Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of MNP- and MNP@Ny6-Based Oligonucleotide Nanoprobes
2.2. Oligonucleotides Microextraction from Model Samples by MNP-Olig and MNP@Ny6-Olig Nanocomposites
3. Materials and Methods
3.1. Materials
3.2. Magnetic Nanocomposites Characterization
3.3. Synthesis and Isolation of Oligonucleotides
3.4. Magnetic Nanoparticles (MNP) Synthesis
3.5. Synthesis of Oligonucleotide-Modified MNPs (MNP-Olig)
3.6. Synthesis of MNP and Nylon 6 Nanocomposites (MNP@Ny6)
3.7. Synthesis of Oligonucleotide-Modified MNP@Ny6 (MNP@Ny6-Olig)
3.8. Colorimetric Biotin Detection
3.9. RNA Binding on MNP-Olig or MNP@Ny6-Olig Nanocomposites
3.10. RNA Release
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Description | 5′-Sequence-3′ | NA Type, Function |
---|---|---|
oligonucleotide-NH2 | GTCTTCCTTCTCCGCTT-Sp1-NH2 | DNA, capture probe |
FAM-oligonucleotide-NH2 | FAM-Sp2-GTCTTCCTTCTCCGCTT-Sp1-NH2 | |
T10-oligonucleotide | (T)10-GTCTTCCTTCTCCGCTT | |
T10-oligonucleotide-FAM * | (T)10-GTCTTCCTTCTCCGCTT-Sp2-FAM | |
T10-oligonucleotide-Biotin | (T)10-GTCTTCCTTCTCCGCTT-Sp3-Biotin | |
Sp1 Sp2 Sp3 |
Description | 5′-Sequence-3′ | NA Type, Function |
---|---|---|
oligonucleotide-NH2 No. 1 specific for RNA | GTCTTCCTTCTCCGCTT-Sp1-NH2 * | DNA, capture probe for MNP |
oligonucleotide-NH2 No. 2 non-specific for RNA | TAACCGATTTCAGATTT-Sp1-NH2 | |
T10-oligonucleotide No. 1 specific for RNA | (T)10-GTCTTCCTTCTCCGCTT | DNA, capture probe for MNP@Ny6 |
T10-oligonucleotide No. 2 non-specific for RNA | (T)10-TAACCGATTTCAGATTT | |
Target | Fam- GAGGCGAAGCGGAGAAGGAAGAC | RNA, target |
MNP Type | Capture DNA Probe Immobilization | Initial Target RNA Amount | Target RNA Binding | Target RNA Release | RNA Capture Efficiency |
---|---|---|---|---|---|
MNP | 50.0% | 0.30 nmol | 0.147 ± 0.015 nmol 49.0% a | 0.033 ± 0.010 nmol 22.4% b | 11.0% c |
MNP1%Ny6 | 73.3% | 0.180 ± 0.010 nmol 60.0% | 0.160 ± 0.013 nmol 88.9% | 53.3% | |
MNP5%Ny6 | 66.7% | 0.160 ± 0.018 nmol 53.3% | 0.087 ± 0.013 nmol 54.4% | 29.0% |
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Bulgakova, A.; Chubarov, A.; Dmitrienko, E. Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples. Magnetochemistry 2022, 8, 85. https://doi.org/10.3390/magnetochemistry8080085
Bulgakova A, Chubarov A, Dmitrienko E. Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples. Magnetochemistry. 2022; 8(8):85. https://doi.org/10.3390/magnetochemistry8080085
Chicago/Turabian StyleBulgakova, Anastasia, Alexey Chubarov, and Elena Dmitrienko. 2022. "Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples" Magnetochemistry 8, no. 8: 85. https://doi.org/10.3390/magnetochemistry8080085