The Effectiveness of Suffruticosol B in Treating Lung Cancer by the Laser Trapping Technique

Round 1

Reviewer 1 Report

The Effectiveness of Suffruticosol B in Treating Lung Cancer by Laser Trapping Technique

Abstract section:

Authors must cite numerical data to support their abstract.

Introduction section.

Eliminate number 3 (subindex)

See line 33

“these3 [3]”.

See line 38

Replace the form of cites [4-8 3-7] by [3-8].

See lines 69 and 70.

Replace Paeonia suffruticosa by Paeonia suffruticosa (in italic form)

Methods section.

The authors must mention where the Suffruticosol B was obtained from, if it was of natural or synthetic origin and write the corresponding reference.

See lines 143 and 144.

“The cells’ Images from the three samples are presented in Fig. 1.”

The cell´s images are presented in Figure 2, but not in figure 1. Please correct this.

Discussion section.

See line 278.

Replace Sufffruticosol by Suffruticosol B.

See line 301 “The program Origin 6.2.” Please write the appropriate reference for the software used.

See line 307. “ in Fig. 8”. Figure 8 is missing please correct this.

Author Response

We would like to thank reviewer the time invested in reviewing this paper and the suggestions that was made.

Reviewer 1

Abstract section:

Authors must cite numerical data to support their abstract.

Response 

We have rewrite the abstract to accommodate the reviewer suggestion.

Reviewer 1

Eliminate number 3 (subindex)

See line 33.     “these[3]”.

Response

 This was fixed. See line 47

Reviewer 1

See line 38.      

Replace the form of cites [4-8 3-7] by [3-8].    

Response

 This was fixed. See line 52

Reviewer 1

See lines 69 and 70.             

Replace Paeonia suffruticosa by Paeonia suffruticosa (in italicform).   

Response

 This was fixed. See lines 89-91

Reviewer 1

Methods section.

The authors must mention where the Suffruticosol B was obtained from, if it was of natural or synthetic origin and write the corresponding reference.

Response

 This was fixed. We added  lines 113-121 to address this concern.

Reviewer 1

See lines 143 and 144.     

“The cells’ Images from the three samples are presented in Fig. 1.”

The cell´s images are presented in Figure 2, but not in figure 1.Please correct this.

Response

 This was fixed. See line 257

Reviewer 1

Discussion section.

See line 278.

Replace Sufffruticosol by Suffruticosol B. 

Response

 This was fixed. See line 397

Reviewer 1

See line 301 “The program Origin 6.2.” Please write the appropriate reference for the software used.  

Response

 This was fixed. See line 437

REVIEWER 1

See line 307. “ in Fig. 8”. Figure 8 is missing please correct this.   

Response

Fixed. This should have been Fig. 7. See line 451

Reviewer 2 Report

In the present paper, the suthors studied the the simultaneous effect of the laser trapping and the Suffruticosol B on the cancel cells A549. Authors varied the time of the treatment and studied the size effect isung the laser trapping. Despite a rather poor set of methods was used, the work is rather unusual and may be of interest for the readership of Biophysica.

The reviewer would suggests to extend the number of the cell lines to make the paper more rigid; otherwise it looks more like a communication.

Otherwise, please change the order of pictures in Figures: a) should be on the top and c) - in the bottom.

Besides, the conclusion must conclude the work. The statement "This cell line responded interestingly to 354 the treatment" should be clarified, and short, but clear explanation of "interestingly" must be given.

Author Response

We would like to thank the reviewer for the time taken to review this manuscript. We have done our due diligence to address the reviewers' concern. 

Reviewer 2

The reviewer would suggests to extend the number of the cell lines to make the paper more rigid; otherwise it looks more like a communication.

Response

We agree that more cell lines would make the paper more rigid.  We believe that data collected is still interesting and make for a good publication. Further, number of publish articles have look at physical properties on single cell.

Reviewer 2

Otherwise, please change the order of pictures in Figures: a) should be on the top and c) - in the bottom.

Response

This was fixed 

Reviewer 2

Besides, the conclusion must conclude the work. The statement "This cell line responded interestingly to 354 the treatment" should be clarified, and short, but clear explanation of "interestingly" must be given.

Response

I believe we address this concern  please see  lines 467-495, and 531-535

Round 2

Reviewer 2 Report

The paper can be accepted now.