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Open AccessArticlePost Publication Peer ReviewVersion 1

Nucleolin Interacts and Co-Localizes with Components of Pre-Catalytic Spliceosome Complexes (Version 1)

1
“Roumen Tsanev” Institute of Molecular Biology, Bulgarian Academy of Sciences, BG1113 Sofia, Bulgaria
2
Cancer Cell Plasticity Department, Centre de Recherche en Cancérologie de Lyon, Université de Lyon, UMR INSERM 1052 CNRS 5286, Centre Léon Bérard, Lyon 69008, France
3
Ecole Normale Supérieure de Lyon, Université de Lyon, Lyon 69342, France
*
Author to whom correspondence should be addressed.
Authors contributed equally to this work.
Received: 4 June 2019 / Accepted: 25 June 2019 / Published: 1 July 2019
Peer review status: 1st round review Read review reports Apply as reviewer

Reviewer 1 Anjana Saxena Brooklyn College and The Graduate Center (City University of New York) Reviewer 2 Mansi Arora University of Colorado Boulder Reviewer 3
Version 1 Approved with revisions Agreed, preparing report Reviewer inviting
Nucleolin is an RNA binding protein that is involved in many post-transcriptional regulation steps of messenger RNAs in addition to its nucleolar role in ribosomal RNA transcription and assembly in pre-ribosomes. Acetylated nucleolin was found to be associated with nuclear speckles and to co-localize with the splicing factor SC35. Previous nuclear pull down of nucleolin identified several splicing components and factors involved in RNA polymerase II transcription associated with nucleolin. In this report, we show that these splicing components are specifics of the pre-catalytic A and B spliceosomes, while proteins recruited in the Bact, C and P complexes are absent from the nucleolin interacting proteins. Furthermore, we show that acetylated nucleolin co-localized with P-SF3B1, a marker of co-transcriptional active spliceosomes. P-SF3B1 complexes can be pulled down with nucleolin specific antibodies. Interestingly, the alternative splicing of Fibronectin at the IIICS and EDB sites was affected by nucleolin depletion. These data are consistent with a model where nucleolin could be a factor bridging RNA polymerase II transcription and assembly of pre-catalytic spliceosome similarly to its function in the co-transcriptional maturation of pre-rRNA. View Full-Text
Keywords: nucleolin; spliceosome assembly; splicing factors; immunofluorescence; proteomic nucleolin; spliceosome assembly; splicing factors; immunofluorescence; proteomic
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Ugrinova, I.; Chalabi-Dchar, M.; Monier, K.; Bouvet, P. Nucleolin Interacts and Co-Localizes with Components of Pre-Catalytic Spliceosome Complexes. Sci 2019, 1, 33.

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1

Reviewer 1

Sent on 14 Aug 2019 by Anjana Saxena | Approved with revisions
Brooklyn College and The Graduate Center (City University of New York)

It is an interesting study with insightful data on NCL role in co-transcriptional splicing mechanism. There are minor revisions will improve the paper effectively.

Reviewer 2

Mansi Arora | Agreed, preparing report
University of Colorado Boulder

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