Antioxidant Capacity of Some Extracts from Aronia and Lonicera Fruits †
by
, and
Valentina Cristina Negru
1, 
Eliza Oprea
1,
Claudia Nicola
2,
Maria Marinescu
1 and
Claudia-Valentina Popa
1,3,* 1
Faculty of Chemistry, University of Bucharest, 90-92 Panduri Street, 050657 Bucharest, Romania
2
Research Institute for Fruit Growing, 402 Marului Street, Pitesti-Maracineni, 117450 Arges County, Romania
3
Cantacuzino National Military Medical Institute for Research and Development, 103 Splaiul Independenței, 050096 Bucharest, Romania
*
Author to whom correspondence should be addressed.
†
Presented at the 17th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 27–29 October 2021.
Chem. Proc. 2022, 7(1), 83; https://doi.org/10.3390/chemproc2022007083
Published: 30 June 2022
(This article belongs to the Proceedings of The 17th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM)
Studies in the literature have shown high levels of phenolic compounds in the fruits of Aronia melanocarpa (fam. Rosaceae, black chokeberry) and Lonicera caerulea L. (fam. Caprifoliaceae, Siberian blueberry). Aronia fruits contain high levels of flavonoids and have significant antioxidant activity [1]. It has been observed that Lonicera fruits have many beneficial effects: anti-aging, anti-inflammatory, antimicrobial, anticancer, cardio- and neuroprotective activity, etc. [2]. In addition, it is known that all of these pathologies are strongly associated with free radical formation and lipid peroxidation, also involved in many other diseases [1]. This study assessed the antioxidant capacity of ethanolic extracts from chokeberries and blueberries obtained by Soxhlet extraction, maceration, ultrasounds, and microwave-assisted extraction methods. First, the phenol compounds from extracts were analyzed by HPLC [3]. Then, the antioxidant capacity was determined by the DPPH● method, and the total phenol (TP) content was investigated using the Folin–Ciocalteu reagent (spectrophotometric methods) [4]. The range of quantified extraction yields varied from 10.8 to 15.2 g semi-solid extract/100 g fruit. P-coumaric acid, caffeic acid, chlorogenic acid, rutin, and epicatechin were identified in the chromatographic method in all extracts. The scavenging capacity of DPPH● was expressed as µg antioxidant/mL equivalents and varied between 2.07 ± 0.066 and 4.01 ± 0.069 for gallic acid, 4.25 ± 0.13 and 8.15 ± 0.14 for caffeic acid, 23.4 ± 0.63 and 41.6 ± 0.65 for quercetin, 22 ± 0.52 and 37.0 ± 0.54 rutin, and 37.2 ± 1.1 and 68.3 ± 1.1 for morin. Ultrasound-assisted extraction was the most efficient method of extraction. The berry extracts also had a high antioxidant capacity, expressed in terms of flavonoid equivalents. The ultrasound extraction was the most efficient method of extraction. The present study concludes that Aronia and Lonicera fruits show high antioxidant content, as proven by chromatographic and spectrophotometric methods.
Funding
This research received no external funding.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Not applicable.
Conflicts of Interest
The authors declare no conflict of interest.
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MDPI and ACS Style
Negru, V.C.; Oprea, E.; Nicola, C.; Marinescu, M.; Popa, C.-V. Antioxidant Capacity of Some Extracts from Aronia and Lonicera Fruits. Chem. Proc. 2022, 7, 83. https://doi.org/10.3390/chemproc2022007083
AMA Style
Negru VC, Oprea E, Nicola C, Marinescu M, Popa C-V. Antioxidant Capacity of Some Extracts from Aronia and Lonicera Fruits. Chemistry Proceedings. 2022; 7(1):83. https://doi.org/10.3390/chemproc2022007083
Chicago/Turabian StyleNegru, Valentina Cristina, Eliza Oprea, Claudia Nicola, Maria Marinescu, and Claudia-Valentina Popa. 2022. "Antioxidant Capacity of Some Extracts from Aronia and Lonicera Fruits" Chemistry Proceedings 7, no. 1: 83. https://doi.org/10.3390/chemproc2022007083
