The Effect of Tocopherol-α on the Cell Viability in Caco-2 Cell Line †
Department of Biochemistry, Faculty of Veterinary Medicine, Ankara University, Yenimahalle 06560,Turkey
*
Author to whom correspondence should be addressed.
†
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
Proceedings 2017, 1(10), 1062; https://doi.org/10.3390/proceedings1101062
Published: 16 November 2017
(This article belongs to the Proceedings of Proceedings of the 2nd International Conference on Natural Products for Cancer Prevention and Therapy)
Abstract
:Tocopherols, the major forms of vitamin E, are a family of fat-soluble phenolic compounds. Each tocopherol contains a chromanol ring system and a phytyl chain containing 16 carbons. Tocopherols, as effective antioxidants, have been proposed to protect against carcinogenesis. Colon carsinoma cell line were treated with tocopherol-α (0, 3.12, 6.25, 12.5, 25, 50, 100, 200 uM). Cell viability and migration were examined. Cell viability was determined MTT assay and cell migration was determined by wound healing assay. Caco-2 cells were treated with for 24 h, 48 h and 72 h incubation. There is a significant decrease was observed at 50, 100 and 200 µM for 48 h and 72 h incubation on cell viability in the MTT assay. Wound healing method observed decrease on migration at 12, 5, 25 and 50 µM in 24 h. These results suggest that tocopherol-α have promising antiproliferative effect on cell viability for research on cancer.
Keywords: Antiproliferative effect; Caco-2 cell line; tocopherol-α.
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
MDPI and ACS Style
Gök, A.; Kurtdede, E.; Cengiz, R.S.; Kısmalı, G.; Sel, T. The Effect of Tocopherol-α on the Cell Viability in Caco-2 Cell Line. Proceedings 2017, 1, 1062. https://doi.org/10.3390/proceedings1101062
AMA Style
Gök A, Kurtdede E, Cengiz RS, Kısmalı G, Sel T. The Effect of Tocopherol-α on the Cell Viability in Caco-2 Cell Line. Proceedings. 2017; 1(10):1062. https://doi.org/10.3390/proceedings1101062
Chicago/Turabian StyleGök, Ayşenur, Efe Kurtdede, Remzi Soner Cengiz, Görkem Kısmalı, and Tevhide Sel. 2017. "The Effect of Tocopherol-α on the Cell Viability in Caco-2 Cell Line" Proceedings 1, no. 10: 1062. https://doi.org/10.3390/proceedings1101062
