Special Issue "Comprehensive and Off-Line Two-Dimensional Liquid Chromatography (2D LC) Separations"

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (28 February 2022) | Viewed by 319

Special Issue Editor

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, CZ-53210 Pardubice, Czech Republic
Interests: HPLC, RP and HILIC retention mechanisms; method development and optimization; monolithic columns; temperature effects; gradient elution; two-dimensional chromatographic separations

Special Issue Information

Dear Colleagues,

Two-dimensional (2D) column separations combining two or more different separation columns can resolve up to one order of magnitude more sample compounds than single one-dimensional separation and, hence, 2D GC and HPLC of several tens to several hundreds of compounds have become quite common in the last decade. In a multidimensional LC offline setup, the columns are independent of each other, whereas in online arrangements, two or more columns are directly coupled using serial, heartcut, stop-and-go, or comprehensive setups. “Comprehensive” online 2D LCxLC chromatography coupling two different separation mechanisms has become a standard technique, which can provide theoretical peak capacities of up to 1500 in about 30–120 min. Remarkable advances were achieved in the development and optimization of column technology and with coupling various LC modes to provide an increasing number of useful 2D LC applications. In addition to the main application field in the analysis of proteins and other biopolymers, comprehensive 2D LC can separate hundreds of compounds in clinical, pharmaceutical, food, natural product, and environmental samples, in molecular biology, in the characterization of biopolymers and synthetic polymers, and in many other fields. In addition to a major increase in peak capacity, multidimensional LC separations may provide group separations of various samples based on molecular structure similarities and differences, which is useful for sample structural analysis and identification.

Prof. Dr. Pavel Jandera
Guest Editor

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • multidimensional LC
  • comprehensive LCxLC
  • peak capacity
  • offline 2D LC
  • serial 2D column coupling
  • stop-and-go LC
  • heartcutting 2D LC
  • 2D LC separation modes
  • 2D LC biopolymer chromatography

Published Papers

There is no accepted submissions to this special issue at this moment.
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