The First Finding of Francisella tularensis subsp. mediasiatica in Krasnoyarsk Territory, Siberia, and an Update of the Subspecies Genetic Diversity
Abstract
:1. Introduction
2. Results
2.1. K-334 Strain Isolation and Subspecies Determination
2.2. Whole-Genome Sequencing (WGS) and Phylogenetic Analysis
3. Discussion
4. Materials and Methods
4.1. Strains
4.2. Bacterial Cultures
4.3. DNA Preparation and PCR Analysis of Species and Subspecies
4.4. Sequencing
4.5. WGS Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | Isolation Date | Source | Isolation Area |
---|---|---|---|
A-99 | 23 May 2013 | ticks Haemaphysalis concinna (caught 11 April 2013) | Altai Republic, Maiminsky district, near Aleksandrovka village |
A-188 | 26 June 2013 | ticks H. concinna (caught 14 May 2013) | Altai Republic, Choysky district, natural landmark Ashpanak |
A-82 | 25 May 2014 | ticks Dermacentor silvarum (caught 20 May 2014) | Altai Republic, Choysky district, near Uba river |
A-84 | 25 May 2014 | ticks D. silvarum (caught 20 May 2014) | Altai Territory, Sovietskiy district, near Kolovo village |
A-116 | 20 May 2014 | ticks D. silvarum (caught 12 May 2014) | Altai Territory, Altaiskiy district |
A-137 | 29 May 2014 | ticks H. concinna (caught 16 May 2014) | Altai Republic, Choysky district, near Paspaul village |
A-139 | 17 June 2014 | ticks H. concinna (caught 20 May 2014) | Altai Republic, Choysky district, near Ashpanak river |
A-142 | 10 June 2014 | ticks H. concinna (caught 20 May 2014) | Altai Republic, Choysky district, near Uba river |
A-174 | 23 May 2014 | ticks H. concinna (caught 16 May 2014) | Altai Republic, Choysky district, near Uba river |
A-178 | 26 May 2014 | ticks D. silvarum (caught 20 May 2014) | Altai Republic, Choysky district, near Karakokshu village |
A-77 | 23 May 2015 | ticks H. concinna (caught 27 April 2015) | Altai Republic, Maiminsky district, near the Bakala brook |
A-87 | 14 May 2015 | ticks H. concinna (caught 29 April 2015) | Altai Republic, Maiminsky district |
A-196 | 21 May 2015 | ticks H. concinna (caught 12 May 2015) | Altai Republic, Choysky district, near Paspaul village |
B-7175 (A-554) | 2011 | ticks H. concinna | Altai Republic, Eltsovskiy district, Martynovo village |
B-7176 (A-678) | 2011 | ticks Ixodes persulcatus | Altai Republic, Pervomayskiy district, Pokrovka village |
B-7177 (A-823) | 2011 | Northern red-backed vole (Clethrionomys rutilus) | Altai Republic, Shelabolikhinskiy district, Molokovo village |
K-334 | 30 July 2021 | ticks I. persulcatus or H. concinna * (caught 20 May 2021) | Krasnoyarsk region, Karatuzsky District, Western Sayan mountain-taiga zone |
120 | ≤1968 | unknown | Kazakhstan |
117 | 1960 | ticks Hyalama sp. | Kazakhstan |
60(B)57 | 1960 | unknown | Karakalpakstan (Uzbekistan) |
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Timofeev, V.; Bakhteeva, I.; Mokrievich, A.; Vakhrameeva, G.; Gritskova, E.; Anisimov, Y.; Rozhdestvensky, E.; Bazarova, G.; Zhumakaev, R.; Dyatlov, I.; et al. The First Finding of Francisella tularensis subsp. mediasiatica in Krasnoyarsk Territory, Siberia, and an Update of the Subspecies Genetic Diversity. Bacteria 2022, 1, 242-249. https://doi.org/10.3390/bacteria1040018
Timofeev V, Bakhteeva I, Mokrievich A, Vakhrameeva G, Gritskova E, Anisimov Y, Rozhdestvensky E, Bazarova G, Zhumakaev R, Dyatlov I, et al. The First Finding of Francisella tularensis subsp. mediasiatica in Krasnoyarsk Territory, Siberia, and an Update of the Subspecies Genetic Diversity. Bacteria. 2022; 1(4):242-249. https://doi.org/10.3390/bacteria1040018
Chicago/Turabian StyleTimofeev, Vitalii, Irina Bakhteeva, Alexander Mokrievich, Galina Vakhrameeva, Elena Gritskova, Yuriy Anisimov, Evgeny Rozhdestvensky, Galina Bazarova, Rostislav Zhumakaev, Ivan Dyatlov, and et al. 2022. "The First Finding of Francisella tularensis subsp. mediasiatica in Krasnoyarsk Territory, Siberia, and an Update of the Subspecies Genetic Diversity" Bacteria 1, no. 4: 242-249. https://doi.org/10.3390/bacteria1040018