Plasma Treatment of Ovarian Cancer Cells Mitigates Their Immuno-Modulatory Products Active on THP-1 Monocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture, Plasma Treatment, and Supernatants
2.2. Metabolic Activity and Viability of Ovarian Cancer Cells
2.3. High Content Imaging of THP-1 Monocytes
2.4. Metabolic Activity and Flow Cytometry of THP-1 Monocytes
2.5. Supernatant Analysis
2.6. Statistical Analysis
3. Results
3.1. Plasma Treatment Abrogated Growth of OVCAR-3 and SKOV-3 Cells
3.2. Supernatants of Plasma-Treated Ovarian Cancer Cell Lines Modestly Affected THP-1 Monocytes’ Oxidative Balance and Metabolic Activity
3.3. Plasma Treatment of Ovarian Cancer Cell Lines Alleviated Effects of Their Supernatants on THP-1 Monocyte Motility and Morphology
3.4. Plasma Treatment of SKOV-3 Cells Mitigated Effects of Their Supernatants on THP-1 Monocyte Surface Marker Expression
3.5. Plasma Treatment of Ovarian Cancer Cells Changed Their Secretory Products and Those of THP-1 Cells
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Source | THP-1 Monocyte Supernatants | OVCAR-3 Supernatants | SKOV-3 Supernatants | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Target | Statistic | Vehicle | Ov-Ctrl | Ov-Pl | Sk-Ctrl | Sk-Pl | Ctrl | Pl | Ctrl | Pl |
IL1β | Mean | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 |
S.D. | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
IL8 | Mean | 2.1 | 40.2 | 2.7 *** | 18.6 | 3.6 | 133.3 | 280.1 | 33.8 | 69.1 ** |
S.D. | 0.0 | 0.9 | 0.8 | 28.6 | 2.6 | 59.4 | 99.1 | 6.8 | 9.6 | |
IL10 | Mean | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 |
S.D. | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
IL12p70 | Mean | 1.3 | 1.3 | 1.3 | 1.3 | 1.3 | 1.3 | 1.3 | 1.3 | 1.3 |
S.D. | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
IL17 | Mean | 4.2 | 4.2 | 4.2 | 4.2 | 4.2 | 4.2 | 4.2 | 4.2 | 4.2 |
S.D. | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
IL18 | Mean | 3.5 | 7.9 | 2.7 ** | 2.0 | 2.2 | 7.1 | 29.5 * | 1.3 | 1.3 |
S.D. | 1.4 | 0.4 | 0.9 | 1.2 | 0.8 | 3.0 | 8.4 | 0.0 | 0.0 | |
IL23 | Mean | 3.4 | 3.4 | 3.4 | 3.4 | 3.4 | 3.4 | 3.4 | 3.4 | 3.4 |
S.D. | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
IL33 | Mean | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 |
S.D. | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
IFNα | Mean | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 |
S.D. | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
IFNγ | Mean | 2.7 | 2.7 | 2.7 | 2.7 | 2.7 | 2.7 | 2.7 | 2.7 | 2.7 |
S.D. | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
MCP1 | Mean | 12.2 | 14.1 | 17.3 | 44.5 | 13.6 *** | 3.1 | 3.1 | 3.1 | 3.1 |
S.D. | 2.7 | 3.2 | 3.1 | 2.3 | 2.3 | 0.0 | 0.0 | 0.0 | 0.0 | |
TNFα | Mean | 1.7 | 1.7 | 1.7 | 1.7 | 1.7 | 1.7 | 4.7 ** | 1.7 | 1.7 |
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Bekeschus, S.; Wulf, C.P.; Freund, E.; Koensgen, D.; Mustea, A.; Weltmann, K.-D.; Stope, M.B. Plasma Treatment of Ovarian Cancer Cells Mitigates Their Immuno-Modulatory Products Active on THP-1 Monocytes. Plasma 2018, 1, 201-217. https://doi.org/10.3390/plasma1010018
Bekeschus S, Wulf CP, Freund E, Koensgen D, Mustea A, Weltmann K-D, Stope MB. Plasma Treatment of Ovarian Cancer Cells Mitigates Their Immuno-Modulatory Products Active on THP-1 Monocytes. Plasma. 2018; 1(1):201-217. https://doi.org/10.3390/plasma1010018
Chicago/Turabian StyleBekeschus, Sander, Can Pascal Wulf, Eric Freund, Dominique Koensgen, Alexander Mustea, Klaus-Dieter Weltmann, and Matthias B. Stope. 2018. "Plasma Treatment of Ovarian Cancer Cells Mitigates Their Immuno-Modulatory Products Active on THP-1 Monocytes" Plasma 1, no. 1: 201-217. https://doi.org/10.3390/plasma1010018