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Article

Transcriptome Analysis of the Immune Process of Golden Pompano (Trachinotus ovatus) Infected with Streptococcus agalactiae

by
Jie Gao
1,2,†,
Hua-Yang Guo
1,3,†,
Ming-Jian Liu
1,
Ke-Cheng Zhu
1,3,
Bo Liu
1,3,
Bao-Suo Liu
1,3,
Nan Zhang
1,3,
Shi-Gui Jiang
1,3,4 and
Dian-Chang Zhang
1,3,4,*
1
Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
2
Ocean College, Hebei Agricultural University, Qinhuangdao 066000, China
3
Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China
4
Sanya Tropical Fisheries Research Institute, Sanya 572019, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Fishes 2023, 8(1), 52; https://doi.org/10.3390/fishes8010052
Submission received: 22 December 2022 / Revised: 8 January 2023 / Accepted: 9 January 2023 / Published: 13 January 2023
(This article belongs to the Special Issue Application of Transcriptomics in Fish)
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Abstract

The golden pompano (Trachinotus ovatus) is one of the most economically valuable marine fishes in South China. Streptococcus agalactiae, an infectious Gram-positive bacterium that is highly destructive for golden pompano culture, has recently caused massive losses to the golden pompano industry. This study aimed to investigate the dynamic immune response of golden pompano to S. agalactiae infection, using RNA-seq analysis at two different time points after infection. Abundances of differentially expressed genes (DEGs) gradually increased in the liver and spleen 48–120 h post-infection, whereas those in the head kidney were lower at 120 h than at 48 h. Pathway enrichment analysis of DEGs revealed that genes related to the complement system were continuously transcribed between 48 and 120 h. Metabolic and immune-regulation-related pathways were highly enriched in the liver 48 h after infection. Transcriptome analysis was verified using quantitative PCR for eight genes with similar expression trends. This study revealed the inflammatory response of golden pompano after S. agalactiae infection, including inflammation-related chemokines and signaling pathways. Our findings provide a theoretical basis for studying S. agalactiae resistance in golden pompano and provide a reliable resource for the genetic breeding of fish.
Keywords: Trachinotus ovatus; Streptococcus agalactiae; inflammation; immune responses; transcriptomics Trachinotus ovatus; Streptococcus agalactiae; inflammation; immune responses; transcriptomics
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MDPI and ACS Style

Gao, J.; Guo, H.-Y.; Liu, M.-J.; Zhu, K.-C.; Liu, B.; Liu, B.-S.; Zhang, N.; Jiang, S.-G.; Zhang, D.-C. Transcriptome Analysis of the Immune Process of Golden Pompano (Trachinotus ovatus) Infected with Streptococcus agalactiae. Fishes 2023, 8, 52. https://doi.org/10.3390/fishes8010052

AMA Style

Gao J, Guo H-Y, Liu M-J, Zhu K-C, Liu B, Liu B-S, Zhang N, Jiang S-G, Zhang D-C. Transcriptome Analysis of the Immune Process of Golden Pompano (Trachinotus ovatus) Infected with Streptococcus agalactiae. Fishes. 2023; 8(1):52. https://doi.org/10.3390/fishes8010052

Chicago/Turabian Style

Gao, Jie, Hua-Yang Guo, Ming-Jian Liu, Ke-Cheng Zhu, Bo Liu, Bao-Suo Liu, Nan Zhang, Shi-Gui Jiang, and Dian-Chang Zhang. 2023. "Transcriptome Analysis of the Immune Process of Golden Pompano (Trachinotus ovatus) Infected with Streptococcus agalactiae" Fishes 8, no. 1: 52. https://doi.org/10.3390/fishes8010052

APA Style

Gao, J., Guo, H.-Y., Liu, M.-J., Zhu, K.-C., Liu, B., Liu, B.-S., Zhang, N., Jiang, S.-G., & Zhang, D.-C. (2023). Transcriptome Analysis of the Immune Process of Golden Pompano (Trachinotus ovatus) Infected with Streptococcus agalactiae. Fishes, 8(1), 52. https://doi.org/10.3390/fishes8010052

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