Splicing and Expression Regulation of fruitless Gene in Bemisia tabaci (Hemiptera: Aleyrodidae)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insect Strains
2.2. Sample Collection
2.3. RNA Extraction and cDNA Synthesis
2.4. Gene Cloning and Splice-Variant Detection
2.5. Phylogenetic Analysis
2.6. Real-Time Quantitative PCR
2.7. RNA Interference
3. Results
3.1. Characterization of Btfru
3.2. Phylogenetic Analysis of Btfru
3.3. Developmental Expression of Btfru
3.4. Analyses of Alternative Splicing Variants of Btfru
3.5. Analysis of the Interaction between Bttra and Btfru
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Application of Primers | Gene Name | Primer Name | Primer Sequence (5′-3′) |
---|---|---|---|
Cloning and AS analysis | fru-a | fru6255-F | CGTCTCTCCCCCAACCAG |
fru-full-R | CCCTTAGCATCAATAGCGG | ||
fru-b | fru-F5-full | ATGGAGGAGGCATTTTGTTTGAAG | |
fru-R5-full | TTATGTGTTGTGCTTGAGCCTGAAA | ||
qRT-PCR analysis | fru-a | qfru-A-F1 | AAGCAATCCGCAGCCGTT |
qfru-A-R1 | CTGATGTCGTTGAGATACCGC | ||
fru-b | qfru-G-F2 | ATGAAAAACCACTTCTTGACGC | |
qfru-G-R2 | TATGTGTTGTGCTTGAGCCTGA | ||
fru | BTB-qF | CATTCGTCAAGTTTTTCGGGTA | |
BTB-qR | GGAAGGTCTCGCTCGCTAAA | ||
tra | dsTra-qF2 | AAGTCCCTCTCCTCAGCCCA | |
dsTra-qR2 | GCCACGGGTTAGACCTTTGA | ||
SDHA | SDHA-qF | GCGACTGATTCTTCTCCTGC | |
SDHA-qR | TGGTGCCAACAGATTAGGTGC | ||
RNAi analysis | EGFP | dsEGFP-F | TAATACGACTCACTATAGGGAGACAGTGCTTCAGCCGCTAC |
dsEGFP-R | TAATACGACTCACTATAGGGAGAGTTCACCTTGATGCCGTTC | ||
tra | dsTra-F2 | GGATCCTAATACGACTCACTATAGGTTGAGACGAATCAGCAATCG | |
dsTra-R3 | GGATCCTAATACGACTCACTATAGGGACCTTCGCAGGAACTTTTG |
Type | Variants | Exons Included | Size (bp) | Female (3) a | Male (3) a | Domain |
---|---|---|---|---|---|---|
fru-a | 1 | 1,2,3,4,5,6a,7 | 1263 | 3 | 3 | BTB + Zn |
2 | 1-198,160-7 | 278 | 1 | 0 | BTB | |
3 | 1-169,178-7 | 289 | 1 | 0 | BTB | |
4 | 1-210,110-7 | 316 | 1 | 0 | ||
5 | 1-194,88-7 | 354 | 1 | 0 | BTB | |
6 | 1-308,48-6a,7 | 357 | 1 | 0 | Zn | |
7 | 1-200,78-7 | 358 | 1 | 0 | BTB | |
8 | 1-279,71-6a,7 | 363 | 0 | 1 | Zn | |
9 | 1-161,85-7 | 390 | 1 | 0 | BTB | |
10 | 1-197,38-7 | 401 | 1 | 0 | BTB | |
11 | 1-132,98-7 | 406 | 1 | 0 | BTB | |
12 | 1-346,55-5,6a,7 | 444 | 1 | 0 | Zn | |
13 | 1-131,9-7 | 496 | 1 | 0 | BTB | |
14 | 1-305,37-5,6a,7 | 503 | 1 | 0 | ||
15 | 1,2,3-107,244-7 | 667 | 1 | 0 | BTB | |
16 | 1,2,3-7,79-7 | 895 | 0 | 1 | BTB | |
17 | 1,2,3,4,5-86,163-7 | 935 | 1 | 0 | BTB | |
18 | 1,2,3,4-73,21-6a,7 | 1074 | 0 | 1 | BTB + Zn | |
19 | 1,2,3,4,5,6a-66,82-7 | 1114 | 1 | 0 | BTB | |
20 | 1,2,3,4,5-56,7 | 1130 | 1 | 0 | BTB | |
21 | 1-10,98-2,3,4,5,6a,7 | 1155 | 1 | 0 | BTB + Zn | |
fru-b | 1 | 1,2,3,4,5,6b | 1143 | 3 | 3 | BTB + Zn |
2 | 1-185,155-6b | 253 | 1 | 0 | BTB | |
3 | 1-284,5,6b | 348 | 1 | 0 | ||
4 | 1-292,5,6b | 396 | 1 | 0 | BTB | |
5 | 1,2-81,178-6b | 517 | 0 | 1 | BTB | |
6 | 1-51,2,3,4,5,6b | 1090 | 1 | 0 | BTB + Zn |
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Liu, Y.; Xie, J.; Wang, W.; Lei, Y.; Zhou, X.; Zhang, Y.; Xie, W. Splicing and Expression Regulation of fruitless Gene in Bemisia tabaci (Hemiptera: Aleyrodidae). Horticulturae 2023, 9, 962. https://doi.org/10.3390/horticulturae9090962
Liu Y, Xie J, Wang W, Lei Y, Zhou X, Zhang Y, Xie W. Splicing and Expression Regulation of fruitless Gene in Bemisia tabaci (Hemiptera: Aleyrodidae). Horticulturae. 2023; 9(9):962. https://doi.org/10.3390/horticulturae9090962
Chicago/Turabian StyleLiu, Yating, Jinxi Xie, Wenlu Wang, Yanyuan Lei, Xuguo Zhou, Youjun Zhang, and Wen Xie. 2023. "Splicing and Expression Regulation of fruitless Gene in Bemisia tabaci (Hemiptera: Aleyrodidae)" Horticulturae 9, no. 9: 962. https://doi.org/10.3390/horticulturae9090962