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Article

Trace Metal Concentration in Beach-Cast Seaweeds from Southeastern Brazil Indicates the Legacy of the Mining Industry

by
Thiago Holanda Basilio
1,2,*,
Bianca Rodrigues Ramalhete Nunes
2,
Angélica Elaine Neto
2,
Daisa Hakbart Bonemann
3,
Danielle Tapia Bueno
3,
Mutue T. Fujii
4,
Iago Alonso
5,
Ana Teresa Lima
6,
Weber Adão Rodrigues Junior
7,
Eduardo Schiettini Costa
7 and
Renato Rodrigues Neto
7
1
Ministry of Fisheries and Aquaculture—MPA, Superintendence of Fisheries and Aquaculture of Sergipe, Aracaju 49065-310, SE, Brazil
2
Federal Institute of Education, Science and Technology of Espírito Santo-Ifes-Piúma, Piúma 29285-000, ES, Brazil
3
Laboratory of Innovation and Solutions in Chemistry, Bioforensics Research Group, Federal, University of Pelotas, Pelotas 96010-900, RS, Brazil
4
Biodiversity Conservation Center, Institute of Environmental Research—IPA, São Paulo 04301-012, SP, Brazil
5
Postgraduate Program in ‘Vegetal Biodiversity and Environment’, Institute of Environmental Research—IPA, São Paulo 04301-012, SP, Brazil
6
Department of Environmental and Resource Engineering, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark
7
Laboratory of Environmental Geochemistry and Marine Pollution, Federal University of Espírito Santo—UFES, Vitória 29075-910, ES, Brazil
*
Author to whom correspondence should be addressed.
Phycology 2026, 6(2), 44; https://doi.org/10.3390/phycology6020044
Submission received: 31 March 2026 / Revised: 16 April 2026 / Accepted: 16 April 2026 / Published: 21 April 2026
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Abstract

Seaweeds are photosynthetic organisms with ecological, social, and economic significance, and they serve as effective bioindicators in marine ecosystems. This study assessed trace element concentrations in beach-cast seaweeds collected from four beaches along the Espírito Santo coast in southeastern Brazil—an area impacted by mining-related contamination. Samples of Zonaria tournefortii (J.V. Lamouroux) Montagne and Sargassum natans (Linnaeus) Gaillon, gathered during low tide (July–August 2022), were analyzed for 15 elements. Statistical analysis using the Kruskal–Wallis test revealed significant interspecific differences in the accumulation of several metals. Aluminum (Al), iron (Fe), and magnesium (Mg) were the most abundant (>100 mg/kg), while minor elements (<100 mg/kg) included barium (Ba), arsenic (As), zinc (Zn), vanadium (V), nickel (Ni), chromium (Cr), copper (Cu), lead (Pb), cobalt (Co), cadmium (Cd), silver (Ag), and mercury (Hg). Elemental profiles exceeded those reported in other global regions and closely resembled iron ore tailings. Most elements had relatively higher concentrations on the beaches of Imigrantes, in the north of the state. These findings are the first for beach-cast seaweeds in this region, suggesting that this contamination indicates the legacy of the mining industry from southeastern Brazil.

Graphical Abstract

1. Introduction

Coastal regions worldwide are experiencing significant environmental stress and are under pressure from various forms of anthropogenic activity [1,2]. These processes may chemically contaminate rivers and oceans. Some of the most harmful groups are metals and other elements, such as arsenic, known as trace elements in the oceans because of their low levels. Their high concentrations can negatively influence biota, impacting organisms such as fish and invertebrates [3]. A significant example of an environmental disaster with global repercussions is the contamination of the Brazilian southeast coast with iron ore tailings (IOTs). This material was released due to the collapse of the Fundão tailings dam in Mariana, Minas Gerais, Brazil, in 2015. This event released approximately 45 million cubic meters of iron mining waste into the Doce River, which spans the states of Minas Gerais and Espírito Santo (ES), Brazil [4]. After flowing about 600 km in 16 days, the IOTs reached the ocean in a region with elevated biodiversity and different ecosystems like estuaries, mangroves and corals. The biogeochemistry of the area was altered by the input of nutrients, hydrocarbons and metals [4]. The disaster was so severe that the dissolved iron flux of the Doce River was calculated to be up to 5% of the global riverine flux [5]. Consequently, phytoplankton, zooplankton, benthos, and fish were negatively impacted. Besides its rich biodiversity, the region is regarded as one of the greatest calcium carbonate banks in the world [6], where macroalgae have an essential function in the food chain.
Macroalgae occur in all oceans and play a significant role in primary productivity [7]. They can be divided into three main groups: Chlorophyta (green), Phaeophyceae (brown), and Rhodophyta (red). They have evolved to live under varying environmental stress conditions, such as changes in temperature, salinity, environmental pollutants, or exposure to UV radiation. Natural phenomena associated with hydrodynamics can cause macroalgae to detach from their substrate in coastal regions, leading to deposition along the shoreline. The resulting algal material deposited on beaches is known as beach-cast seaweeds [8]. This phenomenon is becoming increasingly frequent [8,9,10], and management is crucial in order to deal with their vast biomass, which has become more common in the last decade, similar to what has been happening with the formation of the Great Atlantic Sargassum Belt—GASB—extending from West Africa to the Caribbean, reaching a maximum of 20 million wet tons of seaweed in June 2018 [11] and affecting many beaches in several countries.
The richness and abundance of beach-cast seaweeds have sparked economic, social, and environmental interest across various sectors of society, although often associated with negative economic impacts on industries such as tourism and artisanal fishing, in addition to requiring ongoing cleaning efforts on affected beaches [12]. This issue is compounded by the foul odor resulting from decomposition [8] and the production of toxic gases—H2S and ammonia [13]. On the other hand, they are considered a valuable, yet underutilized and underestimated, biomass that should be regarded as a sustainable source of bioactive compounds [8,9,10,14,15,16,17]. Harb and Chow [18] showed that this has been the focus of studies regarding animal feed, bioactivity, bioenergy, biosorption of metals, chemical composition, ecology, fertilizer, human food, taxonomy and abundance.
Macroalgae are widely recognized for their ability to be used as contaminant bioindicators [19]. They can adsorb different chemicals, including petroleum hydrocarbon compounds [20] and trace elements [21]. Within this context, the main goal of this research is to verify if metals found in two species of beach-cast seaweeds—Sargassum natans (Linnaeus) Gaillon and Zonaria tournefortii (J.V. Lamouroux) Montagne—collected from four representative beaches from the Espírito Santo (ES) coast in the southeast of Brazil have a similar pattern to that found in the primary source of recent pollution, the IOTs from the Fundão dam burst in 2015.

2. Materials and Methods

2.1. Study Area

The beach-cast seaweeds were collected during July and August 2022 in southeastern Brazil, in the north and south regions of Espírito Santo (ES), Brazil. In the north region, the collections took place in the municipalities of Aracruz (Barra do Sahy beach and Imigrantes beach) and Fundão (Enseada das Garças beach). In the south region, the collections were carried out in the municipality of Itapemirim (Itaoca beach) (Figure 1).
The municipalities were selected due to the large biomass of seaweed deposited on the beach, available throughout the year [10]. These beaches possess high tourist accessibility, the presence of industrial companies, proximity to ports and intense urbanization. In addition, navigation, fishing and tourist waste pollute the coastal aquatic environment of these regions. The beaches of the north coast are closer to the mouth of the Rio Doce. In some places, such as Itapemirim and Aracruz, there is intense urban pressure, while in others, such as Imigrantes and Enseada das Garças, the level of conservation is higher [22]. These beaches are part of the Federal Conservation Unit, the Environmental Protection Area of the Costa das Algas [23] (Table 1).

2.2. Collection of Field Samples

Sampling was carried out during low-tide periods when the probability of the presence of beach-cast seaweeds is higher, as they remain on the sand for longer. The project was authorized by the Biodiversity Authorization and Information System (SISBIO) under No. 78465-1/85335. A field protocol adapted from [8,24] was employed to collect macroalgae. Two 50 m transects were established to delimit the collection areas. The first transect was positioned near the waterline, in a lower intertidal region, while the second was placed near the sandbank vegetation, in an upper intertidal region. In each transect, macroalgae samples were collected randomly in three quadrants (Q1, Q2 and Q3) with an area of 1 × 1 m. Two species of brown macroalgae were selected, Zonaria tournefortii and Sargassum natans, due to their abundance on the studied beaches [10]. The macroalgae were collected by hand with nitrile gloves and placed in plastic bags labeled with the date and location of collection. They were then transported in coolers and immediately stored at −10 °C at the Fishing and Aquaculture Extension Laboratory of the Federal Institute of Education (Ifes) Piúma Campus from Brazil, to ensure the integrity of the collected material.

2.3. Sample Preparation

The cleaning of equipment, such as trays, pestles, crucibles, Petri dishes, sieves, and Eppendorf tubes, was initially carried out with running water to remove organisms and sediments. Then, these materials were washed with nitric acid (3%) and subsequently with ultrapure water in three consecutive cycles (this procedure was performed one day before the start of sample processing for metal analysis). Next, the macroalgae were carefully selected and distributed in plastic trays to avoid contact with metallic utensils, which could interfere with the analysis results.
The macroalgae were then washed with nitric acid (3%) and distilled water, repeating the process three times. After washing, the samples were weighed, and the data were recorded in a control spreadsheet. The macroalgae were then transferred to a drying oven, maintained at a temperature between 50 and 60 °C, where they remained for 72 h until they were completely dry. After drying, the macroalgae were weighed again, ground into a fine powder, and sieved. The powdered material was stored in Eppendorf tubes containing 1 g of sample for analysis.

2.4. Data Analysis

The distribution of the data was checked with the Shapiro−Wilk test. As some elements presented non-normal distribution, the data are shown as median, with their interquartile range. The differences between the transects and the species were verified with the Mann–Whitney U test. As the transects did not show differences for the metals, the data were combined and used for each beach. The same was carried out for the data from the samples of July and August, as these months are not enough to represent a temporal variation. The differences among the data of the beaches were tested with the Kruskal–Wallis test. All the analyses were performed using three replicates.
For partial metal analysis, the EPA 3052 method was used. Briefly, about 0.25 g of the sample was digested using nitric acid (Merck, Darmstadt, Germany, sub-boiling) and hydrogen peroxide (30%) in a 9:2 ratio and heated in a microwave oven (MARS 5 X-Press, CEM corporation, Carolina do Norte, NC, USA) following these parameters: 1st temperature ramp from 25 °C to 175 °C over 5:30 min and the 2nd ramp from 25 °C to 175 °C over 4:30 min, both at 1600 W power. Then, the solution was cooled and filtered using a quantitative filter, followed by analysis using inductively coupled plasma mass spectrometry (ICP-MS).
The elements aluminum (Al), vanadium (V), chromium (Cr), manganese (Mn), iron (Fe), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), arsenic (As), silver (Ag), cadmium (Cd), barium (Ba), mercury (Hg) and lead (Pb), were determined by ICP-MS (7500cx, Agilent Technologies, Santa Clara, CA, USA). The method used was described in EPA 6020B (USEPA) adapted from [25]. Quantification was performed using an analytical calibration curve ranging from 0.1 to 40 ppb, using a multi-element standard, with R2 > 0.99. A multi-element internal standard (Internal Standard Mix—Bi, Ge, In, Li, Sc, Tb and Y, Agilent Technologies) was used for correction of possible fluctuations in the measurements of the signals from the elements analyzed. Spiked samples were measured to identify matrix effects and determine the recovery of an analyte or the selectivity of a method. Recoveries of the spiked samples ranged from 81.1% (Ni) to 101.9% (Ba). Limits of detection and quantification (LD and LQ, respectively) were calculated using 3.3 times and 10 times the quotient of analytical curve angular coefficient and the standard deviation of 10 blanks, respectively (Table 2).
Principal Component Analysis (PCA) was performed to explore multivariate patterns. Prior to analysis, the datasets for both species were combined into a single matrix, and only numerical variables representing element concentrations were retained. PCA was conducted using the function (PCA) from the FactoMineR package in R (version 4.5.0).
The variables were automatically centered before analysis and the decomposition was performed using singular value decomposition (SVD). The first three principal components were used to generate a three-dimensional visualization of the samples. The 3D scatter plot was produced using the plotly package (R, version 4.5.0).

3. Results

The concentrations of 15 trace elements were quantified in two species of beach-cast seaweeds, S. natans and Z. tournefortii, collected from four distinct coastal sites (Figure 2 and Figure 3). Statistical analysis revealed significant interspecific differences in the accumulation of several metals. Specifically, Z. tournefortii exhibited significantly higher concentrations of iron, chromium, vanadium and aluminum (p < 0.001), whereas S. natans showed higher levels of cobalt and lead (p < 0.038).
Despite these statistically significant differences, the overall pattern of metal accumulation was remarkably similar between the two species. The median concentrations followed a consistent decreasing order: Al > Fe > Mn > Ba > As > Zn > V > Cu > Cr > Ni > Pb > Co > Cd > Hg > Ag. The most notable divergence between the species was observed in the relative positions of chromium and nickel: in Z. tournefortii, Cr concentrations exceeded those of Ni, while the opposite trend was observed in S. natans. These findings suggest that although species-specific uptake mechanisms may influence the accumulation of certain elements, the general profile of metal distribution remains largely conserved across both macroalgae.
Samples to Z. tournefortii revealed statistically significant differences in the concentrations of seven metals across four sampling sites: Barra do Sahy, Enseada das Garças, Imigrantes, and Itaoca. The metals exhibiting significant variation were manganese (Mn), arsenic (As), copper (Cu), nickel (Ni), lead (Pb), cobalt (Co), and silver (Ag) (p ≤ 0.024). To identify which specific beach comparisons accounted for these differences, Dunn’s post hoc test with Bonferroni correction was conducted. For Mn, a significant difference was observed between Barra do Sahy and Imigrantes (p = 0.0097). As concentrations differed significantly between Itaoca and Imigrantes (p = 0.0161). Cu levels were significantly different between Barra do Sahy and Itaoca, and between Enseada das Garças and Itaoca (p ≤ 0.0009). Ni concentrations varied significantly between Barra do Sahy and Imigrantes, and between Barra do Sahy and Itaoca (p ≤ 0.0161). Pb levels were significantly lower in Barra do Sahy compared to Enseada das Garças and Itaoca (p ≤ 0.0117). For Co, Itaoca showed significantly higher concentrations compared to Barra do Sahy, Enseada das Garças, and Imigrantes (p ≤ 0.0134). Finally, Ag concentrations were significantly different between Itaoca and Barra do Sahy (p = 0.0204). Overall, Itaoca beach exhibited distinct metal profiles for six elements (As, Cu, Ni, Pb, Co, and Ag), indicating localized environmental influences affecting metal accumulation in Z. tournefortii.
Samples of S. natans revealed significant spatial variation in the concentrations of 11 trace elements across four beaches: Barra do Sahy, Enseada das Garças, Imigrantes, and Itaoca. The elements showing significant differences were aluminum (Al), iron (Fe), manganese (Mn), barium (Ba), vanadium (V), copper (Cu), chromium (Cr), nickel (Ni), cobalt (Co), lead (Pb), and silver (Ag) (p < 0.001). For aluminum (Al), the test indicated significant variation between Imigrantes and Barra do Sahy, Imigrantes and Enseada das Garças, and Itaoca and Enseada das Garças (p ≤ 0.0120). Iron (Fe) also varied significantly (p < 0.001), with Imigrantes differing from Barra do Sahy and Enseada das Garças, while Itaoca differed from Barra do Sahy and Enseada das Garças (p ≤ 0.0104). Manganese (Mn) showed highly significant variation between Imigrantes and both Barra do Sahy and Enseada das Garças, and between Itaoca and Barra do Sahy (p ≤ 0.0185). Although barium (Ba) showed overall significance (p = 0.04), no pairwise comparisons reached statistical significance in Dunn’s test. Vanadium (V) also exhibited significant differences among beaches (p < 0.001), though specific pairwise results were not detailed. These findings suggest that S. natans accumulates metals in patterns influenced by local environmental conditions, with Imigrantes and Itaoca beaches showing the most distinct profiles for multiple elements.
Further statistical analysis revealed significant pairwise differences in metal concentrations among the beaches sampled for S. natans. Specifically, significant differences were observed between Imigrantes and Barra do Sahy, Itaoca and Barra do Sahy, and Itaoca and Enseada das Garças (p < 0.0071). For copper (Cu), although the Kruskal–Wallis test indicated significant variation among beaches (p = 0.02), none of the pairwise comparisons reached statistical significance. Chromium (Cr) showed significant overall variation, between Itaoca and Barra do Sahy, and between Itaoca and Enseada das Garças (p < 0.0104). Nickel (Ni) also varied significantly across beaches, with Imigrantes exhibiting lower concentrations than Barra do Sahy. Lead (Pb) showed highly significant variation (p < 0.0041) between Imigrantes and Barra do Sahy, and between Itaoca and Barra do Sahy (p ≤ 0.0061). For cobalt (Co), significant differences were found between Imigrantes and Barra do Sahy, and between Imigrantes and Enseada das Garças (adjusted p ≤ 0.0140). Silver (Ag) also showed significant variation between Itaoca and Barra do Sahy, and between Itaoca and Enseada das Garças (p ≤ 0.0107). Overall, the results suggest that the beaches of Imigrantes and Itaoca tend to exhibit relatively higher concentrations of several trace metals, indicating localized environmental factors influencing metal accumulation in S. natans. In summary, most elements had relatively higher concentrations in the beaches of Imigrantes and Itaoca.
The 3D Principal Component Analysis (PCA) presented in Figure 4 illustrates the variation in metal concentrations across the two macroalgae species. The first three principal components (PC1, PC2, and PC3) collectively explain 57.94% of the total variance in the dataset, indicating a moderate dimensional reduction with meaningful interpretability. PC1 accounts for the largest portion of the variance (29.69%), capturing the primary gradient of metal concentration differences among samples. PC2 contributes 15.83% of the variance, representing secondary patterns of variation, while PC3 explains additional subtle differences not captured by the first two components. The spatial distribution of samples in the 3D PCA plot suggests distinct clustering patterns between species, reflecting underlying differences in metal accumulation profiles.

4. Discussion

The species analyzed in this study represent the most frequent brown macroalgae along the coast of Espírito Santo (ES), with Z. tournefortii being the dominant one [10]. Notably, there are no prior reports in the literature addressing metal concentrations in Z. tournefortii, highlighting the novelty of this investigation. In contrast, trace metal accumulation in S. natans (often referred to as S. vulgare) has been previously examined, although existing studies have not focused on drift samples [26,27]. Comparative data from the coast of Ceará, Brazil [26], indicate that during the rainy season, S. natans exhibits the following order of decreasing metal concentrations: Al > Fe > Mn > As > Ba > Zn > Cr > Cu > Co > Hg > Cd, with V, Ni, and Pb below the limit of detection (LOD) for the elements common to this study (Table 3). In the dry season, overall concentrations are lower, and the order shifts to Al > Fe > Mn > As > Ba > Hg > V > Cr > Co > Cd, with Cu, Ni, Pb, and Zn below the LOD [26]. In comparison, S. natans samples from the present study showed elevated concentrations of Ag, while levels of V, Ni, Pb, Ba, and Cu were lower than those reported for the Ceará coast. The remaining elements fell within a similar concentration range, suggesting regional variability in metal accumulation influenced by environmental and hydrodynamic conditions.
The concentrations of Ba, Ni, Hg, and Cd in this study were higher than those reported for the same species (reported as S. vulgare) in the Syrian Sea [28], while Pb levels were comparable. The remaining elements exhibited lower concentrations relative to the Syrian dataset. When compared to a more pristine environment, such as the Madeira Archipelago [27], our results from the Espírito Santo (ES) coast showed lower concentrations of Zn and Cd. In contrast, Cu, Cr, Ni, Pb, and Hg were found at higher levels in the ES samples (Table 3). These differences suggest that regional environmental conditions, anthropogenic inputs, and hydrodynamic factors may significantly influence the bioaccumulation of trace metals in S. natans.
Seasonal variation in metal concentrations observed in macroalgae is largely influenced by environmental processes such as the leaching of sediments and continental rocks during the rainy season, as reported by [26]. This influx of dissolved metals contributes to elevated concentrations during wetter periods. In contrast, the dry season is characterized by reduced metal input and increased macroalgal growth rates, which may dilute intracellular metal concentrations. Consequently, the metal levels detected in the present study could have been higher if sampling had occurred during the rainy season. Despite these seasonal fluctuations, a consistent pattern of metal distribution is evident across locations and species. Aluminum (Al), iron (Fe), and manganese (Mn) consistently dominate the elemental profile, followed by intermediate concentrations of zinc (Zn), chromium (Cr), and arsenic (As), while other elements occur at lower levels. This distribution pattern is not only observed in S. natans and Z. tournefortii but also aligns with findings from other macroalgal species in different regions, suggesting a conserved physiological or ecological mechanism governing metal uptake [29].
In 2015, the catastrophic failure of the Fundão dam released over 45 million m3 of iron ore tailings (IOTs) into the Doce River basin, causing extensive environmental damage to both the river and the adjacent coastal ecosystems [30,31]. The IOTs significantly altered the region’s biogeochemistry and biodiversity, and recent studies confirm that contamination persists nearly a decade later [32,33,34]. Notably, the elemental composition of the iron ore tailings shows a distribution pattern similar to that observed in the macroalgae analyzed in this study. Segura et al. [35] reported the following decreasing order of metal concentrations in residual water contaminated by IOTs: Fe > Al > Mn > Cr > Ba > Zn > Cu > Pb > Ni > As > Co > Ag > Hg > Cd. Sa et al. [36], analyzing solid IOTs from the burst dam, found a comparable sequence: Fe > Al > Mn > Ba > V > Zn > Pb ≈ Cu > Cr ≈ Ni > As > Cd. The primary discrepancy between these two studies lies in the relative abundance of Cr. When compared to the macroalgae results presented here, four subtle differences emerge: Fe and Al concentrations are relatively higher in IOTs; V and Zn show reversed positions; Pb is lower in IOTs; and As is notably more concentrated in the seaweed samples. This last point is particularly relevant, as Sargassum species are known to accumulate arsenic even in pristine environments [37], and the Espírito Santo coast has been identified as one of the most chronically arsenic-contaminated regions in Brazil [33,38].
For further comparison, Nassa et al. [39] investigated metal concentrations in Padina gymnospora (Kützing) Sonder, another brown macroalga, in Espírito Santo Bay—a site historically impacted by waste discharge from an iron ore pellet processing plant for over two decades. This area lies geographically between the northern and southern sampling sites of the present study. Their findings revealed significantly elevated levels of Al, Fe, and Zn (10×, 3×, and 3× higher, respectively) compared to the current results. Manganese (Mn) was lower (2–3×), while Cr, Pb, Ni, Cu, and Cd showed similar concentrations. Based on these data, the authors of [39] concluded that Fe, Al, and Cu could serve as reliable indicators of ore-related pollution. Given the similarity in concentrations of other elements between their control site and our study area—despite being only a few kilometers apart—it is plausible that their reference site may also be subject to contamination.
The spatial variation in metal concentrations observed among the sampled beaches reflects the combined influence of anthropogenic and natural factors, particularly seasonal rainfall patterns and associated runoff that mobilize and transport metals into coastal environments [40,41]. Elevated concentrations of trace elements at specific sites suggest the presence of localized pollution sources, likely linked to industrial and urban effluent discharges, which are further intensified during periods of heavy rainfall [42]. Among the beaches studied, Itaoca consistently exhibited the highest metal concentrations when compared to Imigrantes, Enseada das Garças, and Barra do Sahy. This pattern is likely attributable to Itaoca’s high degree of urbanization and its proximity to the Itapemirim River, which exerts a strong influence on the local marine environment. The Itapemirim River basin spans approximately 6181 km2 and supports a population of over 522,000 inhabitants. Importantly, this basin hosts the largest industrial processing park for ornamental stones in Brazil, contributing significantly to global stone product output. The soils and sediments within the Itapemirim basin are notably enriched in trace elements compared to those of the lower Doce River, as documented by [43], further supporting the hypothesis that terrestrial inputs from this watershed are a major driver of coastal metal enrichment at Itaoca Beach. In fact, this study only collected algae in the regions south of the mouth of the Doce River because the current carried the sediment from the mining operation to the southern region of the Atlantic Ocean located in southeastern Brazil.
The 3D Principal Component Analysis (PCA) revealed distinct patterns in metal concentrations across macroalgae samples, highlighting both species-specific and environmental influences. The first principal component (PC1), which accounts for 29.69% of the total variance, is primarily driven by vanadium (0.366), iron (0.349), chromium (0.342), and aluminum (0.315). These elements are more concentrated in Z. tournefortii, suggesting that species identity is a key determinant of metal accumulation and is reflected in the separation along PC1. The second component (PC2), explaining 15.83% of the variance, is heavily influenced by zinc (0.491), copper (0.418), nickel (0.401), and arsenic (0.404). These metals appear to capture environmental variability, likely associated with differences in pollution levels, water chemistry, and beach-specific conditions. While species remains the dominant factor, the variation along PC2 underscores the role of local environmental factors in shaping metal profiles. The third component (PC3), although accounting for a smaller portion of the variance, still provides meaningful insights. It is influenced by iron (0.380), aluminum (0.337), and mercury (0.437), suggesting more localized or species-environment interactions. The high loading of mercury particularly indicates that its accumulation may vary significantly across sites or be driven by specific conditions affecting one species more than the other. Spatial analysis of maximum metal concentrations across beaches further supports these findings. Itaoca beach exhibited the highest number of elements at peak concentrations (n = 12), followed by Imigrantes beach (n = 8), Enseada das Garças (n = 3), and Barra do Sahy beach, which showed only one element at maximum concentration. This gradient reinforces the influence of localized anthropogenic inputs and environmental conditions on trace metal accumulation in coastal macroalgae.
The detection of potentially toxic elements such as arsenic (As) and mercury (Hg) in macroalgae collected from the four studied beaches is particularly alarming due to their cumulative nature and strong bioaccumulation potential. These metals are recognized for their neurotoxic and carcinogenic effects, even at low concentrations [44]. The elevated levels of As and Hg observed in macroalgae from Itaoca beach raise serious concerns regarding the health of marine ecosystems and the safety of human populations that depend on these resources. The bioaccumulation of heavy metals in fishery products represents a significant public health risk, especially for coastal communities with high seafood consumption. Chronic exposure through contaminated food can lead to severe health outcomes, including kidney dysfunction, neurological impairments, and an increased likelihood of developing various forms of cancer [45,46].
Beyond their ecological and health implications, the accumulation of metals in macroalgae also serves as a valuable indicator of coastal water quality. Due to their capacity to absorb and retain trace elements from their surroundings, macroalgae are widely recognized as effective bioindicators for environmental monitoring [47,48]. Their use in assessing contamination levels provides critical insights into the spatial and temporal dynamics of pollution in marine environments, supporting efforts to manage and mitigate anthropogenic impacts.
Among the major groups of marine macroalgae, brown algae (Phaeophyceae) have demonstrated exceptional capacity for metal retention, positioning them as promising candidates for biotechnological applications in environmental remediation. Species belonging to the genera Sargassum and Zonaria have shown effectiveness in absorbing and retaining heavy metals, making them valuable biosorbents for the removal of contaminants from industrial effluents. According to Duart et al. [49], the biomass of these macroalgae could remove up to 96% of lead from industrial wastewater, underscoring their potential for large-scale remediation strategies. Additionally, studies such as [50] have evaluated the biosorption potential of beach-cast seaweeds, demonstrating their efficacy in extracting heavy metals from industrial solutions and liquid waste. These findings reinforce the utility of brown macroalgae not only as ecological indicators but also as functional agents in sustainable pollution control technologies.
Species of Sargassum are distinguished by their exceptional capacity to retain heavy and radioactive metals, as well as their chemical and mechanical resilience [51]. This high retention ability is largely attributed to the composition of brown macroalgal cell walls, which are rich in alginic acid—a polysaccharide containing carboxylic groups that function as cation exchangers. These groups facilitate the substitution of naturally occurring cations such as Ca2+, K+, and Na+ with heavy metal ions. This mechanism is believed to serve a protective role, trapping metals within the cell wall matrix and preventing their entry into the cytoplasm, thereby mitigating toxic effects [19].
Brown macroalgae have demonstrated an extraordinary ability to bioaccumulate metals, with studies showing that they can accumulate arsenic up to several hundred times more than terrestrial plants [52]. In some cases, the concentration of heavy metals in their cell walls can reach 20,000 to 40,000 times higher than in the surrounding water. Furthermore, Sargassum has shown promising results in the decontamination of stormwater runoff, effectively removing Pb, Cu, Zn, and Mn. Although its performance was slightly lower than that observed in synthetic metal solutions, likely due to the presence of competing ions and organic matter, the seaweed demonstrated strong potential for removing trace metals from complex environmental matrices [53].
The cell wall architecture of macroalgae varies across major taxonomic groups, with Phaeophyceae (brown), Rhodophyta (red), and many Chlorophyta (green) sharing a common structural framework composed of a fibrillar skeleton embedded in an amorphous matrix. This component is typically composed of cellulose, although substitutions occur, such as xylan replacing cellulose in Chlorophyta and Rhodophyta, while mannan can substitute cellulose in certain Chlorophyta species. In Phaeophyceae, the embedding matrix is primarily composed of alginic acid or its salt form, alginate, accompanied by smaller amounts of sulfated polysaccharides such as fucoidan. Rhodophyta, in contrast, are characterized by a matrix rich in sulfated galactans, including compounds such as agar, carrageenan, and porphyran [54]. These biochemical differences in cell wall composition not only reflect evolutionary divergence but also influence the ecological roles and biotechnological applications of each algal group.
Although statistical differences were observed in the concentrations of certain elements between Sargassum and Zonaria, these variations do not significantly alter the overall elemental distribution pattern. Interestingly, this pattern closely resembles that found in iron ore tailings, suggesting that macroalgae may exhibit a consistent elemental profile, with concentrations fluctuating in response to environmental contamination. As proposed by [29], species with similar biochemical composition, thallus morphology, and growth strategies can be effectively used for comparative assessments of elemental contamination. Particular attention should be given to elements such as arsenic (As), which is known to bioaccumulate in seaweeds—especially among brown macroalgae. This is especially relevant when considering the potential consumption of beached macroalgae by animals or humans. Fortunately, macroalgae can partially transform arsenic speciation from its more toxic inorganic form to less harmful organic compounds, thereby reducing its toxicity [37]. The data presented in this study reinforce the notion that macroalgae absorb contaminants in a pattern analogous to that observed in mining-impacted environments, further supporting their role as bioindicators of coastal pollution.

5. Conclusions

This investigation enabled the precise determination and quantification of trace-element concentrations in two marine macroalgal taxa stranded along the southeastern Brazilian coastline—a region historically impacted by inputs from iron ore tailings (IOTs), intensive mining activities, and multiple anthropogenic stressors. The congruent patterns of metal distribution observed between macroalgal tissues and IOT materials suggest that mining-derived particulates and dissolved constituents may have influenced the marine biogeochemical regime of the southeast region of Brazil, although additional tests may be required for final conclusions such as isotopic determinations. Documented environmental repercussions of mining include elevated sedimentation loads, disruption of hydrological and fluvial dynamics, and the degradation or loss of ecologically sensitive habitats. The presence of toxic elements such as arsenic (As) and mercury (Hg) in Sargassum natans and Zonaria tournefortii from the studied beaches is particularly alarming due to their cumulative nature and strong accumulation potential. These metals are known for their neurotoxic and carcinogenic effects, even at low concentrations.
Therefore, to safeguard public health and maintain the ecological integrity of coastal regions, it is imperative to implement effective control policies and invest in low-cost technologies for sediment remediation. Moreover, sustained environmental monitoring—supported by collaboration among researchers, local communities, and regulatory agencies—is essential to ensure the long-term sustainability of marine ecosystems and the food security of populations that depend on them. This integrated approach underscores the importance of ecological management in preserving coastal and oceanic waters. It also reinforces the urgency of collective action to mitigate heavy metal pollution, protect marine biodiversity, and promote the health and resilience of human communities in the face of escalating anthropogenic impacts.

Author Contributions

Conceptualization: T.H.B.; material preparation, data collection and analysis: T.H.B., B.R.R.N., A.E.N. and W.A.R.J.; formal analysis and investigation: E.S.C. and R.R.N.; writing, review and editing: T.H.B., E.S.C., R.R.N., D.H.B., D.T.B., A.T.L., I.A. and M.T.F. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the EJA (Estaleiro Jurong Aracruz—grant numbers 22/2021—FACTO/EJA/IFES) and Espírito Santo Research and Innovation Support Foundation (FAPES No. 474 10/2021).

Data Availability Statement

The original contributions presented in this study are included in the article. Further inquiries can be directed to the corresponding author.

Acknowledgments

We acknowledge Jurong Shipyard for funding the project, the Espírito Santo Research and Innovation Support Foundation (FAPES) and the Foundation for Support of Science and Technology Development (FACTO) for their support in implementing the activities. We especially thank the residents’ associations and municipal governments that supported the project. MTF thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq for the Productivity Fellowships (311195/2021-0). I.A. thanks CNPq and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Brasil (CAPES) Finance Code 001 for the PhD fellowship.

Conflicts of Interest

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Figure 1. Collection areas in southeastern Brazil, on the coast of the state of Espírito Santo. The samples were collected on the beaches of Barra do Sahy and Imigrantes (municipality of Aracruz in green) and in Enseada das Garças (Fundão in red) in the north, and on the Itaoca beach (Itapemirim in blue) in the south. The black star indicates the coastal area at the mouth of the Doce River in Espírito Santo affected by the dam collapse (note that this is an external estuary with a dispersion reaching both northward and southward currents along the coast).
Figure 1. Collection areas in southeastern Brazil, on the coast of the state of Espírito Santo. The samples were collected on the beaches of Barra do Sahy and Imigrantes (municipality of Aracruz in green) and in Enseada das Garças (Fundão in red) in the north, and on the Itaoca beach (Itapemirim in blue) in the south. The black star indicates the coastal area at the mouth of the Doce River in Espírito Santo affected by the dam collapse (note that this is an external estuary with a dispersion reaching both northward and southward currents along the coast).
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Figure 2. Trace element concentrations (mg kg−1) found in samples of the macroalgae Sargassum natans (n = 12 for beach) collected from four beaches in Espírito Santo, Brazil: Barra do Sahy (BDS), Imigrantes (IMG), Enseada das Garças (EDG) and Itaoca (ITC) in 2022. In each boxplot: the central line represents the median of the data.
Figure 2. Trace element concentrations (mg kg−1) found in samples of the macroalgae Sargassum natans (n = 12 for beach) collected from four beaches in Espírito Santo, Brazil: Barra do Sahy (BDS), Imigrantes (IMG), Enseada das Garças (EDG) and Itaoca (ITC) in 2022. In each boxplot: the central line represents the median of the data.
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Figure 3. Trace element concentrations (mg kg−1) found in samples of the macroalgae Zonaria tournefortii (n = 12 for beach) collected from four beaches in Espírito Santo, Brazil: Barra do Sahy (BDS), Imigrantes (IMG), Enseada das Garças (EDG) and Itaoca (ITC) in 2022. In each boxplot: the central line represents the median of the data.
Figure 3. Trace element concentrations (mg kg−1) found in samples of the macroalgae Zonaria tournefortii (n = 12 for beach) collected from four beaches in Espírito Santo, Brazil: Barra do Sahy (BDS), Imigrantes (IMG), Enseada das Garças (EDG) and Itaoca (ITC) in 2022. In each boxplot: the central line represents the median of the data.
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Figure 4. 3D Principal Component Analysis (PCA) plot showing the distribution of metal trace concentrations in two macroalgae species, Sargassum natans (red) and Zonaria tournefortii (blue), collected from four different beaches at the Espirito Santo coast, Brazil, in 2022.
Figure 4. 3D Principal Component Analysis (PCA) plot showing the distribution of metal trace concentrations in two macroalgae species, Sargassum natans (red) and Zonaria tournefortii (blue), collected from four different beaches at the Espirito Santo coast, Brazil, in 2022.
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Table 1. Characteristics of the beaches and geographic coordinates of macroalgae collection areas in Espírito Santo, southeastern Brazil.
Table 1. Characteristics of the beaches and geographic coordinates of macroalgae collection areas in Espírito Santo, southeastern Brazil.
MunicipalityBeach/
Geographic
Coordinates		Characteristics of the Beaches
Aracruz
(94,765 inhabitants)		Barra do Sahy

19°52′28.056″ S
40°4′44.328″ W
  • Urbanized and tourist spot with beachfront restaurants.
  • Occurrence of small (<2 m height) sandbank vegetation.
  • Near the Jurong Shipyard, Portocel, and Suzano pulp mill and 50 km from the Doce River mouth.
  • State near the mouth of the Doce River.
Imigrantes

19°57′13.9″ S
40°08′29.3″ W
  • Tourist spot, with a significant presence of sandbank vegetation (>2 m height).
  • Part of two Federal Conservation Units (Costa das Algas Environmental Protection Area and Santa Cruz Wildlife Refuge) along the banks of the Piraqueaçu River estuary.
  • State near the mouth of the Doce River.
Fundão
(21,948 inhabitants)		Enseada das

Garças
20°1′49.908″ S
40°9′31.860″ W
  • Tourist spot, located in a Federal Conservation Unit (Costa das Algas).
  • Unurbanized beach with large (>2 m height) sandbank vegetation on the beach.
  • State near the mouth of the Doce River.
Itapemirim
(34,656 inhabitants)		Itaoca

20°54′23.220″ S
40°46′49.908″ W
  • Tourist beach with intense urbanization. Close to Itaipava fishing port. Near the mouth of the Itapemirim River.
  • Fishing vessel traffic in the area. Beachfront restaurants and a small (<2 m height) area of sandbank vegetation.
Source: [22].
Table 2. Limits of detection (LOD) and quantification (LOQ) of the elements analyzed in this study in parts per billion (ppb).
Table 2. Limits of detection (LOD) and quantification (LOQ) of the elements analyzed in this study in parts per billion (ppb).
Elementsppb
LODLOQ
Ag0.0300.090
Al0.912.76
As0.0480.147
Ba0.0450.136
Cd0.0020.007
Co0.0060.019
Cr0.0210.064
Cu0.0540.162
Fe2.557.72
Hg0.0360.110
Mn0.1010.306
Ni0.0740.224
Pb0.0210.062
V0.0070.020
Zn0.0890.269
Table 3. Comparison of 15 elements between beach-cast seaweeds (Zonaria tournefortii and Sargassum natans) collected at four beaches in Espírito Santo (Brazil) and submerged samples of Sargassum natans (as S. vulgare) from the Syrian Sea [28], Ceará (Brazil) [26], and Madeira Archipelago (Portugal) [27]. The concentrations are presented as median ± interquartile range (IQR) or mean ± standard deviation (SD), depending on the study.
Table 3. Comparison of 15 elements between beach-cast seaweeds (Zonaria tournefortii and Sargassum natans) collected at four beaches in Espírito Santo (Brazil) and submerged samples of Sargassum natans (as S. vulgare) from the Syrian Sea [28], Ceará (Brazil) [26], and Madeira Archipelago (Portugal) [27]. The concentrations are presented as median ± interquartile range (IQR) or mean ± standard deviation (SD), depending on the study.
Samples AnalyzedBeach-CastSubmerged
SpeciesZonaria
tournefortii		Sargassum
natans		Sargassum natans (as S. vulgare)
Sampling SitesEspírito SantoEspírito SantoSyrian SeaCeará
(Dry Season)		Ceará
(Rainy Season)		Madeira Archipelago
Metal (mg kg−1)Median ± IQRMedian ± IQRMean ± SDMean ± SDMean ± SDMean ± SD
Aluminum (Al)2255 ± 9601612 ± 10802898 ± 119174 ± 0.12199 ± 130-
Iron (Fe)2184 ± 12081352 ± 8337382 ± 20299 ± 71730 ± 117-
Manganese (Mn)74.9 ± 41.278.6 ± 64.1129 ± 58.48 ± 0.51220.8 ± 8.4-
Barium (Ba)29.4 ± 7.931.4 ± 9.523 ± 11.10 ± 0.0527.9 ± 0.3-
Arsenic (As)19.2 ± 8.818.4 ± 10.593.2 ± 3.15.0 ± 0.3172 ± 6-
Zinc (Zn)17 ± 8.217.1 ± 6.423.74 ± 1.19<LOD17.6 ± 1.825.20 ± 4.97
Vanadium (V)8.80 ± 3.85.70 ± 4.611.07 ± 7.38<LOD<LOD-
Copper (Cu)3.70 ± 2.53.20 ± 1.74.75 ± 0.18<LOD2.36 ± 0.171.37 ± 0.04
Chromium (Cr)3.50 ± 1.42.30 ± 1.923.1 ± 1.00.130 ± 0.0076.77 ± 0.481.19 ± 0.3
Nickel (Ni)3.20 ± 2.53.00 ± 2.40.71 ± 0.1<LOD<LOD1.37 ± 0.08
Lead (Pb)1.60 ± 1.11.10 ± 1.21.04 ± 0.05<LOD<LOD0.40 ± 0.04
Cobalt (Co)0.50 ± 0.30.60 ± 0.33.60 ± 0.330.130 ± 0.012.16 ± 0.06-
Cadmium (Cd)0.40 ± 0.20.40 ± 0.20.16 ± 0.030.050 ± 0.0041.41 ± 0.011.75 ± 0.07
Mercury (Hg)0.20 ± 0.10.10 ± 0.20.06 ± 0.010.634 ± 0.0581.479 ± 0.050.04 ± 0.01
Silver (Ag)0.10 ± 0.10.10 ± 0.1----
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MDPI and ACS Style

Basilio, T.H.; Nunes, B.R.R.; Neto, A.E.; Bonemann, D.H.; Bueno, D.T.; Fujii, M.T.; Alonso, I.; Lima, A.T.; Rodrigues Junior, W.A.; Costa, E.S.; et al. Trace Metal Concentration in Beach-Cast Seaweeds from Southeastern Brazil Indicates the Legacy of the Mining Industry. Phycology 2026, 6, 44. https://doi.org/10.3390/phycology6020044

AMA Style

Basilio TH, Nunes BRR, Neto AE, Bonemann DH, Bueno DT, Fujii MT, Alonso I, Lima AT, Rodrigues Junior WA, Costa ES, et al. Trace Metal Concentration in Beach-Cast Seaweeds from Southeastern Brazil Indicates the Legacy of the Mining Industry. Phycology. 2026; 6(2):44. https://doi.org/10.3390/phycology6020044

Chicago/Turabian Style

Basilio, Thiago Holanda, Bianca Rodrigues Ramalhete Nunes, Angélica Elaine Neto, Daisa Hakbart Bonemann, Danielle Tapia Bueno, Mutue T. Fujii, Iago Alonso, Ana Teresa Lima, Weber Adão Rodrigues Junior, Eduardo Schiettini Costa, and et al. 2026. "Trace Metal Concentration in Beach-Cast Seaweeds from Southeastern Brazil Indicates the Legacy of the Mining Industry" Phycology 6, no. 2: 44. https://doi.org/10.3390/phycology6020044

APA Style

Basilio, T. H., Nunes, B. R. R., Neto, A. E., Bonemann, D. H., Bueno, D. T., Fujii, M. T., Alonso, I., Lima, A. T., Rodrigues Junior, W. A., Costa, E. S., & Rodrigues Neto, R. (2026). Trace Metal Concentration in Beach-Cast Seaweeds from Southeastern Brazil Indicates the Legacy of the Mining Industry. Phycology, 6(2), 44. https://doi.org/10.3390/phycology6020044

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