Next Article in Journal
Transcriptomic Analysis Reveals JAK2/MPL-Independent Effects of Calreticulin Mutations in a C. elegans Model
Next Article in Special Issue
Vasoactive Soluble Endoglin: A Novel Biomarker Indicative of Reperfusion after Cerebral Large-Vessel Occlusion
Previous Article in Journal
Probiotics Mechanism of Action on Immune Cells and Beneficial Effects on Human Health
Previous Article in Special Issue
Melatonin Attenuates Ischemic-like Cell Injury by Promoting Autophagosome Maturation via the Sirt1/FoxO1/Rab7 Axis in Hippocampal HT22 Cells and in Organotypic Cultures
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Cells
Volume 12
Issue 1
10.3390/cells12010185
cells-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Azilsartan Modulates HMGB1/NF-κB/p38/ERK1/2/JNK and Apoptosis Pathways during Renal Ischemia Reperfusion Injury

by
Rania Alaaeldin
1,
Sally M. Bakkar
2,
Reham H. Mohyeldin
3,
Fares E. M. Ali
4,
Nehad M. Reda Abdel-Maqsoud
5 and
Moustafa Fathy
6,7,*
1
Department of Biochemistry, Faculty of Pharmacy, Deraya University, Minia 61519, Egypt
2
Department of Biochemistry, Faculty of Medicine, Assiut University, Assiut 71515, Egypt
3
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Deraya University, Minia 61519, Egypt
4
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt
5
Department of Pathology, Faculty of Medicine, Deraya University, Minia 61519, Egypt
6
Department of Biochemistry, Faculty of Pharmacy, Minia University, Minia 61511, Egypt
7
Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan
*
Author to whom correspondence should be addressed.
Cells 2023, 12(1), 185; https://doi.org/10.3390/cells12010185
Submission received: 4 December 2022 / Revised: 29 December 2022 / Accepted: 30 December 2022 / Published: 2 January 2023
(This article belongs to the Special Issue Molecular Mechanisms Underlying Organ Damage in Ischemia and Reperfusion Injury)
Browse Figures
Versions Notes

Abstract

Renal ischemia/reperfusion (IR) injury is characterized by an unexpected impairment of blood flow to the kidney. Azilsartan is an angiotensin receptor blocker that is approved for the management of hypertension. The present study aimed to investigate, on molecular basics, the nephroprotective activity of azilsartan on renal IR injury in rats. Rats were assigned into four groups: (1) Sham group, (2) Azilsartan group, (3) IR group, and (4) IR/Azilsartan-treated group. Histological examination and renal function were evaluated. Levels of KIM-1, HMGB1, caspase 3, GPX, SOD, NF-κB, and p53 proteins were investigated using ELISA. mRNA levels of IL-1β, IL6, IL10, TNF-α, NF-κB, p53, and bax were assessed by qRT-PCR. Expression of p38, JNK, and ERK1/2 proteins was investigated by Western blotting. IR injury resulted in tissue damage, elevation of creatinine, BUN, KIM-1, HMGB1, caspase 3, NF-κB, and p53 levels, decreasing GPX and SOD activities, and up-regulation of NF-κB, IL-1β, IL6, TNF-α, p53, and bax genes. Furthermore, it up-regulated the expression of phosphorylated/total ratio of p38, ERK1/2, and JNK proteins. Interestingly, treatment of the injured rats with azilsartan significantly alleviated IR injury-induced histopathological and biochemical changes. It reduced the creatinine, BUN, KIM-1, HMGB1, caspase-3, NF-κB, and p53 levels, elevated GPX and SOD activities, down-regulated the expression of NF-κB, IL-1β, IL6, TNF-α, p53, and bax genes, and up-regulated IL10 gene expression. Furthermore, it decreased the phosphorylated/total ratio of p38, ERK1/2, and JNK proteins. Azilsartan exhibited nephroprotective activity in IR-injured rats via its antioxidant effect, suppression of inflammation, attenuation of apoptosis, and inhibition of HMGB1/NF-κB/p38/ERK1/2/JNK signaling pathway.

1. Introduction

Renal ischemia/reperfusion (IR) injury is a condition characterized by a sudden blood flow impairment to the kidney. It is a prevalent event after kidney transplantation that can lead to delayed graft function, acute kidney injury (AKI), kidney rejection, and long-term risk of graft damage [1]. During renal IR injury, the ischemic damaged tissue produces an excessive amount of free radicals, which impair the mitochondrial electron transport chain, leading to the depletion of ATP and elevation of the intracellular calcium [2]. Additionally, the reperfusion phase can exacerbate the oxidative stress condition with the reactive oxygen species (ROS) generation, resulting in oxidative damage to DNA and proteins, which further initiates apoptotic signaling pathways and cell death [3]. Renal tubular cell apoptosis is also one of the most important pathophysiological processes during IR injury of the kidney [4]. Alteration of apoptotic, oxidative stress, and angiogenic signaling pathways plays an important part in cell survival, tissue restoration, and death [5,6,7,8]. Several antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GPX), oppose the deleterious effect caused by ROS [9,10], thus targeting this pathway could be a beneficial strategy in protecting against tissue damage during IR injury.
Additionally, the initiation of oxidative stress is usually connected with robust inflammation that contributes to the severity of renal damage [11]. Renal IR injury is a systemic inflammatory disease caused by a cascade of pro-inflammatory mediators released from damaged kidney or blood cells. Cytokines, which are critical mediators of immune responses and inflammatory reactions, are implicated in numerous biological processes [12,13,14,15]. Tumor necrosis factor α (TNF-α) and Interleukin 6 (IL-6) are pro-inflammatory mediators involved in renal dysfunction during IR injury [16]. Moreover, activation of nuclear factor-κB (NF-κB) functions a crucial role in numerous pathological processes, including the pathogenesis of IR injury that causes acute renal failure [17,18].
The high mobility group (HMGB1) is a nucleoprotein that is linked to various biological processes, including DNA replication, autophagy, and apoptosis. Under stress conditions, it is translocated to the cytoplasm from the nucleus to be excreted into the extracellular extent and initiate immune response [19]. HMGB1 is a pro-inflammatory factor that acts as a damage-associated molecular pattern molecule activating the innate immune response by triggering numerous receptors on the plasma membrane [20,21]. Its pro-inflammatory role is implicated in a broad range of kidney disorders. It is highly expressed during IR injury, and its upregulation is correlated with injury severity [22]. Additionally, the renal oxidative stress has been accompanied by p38 mitogen-activated protein kinase (p38 MAPK), activation of c-jun N terminal kinase (JNK), reperfusion-induced necrosis, and extracellular signal-regulated kinases (ERK) activation, which can lead to several events, including mitochondrial death and cell apoptosis [23,24].
Moreover, the renin-angiotensin system (RAS) activation and elevation of angiotensin II levels participate in the progression of IR injury. Angiotensin II causes renal vessel constriction, oxidative stress initiation, and apoptosis induction [3,25]. Angiotensin II receptor blockers were described to exhibit protective effects against IR injury of the kidney [26,27].
Screening for new curative potentials of existing candidates has received a lot of attention [28,29,30,31,32,33]. Azilsartan is Food and Drug Administration (FDA)-approved as an antihypertensive candidate for adults. It is a member of angiotensin receptor blockers (ARBs), but it contains an oxo-oxadiazole ring which is not found in any of the clinically approved ARBs [34]. ARBs family has been investigated for its protective effects during IR injury [35,36]. Previously, azilsartan exerted protective effects against cerebral and myocardial IR injuries [37,38]. Additionally, azilsartan attenuated LPS-induced inflammation and oxidative stress in U937 macrophages and lung injury through the modulation of Nr2/HO-1 pathway [39,40]. Furthermore, it showed promising activity in acute myocardial infarction [41] and exerted a potential protective effect against cisplatin-induced cytotoxicity [42]. The present study aimed to examine the effect of azilsartan on renal IR injury in rats and the possible molecular mechanisms implied this effect by inspecting apoptosis and HMGB1/NF-κB/p38/ERK1/2/JNK signaling pathways.

2. Materials and Methods

2.1. Experimental Model

Animal care and study protocols were preceded according to The Declaration of Helsinki’s guidelines and endorsed by the Research Ethics Committee of Minia University, Egypt (ES08-2021). Animals were obtained from the National Research Center (Giza, Egypt), housed in separate cages, fed standard commercial pellets, supplied with fresh drinking water, and kept in a natural environment (12 h light/dark cycles). Renal IR injury was conducted as described before [17]. Pentobarbital sodium (50 mg/kg) was used intraperitoneally to anesthetize animals. To fully explore bilateral renal veins and arteries, a midline laparotomy was implemented. A non-invasive microvascular clamp was used to occlude the left renal pedicle for 30 min. Then, the clamp was loosened. After 48 h, rats were sacrificed to collect renal tissue and serum. Kidney was divided into three portions: The first portion was put in neutral buffered formaldehyde (10%) to be used for histological studies, the second and third tissue portions were kept at −80 °C directly for further protein and RNA analysis.

2.2. Experimental Design

Male Wistar rats were randomly distributed into 4 groups, each of ten rats. The first group (sham group), in which rats orally received carboxy methyl cellulose (CMC) daily for 9 days. The second group (azilsartan group) orally received 4 mg/kg of azilsartan daily [38] for 9 days. The third group (IR group) was the untreated IR group, where rats received CMC daily for 9 days. Then, they underwent 30 min renal ischemia on day 7, followed by 48 h reperfusion. The fourth group (IR/azilsartan group) is the treated IR group, where rats were pretreated orally with 4 mg/kg azilsartan dissolved in carboxy methyl cellulose (CMC) starting 7 days prior to the renal ischemia till the end of reperfusion (the end of the experiment) [38]. The experiment lasted for 9 days whereas, the renal ischemic procedures were performed on day 7.

2.3. Kidney Function Evaluation

Assay kits for creatinine and blood urea nitrogen (BUN) (Randox, London, UK) were utilized according to the manufacturer’s instructions to spectrophotometrically estimate kidney functions.

2.4. ELISA Techniques

Kidney tissues (500 mg) were homogenized in 500 μL of PBS on ice after cutting into small pieces. The supernatant was collected and analyzed after centrifugation at 5000 rpm for 15 min. Activities of GPX and SOD were determined according to the manufacturer’s instructions of the kits (#MBS744364 and #MBS036924, respectively, MyBioSource, San Diego, CA, USA), respectively. HMGB-1 tissue homogenate content was assessed using ELISA determination kit (#E-EL-R0505, Elabscience, Houston, TX, USA). Tissue homogenate caspase-3 and p53 proteins were investigated according to the manufacturer’s instructions for the kits (#CSB-E08857r and #CSB-E08336r, respectively, Cusabio, Houston, TX, USA). Tissue homogenate NF-κB protein was measured according to the manufacturer’s instructions for the kits (#MBS2505513, MyBioSource, San Diego, CA, USA).
Serum Kidney injury molecule-1 (KIM-1) was determined according to the manufacturer’s instructions using the corresponding kits (#CSB-E08808r, Cusabio, Houston, TX, USA).

2.5. Quantitative Real-Time Polymerase Chain Reaction

From renal tissue samples, total RNA was isolated according to the Qiagen RNA extraction kit (Hilden, Germany) instructions. By real-time qPCR, the expression of IL-1β, IL6, TNF-α, NF-κB, p53, and bax genes was investigated. As an internal control, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was utilized [43]. mRNA was quantified using the Rotor-Gene 6000 Series Software 1.7. Primer sequences were obtained from National Centre for Biotechnology Information (NCBI), as shown in Table 1. Qiagen one-step RT-PCR (Qiagen, Hilden, Germany) was utilized for the experiment. Each reaction contained 1× buffer, total RNA (100 ng), 400 μM each of dNTP, 0.6 μM forward and reverse primers, and enzyme mix (2 μL). The experiment was performed under the following conditions: 35 cycles of denaturation step (25 s) at 95 °C, primers annealing (30 s) at 58 °C, and polymerization steps (20 s) at 72 °C.
A melting curve analysis was performed between 60–95 °C at 1 °C intervals, using the SYBR Green fluorescent dye, with the Rotor-Gene 6000 Series Software 1.7 to distinguish the obtained amplified mixture with the avoidance of contamination and to eliminate the production of non-specific compounds. Each sample was performed in triplicate during the experiment, and the average cycle threshold (Ct) was calculated. The target gene expression was calculated for each group relative to the untreated sham group after normalization to GAPDH expression.

2.6. Western Blotting Analysis

The expression of phosphorylated and total forms of p38, ERK 1/2, and JNK proteins was detected utilizing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
To extract proteins from renal tissue samples, RIPA lysis buffer was utilized. The composition of RIPA lysis buffer was as follows: Sodium deoxycholate (0.5%), NaCl (150 mM), SDS (0.1%), PMSF (1 mM), Tris-Cl (50 mM), pH 7.5, and Nonidet P-40 (1%), enriched with the complete protease inhibitor cocktail (Roche, Mannheim, Germany). To estimate the protein concentration, the Bradford method was used [44]. At first, SDS-PAGE (15% acrylamide) was used to separate protein samples (30 μg). Then, proteins were transmitted to a Hybond™ nylon membrane (GE Healthcare) followed by incubation in Blocking Solution at room temperature for 1 h. At 4 °C, membranes were incubated overnight with antibodies of p-p38 (#9211, Cell Signaling Technology, Danvers, MA, USA), p38 (#9212, Cell Signaling Technology, Danvers, MA, USA), p-ERK1/2 (#ab201015, abcam, Cambridge, UK), ERK1/2 (#F54025, NSJ Bioreagents, San Diego, CA, USA), p-JNK (#NB100-82009, Novusbio, Littleton, CO, USA), and JNK (#sc-7345, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) diluted (1:1000) with PBS. A 30–60 min washing period was utilized for membranes followed by an incubation with the diluted (1:1000 in PBS) HRP-conjugated secondary antibody (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature [45]. An enhanced chemiluminescence kit (GE Healthcare, Little Chalfont, UK) was utilized to detect immunoreactive proteins by a luminescent image analyzer (LAS-4000, Fujifilm Co., Tokyo, Japan), according to the manufacturer’s instructions. β-actin, used as an internal control, was detected by β-actin antibody (New England Biolabs) (1:1000). Electroblotting and electrophoresis were carried out in a Bio-Rad Trans-Blot SD Cell apparatus (Bio-Rad, Hercules, CA, USA) using a discontinuous buffer system. Using The Image Processing and Analysis Java (ImageJ, ImageJ bundled with Java 8, 1.8.0_172, Bethesda, Maryland, USA, 1997) program, the densitometric analysis was achieved. Data were obtained relative to the untreated sham group after normalization to the corresponding β-actin levels.

2.7. Histological Examination

In 10% formaldehyde, kidney tissue sections were fixed, then, dehydrated in ascending levels of ethanol and inserted in paraffin. Hematoxylin-eosin (H&E) was used to stain the sections. Then, the histopathological changes were observed using the optical microscope (Leica DMRBE) with the DP Controller software. At least three investigators examined the samples who were blind to pathological information. Tissue sections were evaluated for structural changes in tubules (injury and necrosis), interstitial structural changes, and glomerular changes. The scoring system was performed semi-quantitatively in terms of tubulointerstitial damage as follows: 4 = 76–100%, 3 = 46–75%, 2 = 26–45%, 1 = 0–25%, and 0 = not at all [46].

2.8. Statistical Analysis

Mean ± standard deviation (SD) was used to express the data. To analyze the differences of multiple comparisons, one or two-way analysis of variance (ANOVA) followed by post hoc Dunnett test were performed utilizing Excel software (Microsoft, Redwood, WA, USA) and GraphPad Prism 9 statistical software (GraphPad, La Jolla, CA, USA). When the probability p values < 0.05, differences were considered significant.

3. Results

3.1. Creatinine and BUN Serum Levels

Creatinine and BUN serum levels were significantly increased to 4.81 ± 0.64 mg/dL and 52.4 ± 4.39 mg/dL in the IR group, respectively, when compared to the sham group. While the IR/azilsartan group showed a significant (p < 0.001) decrease in serum levels of both parameters to 2.39 ± 0.34 mg/dL and 27.19 ± 3.89 mg/dL, when compared to the IR group, as shown in Figure 1A non-significant difference (p > 0.05) was found in serum creatinine and BUN levels between the sham group and the azilsartan-treated group.

3.2. Levels of KIM-1, HMGB-1, Caspase 3, GPX, SOD, NF-κB and p53

As shown in Figure 2, when compared to the sham group, the IR group rats showed a significant (p < 0.001) increase in KIM-1, HMGB-1, caspase 3, NF-κB, and p53 protein levels, while levels of GPX and SOD were substantially (p < 0.001) decreased. Treatment of the injured rats with azilsartan decreased (p < 0.001) levels of KIM-1, HMGB-1, and caspase 3 to 1.17 ± 0.12 ng/mL, 47.20 ± 4.6 pg/mL, and 0.87 ± 0.12 ng/mL, respectively, when compared to the IR group. Additionally, levels of GPX and SOD were significantly (p < 0.001) increased in the IR/azilsartan group to 14.67 ± 1.44 ng/mL and 50.91 ± 3.59 U/mL when compared to the IR group. Furthermore, the NF-κB and p53 protein levels were significantly (p < 0.001) diminished in the IR/azilsartan group to 152.4 ± 16.9 pg/mL and 191.8 ± 21.8 pg/mL, respectively, when compared to the IR group. There was no significant difference (p > 0.05) in levels of all measured parameters observed in the azilsartan-treated group, when compared to the sham group.

3.3. Expression of IL-1β, IL6, IL10, TNF-α, NF-κB, p53, and Bax Genes

To investigate the effect of azilsartan on IR injured rats, mRNA levels of IL-1β, IL6, TNF-α, NF-κB, p53, and bax were examined in renal tissues of the different groups. As shown in Figure 3, a significant (p < 0.001) elevation in IL-1β, IL6, TNF-α, NF-κB, p53, and bax genes expression was observed in the IR group rats, while the expression of IL10 gene was significantly (p < 0.01) decreased, when compared to the sham group.
When compared to the IR group, the IR/azilsartan group showed significant (p < 0.001) decrease in IL-1β, IL6, TNF-α, NF-κB, p53, and bax genes expression after normalization to the internal control GAPDH. mRNA levels of IL10 were significantly (p < 0.05) increased in the IR/azilsartan group, when compared to the IR group. Moreover, mRNA levels of all investigated genes showed no significant difference (p > 0.05) between the sham group and the azilsartan group.

3.4. Expression of p38 MAPK, ERK1/2, and JNK Proteins

To further investigate the effect of azilsartan during IR injury and to explain the above-mentioned obtained effects, the expression of phosphorylated and total p38 MAPK, ERK 1/2, and JNK proteins was examined using Western blotting.
In Figure 4, a significant (p < 0.001) renal elevation of phosphor/total ration of p38 MAPK, ERK1/2, and JNK proteins was observed in IR untreated rats compared to sham rats after normalization to β-actin. Remarkably, treating IR-injured rats with azilsartan led to significant (p < 0.001) down-regulation of phosphor/total ratio of p38 MAPK, ERK1/2, and JNK proteins, when compared to the IR group rats.

3.5. Histopathological Examination

For renal histological changes examination, tissues were evaluated using H&E staining to confirm the effect of azilsartan. As shown in Figure 5, (A) the sham group showed normal glomerular tuft with surrounded tubules and normal glomerular basement membrane (arrow), (B) the azilsartan-treated group showed normal tufts, normal interstitium, and no histopathological alterations, compared to rats of the sham group, as shown in Figure 5G. (C) The IR group showed congested tufts (arrow), mild tubular injury with hemorrhage (asterix) and normal interstitium (arrowhead), (D) the IR group showed retracted tufts (arrow), mild tubular necrosis (arrowhead) and interstitial hemorrhage (asterix), and (E) the IR/azilsartan group showed normal tufts (arrow), focal tubular injury (arrowhead) and normal interstitium (asterix).

4. Discussion

Renal IR injury is the primary reason for AKI, which contributes to elevated morbidity and mortality rate. Although the pathophysiology of renal IR injury is not well understood, in the progression of kidney failure, multiple mechanisms are involved [3]. Angiotensin II is a powerful intrarenal vasoconstrictor that induces oxidative stress [47]. It was reported that the oxidative stress indices increase, and renal IR injury were relatively correlated with the angiotensin II levels [48,49]. Therefore, targeting angiotensin II could be beneficial in attenuating renal IR injury. Therefore, various studies have examined the potential activity of ARBs against IR injury, for example, fimasartan and losartan attenuated inflammatory and apoptotic pathways in IR injury via modulating TNFα, IL-1β, and IL6 [50,51]. In addition, valsartan has shown protective activity in IR injury via NF-kB pathway inhibition [35]. Moreover, telmisartan modulated nitric oxide and TNFα during renal IR injury [52].
Looking for new pharmacological activities for novel agents [53,54,55,56] and repurposing drugs [57,58,59] have become a remarkable approach [14,60,61,62]. Azilsartan is a member of ARBs and has been used in the market as an antihypertensive drug [34]. It has been shown to reduce kidney damage and improve glycemic status in diabetic rats [63]. Moreover, in hypertensive obese rats, it decreased cardiovascular and renal injury [63]. Moreover, it exhibited neuroprotective and cardioprotective activities against cerebral and myocardial ischemia [37,38]. Furthermore, it targeted the vascular endothelial growth factor (VEGF) pathway during renal IR injury [27]. Additionally, via the NF-kB/IL-6/JAK2/STAT3 pathway inhibition in breast cancer cells, we recently demonstrated the anticancer activity of azilsartan [64]. Herein, we investigated the effect of azilsartan on apoptosis, ERK/JNK and NF-κB pathways during renal IR injury in rats.
The type 1 transmembrane protein, KIM-1, is only expressed on the proximal tubules in response to various toxins or pathophysiological states. It was proved to be a specific, sensitive kidney injury marker, and an indicator of the outcome as well [65]. Our findings showed that treatment of the IR-injured rats with azilsartan exerted a notable improvement in their renal function, which was represented by decreasing the levels of creatinine, BUN, and especially KIM-1. The histopathological examinations confirmed our hypothesis that renal IR-injured rats treated with azilsartan showed normal glomerulus without inflammatory infiltrates.
The ROS generation during IR injury results in the deleterious effects of IR injury on the cell, with subsequent inflammatory response initiation and AKI development [66]. Additionally, the generation of ROS activates the cell death signaling pathways such as apoptosis and necrosis [67,68]. In the present study, GPX and SOD activities were found to be decreased during IR injury, but after azilsartan treatment, they were notably elevated, which suggests the antioxidant activity of azilsartan, which also has been previously observed through the repression of angiotensin and the oxidative stress [40,69].
It was reported that HMGB1 could link to and stimulate receptors for advanced glycation end products with further stimulation of the ERK, JNK, p38, and NF-κB pathways [70] which, upon activation, lead to the pro-inflammatory cytokines release, including TNF-α, IL-1β, and IL6, and the inflammatory response initiation [6,71,72]. ERK, JNK, and p38, which are also known as stress kinases, are stimulated in response to stress stimuli such as inflammatory cytokines, ROS-mediated oxidative stress, and renal IR injury, which further mediates fibrosis, inflammation, and apoptosis [73,74]. Several studies documented the activation of ERK, JNK, p38 proteins during renal ischemia, thus, targeting this pathway could be a protective approach during IR injury [75,76,77]. Our findings revealed the high expression of NF-κB and HMGB1 proteins and mRNA levels of NF-κB, IL-6, IL-1β, and TNF-α during IR injury, in addition to the increased phosphorylation of ERK1/2, JNK, and p38 proteins, which suggested the activation of these pathways during IR. While IR-injured rats treated with azilsartan showed a notable decrease in HMGB1 and NF-κB proteins, and mRNA levels of NF-κB, IL-6, IL-1β, and TNF-α, in addition to the suppression in the activation and phosphorylation of ERK1/2, JNK, and p38 proteins.
It has been proposed that the activated p38/ERK/JNK signaling pathway contributes directly to cell cycle arrest and p53-mediated apoptosis in cardiomyocytes after myocardial infarction and in Friend murine erythroleukemia virus-transformed cell line [78,79,80]. In the present study, the p53 (protein and mRNA) expression, mRNA levels of bax, and the activity of caspase-3 were elevated, which suggest the induction of apoptosis and cell death during IR injury. At the same time, azilsartan treatment down-regulated the expression of p53 (protein and mRNA) and mRNA bax levels and suppressed the activity of caspase-3, suggesting the anti-apoptotic and protective potential of azilsartan during IR injury.
IL-10, which limits the immune response to external stimuli or pathogen, is a potent anti-inflammatory cytokine, thus, maintaining normal tissue hemostasis and preventing the damaging effect [81]. In the present study, the IL-10 gene expression was found to be suppressed during IR injury, while after azilsartan treatment, its expression was elevated.
The present study took a closer look at the molecular targets of azilsartan during renal IR injury. However, for more recognition of the molecular mechanisms by which azilsartan might extend its valuable renal protection during renal IR injury, future work is required, and additional inspections using different doses of azilsartan are necessary to increase the awareness with regard to the competency of our findings to be clinically applicable.

5. Conclusions

This study demonstrated that azilsartan, which is an FDA-approved antihypertensive drug, exerted promising nephroprotective activity during IR injury in rats via its antioxidant, anti-inflammatory, and anti-apoptotic effects. Our findings revealed that azilsartan showed antioxidant activity by increasing the activity of GPX and SOD. Furthermore, it attenuated inflammation by increasing the expression of IL-10 gene, targeting HMGB1/NF-κB/p38/ERK/JNK signaling pathways and reducing the mRNA levels of NF-κB, IL-6, IL-1β, and TNF-α. Additionally, it suppressed apoptosis by attenuating the levels of p53 and caspase-3 proteins and decreasing mRNA levels of p53 and bax genes.

Author Contributions

Conceptualization, M.F. and R.A.; software, S.M.B.; methodology, R.A. and F.E.M.A.; formal analysis, R.H.M. and N.M.R.A.-M.; validation, R.A., R.H.M. and N.M.R.A.-M.; investigation, F.E.M.A. and S.M.B.; data curation and writing—original draft preparation, R.A.; resources, S.M.B. and N.M.R.A.-M.; writing—review and editing, S.M.B. and M.F.; visualization and supervision, M.F.; project administration and funding acquisition, R.A. and M.F. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Research Ethics Committee of Minia University, Egypt (ES08-2021).

Informed Consent Statement

Not applicable.

Data Availability Statement

All data are fully available and included in the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Tammaro, A.; Kers, J.; Scantlebery, A.M.L.; Florquin, S. Metabolic Flexibility and Innate Immunity in Renal Ischemia Reperfusion Injury: The Fine Balance Between Adaptive Repair and Tissue Degeneration. Front. Immunol. 2020, 11, 1346. [Google Scholar] [CrossRef] [PubMed]
  2. Johnson, K.J.; Weinberg, J.M. Postischemic renal injury due to oxygen radicals. Curr. Opin. Nephrol. Hypertens. 1993, 2, 625–635. [Google Scholar] [CrossRef] [PubMed]
  3. Malek, M.; Nematbakhsh, M. Renal ischemia/reperfusion injury; from pathophysiology to treatment. J. Ren. Inj. Prev. 2015, 4, 20–27. [Google Scholar] [CrossRef] [PubMed]
  4. Xu, M.; Shi, H.; Liu, D. Chrysin protects against renal ischemia reperfusion induced tubular cell apoptosis and inflammation in mice. Exp. Ther. Med. 2019, 17, 2256–2262. [Google Scholar] [CrossRef] [PubMed]
  5. Fathy, M.; Awale, S.; Nikaido, T. Phosphorylated Akt Protein at Ser473 Enables HeLa Cells to Tolerate Nutrient-Deprived Conditions. Asian Pac. J. Cancer Prev. 2017, 18, 3255–3260. [Google Scholar] [CrossRef]
  6. Fathy, M.; Khalifa, E.M.; Fawzy, M.A. Modulation of inducible nitric oxide synthase pathway by eugenol and telmisartan in carbon tetrachloride-induced liver injury in rats. Life Sci. 2019, 216, 207–214. [Google Scholar] [CrossRef]
  7. Fathy, M.; Nikaido, T. In vivo modulation of iNOS pathway in hepatocellular carcinoma by Nigella sativa. Environ. Health Prev. Med. 2013, 18, 377–385. [Google Scholar] [CrossRef]
  8. Fathy, M.; Nikaido, T. In vivo attenuation of angiogenesis in hepatocellular carcinoma by Nigella sativa. Turk. J. Med. Sci. 2018, 48, 178–186. [Google Scholar] [CrossRef]
  9. Abdel-Latif, R.; Fathy, M.; Anwar, H.A.; Naseem, M.; Dandekar, T.; Othman, E.M. Cisplatin-Induced Reproductive Toxicity and Oxidative Stress: Ameliorative Effect of Kinetin. Antioxidants 2022, 11, 863. [Google Scholar] [CrossRef]
  10. Fathy, M.; Darwish, M.A.; Abdelhamid, A.-S.M.; Alrashedy, G.M.; Othman, O.A.; Naseem, M.; Dandekar, T.; Othman, E.M. Kinetin Ameliorates Cisplatin-Induced Hepatotoxicity and Lymphotoxicity via Attenuating Oxidative Damage, Cell Apoptosis and Inflammation in Rats. Biomedicines 2022, 10, 1620. [Google Scholar] [CrossRef]
  11. Fathy, M.; Abdel-Latif, R.; Abdelgwad, Y.M.; Othman, O.A.; Abdel-Razik, A.-R.H.; Dandekar, T.; Othman, E.M. Nephroprotective potential of eugenol in a rat experimental model of chronic kidney injury; targeting NOX, TGF-β, and Akt signaling. Life Sci. 2022, 308, 120957. [Google Scholar] [CrossRef]
  12. El-Baky, R.M.A.; Hetta, H.F.; Koneru, G.; Ammar, M.; Shafik, E.A.; A Mohareb, D.; El-Masry, M.A.; Ramadan, H.K.; Abu Rahma, M.Z.; Fawzy, M.A.; et al. Impact of interleukin IL-6 rs-1474347 and IL-10 rs-1800896 genetic polymorphisms on the susceptibility of HCV-infected Egyptian patients to hepatocellular carcinoma. Immunol. Res. 2020, 68, 118–125. [Google Scholar] [CrossRef]
  13. Eldafashi, N.; Darlay, R.; Shukla, R.; McCain, M.; Watson, R.; Liu, Y.; McStraw, N.; Fathy, M.; Fawzy, M.; Zaki, M.; et al. A PDCD1 Role in the Genetic Predisposition to NAFLD-HCC? Cancers 2021, 13, 1412. [Google Scholar] [CrossRef]
  14. Fathy, M.; Sun, S.; Zhao, Q.-L.; Abdel-Aziz, M.; Abuo-Rahma, G.E.-D.A.; Awale, S.; Nikaido, T. A New Ciprofloxa-cin-derivative Inhibits Proliferation and Suppresses the Migration Ability of HeLa Cells. Anticancer Res. 2020, 40, 5025–5033. [Google Scholar] [CrossRef]
  15. Naseem, M.; Othman, E.M.; Fathy, M.; Iqbal, J.; Howari, F.M.; AlRemeithi, F.A.; Kodandaraman, G.; Stopper, H.; Bencurova, E.; Vlachakis, D.; et al. Integrated structural and functional analysis of the protective effects of kinetin against oxidative stress in mammalian cellular systems. Sci. Rep. 2020, 10, 13330. [Google Scholar] [CrossRef]
  16. Voss, A.; Bode, G.; Kerkhoff, C. Double-Stranded RNA Induces IL-8 and MCP-1 Gene Expression via TLR3 in HaCaT-Keratinocytes. Inflamm. Allergy-Drug Targets 2012, 11, 397–405. [Google Scholar] [CrossRef]
  17. Zhang, J.; Tang, L.; Li, G.S.; Wang, J. The anti-inflammatory effects of curcumin on renal ischemia-reperfusion injury in rats. Ren. Fail. 2018, 40, 680–686. [Google Scholar] [CrossRef]
  18. Abdellatef, A.A.; Fathy, M.; Mohammed, A.E.-S.I.; Abu Bakr, M.S.; Ahmed, A.H.; Abbass, H.S.; El-Desoky, A.H.; Morita, H.; Nikaido, T.; Hayakawa, Y. Inhibition of cell-intrinsic NF-κB activity and metastatic abilities of breast cancer by aloe-emodin and emodic-acid isolated from Asphodelus microcarpus. J. Nat. Med. 2021, 75, 840–853. [Google Scholar] [CrossRef]
  19. Zhao, Z.; Hu, Z.; Zeng, R.; Yao, Y. HMGB1 in kidney diseases. Life Sci. 2020, 259, 118203. [Google Scholar] [CrossRef]
  20. Chen, Q.; Guan, X.; Zuo, X.; Wang, J.; Yin, W. The role of high mobility group box 1 (HMGB1) in the pathogenesis of kidney diseases. Acta Pharm. Sin. B 2016, 6, 183–188. [Google Scholar] [CrossRef]
  21. Zaki, M.Y.W.; Fathi, A.M.; Samir, S.; Eldafashi, N.; William, K.Y.; Nazmy, M.H.; Fathy, M.; Gill, U.S.; Shetty, S. Innate and Adaptive Immunopathogeneses in Viral Hepatitis; Crucial Determinants of Hepatocellular Carcinoma. Cancers 2022, 14, 1255. [Google Scholar] [CrossRef] [PubMed]
  22. Wang, S.; Cai, S.; Zhang, W.; Liu, X.; Li, Y.; Zhang, C.; Zeng, Y.; Xu, M.; Rong, R.; Yang, T.; et al. High-mobility group box 1 protein antagonizes the immunosuppressive capacity and therapeutic effect of mesenchymal stem cells in acute kidney injury. J. Transl. Med. 2020, 18, 175. [Google Scholar] [CrossRef] [PubMed]
  23. Kunduzova, O.R.; Bianchi, P.; Pizzinat, N.; Escourrou, G.; Seguelas, M.H.; Parini, A.; Cambon, C. Regulation of JNK/ERK activation, cell apoptosis, and tissue regeneration by monoamine oxidases after renal ischemia-reperfusion. FASEB J. 2002, 16, 1129–1131. [Google Scholar] [CrossRef] [PubMed]
  24. Fawzy, M.A.; Maher, S.A.; El-Rehany, M.A.; Welson, N.N.; Albezrah, N.K.A.; Batiha, G.E.-S.; Fathy, M. Vincamine Modulates the Effect of Pantoprazole in Renal Ischemia/Reperfusion Injury by Attenuating MAPK and Apoptosis Signaling Pathways. Molecules 2022, 27, 1383. [Google Scholar] [CrossRef] [PubMed]
  25. López, B.; Salom, M.G.; Arregui, B.; Valero, F.; Fenoy, F.J. Role of Superoxide in Modulating the Renal Effects of Angiotensin II. Hypertension 2003, 42, 1150–1156. [Google Scholar] [CrossRef]
  26. Pazoki-Toroudi, H.R.; Hesami, A.; Vahidi, S.; Sahebjam, F.; Seifi, B.; Djahanguiri, B. The preventive effect of captopril or enalapril on reperfusion injury of the kidney of rats is independent of angiotensin II AT1 receptors. Fundam. Clin. Pharmacol. 2003, 17, 595–598. [Google Scholar] [CrossRef]
  27. Ali, A.N.; Altimimi, M.L.; Al-Ardi, H.M.; Hadi, N.R. Nephroprotective Potential Effect of Azilsartan in Renal Ischemia Reperfusion Injury/role VEGF Pathway. Syst. Rev. Pharm. 2019, 10, 90–99. [Google Scholar]
  28. Alaaeldin, R.; Abuo-Rahma, G.E.-D.A.; Zhao, Q.-L.; Fathy, M. Modulation of Apoptosis and Epithelial-Mesenchymal Transition E-cadherin/TGF-β/Snail/TWIST Pathways by a New Ciprofloxacin Chalcone in Breast Cancer Cells. Anticancer. Res. 2021, 41, 2383–2395. [Google Scholar] [CrossRef]
  29. Nagura, S.; Otaka, S.; Koike, C.; Okabe, M.; Yoshida, T.; Fathy, M.; Fukahara, K.; Yoshimura, N.; Misaki, T.; Nikaido, T. Effect of Exogenous Oct4 Overexpression on Cardiomyocyte Differentiation of Human Amniotic Mesenchymal Cells. Cell. Reprogramming 2013, 15, 471–480. [Google Scholar] [CrossRef]
  30. Oba, J.; Okabe, M.; Yoshida, T.; Soko, C.; Fathy, M.; Amano, K.; Kobashi, D.; Wakasugi, M.; Okudera, H. Hyperdry human amniotic membrane application as a wound dressing for a full-thickness skin excision after a third-degree burn injury. Burn. Trauma 2020, 8, tkaa014. [Google Scholar] [CrossRef]
  31. Okabe, M.; Yoshida, T.; Suzuki, M.; Goto, M.; Omori, M.; Taguchi, M.; Toda, A.; Nakagawa, K.; Hiramoto, F.; Ushijima, T.; et al. Hyperdry Human Amniotic Membrane (HD-AM) is Supporting Aciclovir Included Device of Poly-N-p-Vinyl-Benzyl-D-Lactonamide (PVLA) Sphere for Treatment of HSV-1 Infected Rabbit Keratitis Model. J. Biotechnol. Biomater. 2017, 7, 251. [Google Scholar] [CrossRef]
  32. Otaka, S.; Nagura, S.; Koike, C.; Okabe, M.; Yoshida, T.; Fathy, M.; Yanagi, K.; Misaki, T.; Nikaido, T. Selective Isolation of Nanog-Positive Human Amniotic Mesenchymal Cells and Differentiation into Cardiomyocytes. Cell. Reprogramming 2013, 15, 80–91. [Google Scholar] [CrossRef]
  33. Zhou, K.; Koike, C.; Yoshida, T.; Okabe, M.; Fathy, M.; Kyo, S.; Kiyono, T.; Saito, S.; Nikaido, T. Establishment and Characterization of Immortalized Human Amniotic Epithelial Cells. Cell. Reprogramming 2013, 15, 55–67. [Google Scholar] [CrossRef]
  34. Liu, C.; Chen, K.; Wang, H.; Zhang, Y.; Duan, X.; Xue, Y.; He, H.; Huang, Y.; Chen, Z.; Ren, H.; et al. Gastrin Attenuates Renal Ischemia/Reperfusion Injury by a PI3K/Akt/Bad-Mediated Anti-apoptosis Signaling. Front. Pharmacol. 2020, 11, 540479. [Google Scholar] [CrossRef]
  35. AL-SHIBANI, B.I.M.; KAHALEQ, M.A.A.; ABOSAOODA, M.; MOSA, A.K.; ABDULHUSSEIN, M.A.; HADI, N.R. Potential Nephroprotective Effect of Valsartan in Renal Ischemia Reperfusion Injury Role of NF-KBP65 Pathway in Rat. Int. J. Pharm. Res. 2020, 12, 928–935. [Google Scholar]
  36. Morsy, M.A.; Abdel-Gaber, S.A.; Rifaai, R.A.; Mohammed, M.M.; Nair, A.B.; Abdelzaher, W.Y. Protective mechanisms of telmisartan against hepatic ischemia/reperfusion injury in rats may involve PPARγ-induced TLR4/NF-κB suppression. Biomed. Pharmacother. 2022, 145, 112374. [Google Scholar] [CrossRef]
  37. Garg, S.; Khan, S.I.; Malhotra, R.K.; Sharma, M.K.; Kumar, M.; Kaur, P.; Nag, T.C.; Ray, R.; Bhatia, J.; Arya, D.S. Cardiopro-tective effects of azilsartan compared with that of telmisartan on an in vivo model of myocardial ischemia-reperfusion injury. J. Biochem. Mol. Toxicol. 2021, 35, e22785. [Google Scholar] [CrossRef]
  38. Gupta, V.; Dhull, D.K.; Joshi, J.; Kaur, S.; Kumar, A. Neuroprotective potential of azilsartan against cerebral ischemic injury: Possible involvement of mitochondrial mechanisms. Neurochem. Int. 2019, 132, 104604. [Google Scholar] [CrossRef]
  39. Zhang, C.; Zhao, Y.; Yang, X. Azilsartan attenuates lipopolysaccharide-induced acute lung injury via the Nrf2/HO-1 signaling pathway. Immunol. Res. 2022, 70, 97–105. [Google Scholar] [CrossRef]
  40. Dong, Q.; Li, Y.; Chen, J.; Wang, N. Azilsartan Suppressed LPS-Induced Inflammation in U937 Macrophages through Sup-pressing Oxidative Stress and Inhibiting the TLR2/MyD88 Signal Pathway. ACS Omega 2020, 6, 113–118. [Google Scholar] [CrossRef]
  41. Baumann, P.Q.; Zaman, A.T.; McElroy-Yaggy, K.; Sobel, B.E. The efficacy and tolerability of azilsartan in mice with left ven-tricular pressure overload or acute myocardial infarction. J. Cardiovasc. Pharmacol. 2013, 61, 437–443. [Google Scholar] [CrossRef] [PubMed]
  42. Fawzy, M.A.; Beshay, O.N.; Bekhit, A.A.; Abdel-Hafez, S.M.N.; Batiha, G.E.-S.; Bin Jardan, Y.A.; Fathy, M. Nephroprotective effect of AT-MSCs against cisplatin-induced EMT is improved by azilsartan via attenuating oxidative stress and TGF-β/Smad signaling. Biomed. Pharmacother. 2023, 158, 114097. [Google Scholar] [CrossRef] [PubMed]
  43. Barber, R.D.; Harmer, D.W.; Coleman, R.A.; Clark, B.J. GAPDH as a housekeeping gene: Analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol. Genom. 2005, 21, 389–395. [Google Scholar] [CrossRef] [PubMed]
  44. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248–254. [Google Scholar] [CrossRef] [PubMed]
  45. Greenfield, E.A. Antibodies: A Laboratory Manual; Cold Spring Harbor Laboratory Press: New York, NY, USA, 2013. [Google Scholar]
  46. Ozbilgin, S.; Ozkardesler, S.; Akan, M.; Boztas, N.; Ozbilgin, M.; Ergur, B.U.; Derici, S.; Guneli, M.E.; Meseri, R. Renal ische-mia/reperfusion injury in diabetic rats: The role of local ischemic preconditioning. BioMed Res. Int. 2016, 2016, 8580475. [Google Scholar] [CrossRef] [PubMed]
  47. Jaimes, E.A.; Galceran, J.M.; Raij, L. Angiotensin II induces superoxide anion production by mesangial cells. Kidney Int. 1998, 54, 775–784. [Google Scholar] [CrossRef]
  48. Haugen, E.N.; Croatt, A.J.; Nath, K.A. Angiotensin II induces renal oxidant stress in vivo and heme oxygenase-1 in vivo and in vitro. Kidney Int. 2000, 58, 144–152. [Google Scholar] [CrossRef][Green Version]
  49. Kontogiannis, J.; Burns, K.D. Role of AT1angiotensin II receptors in renal ischemic injury. Am. J. Physiol. Physiol. 1998, 274, F79–F90. [Google Scholar] [CrossRef]
  50. Cho, J.-H.; Choi, S.-Y.; Ryu, H.-M.; Oh, E.-J.; Yook, J.-M.; Ahn, J.-S.; Jung, H.-Y.; Choi, J.-Y.; Park, S.-H.; Kim, C.-D. Fimasartan attenuates renal ischemia-reperfusion injury by modulating inflammation-related apoptosis. Korean J. Physiol. Pharmacol. Off. J. Korean Physiol. Soc. Korean Soc. Pharmacol. 2018, 22, 661. [Google Scholar] [CrossRef]
  51. Safari, T.; Shahraki, M.R.; Miri, S.; Bakhshani, N.M.; Niazi, A.A.; Komeili, G.R.; Bagheri, H. The effect of angiotensin 1-7 and losartan on renal ischemic/reperfusion injury in male rats. Res. Pharm. Sci. 2019, 14, 441–447. [Google Scholar] [CrossRef]
  52. Fouad, A.A.; Qureshi, H.A.; Al-Sultan, A.I.; Yacoubi, M.T.; Al-Melhim, W.N. Nephroprotective Effect of Telmisartan in Rats with Ischemia/Reperfusion Renal Injury. Pharmacology 2010, 85, 158–167. [Google Scholar] [CrossRef]
  53. Alaaeldin, R.; Abdel-Rahman, I.A.M.; Hassan, H.A.; Youssef, N.; Allam, A.E.; Abdelwahab, S.F.; Zhao, Q.-L.; Fathy, M. Carpachromene Ameliorates Insulin Resistance in HepG2 Cells via Modulating IR/IRS1/PI3k/Akt/GSK3/FoxO1 Pathway. Molecules 2021, 26, 7629. [Google Scholar] [CrossRef]
  54. Othman, E.; Fathy, M.; Bekhit, A.; Abdel-Razik, A.-R.; Jamal, A.; Nazzal, Y.; Shams, S.; Dandekar, T.; Naseem, M. Modulatory and Toxicological Perspectives on the Effects of the Small Molecule Kinetin. Molecules 2021, 26, 670. [Google Scholar] [CrossRef]
  55. Alaaeldin, R.; Abdel-Rahman, I.M.; Ali, F.E.M.; Bekhit, A.A.; Elhamadany, E.Y.; Zhao, Q.-L.; Cui, Z.-G.; Fathy, M. Dual Topoisomerase I/II Inhibition-Induced Apoptosis and Necro-Apoptosis in Cancer Cells by a Novel Ciprofloxacin Derivative via RIPK1/RIPK3/MLKL Activation. Molecules 2022, 27, 7993. [Google Scholar] [CrossRef]
  56. Alaaeldin, R.; Hassan, H.A.; Abdel-Rahman, I.M.; Mohyeldin, R.H.; Youssef, N.; Allam, A.E.; Abdelwahab, S.F.; Zhao, Q.-L.; Fathy, M. A New EGFR Inhibitor from Ficus benghalensis Exerted Potential Anti-Inflammatory Activity via Akt/PI3K Pathway Inhibition. Curr. Issues Mol. Biol. 2022, 44, 2967–2981. [Google Scholar] [CrossRef]
  57. Alaaeldin, R.; Mustafa, M.; Abuo-Rahma, G.E.A.; Fathy, M. In vitro inhibition and molecular docking of a new ciprofloxacin-chalcone against SARS-CoV-2 main protease. Fundam. Clin. Pharmacol. 2021, 36, 160–170. [Google Scholar] [CrossRef]
  58. Fathy, M.; Okabe, M.; Othman, E.M.; Eldien, H.M.S.; Yoshida, T. Preconditioning of Adipose-Derived Mesenchymal Stem-Like Cells with Eugenol Potentiates Their Migration and Proliferation In Vitro and Therapeutic Abilities in Rat Hepatic Fibrosis. Molecules 2020, 25, 2020. [Google Scholar] [CrossRef]
  59. Shytaj, I.L.; Fares, M.; Gallucci, L.; Lucic, B.; Tolba, M.M.; Zimmermann, L.; Adler, J.M.; Xing, N.; Bushe, J.; Gruber, A.D.; et al. The FDA-Approved Drug Cobicistat Synergizes with Remdesivir To Inhibit SARS-CoV-2 Replication In Vitro and Decreases Viral Titers and Disease Progression in Syrian Hamsters. Mbio 2022, 13, e0370521. [Google Scholar] [CrossRef]
  60. Alaaeldin, R.; Nazmy, M.H.; Abdel-Aziz, M.; Abuo-Rahma, G.E.-D.A.; Fathy, M. Cell Cycle Arrest and Apoptotic Effect of 7-(4-(N-substituted carbamoylmethyl) piperazin-1-yl) Ciprofloxacin-derivative on HCT 116 and A549 Cancer Cells. Anticancer. Res. 2020, 40, 2739–2749. [Google Scholar] [CrossRef]
  61. Eisa, M.A.; Fathy, M.; Nazmy, M.H. Potential COX2 mediated Therapeutic effect of Ciprofloxacin. Minia J. Med. Res. 2021, 32, 47–57. [Google Scholar] [CrossRef]
  62. Fathy, M.; Eldin, S.M.S.; Naseem, M.; Dandekar, T.; Othman, E.M. Cytokinins: Wide-Spread Signaling Hormones from Plants to Humans with High Medical Potential. Nutrients 2022, 14, 1495. [Google Scholar] [CrossRef] [PubMed]
  63. Khan, M.A.H.; Necka, J.; Haines, J.; Imig, J.D.; Khan, A.H.; Neckar, J. Azilsartan Improves Glycemic Status and Reduces Kidney Damage in Zucker Diabetic Fatty Rats. Am. J. Hypertens. 2014, 27, 1087–1095. [Google Scholar] [CrossRef] [PubMed][Green Version]
  64. Alaaeldin, R.; Ali, F.E.; Bekhit, A.A.; Zhao, Q.-L.; Fathy, M. Inhibition of NF-kB/IL-6/JAK2/STAT3 Pathway and Epitheli-al-Mesenchymal Transition in Breast Cancer Cells by Azilsartan. Molecules 2022, 27, 7825. [Google Scholar] [CrossRef] [PubMed]
  65. Bonventre, J.V. Kidney Injury Molecule-1 (KIM-1): A specific and sensitive biomarker of kidney injury. Scand. J. Clin. Lab. Investig. 2008, 68, 78–83. [Google Scholar] [CrossRef] [PubMed]
  66. Tong, F.; Zhou, X. The Nrf2/HO-1 pathway mediates the antagonist effect of L-arginine on renal ischemia/reperfusion injury in rats. Kidney Blood Press. Res. 2017, 42, 519–529. [Google Scholar] [CrossRef] [PubMed]
  67. Redza-Dutordoir, M.; Averill-Bates, D.A. Activation of apoptosis signalling pathways by reactive oxygen species. Biochim. Biophys. Acta Mol. Cell Res. 2016, 1863, 2977–2992. [Google Scholar] [CrossRef]
  68. Saad El dien, H.M.; Bakhaat, G.A.; Rashwan, E.K.; Alaaeldin, R.; Fathy, M. Bone marrow-derived mesenchymal stem cells modulate apoptosis and angiogenesis in cyclophosphamide-induced spleen injury in rats. Egypt. J. Histol. 2022, 45. [Google Scholar] [CrossRef]
  69. Hou, N.; Li, L.-R.; Shi, Y.-Y.; Yuan, W.-C.; Zhao, G.-J.; Liu, X.-W.; Cai, S.-A.; Huang, Y.; Zhan, H.-X.; Pan, W.-B.; et al. Azilsartan ameliorates ventricular hypertrophy in rats suffering from pressure overload-induced cardiac hypertrophy by activating the Keap1–Nrf2 signalling pathway. J. Pharm. Pharmacol. 2021, 73, 1715–1725. [Google Scholar] [CrossRef]
  70. Qu, D.; Ling, Z.; Tan, X.; Chen, Y.; Huang, Q.; Li, M.; Liu, T.; Hou, C.; Chen, Y. High mobility group protein B1 (HMGB1) interacts with receptor for advanced glycation end products (RAGE) to promote airway smooth muscle cell proliferation through ERK and NF-κB pathways. Int. J. Clin. Exp. Pathol. 2019, 12, 3268–3278. [Google Scholar]
  71. Jeong, S.-R.; Park, H.-Y.; Kim, Y.; Lee, K.-W. Methylglyoxal-derived advanced glycation end products induce matrix metalloproteinases through activation of ERK/JNK/NF-κB pathway in kidney proximal epithelial cells. Food Sci. Biotechnol. 2019, 29, 675–682. [Google Scholar] [CrossRef]
  72. Abdel-Hamid, N.M.; Ramadan, M.F.; Amgad, S.W. Glycoregulatory Enzymes as Early Diagnostic Markers during Premalignant Stage in Hepatocellular Carcinoma. Am. J. Cancer Prev. 2013, 1, 14–19. [Google Scholar] [CrossRef]
  73. Liles, J.T.; Corkey, B.K.; Notte, G.T.; Budas, G.R.; Lansdon, E.B.; Hinojosa-Kirschenbaum, F.; Badal, S.S.; Lee, M.; Schultz, B.E.; Wise, S.; et al. ASK1 contributes to fibrosis and dysfunction in models of kidney disease. J. Clin. Investig. 2018, 128, 4485–4500. [Google Scholar] [CrossRef]
  74. Sabra, R.T.; Abdellatef, A.A.; Abdel-Sattar, E.; Fathy, M.; Meselhy, M.R.; Hayakawa, Y. Russelioside A, a Pregnane Glycoside from Caralluma tuberculate, Inhibits Cell-Intrinsic NF-κB Activity and Metastatic Ability of Breast Cancer Cells. Biol. Pharm. Bull. 2022, 45, 1564–1571. [Google Scholar] [CrossRef]
  75. di Mari, J.F.; Davis, R.; Safirstein, R.L. MAPK activation determines renal epithelial cell survival during oxidative injury. Am. J. Physiol. Physiol. 1999, 277, F195–F203. [Google Scholar] [CrossRef]
  76. Feliers, D.; Kasinath, B.S. Erk in Kidney Diseases. J. Signal Transduct. 2011, 2011, 768512. [Google Scholar] [CrossRef]
  77. Fawzy, M.A.; Maher, S.A.; Bakkar, S.M.; El-Rehany, M.A.; Fathy, M. Pantoprazole Attenuates MAPK (ERK1/2, JNK, p38)–NF-κB and Apoptosis Signaling Pathways after Renal Ischemia/Reperfusion Injury in Rats. Int. J. Mol. Sci. 2021, 22, 10669. [Google Scholar] [CrossRef]
  78. Brown, L.; Benchimol, S. The involvement of MAPK signaling pathways in determining the cellular response to p53 activation: Cell cycle arrest or apoptosis. J. Biol. Chem. 2006, 281, 3832–3840. [Google Scholar] [CrossRef]
  79. Sun, A.; Zou, Y.; Wang, P.; Xu, D.; Gong, H.; Wang, S.; Qin, Y.; Zhang, P.; Chen, Y.; Harada, M.; et al. Mitochondrial Aldehyde Dehydrogenase 2 Plays Protective Roles in Heart Failure After Myocardial Infarction via Suppression of the Cytosolic JNK/p53 Pathway in Mice. J. Am. Hear. Assoc. 2014, 3, e000779. [Google Scholar] [CrossRef]
  80. Liu, J.; Mao, W.; Ding, B.; Liang, C.-S. ERKs/p53 signal transduction pathway is involved in doxorubicin-induced apoptosis in H9c2 cells and cardiomyocytes. Am. J. Physiol. Circ. Physiol. 2008, 295, H1956–H1965. [Google Scholar] [CrossRef]
  81. Iyer, S.S.; Cheng, G. Role of Interleukin 10 Transcriptional Regulation in Inflammation and Autoimmune Disease. Crit. Rev. Immunol. 2012, 32, 23–63. [Google Scholar] [CrossRef]
Cells 12 00185 g001
Figure 1. Creatinine (A) and BUN (B) serum levels. Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by one-way ANOVA test, where * p < 0.001, compared to the sham group, and # p < 0.001, compared to the IR group. BUN; blood urea nitrogen, SD; standard deviation, IR; ischemia reperfusion.
Figure 1. Creatinine (A) and BUN (B) serum levels. Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by one-way ANOVA test, where * p < 0.001, compared to the sham group, and # p < 0.001, compared to the IR group. BUN; blood urea nitrogen, SD; standard deviation, IR; ischemia reperfusion.
Cells 12 00185 g001
Cells 12 00185 g002aCells 12 00185 g002b
Figure 2. Levels of KIM-1 (A), HMGB-1 (B), caspase 3 (C), GPX (D), SOD (E), NF-κB (F) and p53 (G). Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by one-way ANOVA test, where * p < 0.001, compared to the sham group, and # p < 0.001, compared to the IR group. KIM-1; kidney injury molecule-1, GPX; glutathione peroxidase, SOD; superoxide dismutase, NF-κB; nuclear factor-κB, SD; standard deviation, IR; ischemia reperfusion.
Figure 2. Levels of KIM-1 (A), HMGB-1 (B), caspase 3 (C), GPX (D), SOD (E), NF-κB (F) and p53 (G). Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by one-way ANOVA test, where * p < 0.001, compared to the sham group, and # p < 0.001, compared to the IR group. KIM-1; kidney injury molecule-1, GPX; glutathione peroxidase, SOD; superoxide dismutase, NF-κB; nuclear factor-κB, SD; standard deviation, IR; ischemia reperfusion.
Cells 12 00185 g002aCells 12 00185 g002b
Cells 12 00185 g003
Figure 3. The renal expression of IL-1β, IL6, IL10, TNF-α, NF-κB, p53, and bax genes. Quantitative RT-PCR was used to investigate the gene expression of different groups. Expression was represented relative to the untreated sham group and normalized to the corresponding GAPDH gene expression. Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by two-way ANOVA test, where **; p < 0.01, ***; p < 0.001, compared to the sham group, and #, p < 0.05; ###, p < 0.001, compared to the IR group. IL; interleukin, TNF-α; tumor necrosis factor-α, NF-κB; nuclear factor-κB, SD; standard deviation, IR; ischemia reperfusion.
Figure 3. The renal expression of IL-1β, IL6, IL10, TNF-α, NF-κB, p53, and bax genes. Quantitative RT-PCR was used to investigate the gene expression of different groups. Expression was represented relative to the untreated sham group and normalized to the corresponding GAPDH gene expression. Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by two-way ANOVA test, where **; p < 0.01, ***; p < 0.001, compared to the sham group, and #, p < 0.05; ###, p < 0.001, compared to the IR group. IL; interleukin, TNF-α; tumor necrosis factor-α, NF-κB; nuclear factor-κB, SD; standard deviation, IR; ischemia reperfusion.
Cells 12 00185 g003
Cells 12 00185 g004
Figure 4. Effect of azilsartan on the expression of p38 MAPK, ERK1/2, and JNK proteins. (A) Representative Western blots of phosphorylated and total p38 MAPK, ERK1/2, JNK, and β-actin proteins for different groups. (B) Expressions of phosphorylated/total ratio of proteins were densitometrically expressed as fold change, using bands in (A), relative to that of sham control rats, after normalization to the corresponding β-actin. Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by two-way ANOVA test, where ***; p < 0.001, compared to the sham group, and ### p < 0.001, compared to the IR group. p38 MAPK; Mitogen Activated Protein Kinase, ERK1/2; extracellular signal-regulated protein kinase, c-JNK; Jun N terminal kinase, SD; standard deviation, IR; ischemia reperfusion.
Figure 4. Effect of azilsartan on the expression of p38 MAPK, ERK1/2, and JNK proteins. (A) Representative Western blots of phosphorylated and total p38 MAPK, ERK1/2, JNK, and β-actin proteins for different groups. (B) Expressions of phosphorylated/total ratio of proteins were densitometrically expressed as fold change, using bands in (A), relative to that of sham control rats, after normalization to the corresponding β-actin. Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by two-way ANOVA test, where ***; p < 0.001, compared to the sham group, and ### p < 0.001, compared to the IR group. p38 MAPK; Mitogen Activated Protein Kinase, ERK1/2; extracellular signal-regulated protein kinase, c-JNK; Jun N terminal kinase, SD; standard deviation, IR; ischemia reperfusion.
Cells 12 00185 g004
Cells 12 00185 g005
Figure 5. Representative photomicrographs of rat kidney tissues of different groups. Hematoxylin-eosin was used to stain kidney tissues. (A) The sham group (magnification; ×200), (B) the azilsartan-treated group (magnification; ×100), (C,D) the IR group (magnification; ×200 & ×100, respectively), and (E) the IR/azilsartan-treated group (magnification; ×100). (F) Histological score of kidney damage. Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by one-way ANOVA test, where * p < 0.001, compared to the sham group, and # p < 0.01, compared to the IR group. SD; standard deviation, IR; ischemia reperfusion.
Figure 5. Representative photomicrographs of rat kidney tissues of different groups. Hematoxylin-eosin was used to stain kidney tissues. (A) The sham group (magnification; ×200), (B) the azilsartan-treated group (magnification; ×100), (C,D) the IR group (magnification; ×200 & ×100, respectively), and (E) the IR/azilsartan-treated group (magnification; ×100). (F) Histological score of kidney damage. Bars represent mean ± SD. Followed by post hoc Dunnett test, significant difference was analyzed by one-way ANOVA test, where * p < 0.001, compared to the sham group, and # p < 0.01, compared to the IR group. SD; standard deviation, IR; ischemia reperfusion.
Cells 12 00185 g005
Table 1. Sequences of the primers.
Table 1. Sequences of the primers.
PrimerSequence of the Primer
IL-1βForward: 5′-CCTATGTCTTGCCCGTGGAG-3’
Reverse: 5′-CACACACTAGCAGGTCGTCA-3′.
IL6Forward: 5′-CCTACCCCAACTTCCAATGCT-3′
Reverse: 5′-GGTCTTGGTCCTTAGCCACT-3′.
IL10Forward: 5′-CTGGCTCAGCACTGCTATGT-3′
Reverse: 5′-GCAGTTATTGTCACCCCGGA-3′.
TNFαForward: 5′-GGAGGGAGAACAGCAACTCC-3′
Reverse: 5′-GCCAGTGTATGAGAGGGACG-3′.
NF-κBForward: 5′-TCAACATGGCAGACGACGATCC-3′
Reverse: 5′-GAAGGTATGGGCCATCTGTTGAC-3′.
P53Forward: 5′-AGCGACTACAGTTAGGGGGT-3′
Reverse: 5′-ACAGTTATCCAGTCTTCAGGGG-3′.
BaxForward: 5′-CGTCTGCGGGGAGTCAC-3′
Reverse: 5′-AGCCATCCTCTCTGCTCGAT-3′.
GAPDHForward: 5′-AACCTGCCAAGTATGATGACATCA-3′
Reverse: 5′-TTCCACTGATATCCCAGCTGCT-3′.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Alaaeldin, R.; Bakkar, S.M.; Mohyeldin, R.H.; Ali, F.E.M.; Abdel-Maqsoud, N.M.R.; Fathy, M. Azilsartan Modulates HMGB1/NF-κB/p38/ERK1/2/JNK and Apoptosis Pathways during Renal Ischemia Reperfusion Injury. Cells 2023, 12, 185. https://doi.org/10.3390/cells12010185

AMA Style

Alaaeldin R, Bakkar SM, Mohyeldin RH, Ali FEM, Abdel-Maqsoud NMR, Fathy M. Azilsartan Modulates HMGB1/NF-κB/p38/ERK1/2/JNK and Apoptosis Pathways during Renal Ischemia Reperfusion Injury. Cells. 2023; 12(1):185. https://doi.org/10.3390/cells12010185

Chicago/Turabian Style

Alaaeldin, Rania, Sally M. Bakkar, Reham H. Mohyeldin, Fares E. M. Ali, Nehad M. Reda Abdel-Maqsoud, and Moustafa Fathy. 2023. "Azilsartan Modulates HMGB1/NF-κB/p38/ERK1/2/JNK and Apoptosis Pathways during Renal Ischemia Reperfusion Injury" Cells 12, no. 1: 185. https://doi.org/10.3390/cells12010185

APA Style

Alaaeldin, R., Bakkar, S. M., Mohyeldin, R. H., Ali, F. E. M., Abdel-Maqsoud, N. M. R., & Fathy, M. (2023). Azilsartan Modulates HMGB1/NF-κB/p38/ERK1/2/JNK and Apoptosis Pathways during Renal Ischemia Reperfusion Injury. Cells, 12(1), 185. https://doi.org/10.3390/cells12010185

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop