Changes in Sex Steroids and Ovarian Steroidogenic Enzyme mRNA Levels in Artificially Maturing Japanese Eel (Anguilla japonica) and Naturally Maturing New Zealand Longfin Eel (Anguilla dieffenbachii) during Vitellogenesis
Abstract
:1. Introduction
2. Results
2.1. Gonadosomatic Indices in Japanese and Longfin Eels
2.2. Serum Steroid Hormone Levels in Japanese and Longfin Eels
2.3. Isolation of Five Steroidogenic Enzyme cDNA Fragments from Longfin Eel Ovary
2.4. Expression of Ovarian Steroidogenic Enzymes in Japanese and Longfin Eels
3. Discussion
4. Materials and Methods
4.1. Animals and Sampling Design
4.1.1. Sampling of Japanese Eels
4.1.2. Sampling of Longfin Eels
4.2. Macroscopical Analyses
4.3. Radioimmunoassay
4.4. Isolation of Partial Steroidogenic Enzyme cDNAs from Longfin Eel Ovary
4.5. Real-Time Quantitative PCR (qPCR)
- sense
- 5’-TTAAGCGACAGAGGCAACCT-3’
- antisense
- 5’-CCAACTGTGTACTCCCCGAT-3
- probe
- 5'(FAM)-CCGTATCTTGAGGCCACCATCAGG-3'(TAMURA).
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Primer | Sequence |
---|---|---|
Cyp11a | sense | 5’-GTTCATCGTATCATGCTCAAC-3’ |
antisense | 5’-AGTCCACCTCTCCTATGTTG-3’ | |
hsd3b | sense | 5’-TGTGGCTTCCTGGGAGAGA-3’ |
antisense | 5’-GTACATCTACGGCGATGG-3 | |
cyp17a1 | sense | 5’-ATAGTGGACACCGTGGCCAAGGA-3 |
antisense | 5’-CTGCAGGACCACCCCAAATTTGC-3’ | |
hsd17b1 | sense | 5’-AGAGTGGTGCTGATCACG-3’ |
antisense | 5’-TGTGAGAGTTTGGCTATTCTC-3’ | |
cyp19a | sense | 5’-TGGCAGACAGTTCTGATCA-3’ |
antisense | 5’-AACCGAAGGGCTGGAAGAAA-3’ |
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Matsubara, H.; Lokman, P.M.; Kazeto, Y.; Okumura, H.; Ijiri, S.; Hirai, T.; Young, G.; Adachi, S.; Yamauchi, K. Changes in Sex Steroids and Ovarian Steroidogenic Enzyme mRNA Levels in Artificially Maturing Japanese Eel (Anguilla japonica) and Naturally Maturing New Zealand Longfin Eel (Anguilla dieffenbachii) during Vitellogenesis. Fishes 2019, 4, 52. https://doi.org/10.3390/fishes4040052
Matsubara H, Lokman PM, Kazeto Y, Okumura H, Ijiri S, Hirai T, Young G, Adachi S, Yamauchi K. Changes in Sex Steroids and Ovarian Steroidogenic Enzyme mRNA Levels in Artificially Maturing Japanese Eel (Anguilla japonica) and Naturally Maturing New Zealand Longfin Eel (Anguilla dieffenbachii) during Vitellogenesis. Fishes. 2019; 4(4):52. https://doi.org/10.3390/fishes4040052
Chicago/Turabian StyleMatsubara, Hajime, P. Mark Lokman, Yukinori Kazeto, Hiromi Okumura, Shigeho Ijiri, Toshiaki Hirai, Graham Young, Shinji Adachi, and Kohei Yamauchi. 2019. "Changes in Sex Steroids and Ovarian Steroidogenic Enzyme mRNA Levels in Artificially Maturing Japanese Eel (Anguilla japonica) and Naturally Maturing New Zealand Longfin Eel (Anguilla dieffenbachii) during Vitellogenesis" Fishes 4, no. 4: 52. https://doi.org/10.3390/fishes4040052