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Comparison of Sample Preparation Methods for Multielements Analysis of Olive Oil by ICP-MS

1
Tsukuba Life Science Innovation (T-LSI) Program, School of Integrative and Global Majors, University of Tsukuba, 1-1-1 Tennodai, Ibaraki 305-8577, Japan
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Institute of Agro-Environmental Sciences, NARO, 3-1-3 Kannondai Tsukuba, Ibaraki 305-0856, Japan
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Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan
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Environmental Engineering and Ecotechnology Laboratory (LGEET), National School of Engineers of Sfax (ENIS), University of Sfax, Route de Soukra Km 4 P.O. Box 1173, Sfax 3038, Tunisia
*
Author to whom correspondence should be addressed.
Methods Protoc. 2019, 2(3), 72; https://doi.org/10.3390/mps2030072
Received: 30 June 2019 / Revised: 27 July 2019 / Accepted: 15 August 2019 / Published: 19 August 2019
Elemental analysis of olive oils by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) is challenging because of the high organic load in olive oil samples and the low analyte concentrations. However, conflicting operating procedures in the preparation of oils prior to analysis by ICP-MS have been reported to overcome these difficulties. This study compared three methods of inorganic elements’ extraction from olive oils: The two commonly used microwave-assisted, acid digestion, and liquid–liquid, ultrasound-assisted extraction methods; and an optimized method: The combined microwave digestion-evaporation. Overall, microwave digestion-based methods did not compare opportunely, and ultrasound-assisted extraction was found to provide the best accord between simplicity of use, detection limits and precision improvement. The detection limits were in the range of 0.3–160 µg·kg-1, 0.012–190 µg·kg−1 and 0.00061–1.5 µg·kg−1, while repeatabilities were in the range of 5–21%, 5.4–99% and 5.1–40% for the microwave digestion, the combined digestion-evaporation and the ultrasound assisted extraction, respectively. The ultrasound-assisted extraction is therefore recommended as a preparation method for olive oils prior to analysis by ICP-MS. The broader range of elements that can be accurately detected is expected to help increase the discriminatory power and performance of geographical traceability models. View Full-Text
Keywords: multielements; olive oil; ICP-MS; microwave digestion; evaporation; ultrasound-assisted extraction multielements; olive oil; ICP-MS; microwave digestion; evaporation; ultrasound-assisted extraction
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MDPI and ACS Style

Damak, F.; Asano, M.; Baba, K.; Ksibi, M.; Tamura, K. Comparison of Sample Preparation Methods for Multielements Analysis of Olive Oil by ICP-MS. Methods Protoc. 2019, 2, 72. https://doi.org/10.3390/mps2030072

AMA Style

Damak F, Asano M, Baba K, Ksibi M, Tamura K. Comparison of Sample Preparation Methods for Multielements Analysis of Olive Oil by ICP-MS. Methods and Protocols. 2019; 2(3):72. https://doi.org/10.3390/mps2030072

Chicago/Turabian Style

Damak, Fadwa; Asano, Maki; Baba, Koji; Ksibi, Mohamed; Tamura, Kenji. 2019. "Comparison of Sample Preparation Methods for Multielements Analysis of Olive Oil by ICP-MS" Methods Protoc. 2, no. 3: 72. https://doi.org/10.3390/mps2030072

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