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Article

Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems

by
Vladimir Binyukov
,
Elena Mil
,
Ludmila Matienko
*,
Anastasia Albantova
and
Alexander Goloshchapov
Emanuel Institute of Biochemical Physics Russian Academy of Science, 4 Kosygin Street, 119334 Moscow, Russia
*
Author to whom correspondence should be addressed.
Micro 2023, 3(2), 382-390; https://doi.org/10.3390/micro3020026
Submission received: 8 February 2023 / Revised: 28 February 2023 / Accepted: 23 March 2023 / Published: 28 March 2023
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Abstract

For the first time AFM (atomic-force microscopy) was used to record significant changes in the geometric parameters of the image of erythrocytes in vitro under conditions of glycolytic starvation (ATP (Adenosine triphosphate) deficiency). The difference in the action of antioxidants, phenosan K, and Ihfan-10 on erythrocytes that we detected with AFM seems to be mainly due to their difference in hydrophobicity. We used the AFM method to research the self-organization of the components of the active center of P450 (Porphyrin-450) metalloenzymes that are part of a class of hemoproteins with functions of affinity to molecular oxygen O2. Stable supramolecular nanostructures in the form of triangular prisms based on the iron porphyrin complex with amino acids due to self-assembly involving intermolecular hydrogen bonds were received. A possible scheme for the formation of such structures is proposed.
Keywords: AFM; erythrocytes; glucose; ATP deficiency; phenolic antioxidants; nanostructures; H-bonds; Hemin; L-Histidine; L-Tyrosine AFM; erythrocytes; glucose; ATP deficiency; phenolic antioxidants; nanostructures; H-bonds; Hemin; L-Histidine; L-Tyrosine

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MDPI and ACS Style

Binyukov, V.; Mil, E.; Matienko, L.; Albantova, A.; Goloshchapov, A. Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems. Micro 2023, 3, 382-390. https://doi.org/10.3390/micro3020026

AMA Style

Binyukov V, Mil E, Matienko L, Albantova A, Goloshchapov A. Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems. Micro. 2023; 3(2):382-390. https://doi.org/10.3390/micro3020026

Chicago/Turabian Style

Binyukov, Vladimir, Elena Mil, Ludmila Matienko, Anastasia Albantova, and Alexander Goloshchapov. 2023. "Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems" Micro 3, no. 2: 382-390. https://doi.org/10.3390/micro3020026

APA Style

Binyukov, V., Mil, E., Matienko, L., Albantova, A., & Goloshchapov, A. (2023). Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems. Micro, 3(2), 382-390. https://doi.org/10.3390/micro3020026

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