Balancing Hormones and Gene Expressions for Rooting Success: Lovastatin Unveils Cytokinin Inhibition in Malus prunifolia var. ringo Apple Stem Cuttings
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Determination of Endogenous Hormone Content
2.3. RNA Extraction, cDNA Synthesis, and RT-qPCR Expression Analysis
2.4. Statistical Analysis
3. Results
3.1. Effect of 6-BA and Lovastatin Treatments on Adventitious Rooting and Endogenous Hormone Content
3.2. RT-qPCR Expression Analysis of CK Signal Transduction-Related Genes
3.3. RT-qPCR Expression Analysis of IAA-Related Genes
3.4. RT-qPCR Analysis of Root Development-Related Genes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sun, S.; Tahir, M.M.; Xie, Z.; Wei, P.; Yu, J.; Liu, H.; He, Y.; Ren, X.; Ma, Y.; Mao, J. Balancing Hormones and Gene Expressions for Rooting Success: Lovastatin Unveils Cytokinin Inhibition in Malus prunifolia var. ringo Apple Stem Cuttings. Horticulturae 2023, 9, 1341. https://doi.org/10.3390/horticulturae9121341
Sun S, Tahir MM, Xie Z, Wei P, Yu J, Liu H, He Y, Ren X, Ma Y, Mao J. Balancing Hormones and Gene Expressions for Rooting Success: Lovastatin Unveils Cytokinin Inhibition in Malus prunifolia var. ringo Apple Stem Cuttings. Horticulturae. 2023; 9(12):1341. https://doi.org/10.3390/horticulturae9121341
Chicago/Turabian StyleSun, Sinuo, Muhammad Mobeen Tahir, Zushu Xie, Pengyan Wei, Jianing Yu, Hangkong Liu, Yinnan He, Xiaoying Ren, Yuanyuan Ma, and Jiangping Mao. 2023. "Balancing Hormones and Gene Expressions for Rooting Success: Lovastatin Unveils Cytokinin Inhibition in Malus prunifolia var. ringo Apple Stem Cuttings" Horticulturae 9, no. 12: 1341. https://doi.org/10.3390/horticulturae9121341