The Importance of Entomo-Virological Investigation of Yellow Fever Virus to Strengthen Surveillance in Brazil
Abstract
:1. Introduction
2. Material and Methods
2.1. Mosquitoes Collection and Taxonomic Identification
2.2. Mosquitoes Maceration
2.3. RNA Extraction
2.4. Real-Time Reverse Transcription Polymerase Chain Reaction (RT-qPCR)
2.5. Nucleotide Sequencing
2.6. Bioinformatic Analysis
3. Results
3.1. Collection and Taxonomic Identification
3.2. RT-qPCR Detection
3.3. Phylogenetic Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample ID | Species | Specimens Per Pool | Location | Collection Date | RT-qPCR Ct Value |
---|---|---|---|---|---|
AR831906 | Hg. janthinomys | 30 | Jandaia—GO | 24-Sep.-2016 | 26.02/26.31 |
AR831907 | Hg. janthinomys | 30 | 24-Sep.-2016 | 23.05/23.98 | |
AR831908 | Hg. janthinomys | 28 | 24-Sep.-2016 | 20.99/21.53 | |
AR831909 | Hg. janthinomys | 7 | 26-Sep.-2016 | 18.19/18.35 | |
AR831914 | Sa. glaucodaemon | 30 | 24-Sep.-2016 | 31.05/31.49 | |
AR843690 | Ae. albopictus | 25 | Ituêta—MG | 14-Jan.-2017 | 23.82/23.93 |
AR843692 | Ae. scapularis | 25 | 31.08/31.08 | ||
AR843693 | Ae. argyrothorax | 2 | 32.83/34.74 | ||
AR843713 | Hg. janthinomys | 15 | Central de Minas—MG | 13-Jan.-2017 | 32.07/32.40 |
AR843715 | Ae. albopictus ♀ | 4 | Alvarenga—MG | 15-Jan.-2017 | 33.49/35.06 |
AR843716 | Ae. argyrothorax | 2 | 32.44/33.31 | ||
AR843717 | Aedes sp. | 1 | 35.14/35.95 | ||
AR843720 | Hg. janthinomys | 25 | 28.95/29.20 | ||
AR843721 | Hg. janthinomys | 25 | 28.33/28.55 | ||
AR843728 | Hg. janthinomys | 19 | São José do Jacuri—MG | 16-Jan.-2017 | 31.86/32.32 |
AR843738 | Ae. scapularis | 3 | São Sebastião do Maranhão—MG | 17-Jan.-2017 | 35.03/35.12 |
AR843741 | Hg. leucocelaenus | 2 | 24.07/24.10 | ||
AR843745 | Ae. serratus | 1 | Itamarandiba—MG | 18-Jan.-2017 | 35.11/35.80 |
AR843765 | Hg. janthinomys | 6 | Felício dos Santos—MG | 20-Jan.-2017 | 32.43/32.59 |
AR843771 | Ae. albopictus ♀ | 2 | Caratinga—MG | 10-Jan.-2017 | 34.96/35.28 |
AR843772 | Ae. serratus | 2 | 31.78/32.54 | ||
AR843777 | Hg. janthinomys | 1 | 27.60/27.60 | ||
AR843807 | Hg. janthinomys | 4 | Imbé de Minas—MG | 16-Jan.-2017 | 34.52/35.09 |
AR843821 | Ae. albopictus ♀ | 1 | São Domingos das Dores—MG | 19-Jan.-2017 | 35.46/35.75 |
AR843829 | Ae. albopictus ♀ | 4 | 35.12/35.25 | ||
AR845803 | Hg. janthinomys | 18 | Cocos—BA | 17,18,20-Mar.-2017 | 28.62/28.64 |
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Cruz, A.C.R.; Hernández, L.H.A.; Aragão, C.F.; da Paz, T.Y.B.; da Silva, S.P.; da Silva, F.S.; de Aquino, A.A.; Cereja, G.J.G.P.; Nascimento, B.L.S.d.; Rosa Junior, J.W.; et al. The Importance of Entomo-Virological Investigation of Yellow Fever Virus to Strengthen Surveillance in Brazil. Trop. Med. Infect. Dis. 2023, 8, 329. https://doi.org/10.3390/tropicalmed8060329
Cruz ACR, Hernández LHA, Aragão CF, da Paz TYB, da Silva SP, da Silva FS, de Aquino AA, Cereja GJGP, Nascimento BLSd, Rosa Junior JW, et al. The Importance of Entomo-Virological Investigation of Yellow Fever Virus to Strengthen Surveillance in Brazil. Tropical Medicine and Infectious Disease. 2023; 8(6):329. https://doi.org/10.3390/tropicalmed8060329
Chicago/Turabian StyleCruz, Ana Cecília Ribeiro, Leonardo Henrique Almeida Hernández, Carine Fortes Aragão, Thito Yan Bezerra da Paz, Sandro Patroca da Silva, Fábio Silva da Silva, Ana Alice de Aquino, Glennda Juscely Galvão Pereira Cereja, Bruna Lais Sena do Nascimento, José Wilson Rosa Junior, and et al. 2023. "The Importance of Entomo-Virological Investigation of Yellow Fever Virus to Strengthen Surveillance in Brazil" Tropical Medicine and Infectious Disease 8, no. 6: 329. https://doi.org/10.3390/tropicalmed8060329