A Genetic Assessment of Parentage in the Blackspot Sergeant Damselfish, Abudefduf sordidus (Pisces: Pomacentridae)
Abstract
:1. Introduction
2. Results
2.1. Microsatellites
2.2. Nesting and Spawning Activity
2.3. Genetic Paternity
2.4. Genetic Maternity
3. Discussion
3.1. Mating System
3.2. Male Reproductive Behavior
3.3. Female Reproductive Behavior
4. Materials and Methods
4.1. Nest Site Monitoring and Sample Collection
4.2. Microsatellite Methods
4.3. Genetic Parentage
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Locus | Primers | Repeat | Size Range |
---|---|---|---|
D123 | F: HEX-caacgaacaaaaggcaaacaaa | (TAGA)n | 128–180 |
R: cactattaaccccagcaagacc | |||
D113 | F: 6-FAM-caaggttaccaagcgtgaaatg | (TAGA)n | 192–232 |
R: attcgtggttttccttcatcag | |||
D112 | F: HEX-atggacaccacgctagtattac | (TAGA)n | 141–181 |
R: caagttggctgaatgtctgctg | |||
D7 | F: 6-FAM-caggaggcattagtcatcaaag | (TAGA)n | 196–228 |
R: tatagcacccagtaattacaag | |||
C9 | F: HEX-cagatgtttgatggagagatac | (CATC)n | 164–220 |
R: agcagaaccaaacagtccaagc | |||
D102 | F: 6-FAM-aaatctcagcagcaaacatctc | (TAGA)n | 272–312 |
R: tgtccgtcctcctcccatcaga |
Locus | Number of Alleles | HO | HE | NE | NE | HW | F (Null) |
---|---|---|---|---|---|---|---|
1-P a | 2-P | ||||||
D123- | 10 | 0.845 | 0.797 | 0.579 | 0.402 | * | −0.0356 |
D113- | 10 | 0.798 | 0.839 | 0.496 | 0.325 | NS | 0.0262 |
D112- | 8 | 0.738 | 0.803 | 0.568 | 0.390 | NS | 0.0439 |
D7- | 9 | 0.738 | 0.791 | 0.589 | 0.411 | NS | 0.0293 |
C9- | 5 | 0.655 | 0.681 | 0.763 | 0.610 | NS | 0.0171 |
D102- | 7 | 0.726 | 0.683 | 0.732 | 0.562 | NS | −0.0402 |
Mean | 8.2 | 0.054 + | 0.007 + |
Male ID | Nest Cycles | Clutches Spawned | Clutches Analyzed | Clutches Parasitized | Offspring Sired by Male | Offspring Not Sired by Male | Alloparental Care (%) | Number of Females |
---|---|---|---|---|---|---|---|---|
F2 | 7 | 14 | 14 | 3 | 105 | 12 | 11 | 9 |
N | 8 | 15 | 15 | 3 | 115 | 8 | 7 | 11 |
Pipe | 2 | 4 | 4 | 0 | 27 | 0 | 0 | 4 |
E1 | 6 | 15 | 14 | 0 | 102 | 0 | 0 | 10 |
B/1 | 8 | 16 | 16 | 5 | 106 | 10 | 9 | 10 |
F1 | 6 | 12 | 11 | 1 | 79 | 2 | 4 | 8 |
H/Z | 9 | 19 | 18 | 1 | 141 | 2 | 1 | 14 * |
Ch1 | 6 | 11 | 11 | 2 | 96 | 5 | 5 | 8 |
K | 9 | 17 | 13 | 2 | 103 | 8 | 8 | 9 |
L | 7 | 15 | 12 | 2 | 98 | 4 | 4 | 9 |
Total | 68 | 138 | 129 | 19 | 974 | 51 | 5 @ | 74 # |
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Kerr Lobel, L.; Drown, D.M.; Barber, P.H.; Lobel, P.S. A Genetic Assessment of Parentage in the Blackspot Sergeant Damselfish, Abudefduf sordidus (Pisces: Pomacentridae). Fishes 2019, 4, 53. https://doi.org/10.3390/fishes4040053
Kerr Lobel L, Drown DM, Barber PH, Lobel PS. A Genetic Assessment of Parentage in the Blackspot Sergeant Damselfish, Abudefduf sordidus (Pisces: Pomacentridae). Fishes. 2019; 4(4):53. https://doi.org/10.3390/fishes4040053
Chicago/Turabian StyleKerr Lobel, Lisa, Devin M. Drown, Paul H. Barber, and Phillip S. Lobel. 2019. "A Genetic Assessment of Parentage in the Blackspot Sergeant Damselfish, Abudefduf sordidus (Pisces: Pomacentridae)" Fishes 4, no. 4: 53. https://doi.org/10.3390/fishes4040053