Sci

The increasing demand for industrial resource optimization has driven the creation of integrated methodologies for the technical assessment of complex operations such as gas oil hydrocracking. This study examines the technical performance of a mass and energy-integrated gas oil hydrocracking process using the Extended Water–Energy–Product (E-WEP) methodology, which enables the quantification of 12 key indicators related to water, energy, and raw material usage. The research addresses the challenge of high demineralized water consumption in conventional hydrocracking processes. The findings show a production yield of 95.77% and a recycled hydrogen reuse rate of 67.99%, expressed as the Index of Reused Unconverted Material (IRUM). In terms of water use, fresh water demand decreased to 26.99 m³/h and wastewater discharge to 21 m³/h, although 77.79% of the total water processed is released as effluent, corresponding to the Wastewater Production Ratio (WPR). From the energy standpoint, total energy consumption increased to 2966.57 MMBTU/h, primarily due to the use of additional electrical equipment for mass integration. The Total Cost of Energy (TCE) reached 3,563,840.10 USD/day, with electricity (1630.82 kWh/t) as the dominant source, negatively influencing the process’s economic efficiency. Despite this energy drawback, the evaluated configuration achieves the most sustainable water use compared to conventional and integrated PVC production schemes, underscoring the importance of adopting holistic evaluations that jointly address technical efficiency, environmental impact, and economic feasibility.

Antimicrobial resistance (AMR) is a major public health threat that urgently requires alternative strategies to address this challenge. Klebsiella spp. are among the most important clinical pathogens and a leading cause of opportunistic nosocomial infections, with high morbidity and mortality associated with strains resistant to last-line antimicrobials such as carbapenems. Bacteriophages are considered a promising therapeutic option for treating infections caused by Klebsiella strains. Hence, the aim of this work was to isolate and characterize a phage capable of infecting carbapenem-resistant Klebsiella strains. The phage KpCCP1 was isolated using the double layer agar method (DLA), from the influent of a wastewater treatment plant, which was characterized through phenotypic and genomic analyses. Morphological characteristics were determined using TEM, and its host range was evaluated against a collection of 133 Klebsiella strains. Its whole genome was sequenced using the Illumina NovaSeq X Plus platform and then assembled and annotated. VICTOR was used for phylogenetic analysis of the isolated phage, and VIRIDIC to compare its genome with those of its closest relatives. KpCCP1 is a tailed dsDNA lytic phage with a genome size of 177,276 bp and a GC content of 41.82%. It encodes 292 ORFs, including two tRNA genes. Phage KpCCP1 is a member of the Slopekvirus genus in the Straboviridae family. It is capable of infecting 22 carbapenem-resistant Klebsiella strains, including K. pneumoniae and K. michiganensis. Notably, it does not contain virulence or antibiotic resistance genes and harbors putative anti-CRISPR genes, therefore representing a promising candidate for phage therapy against clinically critical Klebsiella strains.

Citrus fruit processing, mainly for fresh juice production in the food industry, generates significant amounts of residues and by-products enriched with bioactive components. Peels are the primary waste fraction of citrus fruits, along with discarded pulp and seeds. This study aimed to identify the most fast and sustainable extraction process for flavonoids on a laboratory scale by varying the solvent and extraction methodology, and comparing the yields in order to evaluate their influence on total and individual flavonoid content. A chromatographic analysis was also performed using ultrahigh-performance liquid chromatography (UHPLC) with a 10 min run time. Our focus was on selecting the most user-friendly and cost-effective methodology. Ultrasound- and microwave-assisted extraction equipment were used with green solvents (water and ethanol) and compared for their efficiency in recovering flavonoid compounds from a mixture of peel and pulp. For this study, two widely cultivated Mediterranean citrus varieties were selected: ‘Marsh’ seedless grapefruits (Citrus paradisi Macf.) and ‘Comun’ mandarins (C. deliciosa Ten.). Lab-scale extraction results showed that ultrasound-assisted extraction with a simple ultrasonic bath, using an ethanol–water mixture provided the highest total flavonoid recovery and improved the extraction of key flavanones such as hesperidin, narirutin, and naringin. All ethanol–water mixtures tested (1:1, 7:3, and 3:7) yielded higher flavonoid levels in grapefruit (approximately 2500 mg/100 g DW) and mandarin (approximately 1200 mg/100 g DW) wastes compared with water or ethanol alone. This method offers a scalable and green strategy for valorizing citrus residues.