The Effects of Benzoxasol Derivate Compounds in Breast Cancer Cells ^†

Abstract

Breast cancer today is the most frequent cancer among women, and the second most common cause of cancer deaths among women. The aim of this study was to synthesize a new benzoxazole derivative, scan it for anti-cancer potential by MTT test using different breast cancer cell lines, and examine its effects on NF-κB and apoptosis related proteins by the western blot method. A newly synthesized benzoxazole derived compound was applied to cancer cell lines and its cytotoxicity was measured quantitatively by MTT test. Later, the level of its effects on NF-κB and apoptosis related proteins were examined. The structure of the compound synthesized in our study (5-amino-2-(p-bromobenzyl) benzoxazoleand 5-[4-chlorobutanamido]-2-(p-methylphenyl) benzoxazole were proved by elemental analysis. In the assay of the proteins by western, when heterocyclic compounds were added to the MDA and MCF-7 cell line, there was no difference from the control group in Apaf-1 and BCL-2 levels, but a reduction was observed in caspase and NF-κB levels compared with the control group. It is seen that this newly synthesized heterocyclic compound increases apoptosis by reducing the activation of NF-κB, and in this way has shown an effect of inhibiting tumor growth in cancer treatment.