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Technical Note

Improved Detection and Fragmentation of Disulphide-Linked Peptides

by
Evelyne Maes
1,*,
Stefan Clerens
1,2,
Jolon M. Dyer
1,2,3,4 and
Santanu Deb-Choudhury
1
1
Food & Bio-Based Products, AgResearch Ltd., 1365 Springs Road, Lincoln 7674, New Zealand
2
Biomolecular Interaction Centre, University of Canterbury, Christchurch 8140, New Zealand
3
Riddet Institute, Massey University, Palmerston North 4442, New Zealand
4
Wine, Food & Molecular Biosciences, Lincoln University, Lincoln 7674, New Zealand
*
Author to whom correspondence should be addressed.
Methods Protoc. 2018, 1(3), 33; https://doi.org/10.3390/mps1030033
Submission received: 6 August 2018 / Revised: 24 August 2018 / Accepted: 28 August 2018 / Published: 3 September 2018
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Abstract

Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.
Keywords: disulphide bonds; MALDI-TOF/TOF MS; aniline; proof-of-existence disulphide bonds; MALDI-TOF/TOF MS; aniline; proof-of-existence
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MDPI and ACS Style

Maes, E.; Clerens, S.; Dyer, J.M.; Deb-Choudhury, S. Improved Detection and Fragmentation of Disulphide-Linked Peptides. Methods Protoc. 2018, 1, 33. https://doi.org/10.3390/mps1030033

AMA Style

Maes E, Clerens S, Dyer JM, Deb-Choudhury S. Improved Detection and Fragmentation of Disulphide-Linked Peptides. Methods and Protocols. 2018; 1(3):33. https://doi.org/10.3390/mps1030033

Chicago/Turabian Style

Maes, Evelyne, Stefan Clerens, Jolon M. Dyer, and Santanu Deb-Choudhury. 2018. "Improved Detection and Fragmentation of Disulphide-Linked Peptides" Methods and Protocols 1, no. 3: 33. https://doi.org/10.3390/mps1030033

APA Style

Maes, E., Clerens, S., Dyer, J. M., & Deb-Choudhury, S. (2018). Improved Detection and Fragmentation of Disulphide-Linked Peptides. Methods and Protocols, 1(3), 33. https://doi.org/10.3390/mps1030033

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