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Review

LC–NMR for Natural Product Analysis: A Journey from an Academic Curiosity to a Robust Analytical Tool

1
Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia
2
Institute of Applied Synthetic Chemistry, Vienna University of Technology, Getreidemarkt 9/163-AC, A-1060 Vienna, Austria
3
Department of Chemistry, Otago University, P.O. Box 56, Dunedin 9054, New Zealand
*
Author to whom correspondence should be addressed.
Received: 8 April 2019 / Revised: 17 December 2020 / Accepted: 24 December 2020 / Published: 6 January 2021
Liquid chromatography (LC)–nuclear magnetic resonance (NMR) combines the advantage of the outstanding separation power of liquid chromatography (LC) and the superior structural elucidating capability of nuclear magnetic resonance (NMR). NMR has proved that it is a standout detector for LC by providing maximum structural information about plant originated extracts, particularly on the isolating ability of isomeric (same molecular formula) and/or isobaric (same molecular weight) compounds as compared to other detectors. The present review provides an overview of the developmental trends and application of LC–NMR in natural product analysis. The different LC–NMR operational modes are described, and how technical improvements assist in establishing this powerful technique as an important analytical tool in the analysis of complex plant-derived compounds is also highlighted. On-flow, stop-flow and loop-storage modes, as well as the new offline mode LC–solid phase extraction (SPE)–NMR and capillary LC (capLC)–NMR configurations which avoid the ingestion of expensive deuterated solvents throughout the experiment, are mentioned. Utilization of cryogenic probe and microprobe technologies, which are the other important promising approaches for guaranteeing sensitivity, are also described. Concluding remarks and future outlooks are also discussed. View Full-Text
Keywords: separation technique; spectroscopic technique; hyphenated techniques; LC–NMR; natural products separation technique; spectroscopic technique; hyphenated techniques; LC–NMR; natural products
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MDPI and ACS Style

Gebretsadik, T.; Linert, W.; Thomas, M.; Berhanu, T.; Frew, R. LC–NMR for Natural Product Analysis: A Journey from an Academic Curiosity to a Robust Analytical Tool. Sci 2021, 3, 6. https://doi.org/10.3390/sci3010006

AMA Style

Gebretsadik T, Linert W, Thomas M, Berhanu T, Frew R. LC–NMR for Natural Product Analysis: A Journey from an Academic Curiosity to a Robust Analytical Tool. Sci. 2021; 3(1):6. https://doi.org/10.3390/sci3010006

Chicago/Turabian Style

Gebretsadik, Tesfay; Linert, Wolfgang; Thomas, Madhu; Berhanu, Tarekegn; Frew, Russell. 2021. "LC–NMR for Natural Product Analysis: A Journey from an Academic Curiosity to a Robust Analytical Tool" Sci 3, no. 1: 6. https://doi.org/10.3390/sci3010006

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