Grasses, Volume 4, Issue 4 (December 2025) – 4 articles

Soil surfactants are essential tools for enhancing irrigation water efficiency and improving the quality and functionality of amenity turfgrass. They play a crucial role in sports turf management by reducing soil water repellency, which helps prevent dry spots, ensures even moisture distribution, and supports water conservation efforts. Most research on soil surfactants and amenity turfgrasses focuses on their effects on soil moisture, infiltration, and addressing localized dry spots during drought conditions, with limited studies on their impact under wet or saturated conditions. This study aimed to evaluate the impact of soil surfactants on the quality and health of turfgrass under wet conditions. Field studies were conducted over a span of five years, beginning in the USA in 2019 and continuing in Ireland from 2020 to 2023. The research in Ireland was conducted at three locations, each featuring different rootzones: a “push-up” green with loam soil, USGA-specification sand, and natural link sand. The site in the USA was a native loam soil. The study compared a commercial soil surfactant (ProWet Evolve; PWE) and a non-treated control (NT) in a randomized complete block design with four replications, with sequential applications starting in June and continuing until mid-September each year. The rootzone volumetric water content (VWC%), turfgrass quality, and normalized difference vegetation index (NDVI) were measured bi-weekly. Environmental conditions, with above-average precipitation each year, significantly influenced results. Although there were no significant or consistent differences in VWC% between the soil surfactant and NT-treated plots, turfgrass quality was significantly enhanced in the soil surfactant-treated plots and supported by higher NDVI values. Even in prolonged wet conditions with high VWC%, improved turfgrass quality was consistently observed in soil surfactant-treated plots across multiple locations in both countries over the five-year study period. Full article

High-quality playing surfaces enhance player experience and safety while serving as an appealing setting for spectators. Natural turfgrass provides optimal conditions at the beginning of the playing season but faces challenges under increasing field usage. Turfgrasses with high wear tolerance and quick recovery capacity are crucial for maintaining surface quality under intensive wear. Bermudagrass is the most used species in warm climates but needs winter overseeding in the transition zone. In Mediterranean climates, tall fescue (Schedonorus arundinaceus (Schreb.) Dumort, formerly Festuca arundinacea) has emerged as a promising species due to its tolerance to heat, drought, and salinity, alongside traits like deep rooting, shade adaptation, and wear resistance. The trial was conducted at the CeRTES experimental station in Rottaia, Pisa, Italy. Twenty-seven tall fescue cultivars and three cultivars of perennial ryegrass (Lolium perenne L.) were hand-seeded on 3 November 2022, at a rate of 43 g m⁻². The experimental design consisted of plots measuring 4.5 m² arranged in a randomized complete block design with three replications. The objective of the study is to evaluate the performance of twenty-seven cultivars of tall fescue with the aim of using the species in soccer fields with a permanent stand approach, with no need to manage spring and fall transitions. The field study encompasses determinations referring to the establishment stage, the maintenance at low cutting height stage (20 mm) and the subsequent stage of soccer use under different seasonal conditions (autumn, winter, and spring). Results showed that certain fescue cultivars, notably ‘Essential’, ‘Eyecandy’, and ‘FAG3/19-20208B’, exhibited quick establishment and adaptation to low cutting height (20 mm), and performed similarly to the reference ryegrasses ‘Gianna’ and ‘Mercitwo’ in terms of wear tolerance and recovery capacity across the three seasons. Moreover, most of the tested tall fescue cultivars performed well at a 20 mm mowing height, maintaining satisfactory quality and density. Among these, ‘Eyecandy’ and ‘Foxhound’ displayed finer leaf textures, comparable to those of the reference ryegrass. Full article

Warm-season grasses are the main source of feed in tropical and subtropical beef cattle production systems. The objective was to assess cattle preference among three warm-season grasses and explore its relationship with forage yield and plant structural traits. The three species were cultivated in 2 × 2 m plots using a completely randomized design. Cattle preference was evaluated in spring (December 2016 and 2017), summer (March 2017), and autumn (May 2017) using six Braford steers that grazed the plots for 4 h on two consecutive days. Pre-grazing forage yield, plant height, leaf-blade length, leaf-blade width, and the proportions of five leaf tissues at three leaf regions were measured at each date. Cattle preference was variable among the three species and evaluation dates. Paspalum atratum exhibited the highest pre-grazing forage yield, and constituted the tallest plants with the longest leaves during the summer. Urochloa brizantha showed the greatest proportion of vascular bundle sheath (17–30% at the midrib region, 25–31% at the interveinal region and 14–23% at the margin region) and P. atratum exhibited the greatest number of primary vascular bundle. Cattle preference was negatively correlated with the number of primary vascular bundle, pre-grazing forage yield, plant height and leaf-blade length. Full article

Napier grass (Cenchrus purpureus Schumach) is an important forage crop and livestock feed. However, its yield and quality in Kenya are often limited by Napier grass headsmut and stunt disease. Napier grass genetic improvements through mutation breeding and selection could avail cultivars with increased forage. This study investigated the response of embryogenic calli to different levels of colchicine in inducing polyploidy in the two germplasms of Napier grass; South africa and Bana grass. The experiments were carried out as a factorial experiment in a completely randomized design (CRD). The colchicine concentrations used were 0, 0.05, 0.1, and 0.2%, and the exposure durations were 24, 48, and 72 h. During the shoot regeneration stage, culturing explants on an MS medium (Murashige and Skoog) supplemented with 0.2 mg L⁻¹ Benzyl Adenine (BAP), 0.1 mg L⁻¹ dichlorophenoxyacetic acid (2, 4-D), and 0.1 mg L⁻¹ indole-3-butyric acid (IBA) was more suitable for shoot regeneration. Chromosome doubling was confirmed by genomic DNA and the stomata size and number. Culturing explants on an MS medium supplemented with 1 mg L⁻¹ IBA, 1 mg L⁻¹ 2, 4-D, and 0.5 mg L⁻¹ BAP was more suitable in inducing embryogenic calli in both genotypes. Polyploidy results revealed that a 0.1% concentration of colchicine with two days of treatment established the maximum number of octoploid plantlets induced in vitro, while a 0.2% concentration was very toxic. The stomata size and number of derived octoploid plantlets were bigger with a lower density, a shorter plant height, and a smaller stem diameter, and despite being the first to produce tillers, they were significantly higher than their progenitors. Induced mutants also had a significantly higher number of chromosomes and showed different band patterns and distances during gel electrophoresis. However, we recommend the use of flow cytometry to confirm the ploidy level. The superior mutant plantlets can be selected and recommended for characterization across representative agro-ecologies for large-scale production and used in Cenchrus purpureus breeding programs in Kenya and its environments. Full article