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J. Funct. Morphol. Kinesiol. 2018, 3(1), 2; https://doi.org/10.3390/jfmk3010002

Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine

1
G.E.R.N. Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center—Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106 Freiburg, Germany
2
Department of Biomedical Engineering, University of Michigan, Michigan, MI 48109, USA
*
Author to whom correspondence should be addressed.
Received: 14 November 2017 / Revised: 11 December 2017 / Accepted: 13 December 2017 / Published: 22 December 2017
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Abstract

In a worldwide effort to generate clinically useful therapeutic or preventive interventions, harnessing biophysical stimuli for directing cell fate is a powerful strategy. With the vision to control cell function through engineering cell shape, better understanding, measuring, and controlling cell shape for ultimately utilizing cell shape-instructive materials is an emerging “hot” topic in regenerative medicine. This review highlights how quantitation of cellular morphology is useful not only for understanding the effects of different microenvironmental or biophysical stimuli on cells, but also how it could be used as a predictive marker of biological responses, e.g., by predicting future mesenchymal stromal cell differentiation. We introduce how high throughput image analysis, combined with computational tools, are increasingly being used to efficiently and accurately recognize cells. Moreover, we discuss how a panel of quantitative shape descriptors may be useful for measuring specific aspects of cellular and nuclear morphology in cell culture and tissues. This review focuses on the mechano-biological principle(s) through which biophysical cues can affect cellular shape, and recent insights on how specific cellular “baseline shapes” can intentionally be engineered, using biophysical cues. Hence, this review hopes to reveal how measuring and controlling cellular shape may aid in future regenerative medicine applications. View Full-Text
Keywords: cell morphology; cell shape; biophysical cues; tissue engineering; cell imaging; quantitative analysis; engineering cell morphology; predicting phenotypic outcomes; morphological signatures; morphological fingerprints; mesenchymal stromal cells cell morphology; cell shape; biophysical cues; tissue engineering; cell imaging; quantitative analysis; engineering cell morphology; predicting phenotypic outcomes; morphological signatures; morphological fingerprints; mesenchymal stromal cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Hart, M.L.; Lauer, J.C.; Selig, M.; Hanak, M.; Walters, B.; Rolauffs, B. Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine. J. Funct. Morphol. Kinesiol. 2018, 3, 2.

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J. Funct. Morphol. Kinesiol. EISSN 2411-5142 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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