transcription of yvmC-cypX, and it also directly inhibit the transcription of the yvnA gene, which reduced the inhibitory effect of the YvnA protein on the yvmC-cypX operon. With multiple functions, YvnA was at the core of the regulation mechanism for synthesizing Bacillus pulcherriminic acid. Deletion and overexpression of the yvnA gene reduced the efficiency of synthesis of Bacillus pulcherriminic acid. Due to YvnA’s inhibitory effect on yvmB transcription, the yvmC-cypX operon transcribed only at a moderate YvnA concentration. When YvnA concentration was too low, YvmB expression increased and eventually inhibit the synthesis of pulcherriminic acid. Conversely, when the concentration of YvnA was too high, the yvmB and yvmC-cypX operons were suppressed simultaneously. This special dual-regulation system model is called “see-saw regulation” [18,63,64]. In addition, the transcription level of yvnA is affected by the concentration of Fe3+; when the concentration of Fe3+ in the environment was low, the transcription level of yvnA remained low, which resulted in a low concentration of YvnA. When the concentration of Fe3+ in the environment increased, the expression level of yvnA gene increased, and the YvnA concentration also increased [18,64]. Due to the regulatory mechanism of YvnA, there was no excessive production of protic acid to affect the cell’s own growth [68].
YvmA is a transporter protein that belongs to the MFS protein. Deletion of the yvmA gene led to a significant decrease in the production of pulcherriminic acid and an increase in the intracellular accumulation of pulcherriminic acid. The yvmA gene knockout cells still had a small amount of pulcherriminic acid secreted outside the cells, which indicated that the cells also had pulcherriminic acid transporters other than YvmA. The transcription of the yvmA gene itself was also directly negatively regulated by YvmB, and indirectly affected by AbrB and YvnA [18,19,64].
In the process of synthesizing pulcherriminic acid by Bacillus, the synthesis of its intermediate products is crucial to the final product. In the entire Bacillus system, the efficiency of the entire synthetic pathway is regulated by many genes. Regulating the production of target products through transcriptional metabolic engineering is currently the most effective strategy [69,70,71]. Precursor supply plays a key role in metabolite biosynthesis [72]. Due to the inhibition of CodY and TnrA on the ilvBHC-leuABCD operon and the negative feedback regulation of Leu on 2-isopropylmalate synthase LeuA, Leu supplementation in B. licheniformis may be insufficient, which indicated that insufficient substrate supply may be one of the factors that limited the synthesis of pulcherriminic acid [73,74]. In addition, the conversion of Leu to Leu-tRNA may affect the efficiency of peptide and protein synthesis [16].
Vogt et al. [75] obtained a high expression strain of L-Leu by eliminating feedback resistance and deleting the repressor LtbR for L-Leu synthesis. Zhu et al. [76] increased the accumulation of BCAAs in cells by overexpressing BrnQ, which transported branched chain amino acids (BCAA) into cells. Zhu et al. [74] enhanced the biosynthesis of Leu and other substances by deleting the codY gene, and they further explained the negative regulation of amino acid synthesis by CodY. Wang et al. [19] significantly increased the concentration of Leu in cells by over-expressing ilvBHC-leuABCD and deleting bkdAB, and this also increased the production of pulcherriminic acid indirectly. The bkdAB gene encoded the E1 subunit of a branched chain α-keto acid dehydrogenase complex, which converted branched chain amino acids (Leu, Ile, Val) into branched chain α-ketoacyl-CoA initiators (BCCS) and provided raw materials for the branched-chain fatty acid biosynthesis [19,77]. At the same time, the over-expressed aminoacyl-tRNA synthetase was also an effective strategy to promote the synthesis of cLL, which is a precursor of pulcherriminic acid. Xia et al. [78] increased the concentration of glycyl-tRNA by enhancing the expression of glycyl-tRNA synthetase; the overexpression of aminoacyl tRNA synthetase LeuS increased the formation of leucine tRNA. However, high intracellular concentrations of Leu may have triggered feedback inhibition of 2-isopropylmalate synthase LeuA [75]. In 2020, Wang et al. [19] increased the production of pulcherriminic acid to 556.1 mg/L by reconnecting the metabolic pathway of B. licheniformis.

5. Conclusions

As a class of potentially cyclic dipeptide derivatives with antibacterial effect, pulcherriminic acid has great application potential for the food, agriculture and medical industries. At present, the structures of B. licheniformis YvmC and B. subtilis CYP134A1 have been solved and, in addition, the regulatory mechanism for synthesis of pulcherriminic acid in Bacillus has been explored. The determination of the structure and regulatory mechanism of key enzymes for the synthesis of pulcherriminic acid by Bacillus laid a foundation for the industrial production and application of pulcherriminic acid.
However, the key enzymes, synthesis, and regulatory pathways for synthesizing pulcherriminic acid or pulcherrimin from Bacillus are still not completely clear. In view of the above problems, the future research directions for pulcherriminic acid or pulcherrimin was proposed as the following list contents:
  • With the development of synthetic biology and metabolic engineering, biological elements from different sources are introduced into target strains to reconstruct the synthesis pathway of pulcherriminic acid, realize the heterologous expression of YvmC and CypX, and regulate the biosynthesis pathway. When the alien pathway breaks the original metabolic network of the target strain, how to maintain the metabolic balance in the cell and how to regulate the new metabolic network to achieve the best state.
  • In order to achieve the heterogeneous and high yield of pulcherriminic acid, and meet the demands of industrialization, how to screen high activity YvmC and CypX enzyme is critical to the future research. (The catalytic mechanism of Bacillus cyclodipeptide synthetase YvmC and CypX is still unclear, so far).
  • When the YvmA gene is knocked out, the pulcherriminic acid can also be transported out of the cell. Little knowledge is known about the underlying transmembrane mechanism has not been elucidated for pulcherriminic acid. How does pulcherriminic acid get outside of the cell very quickly? It is an urgent problem. What’s the balance mechanism of pulcherriminic acid and the pulcherrimin inside and outside the cell?
  • The directed evolution technique is applied to the enzymes in the synthesis pathway of pulcherriminic acid for the screening of the key synthetases with high catalytic activity.
These are important reasons for the current limitations on the production of pulcherriminic acid. In the future, research on the synthesis pathway of pulcherriminic acid could be more focusing on the clarifying of the catalytic mechanism, structure, and function of each key enzyme, and containing the mechanism of the synthesis, secretion and regulation of pulcherriminic acid. We have reason to believe that the solutions for these problems will promote an increase in the production of pulcherriminic acid and lay the foundation for the industrial production of pulcherriminic acid.

Author Contributions

S.Y., X.Y. and J.L.: conceptualization. X.Y. and T.Z.: software. J.L., X.Y. and Y.L.: validation. S.Y., X.Y., and Y.L.: writing—original draft preparation. J.L., S.Y. and X.Y.: writing—review and editing. J.L. and Y.L.: supervision. J.L.: project administration and funding acquisition. X.Y. and Z.J.: figures modification. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Postdoctoral Research Funding of postdoctoral research station of Sichuan University and Wuliangye Group (Grant No.:205242), the Key Project and Applied Basic Research Project of the Science and Technology Department of Sichuan Province (Grant Nos.:2017NZ0043 and 2020YJ0155), and the APC was funded by the Industry-University-Research Collaboration project of Wuliangye Co., Ltd, (Grant No.:CXY2020ZR02).

Acknowledgments

We would like to thank Thomas A. Gavin, Professor Emeritus, Cornell University, Ziyang Jin, for help with editing this paper.

Conflicts of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Abbreviations

B. cereus: Bacillus cereus; B. subtilis, Bacillus subtilis; B. licheniformis, Bacillus licheniformis; cLL, cyclo(L-Leu-L-Leu); CDPSs, cyclodipeptide synthases; Leu, Leucine; LeuS, Leuyl-tRNA synthetase; Leu-tRNALeu, leucine-tRNA; IlvBH, acetohydroxy acid synthase; IlvC and Ilv D-dihydroxy acid dehydratase and keto acid reductase; LeuABCD-2-isopropylmalate synthase, 3-isopropylmalate dehydrogenase and dehydratase; YvmC, Cyclic dileucine synthase; CypX, cytochrome P450 oxidase; MFS, major facilitator superfamily; DKPs, Diketopiperazines; NRPSs, non-ribosomal peptide synthetases; aa-tRNAs, aminoacyl t-RNAs; PMF, trypsinization and peptide fingerprinting; QM, quantum mechanics; MM, molecular mechanics; CHES, 2-cyclohexylaminoethanesulfonic acid; CAPSO, 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid; P450s, cytochrome P450; BCCS, branched chain α-ketoacyl-CoA initiators; BCAA, branched chain amino acids.

References

  1. Sipiczki, M. Metschnikowia Strains Isolated from Botrytized Grapes Antagonize Fungal and Bacterial Growth by Iron Depletion. Appl. Environ. Microbiol. 2006, 72, 6716–6724. [Google Scholar] [CrossRef] [Green Version]
  2. Uffen, R.L.; Canale-Parola, E. Synthesis of Pulcherriminic Acid by Bacillus subtilis. J. Bacteriol. 1972, 111, 86–93. [Google Scholar] [CrossRef] [Green Version]
  3. Cryle, M.J.; Bell, S.G.; Schlichting, I. Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: A cyclo-L-leucyl-L-leucyl dipeptide oxidase. Biochemistry 2010, 49, 7282–7296. [Google Scholar] [CrossRef]
  4. Melvydas, V.; Staneviciene, R.; Balynaite, A.; Vaiciuniene, J.; Garjonyte, R. Formation of self-organized periodic patterns around yeasts secreting a precursor of a red pigment. Microbiol. Res. 2016, 193, 87–93. [Google Scholar] [CrossRef]
  5. Miethke, M.; Marahiel, M.A. Siderophore-Based Iron Acquisition and Pathogen Control. Microbiol. Mol. Biol. Rev. 2007, 71, 413–451. [Google Scholar] [CrossRef] [Green Version]
  6. Kántor, A.; Hutková, J.; Petrová, J.; Hleba, L.; Kačániová, M. Antimicrobial activity of pulcherrimin pigment produced by Metschnikowia pulcherrima against various yeast species. J. Microbiol. Biotechnol. Food Sci. 2016, 5, 282–285. [Google Scholar] [CrossRef] [Green Version]
  7. Morata, A.; Loira, I.; Escott, C.; Del Fresno, J.M.; Bañuelos, M.A.; Suárez-Lepe, J.A. Applications of Metschnikowia pulcherrima in Wine Biotechnology. Fermentation 2019, 5, 63. [Google Scholar] [CrossRef] [Green Version]
  8. Kluyver, A.J.; Walt, J.P.V.D.; Triet, A.J.V. Pulcherrimin, the Pigment of Candida pulcherrima. Proc. Natl. Acad. Sci. USA 1953, 39, 583–593. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  9. Macdonald, J.C. Biosynthesis of pulcherriminic acid. Biochem. J. 1965, 96, 533–538. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  10. Kupfer, D.G.; Uffen, R.L.; Canale-Parola, E. The role of iron and molecular oxygen in pulcherrimin synthesis by bacteria. Arch. Microbiol. 1967, 56, 9–21. [Google Scholar] [CrossRef] [PubMed]
  11. Krause, D.J.; Kominek, J.; Opulente, D.A.; Shen, X.X.; Zhou, X.; Langdon, Q.K.; DeVirgilio, J.; Hulfachor, A.B.; Kurtzman, C.P.; Rokas, A.; et al. Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts. Proc. Natl. Acad. Sci. USA 2018, 115, 11030–11035. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Tang, M.R.; Sternberg, D.; Behr, R.K.; Sloma, A.; Berka, R.M. Use of transcriptional profiling & bioinformatics to solve production problems: Eliminating red pigment production in a Bacillus subtilis strain producing hyaluronic acid. Ind. Biotechnol. 2006, 2, 66–74. [Google Scholar]
  13. Gondry, M.; Sauguet, L.; Belin, P.; Thai, R.; Amouroux, R.; Tellier, C.; Tuphile, K.; Jacquet, M.; Braud, S.; Courçon, M.; et al. Cyclodipeptide synthases are a family of tRNA-dependent peptide bond–forming enzymes. Nat. Chem. Biol. 2009, 5, 414–420. [Google Scholar] [CrossRef] [PubMed]
  14. Bonnefond, L.; Arai, T.; Sakaguchi, Y.; Suzuki, T.; Ishitani, R.; Nureki, O. Structural basis for nonribosomal peptide synthesis by an aminoacyl-tRNA synthetase paralog. Proc. Natl. Acad. Sci. USA 2011, 108, 3912–3917. [Google Scholar] [CrossRef] [Green Version]
  15. Liu, Z.; Yu, W.; Nomura, C.T.; Li, J.; Chen, S.; Yang, Y.; Wang, Q. Increased flux through the TCA cycle enhances bacitracin production by Bacillus licheniformis DW2. Appl. Microbiol. Biotechnol. 2018, 102, 6935–6946. [Google Scholar] [CrossRef]
  16. Sauguet, L.; Moutiez, M.; Li, Y.; Belin, P.; Seguin, J.; Le Du, M.-H.; Thai, R.; Masson, C.; Fonvielle, M.; Pernodet, J.-L. Cyclodipeptide synthases, a family of class-I aminoacyl-tRNA synthetase-like enzymes involved in non-ribosomal peptide synthesis. Nucleic Acids Res. 2011, 39, 4475–4489. [Google Scholar] [CrossRef] [Green Version]
  17. Randazzo, P.; Aubert-Frambourg, A.; Guillot, A.; Auger, S. The MarR-like protein PchR (YvmB) regulates expression of genes involved in pulcherriminic acid biosynthesis and in the initiation of sporulation in Bacillus subtilis. BMC Microbiol. 2016, 16, 190. [Google Scholar] [CrossRef] [Green Version]
  18. Wang, D.; Zhan, Y.; Cai, D.; Li, X.; Wang, Q.; Chen, S. Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis. Appl. Environ. Microbiol. 2018, 84, e00262-18. [Google Scholar] [CrossRef] [Green Version]
  19. Wang, S.; Wang, H.; Zhang, D.; Li, X.; Zhu, J.; Zhan, Y.; Cai, D.; Wang, Q.; Ma, X.; Wang, D.; et al. Multistep metabolic engineering of Bacillus licheniformis to improve pulcherriminic acid production. Appl. Environ. Microbiol. 2020, 86, e03041-19. [Google Scholar] [CrossRef]
  20. Yao, T.; Liu, J.; Liu, Z.; Li, T.; Li, H.; Che, Q.; Zhu, T.; Li, D.; Gu, Q.; Li, W. Genome mining of cyclodipeptide synthases unravels unusual tRNA-dependent diketopiperazine-terpene biosynthetic machinery. Nat. Commun. 2018, 9, 4091. [Google Scholar] [CrossRef]
  21. Belin, P.; Moutiez, M.; Lautru, S.; Seguin, J.; Pernodet, J.-L.; Gondry, M. The nonribosomal synthesis of diketopiperazines in tRNA-dependent cyclodipeptide synthase pathways. Nat. Prod. Rep. 2012, 29, 961–979. [Google Scholar] [CrossRef] [PubMed]
  22. Giessen, T.; Marahiel, M. The tRNA-Dependent Biosynthesis of Modified Cyclic Dipeptides. Int. J. Mol. Sci. 2014, 15, 14610–14631. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Kamal, R.; Gusain, Y.S.; Kumar, V.; Sharma, A.K. Disease management through biological control agents: An eco-friendly and cost effective approach for sustainable agriculture-A Review. Agric. Rev. 2015, 36, 37. [Google Scholar] [CrossRef]
  24. Abraham, W.R.; De Carvalho, M.P. Antimicrobial and Biofilm Inhibiting Diketopiperazines. Curr. Med. Chem. 2012, 19, 3564–3577. [Google Scholar]
  25. Kohn, H.; Widger, W. The Molecular Basis for the Mode of Action of Bicyclomycin. Curr. Drug Target Infect. Disord. 2005, 5, 273–295. [Google Scholar] [CrossRef] [PubMed]
  26. Musetti, R.; Polizzotto, R.; Vecchione, A.; Borselli, S.; Zulini, L.; D’Ambrosio, M.; Di Toppi, L.S.; Pertot, I. Antifungal activity of diketopiperazines extracted from Alternaria alternata against Plasmopara viticola: An ultrastructural study. Micron 2007, 38, 643–650. [Google Scholar] [CrossRef] [PubMed]
  27. Strom, K.; Sjogren, J.; Broberg, A.; Schnurer, J. Lactobacillus plantarum MiLAB 393 Produces the Antifungal Cyclic Dipeptides Cyclo(L-Phe-L-Pro) and Cyclo(L-Phe-trans-4-OH-L-Pro) and 3-Phenyllactic Acid. Appl. Environ. Microbiol. 2002, 68, 4322–4327. [Google Scholar] [CrossRef] [Green Version]
  28. Rodriguez, P.L.; Carrasco, L. Gliotoxin: Inhibitor of poliovirus RNA synthesis that blocks the viral RNA polymerase 3Dpol. J. Virol. 1992, 66, 1971–1976. [Google Scholar] [CrossRef] [Green Version]
  29. Kanoh, K.; Kohno, S.; Katada, J.; Hayashi, Y.; Uno, I. Biochemistry, Antitumor Activity of Phenylahistin in Vitro and in Vivo. Biosci. Biotechnol. Biochem. 1999, 63, 1130–1133. [Google Scholar] [CrossRef]
  30. Kanzaki, H.; Yanagisawa, S.; Nitoda, T. Enzymatic synthesis of dehydro cyclo(His-Phe)s, analogs of the potent cell cycle inhibitor, dehydrophenylahistin, and their inhibitory activities toward cell division. Biosci. Biotechnol. Biochem. 2004, 68, 2341–2345. [Google Scholar] [CrossRef]
  31. Waring, P.; Beaver, J. Gliotoxin and related epipolythiodioxopiperazines. Gen. Pharmacol. 1996, 27, 1311–1316. [Google Scholar] [CrossRef]
  32. Cornacchia, C.; Cacciatore, I.; Baldassarre, L.; Mollica, A.; Feliciani, F.; Pinnen, F. 2,5-diketopiperazines as neuroprotective agents. Mini Rev. Med. Chem. 2012, 12, 2–12. [Google Scholar] [CrossRef] [PubMed]
  33. Pérez-Picaso, L.; Olivo, H.F.; Argotte-Ramos, R.; Rodríguez-Gutiérrez, M.; Rios, M.Y. Linear and cyclic dipeptides with antimalarial activity. Bioorg. Med. Chem. Lett. 2012, 22, 7048–7051. [Google Scholar] [CrossRef] [PubMed]
  34. Bolognesi, M.L.; Ai Tran, H.N.; Staderini, M.; Monaco, A.; López-Cobeñas, A.; Bongarzone, S.; Biarnés, X.; López-Alvarado, P.; Cabezas, N.; Caramelli, M.; et al. Discovery of a Class of Diketopiperazines as Antiprion Compounds. ChemMedChem 2010, 5, 1324–1334. [Google Scholar] [CrossRef] [PubMed]
  35. Song, M.K.; Hwang, I.K.; Rosenthal, M.J.; Harris, D.M.; Go, V.L.W. Anti-Hyperglycemic Activity of Zinc Plus Cyclo (His-Pro) in Genetically Diabetic Goto-Kakizaki and Aged Rats. Exp. Biol. Med. 2004, 228, 1338–1345. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Furukawa, T.; Akutagawa, T.; Funatani, H.; Uchida, T.; Hotta, Y.; Niwa, M.; Takaya, Y. Cyclic dipeptides exhibit potency for scavenging radicals. Bioorganic Med. Chem. 2012, 20, 2002–2009. [Google Scholar] [CrossRef]
  37. Prasad, C. Bioactive cyclic dipeptides. Peptides 1995, 16, 151–164. [Google Scholar] [CrossRef]
  38. Moutiez, M.; Belin, P.; Gondry, M. Aminoacyl-tRNA-Utilizing Enzymes in Natural Product Biosynthesis. Chem. Rev. 2017, 117, 5578–5618. [Google Scholar] [CrossRef]
  39. Giessen, T.W.; Marahiel, M.A. Rational and combinatorial tailoring of bioactive cyclic dipeptides. Front. Microbiol. 2015, 6, 785. [Google Scholar] [CrossRef]
  40. Kopp, F.; Marahiel, M.A. Macrocyclization strategies in polyketide and nonribosomal peptide biosynthesis. Nat. Prod. Rep. 2007, 24, 735–749. [Google Scholar] [CrossRef]
  41. Zhang, J.; Yao, T.; Liu, J.; Li, H.; Li, W. Recent Advances in Cyclodipeptide Synthases-Dependent Biosynthetic Pathway. Chin. J. Org. Chem. 2019, 39, 328–338. [Google Scholar] [CrossRef]
  42. Mishra, A.; Choi, J.; Choi, S.; Baek, K. Cyclodipeptides: An Overview of Their Biosynthesis and Biological Activity. Molecules 2017, 22, 1796. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Huang, J.H.; Zhuang, S.W.; Fu, J.S.; Wang, C.Y.; Sun, Y.H.; Wang, Y.F. Analysis of aroma compounds of different yeast strains & its molecular identification by bioinformatics. In Proceedings of the 2010 International Conference on Computer and Communication Technologies in Agriculture Engineering, Chengdu, China, 12 June 2010; pp. 25–30. [Google Scholar]
  44. Borthwick, D.A. 2,5-Diketopiperazines: Synthesis, Reactions, Medicinal Chemistry, and Bioactive Natural Products. Chem. Rev. 2012, 112, 3641–3716. [Google Scholar] [CrossRef] [PubMed]
  45. Lautru, S.; Gondry, M.; Genet, R.; Pernodet, J.L. The Albonoursin Gene Cluster of S. noursei: Biosynthesis of Diketopiperazine Metabolites Independent of Nonribosomal Peptide Synthetases. Chem. Biol. 2002, 9, 1355–1364. [Google Scholar] [CrossRef] [Green Version]
  46. Vetting, M.W.; Hegde, S.S.; Blanchard, J.S. The structure and mechanism of the Mycobacterium tuberculosis cyclodityrosine synthetase. Nat. Chem. Biol. 2010, 6, 797–799. [Google Scholar] [CrossRef] [Green Version]
  47. Skinnider, M.A.; Johnston, C.W.; Merwin, N.J.; Dejong, C.A.; Magarvey, N.A. Global analysis of prokaryotic tRNA-derived cyclodipeptide biosynthesis. BMC Genom. 2018, 19, 45. [Google Scholar] [CrossRef] [Green Version]
  48. Brockmeyer, K.; Li, S.-M. Mutations of Residues in Pocket P1 of a Cyclodipeptide Synthase Strongly Increase Product Formation. J. Nat. Prod. 2017, 80, 2917–2922. [Google Scholar] [CrossRef]
  49. Takahito, M.; Noah, R.; Ana, C.; Dieter, S. Bioinformatic Analysis Reveals Archaeal tRNATyr and tRNATrp Identities in Bacteria. Life 2017, 7, 8. [Google Scholar]
  50. Jacques, I.B.; Moutiez, M.; Witwinowski, J.; Darbon, E.; Martel, C.; Seguin, J.; Favry, E.; Thai, R.; Lecoq, A.; Dubois, S.; et al. Analysis of 51 cyclodipeptide synthases reveals the basis for substrate specificity. Nat. Chem. Biol. 2015, 11, 721–727. [Google Scholar] [CrossRef]
  51. Bourgeois, G.; Seguin, J.; Babin, M.; Belin, P.; Moutiez, M.; Mechulam, Y.; Gondry, M.; Schmitt, E. Structural basis for partition of the cyclodipeptide synthases into two subfamilies. J. Struct. Biol. 2018, 203, 17–26. [Google Scholar] [CrossRef]
  52. Rajendran, V.; Kalita, P.; Shukla, H.; Kumar, A.; Tripathi, T. Aminoacyl-tRNA synthetases: Structure, function, and drug discovery. Int. J. Biol. Macromol. 2018, 111, 400–414. [Google Scholar] [CrossRef] [PubMed]
  53. Moutiez, M.; Schmitt, E.; Seguin, J.; Thai, R.; Favry, E.; Belin, P.; Mechulam, Y.; Gondry, M. Unravelling the mechanism of non-ribosomal peptide synthesis by cyclodipeptide synthases. Nat. Commun. 2014, 5, 5141. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Schmitt, E.; Bourgeois, G.; Gondry, M.; Aleksandrov, A. Cyclization Reaction Catalyzed by Cyclodipeptide Synthases Relies on a Conserved Tyrosine Residue. Sci. Rep. 2018, 8, 7031. [Google Scholar] [CrossRef] [PubMed]
  55. Han, W.; Gao, J.F. Research Progress of Cyclodipeptides Synthases. Chem. Bioeng. 2013, 30, 17–21. [Google Scholar]
  56. Poulos, T.L.; Finzel, B.C.; Howard, A.J. High-resolution crystal structure of cytochrome P450cam. J. Mol. Biol. 1987, 195, 687–700. [Google Scholar] [CrossRef]
  57. Rittle, J.; Green, M.T. Cytochrome P450 Compound I: Capture, Characterization, and C-H Bond Activation Kinetics. Science 2010, 330, 933–937. [Google Scholar] [CrossRef] [Green Version]
  58. Mclean, K.J.; Leys, D.; Munro, A.W. Microbial Cytochromes P450, 4th ed. In The Cytochrome P450; Ortiz de Montellano, P., Ed.; Springer: Cham, Switzerland, 2015; pp. 261–407. ISBN 978-3-319-12108-6. [Google Scholar]
  59. Nelson, D.R. Cytochrome P450 Nomenclature, 2004. Methods Mol. Biol. 2006, 320, 1–10. [Google Scholar]
  60. Nelson, D.R. Progress in tracing the evolutionary paths of cytochrome P450. Biochim. Biophys. Acta 2011, 1814, 14–18. [Google Scholar] [CrossRef]
  61. Sezutsu, H.; Le Goff, G.; Feyereisen, R. Origins of P450 diversity. Philos. Trans. Biol. Sci. 2013, 368, 20120428. [Google Scholar] [CrossRef] [Green Version]
  62. Greule, A.; Stok, J.E.; De Voss, J.J.; Cryle, M.J. Unrivalled diversity: The many roles and reactions of bacterial cytochromes P450 in secondary metabolism. Nat. Prod. Rep. 2018, 35, 757–791. [Google Scholar] [CrossRef] [Green Version]
  63. Wang, D.; Wang, Q.; Qiu, Y.; Nomura, C.T.; Li, J.; Chen, S. Untangling the transcription regulatory network of the bacitracin synthase operon in Bacillus licheniformis DW2. Res. Microbiol. 2017, 168, 515–523. [Google Scholar] [CrossRef] [PubMed]
  64. Wang, D. Research of Bacitracin and Pulcherriminc Acid Biosynthesis Regulation in Bacillus. Ph.D. Thesis, Huazhong Agricultural University, Wuhan, China, 2018. [Google Scholar]
  65. Perego, M.; Spiegelman, G.B.; Hoch, J.A. Structure of the gene for the transition state regulator, abrB: Regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis. Mol. Microbiol. 1988, 2, 689–699. [Google Scholar] [CrossRef] [PubMed]
  66. Zuber, P. Specificity through flexibility. Nat. Struct. Biol. 2000, 7, 1079–1081. [Google Scholar] [CrossRef] [PubMed]
  67. O’Reilly, M.; Devine, K.M. Expression of AbrB, a transition state regulator from Bacillus subtilis, is growth phase dependent in a manner resembling that of Fis, the nucleoid binding protein from Escherichia coli. J. Bacteriol. 1997, 179, 522–529. [Google Scholar] [CrossRef] [Green Version]
  68. Arnaouteli, S.; Matoz-Fernandez, D.A.; Porter, M.; Kalamara, M.; Abbott, J.; MacPhee, C.E.; Davidson, F.A.; Stanley-Wall, N.R. Pulcherrimin formation controls growth arrest of the Bacillus subtilis biofilm. Proc. Natl. Acad. Sci. USA 2019, 116, 13553–13562. [Google Scholar] [CrossRef] [Green Version]
  69. Lanza, A.M.; Alper, H.S. Global Strain Engineering by Mutant Transcription Factors. Methods Mol. Biol. 2011, 765, 253–274. [Google Scholar]
  70. Lin, Z.; Zhang, Y.; Wang, J. Engineering of Transcriptional Regulators Enhances Microbial Stress Tolerance. Biotechnol. Adv. 2013, 31, 986–991. [Google Scholar] [CrossRef]
  71. Alper, H.; Stephanopoulos, G. Global transcription machinery engineering: A new approach for improving cellular phenotype. Metab. Eng. 2007, 9, 258–267. [Google Scholar] [CrossRef]
  72. Lynch, S.; Eckert, C.; Yu, J.; Gill, R.; Maness, P.C. Overcoming substrate limitations for improved production of ethylene in E. coli. Biotechnol. Biofuels 2016, 9, 3. [Google Scholar] [CrossRef] [Green Version]
  73. Cai, D.; Zhu, J.; Zhu, S.; Lu, Y.; Zhang, B.; Lu, K.; Li, J.; Ma, X.; Chen, S. Metabolic Engineering of Main Transcription Factors in Carbon, Nitrogen, and Phosphorus Metabolisms for Enhanced Production of Bacitracin in Bacillus licheniformis. ACS Synth. Biol. 2019, 8, 866–875. [Google Scholar] [CrossRef]
  74. Zhu, C.; Xiao, F.; Qiu, Y.; Wang, Q.; He, Z.; Chen, S. Lichenysin production is improved in codY null Bacillus licheniformis by addition of precursor amino acids. Appl. Microbiol. Biotechnol. 2017, 101, 6375–6383. [Google Scholar] [CrossRef] [PubMed]
  75. Vogt, M.; Haas, S.; Klaffl, S.; Polen, T.; Eggeling, L.; Van Ooyen, J.; Bott, M. Pushing product formation to its limit: Metabolic engineering of Corynebacterium glutamicum for L-leucine overproduction. Metab. Eng. 2014, 22, 40–52. [Google Scholar] [CrossRef] [PubMed]
  76. Zhu, J.; Cai, D.; Xu, H.; Liu, Z.; Zhang, B.; Wu, F.; Li, J.; Chen, S. Enhancement of precursor amino acid supplies for improving bacitracin production by activation of branched chain amino acid transporter BrnQ and deletion of its regulator gene lrp in Bacillus licheniformis. Synth. Syst. Biotechnol. 2018, 3, 236–243. [Google Scholar] [CrossRef] [PubMed]
  77. Bentley, G.J.; Jiang, W.; Guamán, L.P.; Xiao, Y.; Zhang, F. Engineering Escherichia coli to produce branched-chain fatty acids in high percentages. Metab. Eng. 2016, 38, 148–158. [Google Scholar] [CrossRef] [Green Version]
  78. Xia, X.X.; Qian, Z.-G.; Ki, C.S.; Park, Y.H.; Kaplan, D.L.; Lee, S.Y. Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber. Proc. Natl. Acad. Sci. USA 2010, 107, 14059–14063. [Google Scholar] [CrossRef] [Green Version]
Molecules 25 05611 g001 550
Figure 1. Binding process of pulcherriminic acid with iron ion (red–brown precipitate: pulcherrimin).
Figure 1. Binding process of pulcherriminic acid with iron ion (red–brown precipitate: pulcherrimin).
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Figure 2. The gene cluster (I) and synthetic pathways (II) of pulcherriminic acid and pulcherrimin in B. subtilis [19].
Figure 2. The gene cluster (I) and synthetic pathways (II) of pulcherriminic acid and pulcherrimin in B. subtilis [19].
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Figure 3. (I) Structure of XYP Rgry-cyclodipeptide synthases (CDPS). The first half of the Rossmann fold is shown in cyan, and the second half of the Rossmann fold is shown in limon. The protein C-terminal positions of residues 236 to 259 are not shown [51]. (II) Structure of NYH CDPS from Streptomyces. The first half of the Rossmann fold is shown in aquamarine, and the second half of the Rossmann fold is shown in marine [51]. (III) To better show the differences between the two subfamilies, the second part of the Rossmann fold of AlbC is superimposed to Rgry-CDPS [51], Rgry is represented by limon, and AlbC is represented by marine.
Figure 3. (I) Structure of XYP Rgry-cyclodipeptide synthases (CDPS). The first half of the Rossmann fold is shown in cyan, and the second half of the Rossmann fold is shown in limon. The protein C-terminal positions of residues 236 to 259 are not shown [51]. (II) Structure of NYH CDPS from Streptomyces. The first half of the Rossmann fold is shown in aquamarine, and the second half of the Rossmann fold is shown in marine [51]. (III) To better show the differences between the two subfamilies, the second part of the Rossmann fold of AlbC is superimposed to Rgry-CDPS [51], Rgry is represented by limon, and AlbC is represented by marine.
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Figure 4. The overall catalytic mechanism of AlbC to produce cyclic dipeptides [21,38]. AlbC (green) uses two aa-tRNAs produced by aaRSs (light red) and is mainly involved in protein synthesis in the ribosome (red with blue). AlbC proceeds through a sequential mechanism that involves the formation of aminoacyl and dipeptidyl enzymes.
Figure 4. The overall catalytic mechanism of AlbC to produce cyclic dipeptides [21,38]. AlbC (green) uses two aa-tRNAs produced by aaRSs (light red) and is mainly involved in protein synthesis in the ribosome (red with blue). AlbC proceeds through a sequential mechanism that involves the formation of aminoacyl and dipeptidyl enzymes.
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Figure 5. (I) The overall structure of YvmC-Blic. Each type of secondary structure has a different color: the ring is light blue, the helix is sky blue, and the strand is pale green [14]; (II) the overall structure of the yeast TyrRS catalytic domain (PDB ID 2DLC), which is similar to the overall structure (A) of YvmC-Blic. Catalytic site-bound tyrosyl-adenylate analogs were drawn using a spherical model [14]; (III) YvmC-Blic with two 2-cyclohexylaminoethanesulfonic acid (CHES) molecules in pockets. Water molecules appear as red spheres. Hydrogen bonds are indicated by yellow dashed lines [14]; (IV) YvmC-Blic with two CAPSO molecules bound to the pocket [14].
Figure 5. (I) The overall structure of YvmC-Blic. Each type of secondary structure has a different color: the ring is light blue, the helix is sky blue, and the strand is pale green [14]; (II) the overall structure of the yeast TyrRS catalytic domain (PDB ID 2DLC), which is similar to the overall structure (A) of YvmC-Blic. Catalytic site-bound tyrosyl-adenylate analogs were drawn using a spherical model [14]; (III) YvmC-Blic with two 2-cyclohexylaminoethanesulfonic acid (CHES) molecules in pockets. Water molecules appear as red spheres. Hydrogen bonds are indicated by yellow dashed lines [14]; (IV) YvmC-Blic with two CAPSO molecules bound to the pocket [14].
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Figure 6. Mechanism of cyclo (L-Leu-L-Leu) (cLL) synthesis catalyzed by B. licheniformis YvmC. The Leucyl moiety of the first and second combined Leu-tRNALeu is highlighted in red and blue, respectively [14].
Figure 6. Mechanism of cyclo (L-Leu-L-Leu) (cLL) synthesis catalyzed by B. licheniformis YvmC. The Leucyl moiety of the first and second combined Leu-tRNALeu is highlighted in red and blue, respectively [14].
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Figure 7. Structure and analysis of CYP134A1 A356T [3]. (I) The overall structure of CYP134A1 A356T (most of CYP134A1 are shown in gray, some structural motifs are colored; heme is shown in red), and the structural motifs are marked [3]. (II) The closed configuration of the B-B2 loop, which indicated that the active site is highly hydrophobic (B-B2 loop residues shown in pink participate in the formation of the active site pocket, and the two conformations of Tyr391 are shown in orange) [3]. (III) Structure of CYP134A1 A356T bound to phenyl imidazole ligand (1-phenylimidazole is shown as a green rod, 2-phenylimidazole is shown as a limon rod) [3]. (IV,V) Effects of the alternate B-B2 loop conformations on the active site pocket (open B-B2 loop and residues that form the active site shown in aquamarine, closed B-B2 loop residues that form the active site shown in darksalmon,Tyr-391 conformations shown in green for the open B-B2 loop and in bright orange for the closed B-B2 loop); for all, hydrogen-bonding distances indicated by yellow dashed lines, heme shown in red, majority of CYP134A1 shown in gray [3]. (VI) Alternative conformation of B-B2 ring (most CYP134A1 is shown in gray, B-B2 ring is shown in slate as closed form and salmon as open form; heme is shown in red), with selected structure Motif mark [3].
Figure 7. Structure and analysis of CYP134A1 A356T [3]. (I) The overall structure of CYP134A1 A356T (most of CYP134A1 are shown in gray, some structural motifs are colored; heme is shown in red), and the structural motifs are marked [3]. (II) The closed configuration of the B-B2 loop, which indicated that the active site is highly hydrophobic (B-B2 loop residues shown in pink participate in the formation of the active site pocket, and the two conformations of Tyr391 are shown in orange) [3]. (III) Structure of CYP134A1 A356T bound to phenyl imidazole ligand (1-phenylimidazole is shown as a green rod, 2-phenylimidazole is shown as a limon rod) [3]. (IV,V) Effects of the alternate B-B2 loop conformations on the active site pocket (open B-B2 loop and residues that form the active site shown in aquamarine, closed B-B2 loop residues that form the active site shown in darksalmon,Tyr-391 conformations shown in green for the open B-B2 loop and in bright orange for the closed B-B2 loop); for all, hydrogen-bonding distances indicated by yellow dashed lines, heme shown in red, majority of CYP134A1 shown in gray [3]. (VI) Alternative conformation of B-B2 ring (most CYP134A1 is shown in gray, B-B2 ring is shown in slate as closed form and salmon as open form; heme is shown in red), with selected structure Motif mark [3].
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Figure 8. Possible mechanism of cytochrome P450 oxidase CypX that catalyzes cLL to form pulcherriminic acid [3].
Figure 8. Possible mechanism of cytochrome P450 oxidase CypX that catalyzes cLL to form pulcherriminic acid [3].
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Figure 9. The synthesis regulation responsible for the synthesis of pulcherriminic acid in Bacillus. The black line with an arrow indicates the synthesis pathway of pulcherriminic acid, the black line with bars indicates inhibition, the black dashed line with bars indicates negative feedback, the red cross indicates deletion inhibition, and the red font indicates missing genes (except Fe3+). The dotted line with arrow indicates the promotion effect, and the dotted line indicates the effect of iron ions on protein. BCAAs, branched chain amino acids.
Figure 9. The synthesis regulation responsible for the synthesis of pulcherriminic acid in Bacillus. The black line with an arrow indicates the synthesis pathway of pulcherriminic acid, the black line with bars indicates inhibition, the black dashed line with bars indicates negative feedback, the red cross indicates deletion inhibition, and the red font indicates missing genes (except Fe3+). The dotted line with arrow indicates the promotion effect, and the dotted line indicates the effect of iron ions on protein. BCAAs, branched chain amino acids.
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Yuan, S.; Yong, X.; Zhao, T.; Li, Y.; Liu, J. Research Progress of the Biosynthesis of Natural Bio-Antibacterial Agent Pulcherriminic Acid in Bacillus. Molecules 2020, 25, 5611. https://doi.org/10.3390/molecules25235611

AMA Style

Yuan S, Yong X, Zhao T, Li Y, Liu J. Research Progress of the Biosynthesis of Natural Bio-Antibacterial Agent Pulcherriminic Acid in Bacillus. Molecules. 2020; 25(23):5611. https://doi.org/10.3390/molecules25235611

Chicago/Turabian Style

Yuan, Siqi, Xihao Yong, Ting Zhao, Yuan Li, and Jun Liu. 2020. "Research Progress of the Biosynthesis of Natural Bio-Antibacterial Agent Pulcherriminic Acid in Bacillus" Molecules 25, no. 23: 5611. https://doi.org/10.3390/molecules25235611

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