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A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA

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National Wildlife Health Center, U.S. Geological Survey, Madison, WI 53711, USA
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La Crosse Fish Health Center-Midwest Fisheries Center, U.S. Fish and Wildlife Service, Onalaska, WI 54650, USA
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Department of Pathobiological Sciences and Freshwater & Marine Sciences Program, University of Wisconsin-Madison, Madison, WI 53711, USA
4
Southwestern Virginia Field Office, U.S. Fish and Wildlife Service, Abingdon, VA 24210, USA
5
Upper Midwest Environmental Sciences Center, U.S. Geological Survey, La Crosse, WI 54603, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Edit Eszterbauer
Parasitologia 2022, 2(1), 1-12; https://doi.org/10.3390/parasitologia2010001
Received: 27 October 2021 / Revised: 6 December 2021 / Accepted: 15 December 2021 / Published: 1 January 2022
Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of their population decline are often enigmatic, with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event in the Clinch River, Virginia and Tennessee, USA. Histopathology and transmission electron microscopy showed a novel microsporidian parasite primarily infecting the ovary of pheasantshell. Sequencing of the small subunit rRNA gene produced a 1333 bp sequence with the greatest similarity to Pseudonosema cristatellae (AF484694.1; 86.36%; e-value = 0), a microsporidium infecting the freshwater bryozoan (Cristatella mucedo). Microsporidia were observed in 65% (17/26) of the examined female pheasantshell (A. pectorosa) and in no (0/2) female muckets (A. ligamentina) and occurred at mortality and non-mortality sites. Our findings indicate that a novel parasite, Microsporidium clinchi n. sp., is present in pheasantshell in the Clinch River, and while likely not a cause of mass mortality, could reduce fecundity and recruitment in this declining population and threaten the success of reintroductions. Surveillance of M. clinchi n. sp. and evaluation of broodstock and their progeny for microsporidia would therefore be prudent. View Full-Text
Keywords: Actinonaias pectorosa; bivalve; freshwater mussel; histopathology; microsporidia; Microsporidium clinchi n. sp.; PCR; TEM; unionid Actinonaias pectorosa; bivalve; freshwater mussel; histopathology; microsporidia; Microsporidium clinchi n. sp.; PCR; TEM; unionid
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MDPI and ACS Style

Knowles, S.; Leis, E.M.; Richard, J.C.; Cole, R.; Agbalog, R.E.; Putnam, J.G.; Goldberg, T.L.; Waller, D.L. A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA. Parasitologia 2022, 2, 1-12. https://doi.org/10.3390/parasitologia2010001

AMA Style

Knowles S, Leis EM, Richard JC, Cole R, Agbalog RE, Putnam JG, Goldberg TL, Waller DL. A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA. Parasitologia. 2022; 2(1):1-12. https://doi.org/10.3390/parasitologia2010001

Chicago/Turabian Style

Knowles, Susan, Eric M. Leis, Jordan C. Richard, Rebecca Cole, Rose E. Agbalog, Joel G. Putnam, Tony L. Goldberg, and Diane L. Waller. 2022. "A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA" Parasitologia 2, no. 1: 1-12. https://doi.org/10.3390/parasitologia2010001

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