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Article
Peer-Review Record

Nucleolin Interacts and Co-Localizes with Components of Pre-Catalytic Spliceosome Complexes

by Iva Ugrinova 1,†, Mounira Chalabi-Dchar 2,†, Karine Monier 2 and Philippe Bouvet 2,3,*
Reviewer 1:
Reviewer 2:
Submission received: 4 June 2019 / Accepted: 25 June 2019 / Published: 1 July 2019
Version 1
DOI: 10.3390/sci1020033

Round 1

Reviewer 1 Report

It is an interesting study with insightful data on NCL role in co-transcriptional splicing mechanism. There are minor revisions will improve the paper effectively.

Comments for author File: Comments.pdf

Reviewer 2 Report

Review of the manuscript by Ugrinova et al, “Nucleolin Interacts and Co-Localizes with Components of Pre-Catalytic Spliceosome Complexes (Version 1, Original)”

This is a nice study suggesting novel functions for the predominantly nucleolar phosphoprotein nucleolin in the nucleus and in the regulation of co-transcriptional splicing of RNA polymerase II products. The experiments cover a range of methods to support the central hypothesis, from co-localization and co-immunoprecipitation of splicosomal components with nucleolin up to functional assays after knocking down nucleolin. I would recomend acceptance of the study after minor revisions that should address the following points:

  1. Characterization of the acetyl-nucleolin antibody, an IF picture with peptide competition would strengthen the interpretation of the results
  2. Fig 3. A vice versa experiment with immunoprecipitation of splicing factors and co-IP of nucleolin would be nice, but not absolutely required. What is necessary is that the experiment was performed at least 3 times with the same result.
  3. Fig 4. D/E here only two experiments were used to do statistics, this is not acceptable for publication, one more experiment is necessary

This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.


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