Identification and Expression Analysis of CKX Gene Family in Brassica juncea var. tumida and Their Functional Analysis in Stem Development
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification of CKX Gene Family Members in B. juncea
2.2. Sequence Analysis and Phylogenetic Tree Construction
2.3. Chromosomal Localization and Collinearity Analysis
2.4. Analysis of Gene Structure, Conserved Motifs and Cis-Acting Elements in Promoter Region
2.5. Expression Pattern Analysis of CKX Genes in B. juncea var. tumida
2.6. Plant Materials, RNA Isolation and cDNA Synthesis, and Real-Time Quantitative PCR (RT-qPCR) Validation
2.7. Hormone Extraction and Purification
3. Results
3.1. Identification and Analysis of Physicochemical Properties of CKX Gene Family Members in B. juncea
3.2. Phylogenetic Analysis of CKX Gene Family
3.3. Analysis of Chromosome Localization and Gene Replication
3.4. Collinearity Analysis
3.5. Analysis of Gene Structure and Conserved Motifs
3.6. Analysis of Promoter Cis-Acting Elements
3.7. Transcriptional Expression Analysis of CKX Gene during Tumorous Stem Development
3.8. RT-qPCR Validation of CKX Gene in B. juncea var. tumida during Stem Formation
3.9. Dynamic Changes in Hormone Levels during Stem Development
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Accession Number | Amino Acids | Molecular Weight/Da | Isoelectric Point | Protein Hydrophobicity | Subgroup | Subcellular Localization |
---|---|---|---|---|---|---|---|
BjuCKX01 | BjuB029649 | 505 | 56,097.41 | 5.75 | −0.019 | I | chlo/- |
BjuCKX02 | BjuA046407 | 450 | 49,748.58 | 5.17 | −0.055 | I | chlo/- |
BjuCKX03 | BjuA017172 | 502 | 55,439.79 | 5.63 | 0.019 | I | extr/- |
BjuCKX04 | BjuB028610 | 504 | 55,806.39 | 6.23 | 0.024 | I | plas/- |
BjuCKX05 | BjuB046928 | 524 | 58,189.70 | 5.78 | 0.016 | I | chlo/- |
BjuCKX06 | BjuA013849 | 524 | 57,978.45 | 5.54 | 0.030 | I | chlo/- |
BjuCKX07 | BjuO004995 | 515 | 58,506.51 | 5.62 | −0.220 | I | vacu/- |
BjuCKX08 | BjuO009289 | 519 | 59,054.18 | 5.88 | −0.215 | I | vacu/- |
BjuCKX09 | BjuA006752 | 1337 | 148,490.79 | 6.00 | −0.214 | I | nucl/- |
BjuCKX10 | BjuA016599 | 411 | 46,156.67 | 6.81 | −0.201 | I | cyto = vacu/- |
BjuCKX11 | BjuA006478 | 470 | 51,673.56 | 5.44 | −0.183 | IV | cyto/- |
BjuCKX12 | BjuO011542 | 534 | 59,220.25 | 5.21 | −0.126 | IV | cyto/- |
BjuCKX13 | BjuA038850 | 523 | 57,967.61 | 4.94 | −0.136 | IV | cyto/- |
BjuCKX14 | BjuO008975 | 525 | 57,914.52 | 4.97 | −0.127 | IV | cyto/- |
BjuCKX15 | BjuB029847 | 533 | 59,905.92 | 5.69 | −0.249 | V | vacu/- |
BjuCKX16 | BjuA029462 | 535 | 60,082.13 | 5.56 | −0.241 | V | E.R./- |
BjuCKX17 | BjuA029463 | 333 | 36,584.35 | 5.15 | −0.074 | V | E.R./- |
BjuCKX18 | BjuO000929 | 507 | 57,217.29 | 6.69 | −0.257 | VI | vacu/- |
BjuCKX19 | BjuA017827 | 535 | 60,758.18 | 7.14 | −0.260 | VI | vacu = golo/- |
BjuCKX20 | BjuB027127 | 549 | 62,108.95 | 8.80 | −0.259 | VI | mito/- |
BjuCKX21 | BjuA017334 | 548 | 61,990.78 | 9.10 | −0.247 | VI | mito/- |
BjuCKX22 | BjuB017009 | 526 | 59,226.41 | 7.02 | −0.237 | VI | chlo/- |
BjuCKX23 | BjuA010583 | 545 | 61,374.01 | 8.72 | −0.191 | VI | mito/- |
Period | GA3 (ng/g·FW) | ZR (ng/g·FW) | IAA (ng/g·FW) | ABA (ng/g·FW) | BR (ng/g·FW) | MeJA (ng/g·FW) |
---|---|---|---|---|---|---|
S1 | 6.601 ± 0.816 a | 12.191 ± 1.197 a | 62.815 ± 5.003 a | 55.976 ± 3.521 b | 8.654 ± 0.656 a | 16.922 ± 1.084 a |
S2 | 4.718 ± 0.264 b | 4.870 ± 0.728 b | 58.301 ± 7.164 a | 53.812 ± 2.329 b | 7.364 ± 0.396 b | 12.235 ± 0.901 b |
S3 | 4.471 ± 0.229 b | 2.915 ± 0.329 c | 47.196 ± 5.811 b | 84.153 ± 7.329 a | 6.904 ± 0.310 b | 13.975 ± 1.131 b |
S4 | 4.053 ± 0.309 b | 1.817 ± 0.219 c | 38.476 ± 2.237 b | 25.348 ± 2.466 c | 5.272 ± 0.464 c | 13.871 ± 1.026 b |
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Li, M.; Zhou, J.; Gong, L.; Zhang, R.; Wang, Y.; Wang, C.; Du, X.; Luo, Y.; Zhang, Y.; Wang, X.; et al. Identification and Expression Analysis of CKX Gene Family in Brassica juncea var. tumida and Their Functional Analysis in Stem Development. Horticulturae 2022, 8, 705. https://doi.org/10.3390/horticulturae8080705
Li M, Zhou J, Gong L, Zhang R, Wang Y, Wang C, Du X, Luo Y, Zhang Y, Wang X, et al. Identification and Expression Analysis of CKX Gene Family in Brassica juncea var. tumida and Their Functional Analysis in Stem Development. Horticulturae. 2022; 8(8):705. https://doi.org/10.3390/horticulturae8080705
Chicago/Turabian StyleLi, Mengyao, Jin Zhou, Li Gong, Ran Zhang, Yan Wang, Chao Wang, Xiaoming Du, Ya Luo, Yong Zhang, Xiaorong Wang, and et al. 2022. "Identification and Expression Analysis of CKX Gene Family in Brassica juncea var. tumida and Their Functional Analysis in Stem Development" Horticulturae 8, no. 8: 705. https://doi.org/10.3390/horticulturae8080705