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Article

A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker α-Amylase in Point-of-Care Applications

CIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, Portugal
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Academic Editor: Masato Sone
Electrochem 2021, 2(3), 427-438; https://doi.org/10.3390/electrochem2030028
Received: 15 June 2021 / Revised: 16 July 2021 / Accepted: 21 July 2021 / Published: 26 July 2021
(This article belongs to the Collection Feature Papers in Electrochemistry)
The design and synthesis of artificial receptors based on molecular imprinting (MI) technology for the development of a new MIP-based biosensor for detection of the stress biomarker α-amylase in human saliva in point-of-care (PoC) applications is described in this work. The portable electrochemical devices for monitoring α-amylase consists of cost-effective and disposable gold screen-printed electrodes (AuSPEs). To build the electrochemical device, the template biomolecule was firstly immobilized directly over the working area of the gold chip previously activated with a self-assembled monolayer (SAM) of cysteamine (CA). Then, pyrrole (Py) monomer was selected as building block of a polymeric network prepared by CV electropolymerization. After the electropolymerization process, the enzyme was removed from the polymer film in order to build the specific recognition sites for the target enzyme. The MIP biosensor showed a very wide linear concentration range (between 3.0 × 10−4 to 0.60 mg mL−1 in buffer solution and between 3.0 × 10−4 to 3.0 × 10−2 mg mL−1 in human saliva) and low detection levels were achieved (LOD < 3.0 × 10−4 mg mL−1) using square wave voltammetry (SWV) as the electroanalytical technique. View Full-Text
Keywords: α-amylase; pyrrole (Py); electrochemical biosensor; molecularly imprinted polymer (MIP); screen-printed electrode (SPE) α-amylase; pyrrole (Py); electrochemical biosensor; molecularly imprinted polymer (MIP); screen-printed electrode (SPE)
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MDPI and ACS Style

Rebelo, T.S.C.R.; Miranda, I.M.; Brandão, A.T.S.C.; Sousa, L.I.G.; Ribeiro, J.A.; Silva, A.F.; Pereira, C.M. A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker α-Amylase in Point-of-Care Applications. Electrochem 2021, 2, 427-438. https://doi.org/10.3390/electrochem2030028

AMA Style

Rebelo TSCR, Miranda IM, Brandão ATSC, Sousa LIG, Ribeiro JA, Silva AF, Pereira CM. A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker α-Amylase in Point-of-Care Applications. Electrochem. 2021; 2(3):427-438. https://doi.org/10.3390/electrochem2030028

Chicago/Turabian Style

Rebelo, Tânia S.C.R., Inês M. Miranda, Ana T.S.C. Brandão, Laura I.G. Sousa, José A. Ribeiro, António F. Silva, and Carlos M. Pereira. 2021. "A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker α-Amylase in Point-of-Care Applications" Electrochem 2, no. 3: 427-438. https://doi.org/10.3390/electrochem2030028

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