BAP (6-Benzylaminopurine) Seed-Priming Enhanced Growth, Antioxidant Accumulation and Anthocyanin Metabolism in Olive Sprouts
, Ahlem Zrig 3,4,*, Mha Albqmi 2,5, Mohammad M. Al-Sanea 6, Taghreed S. Alnusaire 7, Mohammed S. Almuhayawi 8, Soad K. Al Jaouni 9, Shaimaa Hussein 10, Mona Warrad 11 and Hamada AbdElgawad 12Round 1
Reviewer 1 Report
The effects of BAP on the antioxidant activity and and anthocyanin metabolism in olive sprouts were investigated in this manuscript. It is very interesting and valuable for the industry. The design is very clear and easy to understand, two varieties selected with one concentration treated. However, some points should be clarified and responded. The details of comments as below,
1. The title, why did the authors address the lipid and water-soluble antioxidants? Is it essential? Can we use "antioxidants" only?
In abstract, the first sentence is not necessary, since it will make the readers confusing.
2. Some mistakes such as line 68, the more space before "Some" should be corrected. The authors should double-check the spelling and format mistakes. Line 207, 203, about the "p", they are not in the same stytle, capital or low case?
3. The reason of concentration selected was explained from line 100 to 105. What is the concentrations used in the citation, and what is the preliminary test?
4. Section 2.5, ascorbic acid as nutrient ingredient and water soluble antioxidant, have the authors monitored the changes?
5. Line 222, please confirm it is "Table 1", what is "alpha toco, beta-toco, and sigma" representing in table 1? And what is the small letters indicating? the same row or column? Where is the "d"? Maybe from the same row, right? then "Total" results, we couldn't understand the small letters with only "a, c". Similiar problems in table 2, and table 3. where is "d"?
6. Discussion section should be given based on your results.
7. The format in reference list is not uniform, for example, if it is correct about the title of article (capital in first letters) in line 439, then line 437 and 435, etc should be rivised.
8. The language should be improved.
Author Response
Dear reviewer
Reviewer 1
The effects of BAP on the antioxidant activity and and anthocyanin metabolism in olive sprouts were investigated in this manuscript. It is very interesting and valuable for the industry. The design is very clear and easy to understand, two varieties selected with one concentration treated. However, some points should be clarified and responded. The details of comments as below,
- The title, why did the authors address the lipid and water-soluble antioxidants? Is it essential? Can we use "antioxidants" only?
Response: Thanks for suggestion, we remove lipid and water-soluble.
In abstract, the first sentence is not necessary, since it will make the readers confusing.
Response: Thanks for suggestion, we rephrased the sentence
- Some mistakes such as line 68, the more space before "Some" should be corrected. The authors should double-check the spelling and format mistakes. Line 207, 203, about the "p", they are not in the same stytle, capital or low case?
Response: Thanks, we carefully removed all extra spaces and we wrote p uniformly.
- The reason of concentration selected was explained from line 100 to 105. What is the concentrations used in the citation, and what is the preliminary test?
Response: Thanks for your comments, more details on the preliminary experiment. The selection of a BAP concentration of 25 μM was selected by preliminary sprouting experiments, where various BAP concentrations (ranging from 0 to 50 μM) were assessed for their effects on the growth (fresh weight) and total antioxidant capacity (evaluated using the Ferric Reducing Antioxidant Power – (FRAP) assay) of olive sprouts.
- Section 2.5, ascorbic acid as nutrient ingredient and water soluble antioxidant, have the authors monitored the changes?
Response: Yes, we measured ascorbic acid, and the results were presented in Figure 4.
- Line 222, please confirm it is "Table 1",
Response: Thanks for critical observation, we change the order of the tables to fits with the sequence of their citation in the text.
what is "alpha toco, beta-toco, and sigma" representing in table 1?
Response: They are Alpha (α), beta (β) and gama (γ) tocopherols, more details were added for clarity.
And what is the small letters indicating? the same row or column? Where is the "d"? Maybe from the same row, right? then "Total" results, we couldn't understand the small letters with only "a, c". Similiar problems in table 2, and table 3. where is "d"?
Response: Different small letters in the same row indicate significant differences between the control and BAP treatment in the two varieties treatments (P < 0.05). Indeed in some tables the letters only, a, b or c. Thes mistakes checked and corrected.
- Discussion section should be given based on your results.
- The format in reference list is not uniform, for example, if it is correct about the title of article (capital in first letters) in line 439, then line 437 and 435, etc should be rivised.
Response: Thanks, we carefully checked and corrected the refences
- The language should be improved.
Response: We checked and improved the English language
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Reviewer 2 Report
The manuscript authored by Selim et al. described a comprehensive biochemical study on the role of BAP application in improving olive sprouts. It is well written with a clear presentation to close the knowledge gap of BAP priming effects on olive sprouting enhancement. I have a few minor comments.
The legends of table 1 and table 2 seem to be mistakenly placed. The positions of small letter denotations should be correctly described as there are no bars in all tables.
The last sentence of conclusion (Line 409-410) is not directly derived from the study of this research. It should be deleted or revised.
Minor editing of English language required.
Author Response
Dear reviewer
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Reviewer 3 Report
The paper is of medium interest, there are several studies on the same subject.
In the Material and methods chapter, point 2.2 is not clear how to prepare the samples. All applied chromatographic methods are very briefly described, so the experiment cannot be reproduced under identical conditions
n chapter 4.2, the involvement of BAP priming in the antioxidant activity of olive sprouts is not very clear
Chapter 5. Conclusion must be revised, it contains meaningless sentences
Author Response
Dear Reviewer
The paper is of medium interest, there are several studies on the same subject.
Response: Thanks, regarding you concern about the novelty of the work, here we aimed to explore the potential impact of benzylaminopurine (BAP) on enhancing the chemical composition and bioactivity of olive sprouts, highlighting the relative lack of comprehensive research on this innovative source of bioactive and nutritive compounds despite existing studies on olive fruits. Overall, there are several studies on olive fruits, there are no in-depth and comprehensive studies on olive sprouts as an innovative source of bioactive and nutritive compounds.
In the Material and methods chapter, point 2.2 is not clear how to prepare the samples. All applied chromatographic methods are very briefly described, so the experiment cannot be reproduced under identical conditions
Response: Thanks, we add more details on Applied chromatographic methods, we believed it well explained now.
In chapter 4.2, the involvement of BAP priming in the antioxidant activity of olive sprouts is not very clear
Response: Thanks, we rewrote the discussion sections including section 4.2 for more clarity
Chapter 5. Conclusion must be revised, it contains meaningless sentences
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Round 2
Reviewer 1 Report
The authors have corrected and improved the quality of manuscript. Now it can be considered to accept.
Author Response
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Reviewer 3 Report
the requested changes have been made
Author Response
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