Fluorescence Imaging and Analysis of Cellular System (Volume II)

Dear Colleagues,

The editors are grateful to the many researchers who contributed to the success of the first volume of this Special Issue (https://www.mdpi.com/journal/jimaging/special_issues/fluorescence_imaging). Now, we are very pleased to announce the second volume: “Fluorescence Imaging and Analysis of Cellular System".

Understanding the complicated cellular and molecular processing inside a living cell is crucial for the precise deciphering of the intricate network of information that drives the biology of a living system. Cellular fluorescence imaging emerged as a tool that enables the integration of biological complexity into drug discovery by unravelling the mystery of the live cell. The most widely applied technique for the cellular imaging of live cells is the use of fluorescent proteins (FPs) to light up cellular structures such as organelles or biomolecules. In order to identify and track biomolecules in the complex environment of the cell, molecular specificity is essential. Encouraged by the noteworthy benefits that FPs provide for cellular imaging, there has also been a focus on developing methods for the labelling of biomolecules with small-molecule probes, enabling greater labelling refinement, and for extending fluorescent tagging to more diverse biomolecules. One such effort includes bio-orthogonal labelling, which is the use of diverse methodologies for labelling cellular constituents in vitro and in vivo with exceptional chemical probes such as fluorophores or cross-linking reagents. Such fluorophore/fluorescent probes must be compatible with the cellular environment, show strong fluorescence and be photostable, while also being nontoxic and permeable across cellular and organellar membranes.

This Special Issue of the Journal of Imaging aims to feature reports of new developments in live cell fluorescence imaging systems/methods to accomplish bio-orthogonal labelling and imaging by using chemical probes such as organic fluorophores, FPs, nanoprobes, and quantum dots.

Dr. Ashutosh Sharma
Guest Editor

Manuscript Submission Information

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• fluorescence imaging
• bio-orthogonal labeling
• fluorescent protein
• chemical probes for imaging
• fluorophores for imaging
• imaging tools for live cells