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Special Issue on Plasma Medicine
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Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness

1
Department of Health Sciences, University of Piemonte Orientale, 28100 Novara, Italy
2
Department of Industrial Engineering, Alma Mater Studiorum-Università di Bologna, 40100 Bologna, Italy
3
Interdepartmental Centre for Industrial Research, Advanced Applications in Mechanical Engineering and Materials Technology, Alma Mater Studiorum-Università di Bologna, 40100 Bologna, Italy
4
R & D Biolab, 28100 Novara, Italy
5
Interdepartmental Centre for Industrial Research Agrifood, Alma Mater Studiorum-Università di Bologna, 47521 Cesena, Italy
*
Authors to whom correspondence should be addressed.
The authors have contributed equally.
Current address: GREMI, UMR7344 CNRS/Université d’Orléans, 45067, Orléans, France.
Plasma 2018, 1(2), 261-276; https://doi.org/10.3390/plasma1020023
Received: 30 September 2018 / Revised: 19 October 2018 / Accepted: 19 October 2018 / Published: 29 October 2018
(This article belongs to the Special Issue Plasma Medicine)
Monocytes are involved in innate immune surveillance, establishment and resolution on inflammation, and can polarize versus M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages. The possibility to control and drive immune cells activity through plasma stimulation is therefore attractive. We focused on the effects induced by cold-atmospheric plasma on human primary monocytes and monocyte-derived macrophages. Monocytes resulted more susceptible than monocyte-derived macrophages to the plasma treatment as demonstrated by the increase in reactive oxygen (ROS) production and reduction of viability. Macrophages instead were not induced to produce ROS and presented a stable viability. Analysis of macrophage markers demonstrated a time-dependent decrease of the M1 population and a correspondent increase of M2 monocyte-derived macrophages (MDM). These findings suggest that plasma treatment may drive macrophage polarization towards an anti-inflammatory phenotype. View Full-Text
Keywords: cold atmospheric pressure plasma; dielectric barrier discharges; monocytes; monocytes-derived macrophages cold atmospheric pressure plasma; dielectric barrier discharges; monocytes; monocytes-derived macrophages
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Crestale, L.; Laurita, R.; Liguori, A.; Stancampiano, A.; Talmon, M.; Bisag, A.; Gherardi, M.; Amoruso, A.; Colombo, V.; Fresu, L.G. Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness. Plasma 2018, 1, 261-276.

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