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Article

The Interactions of Magnesium Sulfate and Cromoglycate in a Rat Model of Orofacial Pain; The Role of Magnesium on Mast Cell Degranulation in Neuroinflammation

by
Dragana Srebro
1,
Branko Dožić
2,*,
Sonja Vučković
1,
Katarina Savić Vujović
1,
Branislava Medić Brkić
1,
Ivan Dožić
3 and
Milorad Srebro
1
1
Department of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Belgrade, Dr Subotića-Starijeg 1, 11129 Belgrade, Serbia
2
Department of Pathology, School of Dental Medicine, University of Belgrade, Dr Subotića-Starijeg 1, 11000 Belgrade, Serbia
3
Department of Biochemistry, School of Dental Medicine, University of Belgrade, Dr Subotića-Starijeg 1, 11000 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(7), 6241; https://doi.org/10.3390/ijms24076241
Submission received: 20 January 2023 / Revised: 16 March 2023 / Accepted: 20 March 2023 / Published: 26 March 2023
(This article belongs to the Special Issue The Role of Mg Homeostasis in Disease)
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Abstract

Mast cell degranulation impacts the development of pain and inflammation during tissue injury. We investigated the antinociceptive effect of a combination of cromoglycate and magnesium in the orofacial model of pain and the histological profile of the effect of magnesium in orofacial pain. In male Wistar rats, formalin (1.5%, 100 µL) was injected subcutaneously into the right upper lip of rats after cromoglycate and/or magnesium. Pain was measured as the total time spent on pain-related behavior. Toluidine blue staining was used to visualize mast cells under the light microscope. In the formalin test, in phase 1, magnesium antagonized the antinociceptive effect of cromoglycate, while in phase 2, it potentiated or inhibited its effect. Magnesium significantly reduced mast cell degranulation in the acute phase by about 23% and in the second phase by about 40%. Pearson’s coefficient did not show a significant correlation between mast cell degranulation and pain under treatment with magnesium. The cromoglycate–magnesium sulfate combination may prevent the development of inflammatory orofacial pain. The effect of a combination of cromoglycate–magnesium sulfate depends on the nature of the pain and the individual effects of the drugs. Magnesium reduced orofacial inflammation in the periphery, and this effect did not significantly contribute to its analgesic effect.
Keywords: trigeminal pain; mast cell; neuroinflammation; nociception; magnesium; cromoglycate; preemptive analgesia; drug interactions trigeminal pain; mast cell; neuroinflammation; nociception; magnesium; cromoglycate; preemptive analgesia; drug interactions

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MDPI and ACS Style

Srebro, D.; Dožić, B.; Vučković, S.; Savić Vujović, K.; Medić Brkić, B.; Dožić, I.; Srebro, M. The Interactions of Magnesium Sulfate and Cromoglycate in a Rat Model of Orofacial Pain; The Role of Magnesium on Mast Cell Degranulation in Neuroinflammation. Int. J. Mol. Sci. 2023, 24, 6241. https://doi.org/10.3390/ijms24076241

AMA Style

Srebro D, Dožić B, Vučković S, Savić Vujović K, Medić Brkić B, Dožić I, Srebro M. The Interactions of Magnesium Sulfate and Cromoglycate in a Rat Model of Orofacial Pain; The Role of Magnesium on Mast Cell Degranulation in Neuroinflammation. International Journal of Molecular Sciences. 2023; 24(7):6241. https://doi.org/10.3390/ijms24076241

Chicago/Turabian Style

Srebro, Dragana, Branko Dožić, Sonja Vučković, Katarina Savić Vujović, Branislava Medić Brkić, Ivan Dožić, and Milorad Srebro. 2023. "The Interactions of Magnesium Sulfate and Cromoglycate in a Rat Model of Orofacial Pain; The Role of Magnesium on Mast Cell Degranulation in Neuroinflammation" International Journal of Molecular Sciences 24, no. 7: 6241. https://doi.org/10.3390/ijms24076241

APA Style

Srebro, D., Dožić, B., Vučković, S., Savić Vujović, K., Medić Brkić, B., Dožić, I., & Srebro, M. (2023). The Interactions of Magnesium Sulfate and Cromoglycate in a Rat Model of Orofacial Pain; The Role of Magnesium on Mast Cell Degranulation in Neuroinflammation. International Journal of Molecular Sciences, 24(7), 6241. https://doi.org/10.3390/ijms24076241

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