Nucleolin Interacts and Co-Localizes with Components of Pre-Catalytic Spliceosome Complexes
Round 1
Reviewer 1 Report
It is an interesting study with insightful data on NCL role in co-transcriptional splicing mechanism. There are minor revisions will improve the paper effectively.
Comments for author File: 
Comments.pdf
Reviewer 2 Report
Review of the manuscript by Ugrinova et al, “Nucleolin Interacts and Co-Localizes with Components of Pre-Catalytic Spliceosome Complexes (Version 1, Original)”
This is a nice study suggesting novel functions for the predominantly nucleolar phosphoprotein nucleolin in the nucleus and in the regulation of co-transcriptional splicing of RNA polymerase II products. The experiments cover a range of methods to support the central hypothesis, from co-localization and co-immunoprecipitation of splicosomal components with nucleolin up to functional assays after knocking down nucleolin. I would recomend acceptance of the study after minor revisions that should address the following points:
- Characterization of the acetyl-nucleolin antibody, an IF picture with peptide competition would strengthen the interpretation of the results
- Fig 3. A vice versa experiment with immunoprecipitation of splicing factors and co-IP of nucleolin would be nice, but not absolutely required. What is necessary is that the experiment was performed at least 3 times with the same result.
- Fig 4. D/E here only two experiments were used to do statistics, this is not acceptable for publication, one more experiment is necessary
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
