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Antibodies
Special Issues
Development and Application of Antibody Against Small Molecule Phytoproduct
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Development and Application of Antibody Against Small Molecule Phytoproduct

A special issue of Antibodies (ISSN 2073-4468).

Deadline for manuscript submissions: closed (30 November 2021) | Viewed by 6919

Special Issue Editors

Prof. Dr. Yukihiro Shoyama

E-Mail Website
Guest Editor
Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan
Interests: monoclonal antibodies; bioactive compounds; marihuana study; isolation and structure elucidation; biotechnology.
Prof. Dr. Hiroyuki Tanaka

E-Mail Website
Guest Editor
Faculty of Pharmaceutical Sciences, Sanyo-Onoda City University, Yamaguchi, Japan
Interests: natural products; monoclonal antibodies; Kampo; medicinal plants
Dr. Seiichi Sakamoto

E-Mail Website
Guest Editor
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Interests: allelopathy; antibodies; molecular bioimprinting; phytochemical analysis

Special Issue Information

Dear Colleagues,

A Special Issue related to monoclonal antibodies will be published in Antibodies. The research area includes monoclonal antibodies against natural products with a small molecule such as saponins, alkaloids, phenolics, terpenoids, and so on. Furthermore, work exploring the compact antibody and single-chain Fv is also welcome. This Special Issue will be an opportunity to discuss future developments in natural product research.

Antibodies has launched a Special Issue Development and Application of Monoclonal Antibodies Against Small-Molecule Phytoproducts to spread and innovate natural product research using monoclonal antibodies. Monoclonal antibodies against small-molecule natural products have accumulated since the 1990s, resulting in much interesting evidence related to natural product research using monoclonal antibodies. In this context, this Special Issue encourages the discussion of new research in the field of natural products. Colleagues in the field of natural products are invited to contribute their work discussing and debating natural product research.

Prof. Dr. Yukihiro Shoyama
Prof. Dr. Hiroyuki Tanaka
Dr. Seiichi Sakamoto
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibodies is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Monoclonal antibodies 
  • Small molecule natural product 
  • Development and application 
  • Qualitative and quantitative analysis
  • Analytical kit 
  • Affinity column 
  • Affinity isolation 
  • Compact antibody 
  • Single-chain Fv

Published Papers (2 papers)

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Review

13 pages, 12124 KiB  
Review
Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies
by Takuhiro Uto, Tomoe Ohta, Shunsuke Fujii and Yukihiro Shoyama
Antibodies 2021, 10(4), 48; https://doi.org/10.3390/antib10040048 - 6 Dec 2021
Cited by 2 | Viewed by 2969
Abstract
In this study, we present a review on a useful approach, namely, immunoaffinity column coupled with monoclonal antibodies (MAbs), to separate natural compounds and its application for cell-based studies. The immunoaffinity column aids in separating the specific target compound from the crude extract. [...] Read more.
In this study, we present a review on a useful approach, namely, immunoaffinity column coupled with monoclonal antibodies (MAbs), to separate natural compounds and its application for cell-based studies. The immunoaffinity column aids in separating the specific target compound from the crude extract. The column capacity was stable even after more than 10 purification cycles of use under the same conditions. After applying the crude extract to the column, the column was washed with washing buffer and eluted with elution buffer. The elution fraction contained the target compound bound to MAb, whereas the washing fraction was the crude extract, which contained all compounds except a group of target compounds; therefore, the washing fraction was referred to as a knockout (KO) crude extract. Cell-based studies using the KO extract revealed the actual effects of the natural compounds in the crude extract. One-step separation of natural compounds using the immunoaffinity column coupled with MAbs may help in determining the potential functions of natural compounds in crude extracts. Full article
(This article belongs to the Special Issue Development and Application of Antibody Against Small Molecule Phytoproduct)
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10 pages, 2728 KiB  
Review
Studies on Natural Products Using Monoclonal Antibodies: A Review
by Yukihiro Shoyama
Antibodies 2021, 10(4), 43; https://doi.org/10.3390/antib10040043 - 1 Nov 2021
Viewed by 3338
Abstract
An immunoblotting system (“eastern blotting”) was developed for small-molecule herbal medicines like glycosides, with no conjugation function to the membrane. Briefly, the crude extracts of herb medicines were developed by thin-layer chromatography (TLC). The small-molecule herbal medicines on TLC plates were transferred to [...] Read more.
An immunoblotting system (“eastern blotting”) was developed for small-molecule herbal medicines like glycosides, with no conjugation function to the membrane. Briefly, the crude extracts of herb medicines were developed by thin-layer chromatography (TLC). The small-molecule herbal medicines on TLC plates were transferred to polyvinylidene fluoride (PVDF) or polyethersulfone (PES) membranes by heating. Antigen components were divided into two categories based on their function, i.e., their membrane recognizing (aglycone part) and fixing (sugar moiety) abilities. This procedure allows for the staining of only target glycosides. Double eastern blotting was developed as a further staining system for two herb medicines using a set of MAbs and substrates. Full article
(This article belongs to the Special Issue Development and Application of Antibody Against Small Molecule Phytoproduct)
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