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Article

A Practical Approach for Quantitative Polymerase Chain Reaction, the Gold Standard in Microbiological Diagnosis

by 1,2,†, 3,†, 1,2,* and 1,2
1
Department of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis utca 6, H-6725 Szeged, Hungary
2
MTA-SZTE Microbiology and Health Education Research Group, H-6720 Szeged, Hungary
3
Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Marcello Iriti
Received: 20 September 2021 / Revised: 5 November 2021 / Accepted: 7 January 2022 / Published: 17 January 2022
(This article belongs to the Special Issue Feature Papers 2021 Editors Collection)
From gene expression studies to identifying microbes, quantitative polymerase chain reaction (qPCR) is widely used in research and medical diagnostics. In transmittable diseases like the Ebola outbreak in West Africa (2014–2016), or the present SARS-CoV2 pandemic qPCR plays a key role in the detection of infected patients. Although the technique itself is decades old with reliable approaches (e.g., TaqMan assay) in the diagnosis of pathogens many people showed distrust in it during the SARS-CoV2 outbreak. This came mainly from not understanding or misunderstanding the principles of qPCR. This situation motivated us to design a simple laboratory practical class, in which students have opportunities to understand the underlying principles of qPCR and its advantages in microbiological diagnosis. Moreover, during the exercise, students can develop skills such as handling experimental assays, and the ability to solve problems, discuss their observations. Finally, this activity brings them closer to the clinical practice and they can see the impact of the science on real life. The class is addressed to undergraduate students of biological sciences. View Full-Text
Keywords: laboratory practical class; undergraduate teaching; qPCR laboratory practical class; undergraduate teaching; qPCR
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MDPI and ACS Style

Mosolygó, T.; Laczi, K.; Spengler, G.; Burián, K. A Practical Approach for Quantitative Polymerase Chain Reaction, the Gold Standard in Microbiological Diagnosis. Sci 2022, 4, 4. https://doi.org/10.3390/sci4010004

AMA Style

Mosolygó T, Laczi K, Spengler G, Burián K. A Practical Approach for Quantitative Polymerase Chain Reaction, the Gold Standard in Microbiological Diagnosis. Sci. 2022; 4(1):4. https://doi.org/10.3390/sci4010004

Chicago/Turabian Style

Mosolygó, Tímea, Krisztián Laczi, Gabriella Spengler, and Katalin Burián. 2022. "A Practical Approach for Quantitative Polymerase Chain Reaction, the Gold Standard in Microbiological Diagnosis" Sci 4, no. 1: 4. https://doi.org/10.3390/sci4010004

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