Molecular Detection of Human Pathogenic Gastric Helicobacter Species in Wild Rabbits (Oryctolagus cuniculus) and Wild Quails (Coturnix coturnix)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Samples Collection
2.2. DNA Extraction and PCR Conditions and Sequencing
3. Results and Discussion
3.1. Wild Rabbits
3.2. Wild Quails
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A
Animals (n = 18) Samples (n = 30) | H. bizzozeronii | Accession nr./ Homology * | H. felis | Accession nr./ Homology | H. salomonis | Accession nr./ Homology | H. pylori | Accession nr./ Homology | H.suis | Accession nr./ Homology | H. ailurogastricus | Accession nr./ Homology | H. heilmannii | Accession nr./ Homology |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
WR1 | Fundus | - | - | - | - | - | - | - | ||||||
Corpus | - | - | - | - | - | - | - | |||||||
WR2 | Fundus | - | + | FQ670179 99.35% | - | - | - | - | - | |||||
Corpus | - | - | - | - | - | - | - | |||||||
WR3 | Fundus | - | - | - | - | - | - | - | ||||||
Corpus | + | FR871757 99.42% | - | - | - | - | - | - | ||||||
WR4 | Fundus | - | - | + | AJ130882 100% | - | - | - | - | |||||
Corpus | - | - | + | AJ130882 100% | - | - | - | - | ||||||
WR5 | Fundus | - | - | + | AJ130882 100% | - | - | - | - | |||||
Corpus | + | FR871757 100% | + | AJ130882 100% | - | - | - | - | ||||||
WR6 | Fundus | - | - | - | - | - | - | - | ||||||
Corpus | + | FR871757 95.08% | - | + | AJ130882 100% | - | - | - | - | |||||
WR7 | Fundus | - | - | + | AJ130882100% | - | - | - | - | |||||
Corpus | - | - | + | AJ130882 100% | - | - | - | - | ||||||
WR8 | Fundus | + | AF508003 97.11% | - | + | AJ130882 100% | - | - | - | - | ||||
Corpus | + | FR871757 100% | - | + | AJ130882 100% | - | - | - | - | |||||
WR9 | Fundus | - | - | + | AJ130882 100% | - | - | - | - | |||||
Corpus | - | - | + | AJ130882 100% | - | - | - | - | ||||||
WR10 | Fundus | - | - | + | AJ130882 100% | - | - | - | - | |||||
Corpus | - | + | JQ736360 100% | + | AJ130882 100% | - | - | - | - | |||||
WR11 | Fundus | - | - | + | AJ130882 100% | - | - | - | - | |||||
Corpus | + | AF508003 100% | - | + | AJ130882 100% | - | - | - | - | |||||
WR12 | Fundus | - | - | + | AJ130882 100% | - | - | - | - | |||||
Corpus | - | - | - | - | - | - | - | |||||||
Q1 | Gizzard | - | - | + | AJ130882 100% | - | - | - | ||||||
Q2 | Gizzard | - | - | + | AJ130882 100% | - | - | - | ||||||
Q3 | Gizzard | - | - | + | AJ130882 100% | - | - | - | ||||||
Q4 | Gizzard | + | FR871757 100% | - | + | AJ130882 100% | - | - | - | |||||
Q5 | Gizzard | + | FR871757 100% | + | AF116580 97.12% | + | AJ130882 100% | - | - | - | ||||
Q6 | Gizzard | - | - | + | AJ130882 100% | + | CP024947 100% | - | - |
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Helicobacter Species | Primer | Sequence | Target Gene | Amplicon Size | Thermal Cycle Conditions | Helicobacter Species Culture Used as Positive Control | ||
---|---|---|---|---|---|---|---|---|
Nr. Cycles | Temp. (C) | Time | ||||||
H. suis | BFHsuis_F1 | AAA ACA MAg gCg ATC gCC CTg TA | ureA | 150 bp | 40 | 95 60 72 | 20 s 30 s 30 s | HS1 |
BFHsuis_R1 | TTT CTT CgC CAg gTT CAA AgC g | ureA | ||||||
H. heilmannii | Hh-IceA-FWQ | gTT TCC AAC CAA AAg ACT CA | iceA | 135 bp | 30 | 94 55 72 | 30 s 30 s 30 s | ASB1.4 |
Hh-IceA-RVQ | ATT gCC TAg Agg TTg TgT Tg | iceA | ||||||
H. ailurogastricus | Ha-LpsA-FWQ | CTT gAg TAC ggC gAT gTC AAT | lpsA | 136 bp | 30 | 94 55 72 | 30 s 30 s 30 s | ASB7.1 |
Ha-LpsA-RVQ | ggg gAA AAA TgT gCT TgA AgT | lpsA | ||||||
H. salomonis | Hsal_FQ_PAR | CTC TTA TgA gTT ggA CTT ggT gCT CAC CAA T | ureAB | 91 bp | 45 | 94 61 72 | 30 s 30 s 1 min | R1051 |
Hsal_RQ_PAR | TTT gCC ATC TTT AAT TCC AAT gTC ggC | ureAB | ||||||
H. felis | BFHfel_F2 | gCT ggT ggC ATC gAT ACg CAT | ureAB | 154 bp | 45 | 94 60 72 | 30 s 30 s 1 min | CS1 |
BFHfel_R2 | TTT TTA gAT TAg CgC gTC Cgg gA | ureAB | ||||||
H. bizzozeronii | Hbizz_FQ_PAR | CCA ACA AAT CCC CAC AgC ATT TgC CAg | ureAB | 91 bp | 45 | 94 58 72 | 1 min 1 min 1 min | R1053 |
Hbizz_RQ_PAR | AgT CCC ATC AgC Wgg WCC TgT TCC CCC AC | ureAB | ||||||
H. pylori | BFHpyl_F1 | AAA gAg CgT ggT TTT CAT ggC g | ureAB | 217 bp | 45 | 94 59 72 | 30 s 30 s 1 min | 26695 |
BFHpyl_R1 | ggg TTT TAC CgC CAC CgA ATT TAA | ureAB |
Wild Rabbits (n = 12) | Fundus (n = 12) | Corpus (n = 12) | |
---|---|---|---|
H. salomonis | 9 (75.0%) | 8 (66.7%) | 8 (66.7%) |
H. bizzozeronii | 5 (41.7%) | 1 (8.3%) | 5 (41.7%) |
H. felis | 2 (16.7%) | 1 (8.3%) | 1 (8.3%) |
Wild Quails (n = 6) Gizzard | |
---|---|
H. salomonis | 6 (100%) |
H. bizzozeronii | 2 (33.3%) |
H. felis | 1 (16.7%) |
H. pylori | 1 (16.7%) |
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Cortez Nunes, F.; Letra Mateus, T.; Teixeira, S.; Barradas, P.F.; Gärtner, F.; Haesebrouck, F.; Amorim, I. Molecular Detection of Human Pathogenic Gastric Helicobacter Species in Wild Rabbits (Oryctolagus cuniculus) and Wild Quails (Coturnix coturnix). Zoonotic Dis. 2021, 1, 42-50. https://doi.org/10.3390/zoonoticdis1010005
Cortez Nunes F, Letra Mateus T, Teixeira S, Barradas PF, Gärtner F, Haesebrouck F, Amorim I. Molecular Detection of Human Pathogenic Gastric Helicobacter Species in Wild Rabbits (Oryctolagus cuniculus) and Wild Quails (Coturnix coturnix). Zoonotic Diseases. 2021; 1(1):42-50. https://doi.org/10.3390/zoonoticdis1010005
Chicago/Turabian StyleCortez Nunes, Francisco, Teresa Letra Mateus, Sílvia Teixeira, Patrícia F. Barradas, Fátima Gärtner, Freddy Haesebrouck, and Irina Amorim. 2021. "Molecular Detection of Human Pathogenic Gastric Helicobacter Species in Wild Rabbits (Oryctolagus cuniculus) and Wild Quails (Coturnix coturnix)" Zoonotic Diseases 1, no. 1: 42-50. https://doi.org/10.3390/zoonoticdis1010005
APA StyleCortez Nunes, F., Letra Mateus, T., Teixeira, S., Barradas, P. F., Gärtner, F., Haesebrouck, F., & Amorim, I. (2021). Molecular Detection of Human Pathogenic Gastric Helicobacter Species in Wild Rabbits (Oryctolagus cuniculus) and Wild Quails (Coturnix coturnix). Zoonotic Diseases, 1(1), 42-50. https://doi.org/10.3390/zoonoticdis1010005