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Cerium Oxide Nanoparticles: Synthesis and Characterization for Biosafe Applications

1
Center for Biotechnology and Biomedical Sciences, Molecular Toxicology Laboratory, Norfolk State University, 700 Park Avenue, Norfolk, VA 23504, USA
2
Center for Materials Research, Norfolk State University, 555 Park Avenue, Norfolk, VA 23504, USA
3
Department of Bioinformatics and Biotechnology, Jnanashakthi Campus, Akkamahadevi Women’s University, Vijayapura 586108, Karnataka, India
*
Author to whom correspondence should be addressed.
Academic Editor: Ana María Díez-Pascual
Nanomanufacturing 2021, 1(3), 176-189; https://doi.org/10.3390/nanomanufacturing1030013
Received: 25 August 2021 / Revised: 25 November 2021 / Accepted: 29 November 2021 / Published: 3 December 2021
Due to its excellent physicochemical properties, cerium oxide (CeO2) has attracted much attention in recent years. CeO2 nanomaterials (nanoceria) are widely being used, which has resulted in them getting released to the environment, and exposure to humans (mostly via inhalation) is a major concern. In the present study, CeO2 nanoparticles were synthesized by hydroxide-mediated method and were further characterized by Scanning Electron Microscopy (SEM), Field Emission Scanning Electron Microscopy (FESEM), Energy Dispersive X-ray Spectroscopy (EDX), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Diffraction Spectroscopy (XRD). Human lung epithelial (Beas-2B) cells were used to assess the cytotoxicity and biocompatibility activity of CeO2 nanoparticles. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and Live/Dead assays were performed to determine the cytotoxicity and biocompatibility of CeO2 nanoparticles. Generation of reactive oxygen species (ROS) by cerium oxide nanoparticles was assessed by ROS assay. MTT assay and Live/Dead assays showed no significant induction of cell death even at higher concentrations (100 μg per 100 μL) upon exposure to Beas-2B cells. ROS assay revealed that CeO2 nanoparticles did not induce ROS that contribute to the oxidative stress and inflammation leading to various disease conditions. Thus, CeO2 nanoparticles could be used in various applications including biosensors, cancer therapy, catalytic converters, sunscreen, and drug delivery. View Full-Text
Keywords: cerium oxide nanoparticles; hydroxide-mediated method; microscopy; spectroscopy; cytotoxicity; biocompatibility; reactive oxygen species cerium oxide nanoparticles; hydroxide-mediated method; microscopy; spectroscopy; cytotoxicity; biocompatibility; reactive oxygen species
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MDPI and ACS Style

Tumkur, P.P.; Gunasekaran, N.K.; Lamani, B.R.; Nazario Bayon, N.; Prabhakaran, K.; Hall, J.C.; Ramesh, G.T. Cerium Oxide Nanoparticles: Synthesis and Characterization for Biosafe Applications. Nanomanufacturing 2021, 1, 176-189. https://doi.org/10.3390/nanomanufacturing1030013

AMA Style

Tumkur PP, Gunasekaran NK, Lamani BR, Nazario Bayon N, Prabhakaran K, Hall JC, Ramesh GT. Cerium Oxide Nanoparticles: Synthesis and Characterization for Biosafe Applications. Nanomanufacturing. 2021; 1(3):176-189. https://doi.org/10.3390/nanomanufacturing1030013

Chicago/Turabian Style

Tumkur, Prathima P., Nithin K. Gunasekaran, Babu R. Lamani, Nicole Nazario Bayon, Krishnan Prabhakaran, Joseph C. Hall, and Govindarajan T. Ramesh. 2021. "Cerium Oxide Nanoparticles: Synthesis and Characterization for Biosafe Applications" Nanomanufacturing 1, no. 3: 176-189. https://doi.org/10.3390/nanomanufacturing1030013

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