Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microfluidic Component Printing and Assembly
2.2. Cell Culture, Fluorescence Labeling, and Live–Dead Staining
2.3. Bacterial Culture
2.4. Fluorescence Microscopy
2.5. Mixing and Separation Particles of Cells with Bacteria
3. Results
3.1. Microfluidic Channels
3.2. Mixer
3.3. Dean Flow Helix
3.3.1. Establishment of Microfluidic Components
3.3.2. Application Examples: Separating Small and Large Particles in Dean’s Flow Helix
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Flow rate in µL/min | 50 | 100 | 200 |
Flow velocity in m/s | 0.017 | 0.034 | 0.068 |
Reynolds number | 4.2 | 8.5 | 16.9 |
Deans number (Equation (1)) | 0.39 | 0.77 | 1.54 |
Particle Reynolds number (Ø 1.95 µm) | 0.0003 | 0.0005 | 0.0010 |
Particle Reynolds number (Ø 11.95 µm) | 0.0096 | 0.0193 | 0.0386 |
Focusing of large particles achieved | No | No | Yes |
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Wiede, A.; Stranik, O.; Tannert, A.; Neugebauer, U. Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation. Micro 2023, 3, 671-685. https://doi.org/10.3390/micro3030047
Wiede A, Stranik O, Tannert A, Neugebauer U. Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation. Micro. 2023; 3(3):671-685. https://doi.org/10.3390/micro3030047
Chicago/Turabian StyleWiede, Alexander, Ondrej Stranik, Astrid Tannert, and Ute Neugebauer. 2023. "Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation" Micro 3, no. 3: 671-685. https://doi.org/10.3390/micro3030047