Transcriptomic Analysis of Sunflower (Helianthus annuus) Roots Resistance to Orobanche cumana at the Seedling Stage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Materials
2.2. Sampling Method
2.3. Library Preparation for Transcriptome Sequencing
2.4. Clustering and Sequencing
2.5. Quality Control and Mapping Reads to the Reference Genome
2.6. Quantification of Gene Expression Level
2.7. Differential Expression Analysis
2.8. GO and KEGG Enrichment Analysis of DEGs
2.9. qPCR
3. Results
3.1. O. cumana Parasitism of Sunflower
3.2. Analysis of Overall Sequencing Data Quality
3.3. Sunflower Differentially Expressed Genes following Infection with O. cumana
3.4. Gene Expression Patterns and Pathway Enrichment during Sunflower–O. cumana Interaction
3.5. Transcriptomic Differences between HZ2399 and SQ25
3.6. Confirmation of Key Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Test Number | Name of Material | Sampling Time | Number of Replication |
---|---|---|---|
KC1 | HZ2399 ♀ | Interaction with O. cumana for 0 h | Replication Ⅰ |
KC2 | HZ2399 ♀ | Interaction with O. cumana for 0 h | Replication Ⅱ |
KC3 | HZ2399 ♀ | Interaction with O. cumana for 0 h | Replication Ⅲ |
KPA1 | HZ2399 ♀ | Interaction with O. cumana for 4 h | Replication Ⅰ |
KPA2 | HZ2399 ♀ | Interaction with O. cumana for 4 h | Replication Ⅱ |
KPA3 | HZ2399 ♀ | Interaction with O. cumana for 4 h | Replication Ⅲ |
KPB1 | HZ2399 ♀ | Interaction with O. cumana for 12 h | Replication Ⅰ |
KPB2 | HZ2399 ♀ | Interaction with O. cumana for 12 h | Replication Ⅱ |
KPB3 | HZ2399 ♀ | Interaction with O. cumana for 12 h | Replication Ⅲ |
KPC1 | HZ2399 ♀ | Interaction with O. cumana for 24 h | Replication Ⅰ |
KPC2 | HZ2399 ♀ | Interaction with O. cumana for 24 h | Replication Ⅱ |
KPC3 | HZ2399 ♀ | Interaction with O. cumana for 24 h | Replication Ⅲ |
KPD1 | HZ2399 ♀ | Interaction with O. cumana for 48 h | Replication Ⅰ |
KPD2 | HZ2399 ♀ | Interaction with O. cumana for 48 h | Replication Ⅱ |
KPD3 | HZ2399 ♀ | Interaction with O. cumana for 48 h | Replication Ⅲ |
KPE1 | HZ2399 ♀ | Interaction with O. cumana for 72 h | Replication Ⅰ |
KPE2 | HZ2399 ♀ | Interaction with O. cumana for 72 h | Replication Ⅱ |
KPE3 | HZ2399 ♀ | Interaction with O. cumana for 72 h | Replication Ⅲ |
GC1 | SQ25 | Interaction with O. cumana for 0 h | Replication Ⅰ |
GC2 | SQ25 | Interaction with O. cumana for 0 h | Replication Ⅱ |
GC3 | SQ25 | Interaction with O. cumana for 0 h | Replication Ⅲ |
MPA1 | SQ25 | Interaction with O. cumana for 4 h | Replication Ⅰ |
MPA2 | SQ25 | Interaction with O. cumana for 4 h | Replication Ⅱ |
MPA3 | SQ25 | Interaction with O. cumana for 4 h | Replication Ⅲ |
MPB1 | SQ25 | Interaction with O. cumana for 12 h | Replication Ⅰ |
MPB2 | SQ25 | Interaction with O. cumana for 12 h | Replication Ⅱ |
MPB3 | SQ25 | Interaction with O. cumana for 12 h | Replication Ⅲ |
MPC1 | SQ25 | Interaction with O. cumana for 24 h | Replication Ⅰ |
MPC2 | SQ25 | Interaction with O. cumana for 24 h | Replication Ⅱ |
MPC3 | SQ25 | Interaction with O. cumana for 24 h | Replication Ⅲ |
MPD1 | SQ25 | Interaction with O. cumana for 48 h | Replication Ⅰ |
MPD2 | SQ25 | Interaction with O. cumana for 48 h | Replication Ⅱ |
MPD3 | SQ25 | Interaction with O. cumana for 48 h | Replication Ⅲ |
MPE1 | SQ25 | Interaction with O. cumana for 72 h | Replication Ⅰ |
MPE2 | SQ25 | Interaction with O. cumana for 72 h | Replication Ⅱ |
MPE3 | SQ25 | Interaction with O. cumana for 72 h | Replication Ⅲ |
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Huang, Q.; Lei, Z.; Xiang, L.; Zhang, W.; Zhang, L.; Gao, Y. Transcriptomic Analysis of Sunflower (Helianthus annuus) Roots Resistance to Orobanche cumana at the Seedling Stage. Horticulturae 2022, 8, 701. https://doi.org/10.3390/horticulturae8080701
Huang Q, Lei Z, Xiang L, Zhang W, Zhang L, Gao Y. Transcriptomic Analysis of Sunflower (Helianthus annuus) Roots Resistance to Orobanche cumana at the Seedling Stage. Horticulturae. 2022; 8(8):701. https://doi.org/10.3390/horticulturae8080701
Chicago/Turabian StyleHuang, Qixiu, Zhonghua Lei, Lijun Xiang, Wangfeng Zhang, Li Zhang, and Yan Gao. 2022. "Transcriptomic Analysis of Sunflower (Helianthus annuus) Roots Resistance to Orobanche cumana at the Seedling Stage" Horticulturae 8, no. 8: 701. https://doi.org/10.3390/horticulturae8080701