VvPL11 Is a Key Member of the Pectin Lyase Gene Family Involved in Grape Softening

Round 1

Reviewer 1 Report

Dear authors,

Hope you are doing well.

I read your manuscript and I think that is interesting. However, I believe that needs a global and deep revision. Next, I indicate my comments for its improvement.

Major concerns:

- VvPL11. I think that the information in the sentence (line 18) is different of the Figure 5 graph. Please, review this sentence.

- Discussion. The discussion seems a result description. This section should be improved.

- Some reference cites (reference 41, line 108) does not match with the reference list. Please, review the list reference and text cites.

- Line 209. I can not understand this part. You used previous published data of transcriptome (Figure 5). However, you mention that 'your transcriptom'e... this information is not contained in the material and methods? Are your previous data? Moreover, the reference for previous transcriptome data (41; line 108) is not correct in the list of references. Please, review and explain this for a better understanding.

Minr concerns:

- Line 76. These grape clusters correspond to...?
- Some gene names do not showed with italics. For example, VvPL (line 83) and others. Please, review throught the text.

- Line 86-88. for what? Please, explain about this.

- Web pages citation. Web pages should include the date of the last access. Please, review citation instructions of the journal. Lines 95, 100, 102, 104, 111,...

- Line 117. The correct term is Reverese Transcriptase - quantitative PCR.

- Table S2. Please, include an additional column with amplicon size (bp)

- Line 145. Chr12 and chr18 do not appear in the Figure 1.

- Lines 148-149. I can not find this information in the Table S1.

- Line 150. Please, remove '2.'

- Line 151. FaPLc y PvPLa do not appear in this sentence but it is contained in the phylogenetic tree.

- Line 152. 'Subgroup'. You should mantain 'group' as well as in the Figure.

- Figure 2 legend. The meaning of red dot should be included in the legend.

- Figure 3 legend. I think that this Figure legend is wrong.

- Line 168. Please, indicate that this sequence has not been included in th Figure 3B and the reason or include a new multiplet alignment with this sequence.

- Line 170-172. Please, indicate in this part of the text that this analysis were performed from previous published data. Also, include the cite in the legend.

- Line 217. Why? Please, explain. Perhaps, you could include as supplementary figure.

- Line 238. Br+3d and Br+5d. This nomenclature is confuse because in the Figure appear as B5, B7...

Best regards,

Author Response

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Author Response File: Author Response.docx

Reviewer 2 Report

I. Introduction. Although the paper is one of molecular genetics, it is necessary to introduce some ideas related to the importance of the vine for Henan Province. Also, some data related to the economic impact of grape soaking in table and wine varieties would be welcome.

II. L.73 The phrase "commercial orchard" is totally inappropriate for the vine. Replace with the term "commercial vineyard".

III. 2.1 The research of the last decades shows that the expression of some genes is also influenced by environmental factors. Therefore, I consider useful a brief pedoclimatic description of the area where the plants subjected to molecular analyzes were grown.

IV. If there are differences between the expression of VvPL genes in different organs and vegetation periods in grapevine, it is assumed that there are also differences between different cultivars/species of grapevine. Why did the authors not consider it important to compare a vine variety with fruit that softens quickly with a vine variety in which the softening process is slowed down?

V. The DISCUSSION chapter is rather superficial. I suggest the authors to enrich it with other data from the literature that touch all the aspects addressed in the paper and to make the related comparisons.

VI. What is the practical purpose of this research? What are the next steps the authors propose for this study?

Author Response

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Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

Dear authors,

Thanks for your responses to my previous comments. On the one hand, I think that the manuscript has improved. However, I think that you should introduce some minor changes showed below. On the other hand, I continue have a major concern and it is the absence of the publication of raw transcriptome data that you are using in this manuscript.

1.- Please, change ‘ananass’ by ‘ananassa’.

2.- Line 299. Please, cite transcriptome data.

3.- Line 160. I think that Figure 1 legend is wrong. Please, change this legend.

4.- Lines 161-168. Authors constructed the phylogenetic tree with protein sequences. However, they refer to ‘genes’ in this paragraph. I consider that this should be modified.

5.-Line 127. Please, change ‘Reverese’ by ‘Reverse’.

6.- Response to Question 11. You mention that chr12 and chr18 not contain VvPL genes, however, this sentence is included: ‘Chromosome 1 contains four 154 VvPL members, while chromosomes 5, 7, 10, 12, 16, 18, 19 have one member each’. (lines 154-155). Please, review this sentence.

7.- Response to question 21. I think that this is correct but I think that this new figure showed in 'Author notes' should be included in the supplementary information. I can not find it.

 

Best regards,

 

 

 

 

 

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Round 3

Reviewer 1 Report

Dear authors,

Hope you are doing well. I think that the manuscript is situable for publication but I think you should introduce three minor changes:

- Line 155. Please, change '18' by '19' according to the Figure 1 or '19' by '18' in the Figure panel.

- Line 161. Please, separate legend of Figure 1 from the phylogeny description paragaph.

- Supplementary information. The supplementary table 2 has dissapeared from supplementary information.

Best regards,

 

Author Response

Please see the attachment.

Author Response File: Author Response.docx